CN101007020A - Composition of a cartilage protein and its application in medical treatment and health protection - Google Patents
Composition of a cartilage protein and its application in medical treatment and health protection Download PDFInfo
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- CN101007020A CN101007020A CNA2006100039384A CN200610003938A CN101007020A CN 101007020 A CN101007020 A CN 101007020A CN A2006100039384 A CNA2006100039384 A CN A2006100039384A CN 200610003938 A CN200610003938 A CN 200610003938A CN 101007020 A CN101007020 A CN 101007020A
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Abstract
The invention includes the components of a kind of natural chondroprotein compound as well as its application in the medical care, especially its application in treating joint disease and joint medical care. After peroral administration of the product, it has the effects of eliminate inflammation, relieving pain and strengthen the motor function without no toxic and side effects.
Description
Technical field:
The present invention includes a kind of composition of natural cartilage albumen composition, and the application of this complex aspect health care, the especially application aspect treatment joint disease and health care joint.
Background technology:
Osteoarthritis is the modal a kind of diseases of person in middle and old age.According to statistics, in the middle-aged and elderly people more than 40 years old, about people more than 90% has osteoarthritis in various degree.Symptomatic arthritis is height with weight-bearing joint lumbar vertebra, cervical vertebra, knee joint incidence rate.Osteoarthritis is one of major reason of disabling of old people's health.Traditionally, generally come treatment of arthritis with antiinflammatory or anaesthetic.But these chemical synthetic drugs can only play the effect that slows down symptom, and can not control and stop arthritic formation and development.And they also have serious adverse, for example the intestines and stomach damage, ulcer or hemorrhage, even increase cardiopathic incidence rate.Therefore, urgently need to seek more effective, and the little prevention of side effect and the method for treatment of arthritis.
Modern scientific research proves that osteoarthritis is caused by many factors.Although age growth is the major reason that causes osteoarthritis, the osteoarthritis old and feeble wearing and tearing in simple anything but joint.The generation of osteoarthritis and development are processes that very complicated biochemistry changes.In other words, from the angle of modern cartilage biology, joint threpsology and medical science, want really to stop effectively or slow down the generation and the development of osteoarthritis, then need to the various tissues of intraarticular, especially articular cartilage provides a large amount of and isostatic life nutrient substance.Be referred to as the articular cartilage protective agent on these material sciences, for example glucosamine, chondroitin sulfate, hyaluronic acid, two collagen types, vitamin and micro-mineral element etc.
Ocean cavity biology, for example Stichopus japonicus and Rhopilema esculenta all contain the nutrition of abundant needed by human body.Traditional medicine is used for the treatment of gout, arthritis, tracheitis, hypertension and skin care etc. to these ocean cavity biologies.Modern science proves that these ocean cavity biologies contain the extremely abundant material that is similar to biological cartilage composition.For example Stichopus japonicus contains abundant class chondroitin sulfate, and Rhopilema esculenta contains extremely abundant class II collagen type.In addition; the Stichopus japonicus body contains more than the 50 kind of material useful to the human physiological activity; wherein protein content is up to more than 55%; 18 seed amino acids, taurine, the multiple composition of Radix Morinae Bulleyanae mucopolysaccharide, multivitamin such as element such as calcium, phosphorus, ferrum, iodine, zinc, selenium, vanadium, manganese and vitamin B1, vitamin B2, nicotinic acid.In the 18 contained seed amino acids, there are 8 kinds to be that human body self can not synthetic essential amino acids.Also contain abundant mineral element in the Rhopilema esculenta body.
Modern scientific research shows that glucosamine, chondroitin sulfate or hyaluronic acid etc. can promote the synthetic of biological cartilaginous tissue, and collagen hydrolysate also can promote chondrocyte to synthesize the II collagen type.The II collagen type is the primary structure albumen of biological cartilage, and the health in joint is played an important role.These materials have also been illustrated in clinical research to be had certain modification and improves function the structure in joint, so there is the certain protection effect in the joint.Yet, being used at present to modify and to improve the pharmaceutical compositions of articulation structure single, Time of Administration is long, weak curative effect.For example, present commercially available glucosamine product, the time that generally need take at least 12 weeks just produces some effects.
In view of arthritis has become current harm humans; especially one of healthy important diseases of middle-aged and elderly people; undoubtedly, seek natural joint nutrition protection material more effective and that side effect is little from occurring in nature and the extremely harmful chemical synthetic drug of human body is just become a problem that urgently needs solution with replacement or minimizing use.
