CN1418562A - Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material - Google Patents
Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material Download PDFInfo
- Publication number
- CN1418562A CN1418562A CN 02157955 CN02157955A CN1418562A CN 1418562 A CN1418562 A CN 1418562A CN 02157955 CN02157955 CN 02157955 CN 02157955 A CN02157955 A CN 02157955A CN 1418562 A CN1418562 A CN 1418562A
- Authority
- CN
- China
- Prior art keywords
- feed liquid
- soybean
- phytone
- soybean protein
- hydrolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 15
- 244000068988 Glycine max Species 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title claims description 9
- 108090000790 Enzymes Proteins 0.000 title claims description 8
- 102000004190 Enzymes Human genes 0.000 title claims description 8
- 239000001888 Peptone Substances 0.000 title abstract description 6
- 108010080698 Peptones Proteins 0.000 title abstract description 6
- 235000019319 peptone Nutrition 0.000 title abstract description 6
- 239000002994 raw material Substances 0.000 title abstract description 6
- 235000013311 vegetables Nutrition 0.000 title 1
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 16
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 claims abstract description 12
- 230000007062 hydrolysis Effects 0.000 claims abstract description 11
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000010298 pulverizing process Methods 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 20
- WHWDWIHXSPCOKZ-UHFFFAOYSA-N hexahydrofarnesyl acetone Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)=O WHWDWIHXSPCOKZ-UHFFFAOYSA-N 0.000 claims description 17
- 230000001079 digestive effect Effects 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 238000010438 heat treatment Methods 0.000 abstract description 3
- 238000001035 drying Methods 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 102000038379 digestive enzymes Human genes 0.000 abstract 1
- 108091007734 digestive enzymes Proteins 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 230000029087 digestion Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Abstract
In the production method of plant peptone, soybean is used as raw material, which includes the following steps: low-temp. defatting soybean, pulverizing, soaking in hot water, heating and heat-insulating for a certain time, sterilizing, regulating pH value and temp. at 45 deg.C-60 deg.C, adding a certain quantity of soybean protein digestive enzyme to make hydrolysis, regulating pH value, heating to 100 deg.C, heat-insulating for 5 min., filtering, removing residue, concentrating and drying so as to obtain the invented product powder preparation.
Description
(1) technical field
The present invention relates to a kind of peptone production method, specifically a kind of is the phytone production method of raw material with the soybean.
(2) background technology
Peptone is widely used in fields such as microorganism, pharmacy as culture medium.Phytone especially is the phytone of raw material with the soybean, because its special performances and cheap, excellent economy such as raw material is easy to get thereof, more and more receives publicity and payes attention at bioengineering field.But the soy peptone of using as reagent and the soy peptone of commercial grade, the domestic product supply the market that has not yet to see suitability for industrialized production.Through ISTIC Research ﹠ Traning Center's update search,, do not describe the main contents of this technology in detail though the biochemical factory in Shan County, Henan Province has reported the achievement with the Production by Enzymes phytone.For example, adopt which kind of protease, technological parameter as how.Do not see that it has the product supply the market yet.Application number is that 94112516 patent application discloses a phytone and production method thereof.This technology adopts is to solidify soybean protein with salt to add algin again and make phytone.But its complex manufacturing, the cost height, quality is low, does not therefore form suitability for industrialized production.
(3) summary of the invention:
Task of the present invention is that providing a kind of is the new method that raw material is produced phytone with the soybean, to improve yield and product quality, reduces production costs.
For finishing above-mentioned task, technical scheme of the present invention is as follows:
The present invention is to be the production method that material, enzyme method is produced phytone with the soybean.After soybean was low-temperature defatted, pulverizing, hot-water soak, salt were carried, and made to contain soy proteinaceous feed liquid.This feed liquid heating, insulation, sterilization.And then this feed temperature reduced to 45 ℃～60 ℃, regulate pH value to 7.5, add the hydrolysis of a certain amount of soybean protein digestive ferment, after keeping certain hour, regulating this material liquid PH value once more is 5.0, and heat temperature raising to 100 ℃ is incubated 5 minutes, after filtration, concentrate, dry, make the phytone powder.
