CN102911983B - Application of carambola in biocellulose fermentation media - Google Patents
Application of carambola in biocellulose fermentation media Download PDFInfo
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- CN102911983B CN102911983B CN201210481105.4A CN201210481105A CN102911983B CN 102911983 B CN102911983 B CN 102911983B CN 201210481105 A CN201210481105 A CN 201210481105A CN 102911983 B CN102911983 B CN 102911983B
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Abstract
The invention relates to application of tropical fruit carambola in biocellulose fermentation media. The invention has the following beneficial effects: the application range of the carambola is expanded, and the subsidiary production value of the carambola is increased; meanwhile, biocellulose products with different tastes and impressions are obtained; and by adding special nitrogen sources, higher biocellulose yield and better characteristics are obtained, and the application range of the tropical fruit papayas is expanded. In short, the invention relates to a more economical and effective biocellulose production method.
Description
Technical field
The present invention relates to a kind of production method of biology cellulose.
Background technology
Biology cellulose (biocellulose, claim again bacteria cellulose, be called for short BC) typically refer under different condition, by synthetic cellulosic general designation such as certain microorganism in acetic acid Pseudomonas (Acetobacter), Agrobacterium (Agrobacterium), rhizobium (Rhizobium) and Sarcina (Sarcina) etc.Compared with natural cellulose, it has the advantageous properties such as high purity, high-crystallinity, high-polymerization degree, high retentiveness and high rehydration.Be widely used in every field.The production of biology cellulose, normally by microbial inoculants such as acetic acid Pseudomonas in being rich in the substratum of the nutritive ingredient such as nitrogenous source and carbon source, static fermentation 5-10 days at 20-30 ℃, can obtain on the surface of liquid culture medium translucent biology cellulose film.
Fermentation to produce biological Mierocrystalline cellulose usually adopts the main component of Sucus Cocois as substratum at present, although it is with low cost, be easy to application, but comparatively limitation of its source, supply is relatively little, along with the Application Areas of biology cellulose film product constantly expands, demand is also increasing, and the industrial scale of expanding day needs the raw material of more suitable for producing; And, need to provide the biology cellulose product that physical strength is larger in some field, and the product that adopts Sucus Cocois to obtain as fermention medium can not meet its demand.
Summary of the invention
The object of this invention is to provide the source of more biology cellulose fermentation substrate, be mainly utilize the most common plantation of tropical area and production scale all very large tropical fruit as the source of fermentation substrate.Both the scope that had expanded tropical fruit application, has increased its earning rate; Can supply in large quantity again biology cellulose fermentation required.
The present invention is mainly to provide the purposes of carambola in biology cellulose fermention medium, and in described fermention medium, containing the carambola fruit juice of weight percent 50 ~ 90%, described carambola fruit juice sugar degree is at the more than 10% of weight percent.
Carambola, formal name used at school carambola, has another name called " carambola ", " carambola ", is commonly called as " maple fruit " in South Fujian Province.Its square section is pentagram, therefore claim again abroad " star pears ".Carambola belongs to the torrid zone, south subtropics fruit, is evergreen dungarunga or shrub, originates in India, the state-owned plantations such as present Malaysia, Indonesia, and also there is cultivation in Hainan Province of China.Carambola is the fruit that a kind of moisture is a lot, and fresh fruit soluble solids is 9%.Carambola fresh fruit sugar degree ranks forefront in various fresh fruits, includes sucrose, fructose, glucose etc., also contains other VITAMIN and calcium, potassium, magnesium, micro-fat and protein etc., is the more comprehensive fruit of a kind of nutritive ingredient.In its 100 grams of fruit juice sugary 10 grams, 0.78 gram of acid, 0.72 gram, protein, 0.75 gram, fat, and the calcium of vitamin A, C and trace, iron, phosphorus etc., can be utilized by biology cellulose fermentative production bacterium completely, so set it as fermenting carbon source, it is extraordinary carbon source source.And VITAMIN and other inorganic elementss contained in carambola also can utilize for biology cellulose fermentative production bacteria growing, can improve better its fermentation efficiency.For the ease of using, the present invention is by after carambola peeling, and squeezing is concentrated makes carambola fruit juice, sets it as the main component of fermention medium, after regulating pH value, is directly used in fermentation to produce biological Mierocrystalline cellulose; Also can, again according to the physiological property of different fermentations bacterial classification, it be carried out to suitable allotment, as add appropriate nitrogenous source or inorganic elements etc., be used further to fermentation.
Carambola fruit juice for fermention medium of the present invention can prepare in advance in country of origin, then is transported to biology cellulose grown place; But higher for movement requirement like this; Preferably Carambola Fruit is transported to behind biology cellulose grown place, then preparation is used.
Carambola fruit juice for fermention medium of the present invention directly uses after can being concentrated on request certain sugar degree, and the biology cellulose of producing is pure transparent, and mouthfeel is sharp and clear, with light carambola fragrance.