The present invention addresses that need exactly, according to the up-to-date scientific theory of modern cartilage biology, joint threpsology and medical science, and the uniqueness that design is developed and novel natural cartilage albumen composition.This natural cartilage albumen composition has comprised diversified natural joint protection material.Wherein protection material in each joint is not only brought into play its own unique effect, and they are also collaborative mutually and mutually promote arthritic prevention effect is strengthened more.What is more important; natural cartilage albumen composition of the present invention is made up of natural fine biological raw material entirely; the ratio of its contained multiple natural joint protection material is corresponding mutually with the chemical composition of human articular cartilage, for the proper motion function in joint provides all isostatic nutritional needs.Therefore, natural cartilage albumen composition of the present invention is not only to the healthy nutritious protective effect in joint, and to joint disease, for example gonarthritis and cervical spondylosis all have the obvious treatment effect, and have no side effect.
Summary of the invention:
The present invention includes a kind of composition of natural cartilage albumen composition, and the application of this complex aspect health care, the especially application aspect treatment joint disease and health care joint.The composition characteristic of this natural cartilage albumen composition is as follows:
This natural cartilage albumen composition is made up of animal cartilage hydrolysate and/or ocean cavity biological hydrolysis thing.Animal cartilage hydrolysate and ocean cavity biological hydrolysis thing can be by chemical hydrolysis for example alkali or acid hydrolysis, or by the biological enzyme hydrolysis hydrolysate that is prepared from of hydrolysis such as trypsin for example.The animal cartilage hydrolysate comprises the cartilage hydrolysate that for example derives from cattle, pig, fish or chicken etc.Ocean cavity biological hydrolysis owner will comprise the hydrolysate of Stichopus japonicus and/or Rhopilema esculenta etc.
The ratio of contained animal cartilage hydrolysate can be 0.001% to 99.999% in the composition of this natural cartilage albumen composition, preferably more than 80%; Contained ocean cavity biological hydrolysis thing can be 99.999% to 0.001% in the composition of this natural cartilage albumen composition, preferably is not less than 10%.
The main chemical composition of this natural cartilage albumen composition is protein and aminopolysaccharide.Wherein contain protein about 63%; Contain aminopolysaccharide, comprise hyaluronic acid, chondroitin sulfate etc. about 27%.
Another content of the present invention be exactly this natural cartilage albumen composition to joint disease, for example gonarthritis and cervical spondylosis etc. have the obvious treatment effect.With this chondroprotein complex, take for the joint disease patient with forms such as powder, tablet, capsule, pastes, can make patient's anti-inflammatory analgetic, strengthen and recover its motor function, and have no side effect.Concrete instructions of taking is: oral this natural cartilage albumen composition, and gram every day 1 to 10 (preferably every day, 4-5 restrained), (best 7 days) were a course of treatment in 5 to 10 days.To joint disease, for example gonarthritis and cervical spondylosis have tangible curative effect.
Example 1: with pigeon chest cartilage, Stichopus japonicus and Rhopilema esculenta is feedstock production natural cartilage albumen composition
Select healthy pigeon chest cartilage, Stichopus japonicus and Rhopilema esculenta for use, remove surface impurity by 15: 1: 2 mixed, add water and surpass material, the stirring and washing number minute bleeds off muddy water.Material is inserted in the sealed pressure vessel high-temperature steam steaming and decocting of feeding certain pressure (0.2-0.3MPa) 20 minutes.Drop is gone out material be ground into granular (as the grain size) with meat mincer, after the water that adds former drop liquid and 4-5 times of inventory then stirred, the reuse colloid mill was levigate with it, becomes thick suspension, emulsion.Above-mentioned material is inserted in the isothermal reaction jar, adds the 2-5% interpolation that trypsin is pressed material quantity), kept constant temperature (37 ± 1 ℃) 6-8 hour, stir once per half an hour.Reaction finishes speed and was warming up to boiling point (100 ℃) 10 minutes, and postcooling is to room temperature.Above-mentioned enzymatic hydrolysis reaction liquid is filtered by 360 mesh sieves,, use molecular weight to hold back specification 50,000 daltonian ultrafilter membranes then and carry out ultrafiltration and hold back, be lower than 50,000 daltonian chondroprotein complex thereby obtain molecular weight to remove plurality of impurities such as skeletal grain.Above-mentioned filtrate is squeezed in the concentration tank, and heating evaporation moisture makes it be condensed into rare paste.Above-mentioned plaster material is inserted in the roller drier material storing box, make it be dried to lamellar.Sheet material is pulverized with pulverizer, then it is inserted in the 60-70 ℃ of baking oven, continue drying under the convulsion condition, make moisture Control in 10%, pack then.Per 1000 gram raw materials can prepare about 110 gram chondroprotein complex.
Example 2: chondroprotein complex proteins matter Determination on content
The mensuration of protein content is the international prescriptive procedure-Kjeldahl according to protein determination in the chondroprotein complex.Precision takes by weighing sample 0.2-2.0g from the chondroprotein complex of batch production, uses Kjeldahl to measure Protein content in its sample.Measure calculating through the method, the Protein content of each batch chondroprotein complex is all about 63%.
