CN1418550A - Products from fungi fermentation used for inducing disease resistance of plants - Google Patents
Products from fungi fermentation used for inducing disease resistance of plants Download PDFInfo
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- CN1418550A CN1418550A CN 02155325 CN02155325A CN1418550A CN 1418550 A CN1418550 A CN 1418550A CN 02155325 CN02155325 CN 02155325 CN 02155325 A CN02155325 A CN 02155325A CN 1418550 A CN1418550 A CN 1418550A
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Abstract
The present invention relates to a fermentation condition of fermented product of Agaricus blazei and tests the effect of said fermented product for resisting plant disease. Its fermentation condition includes culture medium formula and its temp. and fermentation period and rotating speed of shaking table. Its disease-resisting effect includes the disease-resisting effect of different fermentation time and different dilution degree of fermented product. The dosage form of the the prepared fermented product is wate preparation, and the tween is used as penetrating agent, and can be sprayed on cucumber seedling in growth stage, and can induce and activate self-defence system for resisting disease so as to attain the goal of preventing and curing diseases. It can be used for curing scab of cucumber.
Description
Fungi Agaricus blazei Murrill (Agaricus blazei) tunning is a kind of low toxin preparation as plant disease resistance inductor, and its disease-resistant principle is to induce the also disease-resistant system of defense of activated plant self.Direct purpose of the present invention provides the disease-resistant biopesticide of safety, low toxicity.
Fungal bacterial strain Agaricus blazei Murrill ACCC50654, Chinese agriculture microorganism fungus kind preservation center provides.Tunning obtains through following method: hypha,hyphae is cultivated through flat board, be inoculated in the liquid fermentation medium, and 25 ℃ of constant temperature, shaking speed 150rpm, the cycle is 7 days.Fermentative medium formula is a corn starch 3.50%, dusty yeast 0.30%, KH
2PO
40.20%, MgSO
40.15%, CaCl
20.05%, the 0.20ml/100ml corn steep liquor.Fermentation ends is with the mycelia filtered and recycled, and tunning is present in the zymotic fluid and can directly uses as plant disease resistance inductor.Active ingredient is fungi polysaccharide and oligosaccharides.Said preparation uses formulation to be aqua, need add 2/10000ths tween during use as bleeding agent.Long preservation adds millesimal Sodium Benzoate.
Embodiment:
1. the tunning scab resistant effect of different fermentations fate.
With the fungal fermented filtrate of different fermentations fate, be aided with the bleeding agent tween, treat that the seed of Fructus Cucumidis sativi shell comes off, during the cotyledon yellow, spray disease-resistant derivant three times, induce to be spaced apart 1 day at every turn.With the etiolated seedling that sprays clear water in contrast.In same time inoculation dosporium cucumerinumand its, when spraying the contrast etiolated seedling morbidity of clear water, begin the classification investigation.Preventive effect preceding 7 days for increasing with fate, the 7th day and the 8th day effect are more or less the same, and descend since the 9th day.The highest total preventive effect is 74.80%.Bioassay results sees attached list 1.
2. the 7th of different diluted concentrations the day anti-scab of cucumber effect of tunning.