Although contain compared with the inorganic elements of horn of plenty and sugar power deficiency, phenomenon that biology cellulose output is lower after the biology cellulose fermentation later stage can exist fermentation in carambola fruit juice.Find through research, major cause is the required nitrogenous source deficiency of fermented bacterium in fermenting process.So, the present invention further provides and be suitable for the required nitrogenous source source of biology cellulose fermentative production, it is vegetable protein peptone, described vegetable protein peptone is in vegetable-protein, to add Fructus Chaenomelis extract hydrolysis gained; Described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained after tannin precipitator method removal impurity.The preferred soybean protein of described vegetable-protein and/or gluten.Described vegetable protein peptone be in vegetable-protein, add Fructus Chaenomelis extract to be hydrolyzed and be dried to meal after obtain; Its molecular weight is 2000 left and right.
The present invention adopts vegetable-protein nitrogenous source source as biology cellulose fermented bacterium after Fructus Chaenomelis extract hydrolysis, and it is required to nitrogenous source that it can fully meet fermented bacterium, improves fermentation efficiency, improves tunning output; Can also make full use of the characteristic that this tropical fruit of pawpaw is rich in papoid; Because the preparation of described vegetable protein peptone has not only adopted vegetal dietary protein origin, also adopt vegetal proteolytic enzyme source, make in peptone protein decomposition efficiency higher, each seed amino acid and micromolecule polypeptide content are higher, and it is used that kind and character are more conducive to fermentation to produce biological Mierocrystalline cellulose, more can improve fermentation efficiency and product output; And be all food source, the food safety of the biology cellulose that fermentative production obtains is guaranteed.
On the basis of above-mentioned research, the invention provides a kind of biology cellulose fermention medium of excellence, the carambola fruit juice that it contains weight percent 50 ~ 90%, described carambola fruit juice sugar degree is at the more than 10% of weight percent; Also contain vegetable-protein and be hydrolyzed the vegetable protein peptone obtaining through Fructus Chaenomelis extract, described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained after tannin precipitator method removal impurity.
The biology cellulose output of utilizing above-mentioned fermention medium to ferment to obtain is made the fermention medium of main component biology cellulose output than Sucus Cocois can improve more than 8%.And toughness and the physical strength of product are also improved to some extent.
Above-mentioned fermention medium, wherein said vegetable-protein is soybean protein and/or gluten.
Above-mentioned fermention medium, wherein said vegetable protein peptone be in vegetable-protein, add Fructus Chaenomelis extract to be hydrolyzed and be dried to meal after obtain; Its molecular weight is 2000 left and right.
The pH value of above-mentioned fermention medium is 5.2-6.5.Can adopt citric acid or Glacial acetic acid to regulate.
The invention provides the application of tropical fruit carambola on biology cellulose fermention medium, expanded the range of application of carambola, increased its by-product value; Obtained the biology cellulose product of different mouthfeels and perception simultaneously; Obtain higher biology cellulose output and better characteristic through adding special nitrogenous source again, increased again the range of application of tropical fruit pawpaw.In general, be the more cost-effective biology cellulose production method of one.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1:
After the peeling of fresh carambola, squeezing is concentrated, makes 10% the carambola fruit juice of sugar degree at weight percent.Regulate pH5.8 with citric acid.The seed liquor of inoculation biology cellulose fermentative production bacterial classification, as acetobacter xylinum, static shallow tray fermentation 5 days, obtains membranaceous biology cellulose on the top layer of substratum at 30 ℃.Biology cellulose output is 6.7g/L.The Young's modulus of wet biology cellulose film is 5.24Gpa.Gained biology cellulose film is clear, colorless, news have light carambola fragrance.
Embodiment 2:
After the peeling of fresh carambola, squeezing is concentrated, makes 15% the carambola fruit juice of sugar degree at weight percent.
Gather immature papaya slurries, with filtering and make Fructus Chaenomelis extract after tannin precipitator method removal impurity.Described Fructus Chaenomelis extract is added in soybean protein, and water at normal temperature solution 1 day, makes soy peptone, and crushed after being dried makes the soy peptone that molecular weight is 2000 left and right.
Prepare fermention medium by following weight percent consumption:
The carambola fruit juice of sugar degree 15%: 60%;
Soy peptone: 10%;
Regulate pH5.2 with Glacial acetic acid.
Water: surplus.
To the seed liquor of inoculating biology cellulose fermentative production bacterial classification in the above-mentioned fermention medium preparing, as acetobacter xylinum, static shallow tray fermentation 5 days, obtains membranaceous biology cellulose on the top layer of substratum at 30 ℃.Biology cellulose output is 11.8g/L.The Young's modulus of wet biology cellulose film is 6.79Gpa.Gained biology cellulose film is clear, colorless, news have light carambola fragrance.
Embodiment 3:
After the peeling of fresh carambola, squeezing is concentrated, makes 12% the carambola fruit juice of sugar degree at weight percent.
Gather immature papaya slurries, with filtering and make Fructus Chaenomelis extract after tannin precipitator method removal impurity.Described Fructus Chaenomelis extract is added in soybean protein, and water at normal temperature solution 1 day, makes soy peptone, and crushed after being dried makes the soy peptone that molecular weight is 2000 left and right.