Example 3: chondroprotein complex aminopolysaccharide Determination on content
Chondroprotein complex aminopolysaccharide Determination on content is the method delivered on U.S.'s analytical biochemistry magazine according to doctor Gold [(Gold, E.W.Analytical Biochemistry 99,183-188 (1979)].The method quick and favorable reproducibility easy and simple to handle is aminopolysaccharide (Glycosaminoglycan, GAG) one of assay method of extensive use in the world.
The preparation of titer: take by weighing 10 milligrams of commodity chondroitin sulfate, it is dissolved in distilled water, and be settled to 10 milliliters, promptly obtaining concentration is the standard substance mother solution of 1 mg/ml.Titer with standard substance mother solution dilution preparation variable concentrations.
The preparation of the blue solution of Alcian (1.4 mg/ml): take by weighing the blue 8XG of 70 milligrams of Alcian, it is dissolved in 50 milliliter of 0.05 molar acetate sodium solution.
The preparation of chondroprotein complex testing sample: precision takes by weighing 15 milligrams in sample from the chondroprotein complex of batch production, and it is dissolved in distilled water, and is settled to 10 milliliters, and promptly getting testing sample solution concentration is 1.50 mg/ml.
The preparation of standard curve and the mensuration of testing sample: respectively get the titer of 0.1 milliliter of variable concentrations and testing sample in vitro.In each test tube, add the blue solution of 1.2 milliliters of Alcian respectively, mix homogeneously, room temperature reacted 10 minutes down.Measure the optical absorption intensity of this reactant liquor then in 480 nanometers.Optical absorption intensity drawing standard curve according to the titer of variable concentrations.The aminopolysaccharide concentration of chondroprotein complex testing sample can be found from standard curve according to its optical absorption intensity in 480 nanometers.Gained as calculated, the content of aminopolysaccharide is all about 27% in each batch chondroprotein complex.
Example 4: the effect of chondroprotein complex treatment of arthritis
Arthritic's case 1, woman, 50 years old, knee joint is not only swollen but also painful about 5 years, and fatigue increases the weight of, and gets up morning and bends and stretches difficulty, send out stiff, finger is not only swollen but also painful, and getting up early dare not be clenched fist, and lives and does not all do, sometimes take kitchen knife all to take incessantly, pain unbearably, the knee joint of walking is sometimes fallen down once askew, and is difficult sleepingly often painful.This patient begins oral chondroprotein complex of the present invention, and restrain every days 5.After taking three, clinical symptoms is just obviously improved, pain relief, sleep can fall asleep.After continuing to take three weeks, above clinical symptom disappearance.The joint pneumonedema, not painful yet, can work normally.
Arthritic's case 2, man, 53 years old.Two knee joint pain are difficult on foot when working fatigue.Usually feel lighter, " click clatter " sound is arranged during crouching, right elbow bears a heavy burden painful.Hold and hammer into shape when working, often hammer is unconscious flies out from hands.Medical history 2 to 3 years.Begin to take chondroprotein complex of the present invention in July, 2004, every day three times, each 1.5 grams.After taking for two weeks, two knee joint clinical symptom disappearance.After around continuing to take, the elbow joint no longer pain of bearing a heavy burden.Visit 1 year surplus, clinical symptoms occurs again.
Arthritic's case 3, woman, 49 years old.In recent years, two knee joint pain of going upstairs, the snap of having squatted, Changes in weather influences very big to pain, winter particularly, the ache all over the body that snows of blowing.This patient begins to take chondroprotein complex of the present invention from July, 2004, every day three times, each 1.5 grams.After taking for five weeks, two knee joints are strong, the no longer pain of two knee joints of going upstairs, and Changes in weather also no longer includes influence.
Example 5: the effect of chondroprotein complex treatment cervical spondylosis
Cervical spondylosis patient's case 1, man, 64 years old.Patient's readme: before taking chondroprotein complex of the present invention, cervical region acid is expanded uncomfortable, and dizzy head is heavy, and especially the scalp face is felt numb, and eyeball is as will " emitting ".Evening, sleep was tossed about, and head does not know where to put better.Four, 5 years, more and more severe, ate good multiple medicines, used many folk prescriptions, effect is little, and the painful sense of fiber crops is arranged during wrist afterwards.Take chondroprotein complex of the present invention, every day three times, each 1.5 grams.After taking for three weeks, above-mentioned symptom disappears substantially, has a clear head, and a dizzy heavy feeling of numbness has not had, and eyes are comfortable, and sleep is also fragrant, takes for two weeks again, all transference cures.
