CN1425387A - Hydrolytic extrating method for medicinal component of pilose antler - Google Patents
Hydrolytic extrating method for medicinal component of pilose antler Download PDFInfo
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- CN1425387A CN1425387A CN03110823A CN03110823A CN1425387A CN 1425387 A CN1425387 A CN 1425387A CN 03110823 A CN03110823 A CN 03110823A CN 03110823 A CN03110823 A CN 03110823A CN 1425387 A CN1425387 A CN 1425387A
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- Prior art keywords
- cornu cervi
- cervi pantotrichum
- pilose antler
- hydrolysis
- hydrolytic
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The hydrolytic extracting process of medicinal component of pilose antler with fresh pilose antler includes mixing with buffering liquid, mincing in tissue mincing machine, pH regulation to 1.0-3.0, temperature regulation to 20-30 deg.c, hydrolysis while adding compound biological catalyst, centrifuging or filtering with diatomite to obtain clear golden pilose antler hydrolysate. After hydrolysis, about 70% matter in pilose antler is hydrolyzed and all the organic components become solution easy to absorb. The hydrolyate may be used in producing medicine, health article and food.
Description
Technical field:
The present invention relates to the extracting method of effective ingredient in Chinese composition, a kind of hydrolysis extracting method and product of Cornu Cervi Pantotrichum medicinal ingredient especially is provided, belong to Chinese medicine extracts active ingredients technical field.
Background technology:
Though Cornu Cervi Pantotrichum has medical value and stronger health-care effect preferably, because the organizational structure uniqueness of Cornu Cervi Pantotrichum-studies show that by the Cornu Cervi Pantotrichum organizational structure Cornu Cervi Pantotrichum (except that fine and soft skin) is to be made of the different osseous tissue of development degree from the top to the base portion; Therefore contain the material-chondroitin sulfate and the collagen fiber of a large amount of formation bones and prebone in the Cornu Cervi Pantotrichum, therefore Cornu Cervi Pantotrichum is not had the good method of utilizing; In traditional traditional medical, Cornu Cervi Pantotrichum can only carry out decocting as the part of drug matching, and utilization rate has only about 2% in utilizing process, and the overwhelming majority is all discarded; And as traditional tonic, generally can only be used to steep in wine etc., utilization rate is then lower.Therefore, traditional Cornu Cervi Pantotrichum utilize mode, be very big waste to the Cornu Cervi Pantotrichum resource.
Summary of the invention:
The invention provides a kind of hydrolysis extracting method and product of Cornu Cervi Pantotrichum medicinal ingredient, make full use of valuable Cornu Cervi Pantotrichum resource, better bring into play the medical value of Cornu Cervi Pantotrichum.
Technical solution of the present invention may further comprise the steps:
Get fresh Cornu Cervi Pantotrichum and add buffer, with organizing pulverizer to rub, transferring the pH value of solution is 1.0-10.0, press 0.005～0.01 of Cornu Cervi Pantotrichum weight and add biocatalyzer, comprise: one or more in pepsin, HAP alkaline protease, papain, pancreatin, alkaline endo protease (Alcalase), six kinds of enzymes of food flavor enzyme (Flavourzyme) add Ca simultaneously
2+, Mg
2+, Zn
2+, Mg
2+Deng the chloride zymoexciter, 20 ℃-60 ℃ of temperature, the reaction 2 hours-5 hours that is hydrolyzed under the pH1.0-pH10.0 condition, centrifugal then or diatomite filtration obtains clarifying flavous Cornu Cervi Pantotrichum hydrolyzed solution, and percent hydrolysis is at 60%-70%.
Buffer: phosphate, citrate, lactate buffer.
Biocatalyzer comprises: pepsin, HAP alkaline protease, papain, pancreatin, alkaline endo protease (Alcalase), food flavor enzyme (Flavourzyme).
Activator: Ca
2+, Mg
2+, Zn
2+, Mg
2+Deng chloride.
Contain multiple active substance in the hydrolyzed solution: by analysis, contain plurality of active ingredients such as a large amount of micromolecule polypeptides, lipid (mainly containing phospholipid, steroidal compounds, fatty acid, neutral fat and prostaglandin etc.), free amino acid, trace element, saccharide in the solution after the Cornu Cervi Pantotrichum hydrolysis.
Good effect of the present invention is: after bright Cornu Cervi Pantotrichum was handled through method for hydrolysis, its average hydrolysis rate reached (percent hydrolysis does not coexist between the 60-75% according to the quality of bright Cornu Cervi Pantotrichum) about 70%; Residue after the hydrolysis is the part and the part fur of Cornu Cervi Pantotrichum calcification, and all organic matters substantially all become solution state, and effective ingredient nearly all in the Cornu Cervi Pantotrichum has all become the solution state that is easy to absorption of human body.The product that obtains can be applicable to all many-sides of pharmacy, health product, food, and it is extremely convenient to use, and utilizes present technique that the Cornu Cervi Pantotrichum product is developed a lot of new development approachs of can deriving simultaneously, obtains flanker.