Prepare fermention medium by following weight percent consumption:
The carambola fruit juice of sugar degree 12%: 90%;
Soy peptone: 5%;
Regulate pH6.2 with Glacial acetic acid.
Water: surplus.
To the seed liquor of inoculating biology cellulose fermentative production bacterial classification in the above-mentioned fermention medium preparing, as acetobacter xylinum, static shallow tray fermentation 5 days, obtains membranaceous biology cellulose on the top layer of substratum at 30 ℃.Biology cellulose output is 10.9g/L.The Young's modulus of biology cellulose is 7.04Gpa.Gained biology cellulose film is clear, colorless, news have light carambola fragrance.
Embodiment 4:
After the peeling of fresh carambola, squeezing is concentrated, makes 20% the carambola fruit juice of sugar degree at weight percent.
Gather immature papaya slurries, with filtering and make Fructus Chaenomelis extract after tannin precipitator method removal impurity.Described Fructus Chaenomelis extract is added in soybean protein, and water at normal temperature solution 1 day, makes soy peptone, and crushed after being dried makes the soy peptone that molecular weight is 2000 left and right.
Prepare fermention medium by following weight percent consumption:
The carambola fruit juice of sugar degree 20%: 60%;
Soy peptone: 10%;
Regulate pH6.2 with Glacial acetic acid.
Water: surplus.
To the seed liquor of inoculating biology cellulose fermentative production bacterial classification in the above-mentioned fermention medium preparing, as acetobacter xylinum, static shallow tray fermentation 5 days, obtains membranaceous biology cellulose on the top layer of substratum at 30 ℃.Biology cellulose output is 13.8g/L.The Young's modulus of biology cellulose is 7.15Gpa.Gained biology cellulose film is clear, colorless, news have light carambola fragrance.
Illustrate: the seed liquor of the fermented bacterium that above-described embodiment adopts is under equal conditions, the activation of identical bacterial strain obtains.Gained fermentation results data are the mean value of repeatedly parallel test.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. the purposes of carambola fruit juice in biology cellulose fermention medium, is characterized in that: in described fermention medium, containing the carambola fruit juice of weight percent 50 ~ 90%, described carambola fruit juice sugar degree is at the more than 10% of weight percent; After described carambola fruit juice is fresh carambola peeling, the concentrated gained of squeezing; In described fermention medium, also contain vegetable protein peptone, described vegetable protein peptone is in vegetable-protein, to add Fructus Chaenomelis extract hydrolysis gained; Described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained after tannin precipitator method removal impurity.
2. purposes according to claim 1, is characterized in that: described vegetable-protein is soybean protein and/or gluten.
3. purposes according to claim 1, is characterized in that: described vegetable protein peptone be in vegetable-protein, add Fructus Chaenomelis extract to be hydrolyzed and be dried to meal after obtain; Its molecular weight is 2000 left and right.
4. a biology cellulose fermention medium, is characterized in that: the carambola fruit juice that contains weight percent 50 ~ 90%, and described carambola fruit juice sugar degree is at the more than 10% of weight percent, and after described carambola fruit juice is fresh carambola peeling, squeezing concentrates gained; Also contain vegetable-protein and be hydrolyzed the vegetable protein peptone obtaining through Fructus Chaenomelis extract, described Fructus Chaenomelis extract is for gathering immature papaya slurries, with filtering gained after tannin precipitator method removal impurity.
5. fermention medium according to claim 4, is characterized in that: described vegetable-protein is soybean protein and/or gluten.
6. fermention medium according to claim 4, is characterized in that: described vegetable protein peptone be in vegetable-protein, add Fructus Chaenomelis extract to be hydrolyzed and be dried to meal after obtain; Its molecular weight is 2000 left and right.
7. fermention medium according to claim 4, is characterized in that: the pH value of described substratum is 5.2-6.5.
8. fermention medium claimed in claim 4, it is for fermentation to produce biological Mierocrystalline cellulose.
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Citations (3)
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| CN1418562A (en) * | 2002-12-23 | 2003-05-21 | 范铁生 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
| CN102242166A (en) * | 2011-06-28 | 2011-11-16 | 东华大学 | Preparation method of Bacterial Cellulose (BC) with carbon source of inulin |
| CN102250985A (en) * | 2011-08-05 | 2011-11-23 | 海南椰国食品有限公司 | Fermentation medium for bacterial cellulose |
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Patent Citations (3)
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|---|---|---|---|---|
| CN1418562A (en) * | 2002-12-23 | 2003-05-21 | 范铁生 | Method for prodn. of vegetable peptone by enzyme method using soyabean as raw material |
| CN102242166A (en) * | 2011-06-28 | 2011-11-16 | 东华大学 | Preparation method of Bacterial Cellulose (BC) with carbon source of inulin |
| CN102250985A (en) * | 2011-08-05 | 2011-11-23 | 海南椰国食品有限公司 | Fermentation medium for bacterial cellulose |
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