During hydrolysis, the soybean protein digestive ferment that adds in the feed liquid is a papain, addition be in the feed liquid soybean protein weight content 4～5%.
During hydrolysis, the soybean protein digestive ferment that adds in the feed liquid is 1398 enzymes, addition be in the feed liquid soybean protein weight content 8%.
The present invention has following advantage and effect.
1, the present invention is owing to adopt new digestive ferment hydrolysis to soybean protein, so the product quality height, the yield height, and the reagent peptone reaches 10%, and the peptone of commercial grade can reach 48%.
2, Processes and apparatus of the present invention is simple, and easy operating is convenient to control and maintenance.
3, to produce the phytone cost low in the present invention.
(4) specific embodiment
Embodiment 1
Soybean through the screening, low-temperature defatted after, be crushed to certain fineness.In hot water, soak 80 ℃ of water temperatures, pH value 7.6 then.For improving the solubility of soybean protein, adding concentration in this feed liquid is the Nacl solution of 0.5～2mol/L.Soak filter cleaner after 2 hours, feed liquid is sent into the digestion hydrolytic tank.Regulate feed temperature to 60 ℃, pH value is 7.5.Add papain then.Addition be in the feed liquid soybean protein weight content 4～5%.The digestion hydrolysis time is 3 hours.Afterwards, regulate material liquid PH value to 5.0, heat temperature raising to 100 ℃ is incubated 5 minutes.Then after filtration, concentrate, dry, make the phytone pulvis.
Embodiment 2
Soybean is sent into the hydrolytic digestion pond to feed liquid after low-temperature defatted, pulverizing, hot-water soak, salt are mentioned filtration.Adjust feed temperature to 45 ℃, pH value is 7.5.Add 1398 enzyme hydrolysis digestion again.Addition be in the feed liquid soybean protein weight content 8%.The digestion hydrolysis time is 3 hours.After hydrolytic digestion finished, regulating material liquid PH value was 5.0, and heat temperature raising to 100 ℃ is incubated 5 minutes.And then the slagging-off filtration, make the phytone pulvis through concentrated, drying.
Claims (3)
1, a kind of is the method that material, enzyme method is produced phytone with the soybean, soybean low-temperature defatted after, the molten lixiviate of pulverizing, hot-water soak and salt, make and contain soy proteinaceous feed liquid, with this feed liquid heat sterilization, it is characterized in that, reducing to 45 ℃-60 ℃ through the feed temperature after the sterilization treatment, pH value is adjusted to 7.5, adds the hydrolysis of a certain amount of soybean protein digestive ferment, keeps 3 hours, then feed liquid heat temperature raising to 100 ℃ once more, be incubated 5 minutes, after filtration, concentrate, dry, make the phytone pulvis.
2, the method for production phytone according to claim 1, the soybean protein digestive ferment that adds in the feed liquid when it is characterized in that hydrolysis is a papain, addition is the 4-5% of soybean protein weight content in the feed liquid.