Cervical spondylosis patient's case 2, man, 43 years old.Painful three, four years of cervical vertebra increases the weight of gradually.Before taking chondroprotein complex of the present invention, headache, dizzy head is heavy, feels numb.Daytime, the watching focus newspaper was thought in working, and not just giddy of a few minutes is felt bad really after taking up.Want to see TV evening, less than just can't stand dysphoria half an hour.Take chondroprotein complex of the present invention, every day three times, each 1.5 grams.After taking for five weeks, above-mentioned symptom disappears substantially, and neck is comfortable, and influence is not read newspaper, seen TV, just as the normal person at all.Took for three weeks again, to consolidate effect, a year and a half does not recur so far.
Cervical spondylosis patient's case 3, woman, 58 years old.Suffer from cervical spondylosis more than ten years, especially severe in this year.In addition, this patient has hypertension, and is fat, scalp swelling.During rotation, cervical region have " " sound, two retroauricular lymph nodes enlargements, it is bad to sleep, agitation is forgotten things well.Though clothes depressor, blood pressure are still fluctuated, extremely unstable, high pressure is between 180-230mmHg, and left lower limb has the numb pain sense.Order began to take chondroprotein complex of the present invention on July 24th, 2005, every day three times, each 1.5 grams.During taking,, withdraw other any anti-inflammatory and antalgic class medicine and Chinese patent medicine except that continuing to take the depressor.After taking a week, scalp swelling disappears, and sleep is improved.After around taking continuously, two retroauricular lymph nodes enlargements disappear, and sleep is good, and the blood pressure high pressure is stabilized in about 180mmHg, and is in high spirits, and cervical region rotates sound and disappears substantially, and left lower limb has not had the numb pain sense.Existing every day three times, each 1.5 grams are still taken to consolidate curative effect continuing.
Claims (7)
1. the composition of a natural cartilage albumen composition.
2. the natural cartilage albumen composition in the claim 1 is made up of animal cartilage hydrolysate and/or ocean cavity biological hydrolysis thing.
3. animal cartilage hydrolysate in the claim 2 and/or ocean cavity biological hydrolysis thing comprise chemical hydrolysis for example alkali or acid hydrolysis, or by the biological enzyme hydrolysis hydrolysate that is prepared from of hydrolysis such as trypsin for example.
4. the animal cartilage hydrolysate in the claim 2 comprises the cartilage hydrolysate that for example derives from cattle, pig, fish or chicken etc.Ocean cavity biological hydrolysis owner will comprise the hydrolysate of Stichopus japonicus and/or Rhopilema esculenta etc.
5. contain animal cartilage hydrolysate 0.001% to 99.999% in the composition of the natural cartilage albumen composition in the claim 1, contain ocean cavity biological hydrolysis thing 99.999% to 0.001%.
6. the main chemical composition feature of the natural cartilage albumen composition in the claim 1 is protein and aminopolysaccharide.Wherein contain protein about 63%; Contain aminopolysaccharide, comprise hyaluronic acid, chondroitin sulfate etc. about 27%.
7. the natural cartilage albumen composition in the claim 1 comprises the application and the using method in arthritis, cervical spondylosis etc. and joint care field in the treatment joint disease.
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| CNA2006100039384A CN101007020A (en) | 2006-01-23 | 2006-01-23 | Composition of a cartilage protein and its application in medical treatment and health protection |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518941A (en) * | 2012-11-29 | 2014-01-22 | 中国科学院海洋研究所 | Method for preparing jellyfish gonad hydrolyzed protein |
| CN106072575A (en) * | 2016-07-07 | 2016-11-09 | 无锡博优特生物科技有限公司 | A kind of nourishing tonic for sport and containing its sport nutrition |
| WO2021131104A1 (en) * | 2019-12-27 | 2021-07-01 | Suntory Holdings Limited | Composition containing peptide, production method thereof, and use of peptide |
-
2006
- 2006-01-23 CN CNA2006100039384A patent/CN101007020A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518941A (en) * | 2012-11-29 | 2014-01-22 | 中国科学院海洋研究所 | Method for preparing jellyfish gonad hydrolyzed protein |
| CN106072575A (en) * | 2016-07-07 | 2016-11-09 | 无锡博优特生物科技有限公司 | A kind of nourishing tonic for sport and containing its sport nutrition |
| WO2021131104A1 (en) * | 2019-12-27 | 2021-07-01 | Suntory Holdings Limited | Composition containing peptide, production method thereof, and use of peptide |
| CN115066252A (en) * | 2019-12-27 | 2022-09-16 | 三得利控股株式会社 | Peptide-containing composition, method for producing same, and use of peptide |
| JP7545479B2 (en) | 2019-12-27 | 2024-09-04 | サントリーホールディングス株式会社 | Compositions containing peptides, methods for producing same, and uses of peptides |
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