The specific embodiment:
Embodiment 1
Get the Cornu Cervi Pantotrichum 1kg of certain quantity of fresh, add the phosphatic buffer of 3000ml, with organizing pulverizer to rub, add biocatalyzer: pepsin 10g, activator zinc chloride 0.1g, adjust pH is 1.5, and the temperature of solution is transferred to 25 ℃, puts into retort reaction 4 hours, the centrifugal residue that goes is collected transparent clarifying liquid 3200ml.
Embodiment 2:
Get the Cornu Cervi Pantotrichum 1kg of certain quantity of fresh, the buffer that adds the 3000ml citrate, with organizing pulverizer to rub, add biocatalyzer: papain 8g, activator calcium chloride 0.08g, adjust pH is 7.0, and the temperature of solution is transferred to 37 ℃, puts into retort reaction 5 hours, the centrifugal residue that goes is collected transparent clarifying liquid 3300ml.
Embodiment 3
Get the Cornu Cervi Pantotrichum 1kg of certain quantity of fresh, add the Lactated buffer of 3000ml, with organizing pulverizer to rub, add biocatalyzer: pancreatin 10g, activator manganese chloride 0.15g, adjust pH is 9.0, and the temperature of solution is transferred to 52 ℃, puts into retort reaction 3 hours, the centrifugal residue that goes is collected transparent clarifying liquid 3340ml.
Claims (4)
1, a kind of hydrolysis extracting method of Cornu Cervi Pantotrichum medicinal ingredient may further comprise the steps and finishes: get fresh Cornu Cervi Pantotrichum and add buffer, with organizing the pulverizer rubbing, add biocatalyzer and chloride activator in proportion, 20 ℃-60 ℃ of temperature; PH1.0-pH10.0; Be hydrolyzed under the condition and reacted centrifugal then or diatomite filtration 2 hours-5 hours.
2, method according to claim 1 is characterized in that: described biocatalyzer comprises one or more in total pepsin, HAP alkaline protease, papain, pancreatin, alkaline endo protease, the food flavor enzyme.
3, method according to claim 1 is characterized in that: described activator is Ca
2+, Mg
2+, Zn
2+, Mg
2+Chloride.
4, the product of producing according to the described method of claim 1～3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031108237A CN1193770C (en) | 2003-01-09 | 2003-01-09 | Hydrolytic extrating method for medicinal component of pilose antler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031108237A CN1193770C (en) | 2003-01-09 | 2003-01-09 | Hydrolytic extrating method for medicinal component of pilose antler |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1425387A true CN1425387A (en) | 2003-06-25 |
| CN1193770C CN1193770C (en) | 2005-03-23 |
Family
ID=4790097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031108237A Expired - Fee Related CN1193770C (en) | 2003-01-09 | 2003-01-09 | Hydrolytic extrating method for medicinal component of pilose antler |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1193770C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300324C (en) * | 2003-12-12 | 2007-02-14 | 长春工业大学 | Method for extraction and purification of polysaccharide from pilose antler |
| CN101948439A (en) * | 2010-08-17 | 2011-01-19 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN101084925B (en) * | 2007-06-22 | 2011-08-10 | 王蓓蕾 | Technology for producing pilose antler mother solution |
| CN102286561A (en) * | 2011-07-11 | 2011-12-21 | 长春工业大学 | Method for extracting active ingredients of fresh pilos antler through enzymatic degradation |
| CN101647568B (en) * | 2009-09-03 | 2012-09-05 | 辽宁鹿源参茸饮片有限公司 | Spotted deer antler liquid and preparation method thereof |
-
2003
- 2003-01-09 CN CNB031108237A patent/CN1193770C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300324C (en) * | 2003-12-12 | 2007-02-14 | 长春工业大学 | Method for extraction and purification of polysaccharide from pilose antler |
| CN101084925B (en) * | 2007-06-22 | 2011-08-10 | 王蓓蕾 | Technology for producing pilose antler mother solution |
| CN101647568B (en) * | 2009-09-03 | 2012-09-05 | 辽宁鹿源参茸饮片有限公司 | Spotted deer antler liquid and preparation method thereof |
| CN101948439A (en) * | 2010-08-17 | 2011-01-19 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN101948439B (en) * | 2010-08-17 | 2012-08-22 | 刘平 | Extraction method and application to medicine of active alkaloid compounds in cervus nippon temminck |
| CN102286561A (en) * | 2011-07-11 | 2011-12-21 | 长春工业大学 | Method for extracting active ingredients of fresh pilos antler through enzymatic degradation |
| CN102286561B (en) * | 2011-07-11 | 2015-05-13 | 长春工业大学 | Method for extracting active ingredients of fresh pilos antler through enzymatic degradation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1193770C (en) | 2005-03-23 |
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