CN1185947C - Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material - Google Patents
Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material Download PDFInfo
- Publication number
- CN1185947C CN1185947C CNB021579555A CN02157955A CN1185947C CN 1185947 C CN1185947 C CN 1185947C CN B021579555 A CNB021579555 A CN B021579555A CN 02157955 A CN02157955 A CN 02157955A CN 1185947 C CN1185947 C CN 1185947C
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- China
- Prior art keywords
- feed liquid
- soybean protein
- phytone
- material liquid
- soybean
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 16
- 244000068988 Glycine max Species 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 8
- 239000001888 Peptone Substances 0.000 title abstract description 8
- 108010080698 Peptones Proteins 0.000 title abstract description 8
- 235000019319 peptone Nutrition 0.000 title abstract description 8
- 239000002994 raw material Substances 0.000 title abstract description 6
- 235000013311 vegetables Nutrition 0.000 title abstract 4
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 17
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 3
- WHWDWIHXSPCOKZ-UHFFFAOYSA-N hexahydrofarnesyl acetone Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)=O WHWDWIHXSPCOKZ-UHFFFAOYSA-N 0.000 claims description 17
- 230000007062 hydrolysis Effects 0.000 claims description 10
- 238000006460 hydrolysis reaction Methods 0.000 claims description 10
- 230000001079 digestive effect Effects 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 4
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000012423 maintenance Methods 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract 2
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 230000013777 protein digestion Effects 0.000 abstract 1
- 230000029087 digestion Effects 0.000 description 6
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention discloses a method for producing vegetable peptone by an enzyme method using soybeans as raw materials, which is suitable for the production of vegetable peptone used for fermentation industry, biologic pharmaceutical industry, etc. The method comprises the following steps: after the soybeans are defatted at a low temperature, crushed and soaked in hot water, the soybeans are heated for heat preservation for certain time and are sterilized; afterwards, the pH value of material liquid is adjusted, the temperature of the material liquid is raised to 45 DEG C to 60 DEG C, and a certain amount of soybean protein digestion enzyme is added to the material liquid to be hydrolyzed; after the hydrolyzation is carried out for certain time, the pH value of the material liquid is adjusted, and the temperature is raised to 100 DEG C; after the heat preservation is carried out for 5 minutes, the material liquid is processed by filtration, residue removal, concentration and drying to prepare vegetable peptone powder. The method has the advantages of simple technology and apparatus, easy operation, convenient control and maintenance, high product quality, high product yield and low production cost.
Description
(1) technical field
The present invention relates to a kind of peptone production method, specifically a kind of is the phytone production method of raw material with the soybean.
(2) background technology
Peptone is widely used in fields such as microorganism, pharmacy as culture medium.Phytone especially is the phytone of raw material with the soybean, because its special performances and cheap, excellent economy such as raw material is easy to get thereof, more and more receives publicity and payes attention at bioengineering field.But the soy peptone of using as reagent and the soy peptone of commercial grade, the domestic product supply the market that has not yet to see suitability for industrialized production.Through ISTIC Research ﹠ Traning Center's update search, though the biochemical factory in Shan County, Henan Province has reported the achievement with the Production by Enzymes phytone, do not describe the main contents of this technology in detail, for example, adopt which kind of protease, technological parameter as how.Do not see that it has the product supply the market yet.Application number is that 94112516 patent application discloses a phytone and production method thereof.This technology adopts is to solidify soybean protein with salt to add algin again and make phytone.But its complex manufacturing, the cost height, quality is low, does not therefore form suitability for industrialized production.
(3) summary of the invention:
The task of doing invention is that providing a kind of is the method that raw material is produced phytone with the soybean, to improve yield and product quality, reduces production costs.
For finishing above-mentioned task, technical scheme of the present invention is as follows:
The present invention is to be the production method that material, enzyme method is produced phytone with the soybean.After soybean was low-temperature defatted, pulverizing, hot-water soak and to add concentration be that the Nacl solution salt of 0.5-2MOl/L is carried were made and are contained soy proteinaceous feed liquid.This feed liquid heating, insulation, sterilization.And then this feed temperature reduced to 45 ℃-60 ℃, and regulate pH value to 7.5, add the hydrolysis of a certain amount of soybean protein digestive ferment, kept 3 hours.And then to regulate this material liquid PH value be 5.0, and heat temperature raising to 100 ℃ is incubated 5 minutes, after filtration, concentrate, dry, make the phytone powder.
During hydrolysis, the soybean protein digestive ferment that adds in the feed liquid is a papain, and addition is the 4-5% of soybean protein weight content in the feed liquid.
During hydrolysis, the soybean protein digestive ferment that adds in the feed liquid is 1398 neutral proteinases, addition be in the feed liquid soybean protein weight content 8%.
The present invention has following advantage and effect.
1, the present invention is owing to adopt new digestive ferment hydrolysis to soybean protein, so the product quality height, the yield height, and the reagent peptone reaches 10%, and the peptone of commercial grade can reach 48%.
2, Processes and apparatus of the present invention is simple, and easy operating is convenient to control and maintenance.
3, to produce the phytone cost low in the present invention.