3, the method for production phytone according to claim 1 is characterized in that, the soybean protein digestive ferment that adds during hydrolysis is 1398 enzymes, addition be in the feed liquid soybean protein weight content 8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021579555A CN1185947C (en) | 2002-12-23 | 2002-12-23 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021579555A CN1185947C (en) | 2002-12-23 | 2002-12-23 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1418562A true CN1418562A (en) | 2003-05-21 |
| CN1185947C CN1185947C (en) | 2005-01-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021579555A Expired - Fee Related CN1185947C (en) | 2002-12-23 | 2002-12-23 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1185947C (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283310A (en) * | 2010-06-17 | 2011-12-21 | 靳群牛 | Method for producing polypeptone by taking casein as raw material through enzymolysis |
| CN102911983A (en) * | 2012-11-23 | 2013-02-06 | 海南椰国食品有限公司 | Application of carambola in biocellulose fermentation media |
| CN103898184A (en) * | 2014-04-09 | 2014-07-02 | 青岛博之源生物技术有限公司 | Method of preparing phytone by utilizing hot-pressed peanut meal |
| US9309444B2 (en) | 2009-03-06 | 2016-04-12 | Biopolymer Technologies, Ltd. | Protein-containing emulsions and adhesives, and manufacture and use thereof |
| US9416303B2 (en) | 2010-06-07 | 2016-08-16 | Biopolymer Technologies, Ltd. | Protein-containing adhesives, and manufacture and use thereof |
| CN106350559A (en) * | 2016-10-17 | 2017-01-25 | 保定味群食品科技股份有限公司 | Method for producing phytone from vital wheat gluten |
| US9873823B2 (en) | 2012-07-30 | 2018-01-23 | Evertree | Protein adhesives containing an anhydride, carboxylic acid, and/or carboxylate salt compound and their use |
| US10125295B2 (en) | 2011-09-09 | 2018-11-13 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| US10160842B2 (en) | 2009-03-06 | 2018-12-25 | Evertree | Protein-containing foams, manufacture and use thereof |
| US11028298B2 (en) | 2011-09-09 | 2021-06-08 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911995B (en) * | 2012-11-12 | 2014-05-07 | 张喜胜 | Extraction method of wheat peptone |
-
2002
- 2002-12-23 CN CNB021579555A patent/CN1185947C/en not_active Expired - Fee Related
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9309444B2 (en) | 2009-03-06 | 2016-04-12 | Biopolymer Technologies, Ltd. | Protein-containing emulsions and adhesives, and manufacture and use thereof |
| US10745601B2 (en) | 2009-03-06 | 2020-08-18 | Evertree | Protein-containing emulsions and adhesives, and manufacture and use thereof |
| US10160842B2 (en) | 2009-03-06 | 2018-12-25 | Evertree | Protein-containing foams, manufacture and use thereof |
| US9909044B2 (en) | 2009-03-06 | 2018-03-06 | Evertree | Protein-containing emulsions and adhesives, and manufacture and use thereof |
| US10465103B2 (en) | 2010-06-07 | 2019-11-05 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| US9416303B2 (en) | 2010-06-07 | 2016-08-16 | Biopolymer Technologies, Ltd. | Protein-containing adhesives, and manufacture and use thereof |
| US9816019B2 (en) | 2010-06-07 | 2017-11-14 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| US10913880B2 (en) | 2010-06-07 | 2021-02-09 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| CN102283310A (en) * | 2010-06-17 | 2011-12-21 | 靳群牛 | Method for producing polypeptone by taking casein as raw material through enzymolysis |
| US11072731B2 (en) | 2011-09-09 | 2021-07-27 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| US11028298B2 (en) | 2011-09-09 | 2021-06-08 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| US10125295B2 (en) | 2011-09-09 | 2018-11-13 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| US9873823B2 (en) | 2012-07-30 | 2018-01-23 | Evertree | Protein adhesives containing an anhydride, carboxylic acid, and/or carboxylate salt compound and their use |
| US10526516B2 (en) | 2012-07-30 | 2020-01-07 | Evertree | Protein adhesives containing an anhydride, carboxylic acid, and/or carboxylate salt compound and their use |
| CN102911983A (en) * | 2012-11-23 | 2013-02-06 | 海南椰国食品有限公司 | Application of carambola in biocellulose fermentation media |
| CN102911983B (en) * | 2012-11-23 | 2014-07-09 | 海南椰国食品有限公司 | Application of carambola in biocellulose fermentation media |
| CN103898184A (en) * | 2014-04-09 | 2014-07-02 | 青岛博之源生物技术有限公司 | Method of preparing phytone by utilizing hot-pressed peanut meal |
| CN106350559A (en) * | 2016-10-17 | 2017-01-25 | 保定味群食品科技股份有限公司 | Method for producing phytone from vital wheat gluten |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1185947C (en) | 2005-01-26 |
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Granted publication date: 20050126 Termination date: 20121223 |