CN1334259A - Process for preparing dual-acid leaf fertilizer by mixing earthworm with bacteria and enzymolysis - Google Patents
Process for preparing dual-acid leaf fertilizer by mixing earthworm with bacteria and enzymolysis Download PDFInfo
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- CN1334259A CN1334259A CN00122132A CN00122132A CN1334259A CN 1334259 A CN1334259 A CN 1334259A CN 00122132 A CN00122132 A CN 00122132A CN 00122132 A CN00122132 A CN 00122132A CN 1334259 A CN1334259 A CN 1334259A
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- Prior art keywords
- enzymolysis
- earthworm
- add
- geotrichum candidum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
A dual-acid leaf fertilizer is prepared from earthworm and reticularia thallus through mincing living earthworm, mixing with reticularia thallus, enzymolyzing, and homogenizing. It contains both amino acids and nucleotide and has high effect on promoting synthesis of protein and nucleic acid for increasing yield and quality of crops.
Description
The present invention relates to a kind of method by earthworm and geotrichum candidum thalline mixed enzymolysis manufacturing bisgallic acid foliage fertilizer, it belongs to the organic fertilizer production technical field.
In recent years, the research and development of amino acid fertilizer are very active, and production application has had very big development.Facts have proved that amino acid fertilizer has significant effect of increasing production to farm crop.The bisgallic acid foliage fertilizer that people such as the Liu Qiang of Agricultural University Of Hunan utilize the waste beer yeast development not only contains amino acid but also contain Nucleotide, and the research of amino acid fertilizer has been pushed to a new level.But the waste beer yeast that this method is utilized must be through broken wall treatment, and degradation technique is cumbersome.
In order to improve the deficiency of prior art, the invention provides a kind of method of utilizing earthworm and geotrichum candidum thalline mixed enzymolysis, make the bisgallic acid foliage fertilizer.
The technology that earthworm bacterium enzymolysis of the present invention is made the bisgallic acid foliage fertilizer is:
Earthworm lives---→ rub-
→ enzymolysis → filtration → concentrate → chelatropic reaction → composite → finished product packing
The geotrichum candidum thalline---
Process for preparing dual-acid leaf fertilizer by mixing earthworm with bacteria and enzymolysis technology of the present invention is specifically described as follows:
1, earthworm pre-treatment: the earthworm alive that will gather washes dirts such as silt, soaks then 1～4 hour, makes its foreign material such as the interior silt of body that spue, and it is cleaned to drain again, adds a little ethanol, rubs into the earthworm slurry with pulverizer.
2, geotrichum candidum yeast culture: nutrient solution is for making wine lees liquor, and sugar degree is adjusted to 0.4～0.5%, adds 0.1% (NH
4)
2SO
4, 0.06%KH
2PO
4PH value is adjusted to 4～4.5, inserts 10% geotrichum candidum seed liquor.Culture condition: 28～32 ℃ of temperature, ventilating ratio are per minute 1: 0.5～1.5, incubation time 9～12 hours.Through Plate Filtration, collect filter cake and promptly get the geotrichum candidum thalline.
3, homogenized: earthworm slurry and geotrichum candidum thalline mix by 1: 1 weight ratio, add 2～8% catalyzer, use the refiner homogenized, get earthworm bacterium mixed pulp.
4, enzyme digestion reaction: earthworm bacterium mixed pulp is added in the enzymolysis reactor, start agitator, be warming up to 40～60 ℃, add 100～200PPm compound protease, after fully stirring evenly, insulation enzymolysis 10～20 hours.Transfer pH value to 7.5 with alkali lye, add 2.5% phosphoric acid=esterase liquid, be incubated 63～65 ℃, degraded 4 hours, so close and be cooled to 50 ℃, add 5.0% phosphoric acid=esterase liquid again, PH keeps 7.5, stirs and be incubated 4 hours, and enzymolysis is finished.More than the preparation method of used phosphoric acid=sour enzyme liquid be: root of Cornu Cervi Pantotrichum is added 4～5 times of water, soaked 20 hours, circulation is soaked and was drenched 6 hours then, filters the back and collects clear liquid and promptly get phosphoric acid=esterase liquid.
5, filter: enzymolysis solution is filtered with flame filter press, collect filtrate.
6, concentrate: filtrate is added in the vacuum concentration pot, and temperature is controlled at 80～120 ℃, and 1/2～1/3 moisture is removed in evaporation.
7, chelatropic reaction: concentrated solution is transferred to adding citric acid accent PH to 4 in the chelatropic reaction device, be warming up to 100～110 ℃, slowly add 1～5% mineral element.Chelatropic reaction 20～30 minutes, cooled and filtered.