(4) specific embodiment
Embodiment 1
Soybean through the screening, low-temperature defatted after, be crushed to certain fineness.In hot water, soak 80 ℃ of water temperatures, pH value 7.6 then.For improving the solubility of soybean protein, adding concentration in this feed liquid is the Nacl solution of 0.5-2mol/L.Soak filter cleaner after 2 hours, feed liquid is sent into the digestion hydrolytic tank.Regulate feed temperature to 60 ℃, pH value is 7.5.Add papain then.Addition is the 4-5% of soybean protein weight content in the feed liquid.The digestion hydrolysis time is 3 hours.Afterwards, regulate material liquid PH value to 5.0, heat temperature raising to 100 ℃ is incubated 5 minutes.Concentrate then after filtration,, dry, make the phytone pulvis.
Embodiment 2
Soybean is sent into the hydrolytic digestion pond to feed liquid after low-temperature defatted, pulverizing, hot-water soak, salt are mentioned filtration.Adjust feed temperature to 45 ℃, pH value is 7.5.Add the digestion of 1398 neutral proteinase hydrolysis again.Addition be in the feed liquid soybean protein weight content 8%.The digestion hydrolysis time is 3 hours.After hydrolytic digestion finished, regulating material liquid PH value was 5.0, and heat temperature raising to 100 ℃ is incubated 5 minutes.And then the slagging-off filtration, make the phytone pulvis through concentrated, drying.Used soybean protein digestive ferment is by the magnificent prosperous and powerful Bioisystech Co., Ltd in east, Beijing in the present embodiment, 1398 neutral proteinases that enterprises such as Wuxi enzyme preparation factory produce, and its specification is a solid, powdery.
Claims (3)
1, a kind of is the method that material, enzyme method is produced phytone with the soybean, soybean low-temperature defatted after, pulverize, hot-water soak, and adding concentration is the Nacl solution of 0.5-2M01/L, make and contain soy proteinaceous feed liquid, with this feed liquid heat sterilization, it is characterized in that, reducing to 45 ℃-60 ℃ through the feed temperature after the sterilization treatment, pH value is adjusted to 7.5, add the hydrolysis of a certain amount of soybean protein digestive ferment, kept 3 hours, regulate material liquid PH value to 5.0 then, feed liquid heat temperature raising to 100 ℃ once more, be incubated 5 minutes, after filtration, concentrate, drying is made the phytone agent.
2, the method for production phytone according to claim 1, the soybean protein digestive ferment that adds in the feed liquid when it is characterized in that hydrolysis is a papain, addition is the 4-5% of soybean protein weight content in the feed liquid.
3, the method for production phytone according to claim 1 is characterized in that, the soybean protein digestive ferment that adds during hydrolysis is 1398 neutral proteinases, addition be in the feed liquid soybean protein weight content 8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021579555A CN1185947C (en) | 2002-12-23 | 2002-12-23 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021579555A CN1185947C (en) | 2002-12-23 | 2002-12-23 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1418562A CN1418562A (en) | 2003-05-21 |
| CN1185947C true CN1185947C (en) | 2005-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021579555A Expired - Fee Related CN1185947C (en) | 2002-12-23 | 2002-12-23 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
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| Country | Link |
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| CN (1) | CN1185947C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911995A (en) * | 2012-11-12 | 2013-02-06 | 张喜胜 | Extraction method of wheat peptone |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012519760A (en) | 2009-03-06 | 2012-08-30 | バイオポリマー テクノロジーズ， リミテッド | Protein-containing foam, its manufacture and use |
| CA2753866C (en) | 2009-03-06 | 2020-04-28 | Biopolymer Technologies, Ltd. | Protein-containing emulsions and adhesives, and manufacture and use thereof |
| SI2576661T1 (en) | 2010-06-07 | 2017-05-31 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| CN102283310A (en) * | 2010-06-17 | 2011-12-21 | 靳群牛 | Method for producing polypeptone by taking casein as raw material through enzymolysis |
| CA3075143C (en) | 2011-09-09 | 2023-03-28 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| LT2753633T (en) | 2011-09-09 | 2017-04-10 | Evertree | Protein-containing adhesives, and manufacture and use thereof |
| WO2014020447A2 (en) | 2012-07-30 | 2014-02-06 | Biopolymer Tecnologies, Ltd. | Protein adhesives containing an anhydride, carboxylic acid, and/or carboxylate salt compound and their use |
| CN102911983B (en) * | 2012-11-23 | 2014-07-09 | 海南椰国食品有限公司 | Application of carambola in biocellulose fermentation media |
| CN103898184A (en) * | 2014-04-09 | 2014-07-02 | 青岛博之源生物技术有限公司 | Method of preparing phytone by utilizing hot-pressed peanut meal |
| CN106350559A (en) * | 2016-10-17 | 2017-01-25 | 保定味群食品科技股份有限公司 | Method for producing phytone from vital wheat gluten |
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2002
- 2002-12-23 CN CNB021579555A patent/CN1185947C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911995A (en) * | 2012-11-12 | 2013-02-06 | 张喜胜 | Extraction method of wheat peptone |
| CN102911995B (en) * | 2012-11-12 | 2014-05-07 | 张喜胜 | Extraction method of wheat peptone |
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| Publication number | Publication date |
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| CN1418562A (en) | 2003-05-21 |
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