The fungal fermented filtrate of fermentation in the 7th day is diluted 2 times respectively, 4 times and 8 times, be aided with the bleeding agent tween, treat that the seed of Fructus Cucumidis sativi shell comes off, during the cotyledon yellow, spray disease-resistant derivant three times, induce to be spaced apart 1 day at every turn.With the etiolated seedling that sprays clear water in contrast.In same time inoculation dosporium cucumerinumand its, when spraying the contrast etiolated seedling morbidity of clear water, begin the classification investigation.Preventive effect is successively decreased with extension rate.Bioassay results 2 specifications of seeing attached list
Subordinate list 1 bioassay results
Fermentation time (my god) leaf disease refer to (%) leaf preventive effect (%) handle disease refer to (%) handle preventive effect (%) zymotic fluid 0----
3 44.97 31.90 69.07 6.65
4 43?86 33.59 73.33 0.89
5 27.85 57.83 55.07 25.57
6 19.88 69.90 46.51 37?14
7 12?63 80.88 23.15 68.71
8 8.35 87.36 38.49 47.98
9 17.18 73?99 36?36 50.86
10 21.62 67.26 48.82 34.02
11 22.10 66.54 46.70 36.36 contrast (water) 66 04-73.99-mycelia polysaccharide solution, 22.07 66.58 24.27 67.20 fruitbody polysaccharide solution 6.93 89.51 10.89 85.28
Subordinate list 2 dilution test measurement results
The sample leaf disease refers to that (%) leaf preventive effect (%) handle disease refers to that (%) handle preventive effect (%) rare 1 12.63 80.88 23.15 68.71 releases 2 39.17 40.69 48.67 34.22 times of 4 49.38 25.23 58.33 21.16 several 8 54.17 17.97 63.08 14.74 contrast (water) 66.04-73.99-bacterial classifications explanation strain name: Ji Songrong (Agaricus blazei); China Committee for Culture Collection of Microorganisms is common little
Register on the books and be numbered in biological center: CGMSCC NO.0567
Claims (6)
1 the present invention relates to a kind of fungus fermentation products is applied to anti-plant disease, and content comprises the preparation of fungus fermentation products and uses the using method of this tunning inducing plant opposing plant disease.
The preparation method of the described fungus fermentation products of 2 the present invention claims 1 comprises: strain name, mycelia solid culture, mycelia liquid fermentation condition, tunning directly spray.Tunning is a liquor, and main component is fungi polysaccharide and oligosaccharides, and they have induces the also function of the disease-resistant system of defense of activated plant self.
The using method of the anti-plant disease of the described fungus fermentation products of 3 the present invention claims 1 comprises: the tunning that obtains with the described methods of the present invention 2 all has the effect of inducing anti-disease from the 3rd day of fermentation to fermentation ends, reaches maximum at the 7th day.With 2 times, 4 times, 8 times effects that inducing anti-disease is all arranged of tunning dilution, but effect obviously descends, and the inducing anti-disease effect of former tunning is the most obvious.
The described bleeding agent of 4 the present invention claims 1 is a polysorbas20, and using dosage is 2/10000ths.
5 fungus fermentation products by the preparation of the present invention claim 2, and by the described method use of the present invention claim 3, anti-phytopathogen black star germ.
6 this invention claims 2,3,4,5 integrated applications.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02155325 CN1255034C (en) | 2002-12-10 | 2002-12-10 | Products from fungi fermentation used for inducing disease resistance of plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02155325 CN1255034C (en) | 2002-12-10 | 2002-12-10 | Products from fungi fermentation used for inducing disease resistance of plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1418550A true CN1418550A (en) | 2003-05-21 |
| CN1255034C CN1255034C (en) | 2006-05-10 |
Family
ID=4752601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02155325 Expired - Fee Related CN1255034C (en) | 2002-12-10 | 2002-12-10 | Products from fungi fermentation used for inducing disease resistance of plants |
Country Status (1)
| Country | Link |
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| CN (1) | CN1255034C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110036855A (en) * | 2019-05-23 | 2019-07-23 | 申琳 | A method of regulation Sweet Potato For High. yield Production with it is high-quality unified |
| CN112931024A (en) * | 2021-01-25 | 2021-06-11 | 王文明 | Cultivation method for reducing grape diseases |
-
2002
- 2002-12-10 CN CN 02155325 patent/CN1255034C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110036855A (en) * | 2019-05-23 | 2019-07-23 | 申琳 | A method of regulation Sweet Potato For High. yield Production with it is high-quality unified |
| CN110036855B (en) * | 2019-05-23 | 2022-02-01 | 申琳 | Method for regulating high yield and high quality of sweet potatoes |
| CN112931024A (en) * | 2021-01-25 | 2021-06-11 | 王文明 | Cultivation method for reducing grape diseases |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1255034C (en) | 2006-05-10 |
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