8, composite: filtrate adds in the clarifixator, adds alkylphenol polyoxyethylene, the millesimal Sodium Benzoate of total amount 1%, carries out homogenization treatment, measures canned getting product.
Bisgallic acid foliage fertilizer of the present invention is a kind of new and effective foliage spray.It contains kilnitamin more than 10% and 1～3% Nucleotide and various trace elements, and farm crop protein and nucleic acid synthetic had mutual promoting action, is widely used in food crop and cash crop, can significantly improve output, improves quality.
Claims (2)
1, the present invention relates to a kind of method of making the bisgallic acid foliage fertilizer by earthworm and geotrichum candidum thalline mixed enzymolysis, be after the work earthworm is rubbed the back and the geotrichum candidum thalline mixes, carry out enzyme digestion reaction, concentrate after filtering, carry out chelatropic reaction, composite homogeneous again and promptly get the bisgallic acid foliage fertilizer that not only contains amino acid but also contain Nucleotide.
2, earthworm as claimed in claim 1 and geotrichum candidum thalline mixed enzymolysis are made the method for bisgallic acid foliage fertilizer, it is characterized in that enzymolysis process is: earthworm is rubbed the back mix by 1: 1 weight ratio with the geotrichum candidum thalline, add 2～8% catalyzer, after homogenized, add enzymolysis reactor, add 100～200PPm compound protease, insulation enzymolysis 10～20 hours, transfer PH to 7.5 with alkali lye, add 2.5% phosphoric acid=esterase liquid, be incubated 63～65 ℃, degraded 4 hours, be cooled to 50 ℃ then, add 5.0% phosphodiesterase liquid again, pH value keeps 7.5, and the insulation enzymolysis was promptly finished enzymolysis in 4 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00122132A CN1334259A (en) | 2000-07-19 | 2000-07-19 | Process for preparing dual-acid leaf fertilizer by mixing earthworm with bacteria and enzymolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00122132A CN1334259A (en) | 2000-07-19 | 2000-07-19 | Process for preparing dual-acid leaf fertilizer by mixing earthworm with bacteria and enzymolysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1334259A true CN1334259A (en) | 2002-02-06 |
Family
ID=4589117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00122132A Pending CN1334259A (en) | 2000-07-19 | 2000-07-19 | Process for preparing dual-acid leaf fertilizer by mixing earthworm with bacteria and enzymolysis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1334259A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731211A (en) * | 2012-07-09 | 2012-10-17 | 大连珍奥生物农业科技股份有限公司 | Nucleotide-containing fertilizer |
| CN103204733A (en) * | 2010-10-21 | 2013-07-17 | 高旌 | High-quality high yield production method of rhizomatic traditional Chinese medical materials |
| CN103755396A (en) * | 2013-12-17 | 2014-04-30 | 扬州大学 | Preparation method of earthworm composite liquid for promoting wheat yield increase |
| CN104303666A (en) * | 2014-10-14 | 2015-01-28 | 扬州大学 | Method for increasing yield of okra |
| CN105272387A (en) * | 2015-10-30 | 2016-01-27 | 郭新荣 | Method for producing earthworm free amino acid active enzyme foliar fertilizer in autogenesis-enzyme way |
| CN105399532A (en) * | 2015-12-21 | 2016-03-16 | 上海永通化工有限公司 | Water-soluble amino acid fertilizer and preparation method thereof |
-
2000
- 2000-07-19 CN CN00122132A patent/CN1334259A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103204733A (en) * | 2010-10-21 | 2013-07-17 | 高旌 | High-quality high yield production method of rhizomatic traditional Chinese medical materials |
| CN103204733B (en) * | 2010-10-21 | 2014-12-17 | 亳州师范高等专科学校 | Production method of rhizomatic traditional Chinese medical materials |
| CN102731211A (en) * | 2012-07-09 | 2012-10-17 | 大连珍奥生物农业科技股份有限公司 | Nucleotide-containing fertilizer |
| CN103755396A (en) * | 2013-12-17 | 2014-04-30 | 扬州大学 | Preparation method of earthworm composite liquid for promoting wheat yield increase |
| CN103755396B (en) * | 2013-12-17 | 2016-08-17 | 扬州大学 | A kind of preparation method of the Lumbricus complex liquid promoting wheat increase yield |
| CN104303666A (en) * | 2014-10-14 | 2015-01-28 | 扬州大学 | Method for increasing yield of okra |
| CN105272387A (en) * | 2015-10-30 | 2016-01-27 | 郭新荣 | Method for producing earthworm free amino acid active enzyme foliar fertilizer in autogenesis-enzyme way |
| CN105399532A (en) * | 2015-12-21 | 2016-03-16 | 上海永通化工有限公司 | Water-soluble amino acid fertilizer and preparation method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |