CN101649295A - Bacterial strain MT-06 and application thereof - Google Patents
Bacterial strain MT-06 and application thereof Download PDFInfo
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- CN101649295A CN101649295A CN200910152681A CN200910152681A CN101649295A CN 101649295 A CN101649295 A CN 101649295A CN 200910152681 A CN200910152681 A CN 200910152681A CN 200910152681 A CN200910152681 A CN 200910152681A CN 101649295 A CN101649295 A CN 101649295A
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Abstract
The invention discloses a bacterial strain MT-06 which is a talaromyces flavus MT-06 CGMCCNo:2865, and simultaneously discloses a method for preventing and treating powdery mildew of strawberry by thebacterial strain MT-06 of the invention. The invention aims to a biological-proof fungi of strawberry diseases, antagonists MT-06 has good prevention effect to the powdery mildew of strawberry, in particular has excellent effects on the aspect of the persistence, the invention is safe to the environment, people, livestock and beneficial organisms.
Description
Technical field
The present invention relates to champac shape bacterium (Talaromyces flavus) MT-06 bacterial strain and related application thereof, specifically, relate to contain will have suppress that Plant diseases takes place and champac shape bacterium (Talaromycesflavus) the MT-06 bacterial strain of preventive effect as plant disease control agent, and use the method for control of above-mentioned agent for preventing and eliminating.
Background technology
Powdery mildew of strawberry, as a kind of Plant diseases, the method of control for example has, and utilizes crop rotation, solar heat to cultivate and carry out physics and prevent and kill off, utilize chemical pesticide to carry out chemical control, use disease-resistant variety and prevent and kill off, utilize the microorganism that the hypotoxicity pathogenic bacteria is had an antagonistic action to carry out biocontrol.In the middle of this, the development research development in recent years of chemical pesticide, particularly organic synthesis sterilant is swift and violent unusually, developed in frequent succession have higher effectiveness, the various medicaments of same-action not, also further developed multiple application process simultaneously.The chemical control method of using above-mentioned medicament is prevented and kill off and is prevented and kill off powdery mildew of strawberry and saves the labor force in the operation and made very big contribution, is widely used.But in recent years, because the low phenomenon of chemical control method preventive effect has appearred in the generation of resistant organism.In addition, because the strawberry plant is short, disease and pest takes place in fruit delicacy easily, and especially in collection period, powdery mildew of strawberry, gray mold etc. take place very serious.For increasing both production and income, some orchard workers even between collection period, also repeatedly spray height and poison the medicine of learning to farm, and the strawberry surface irregularity is easy to remains of pesticide.Therefore, often occur the problem of strawberry fruit agricultural chemicals severe overweight in recent years, had a strong impact on the development of strawberry industry.In addition, repeatedly use chemical pesticide repeatedly in a large number, the non-existent chemical substance of nature is discharged in the environment, much less animals and plants are had direct toxic medicament, even the baneful influence that the incomplete medicament that discharges causes environment also is alarming.
In view of above-mentioned, carrying out disease with chemical pesticide prevents and kill off, because the appearance of resistant organism makes the low possibility of preventive effect higher, simultaneously chemical pesticide problem such as residual and pollution on the environment on strawberry fruit is serious, in this case, necessitate at the new biological bactericide of the main disease research and development of strawberry.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, provide bacterial strain MT-06 and the control of powdery mildew of strawberry.
A kind of bacterial strain MT-06 of the present invention is characterized in that: this bacterial strain is champac shape bacterium MT-06 CGMCCNo:2865.Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The application of bacterial strain MT-06 of the present invention, bacterial strain MT-06 are to the method for preventing and treating of powdery mildew of strawberry:
(1) making of bacterial strain MT-06 bacterium liquid: earlier with bacterial strain MT-06 on the PDA substratum, cultivate after 5～10 days for 25 ℃～30 ℃, the MT-06 bacterial strain diameter of PDA culture medium culturing on the ware of making even is about 5 of the circular fritters of 4mm～6mm and puts into the PDA liquid nutrient medium of having sterilized, make 3 bottles, 28 ℃ leave standstill cultivation; After 12～15 days,, after stirrer grinds, get mixed solution with conidium concentration dilution to 10 with above-mentioned nutrient solution
5～10
6Stand-by behind individual/ml;
(2) at the powdery mildew of strawberry their early stage, with the mixed solution dispenser after the dilution, dispenser is three times altogether.
The mode of dispenser is a spray pattern in the step (2).
Dispenser three times is respectively in the dispenser for the first time of powdery mildew of strawberry their early stage in the step (2), after the dispenser for the first time 7d, 14d carry out for the second time, dispenser for the third time.
The present invention has following beneficial effect:
The present invention is directed to a kind of biocontrol microorganisms of strawberry disease, antagonism bacterium MT-06 has preventive effect preferably to powdery mildew of strawberry, especially aspect long-lasting, have outstanding performance, and to environment, people and animals and beneficial organism safety.Carried out the research of the Biocontrol Mechanism aspect of this bacterial strain simultaneously, laid a good foundation for further this bacterium being developed and utilized as the biological and ecological methods to prevent plant disease, pests, and erosion factor.
Embodiment
Below in conjunction with embodiment technical scheme of the present invention is described:
A kind of bacterial strain MT-06, this bacterial strain are champac shape bacterium MT-06CGMCC No:2865.
One, the grown form of bacterial strain MT-06
Be isometrical radiation growth on the PDA substratum, the bacterium colony circle is the carpet-like tiling, initial stage is a lemon yellow, and after 25 ℃ of two weeks of cultivation, the bacterium colony surface forms the perithecium of yellow little point-like, be spherical, orange red after the perithecium maturation, size is 237～352 μ m, forms ascus in it; Ascus is long bar-shaped, and length 8.1～9.6 μ m include 8 thecaspores; The thecaspore circle, size 3.9～4.8 μ m * 3.2～3.6 μ m.
Two, the biological characteristics of bacterial strain MT-06
The different substratum of table 1 are to the influence of mycelial growth and product spore
Bacterial strain speed of growth on PSA and PDA is the fastest, secondly is bean sprouts medium, medium oatmeal and yeast extract paste glucose agar medium, poor growth on Starch Agar and Cha Shi substratum.
It is maximum that the PDA substratum produces spore, be PSA, bean sprouts medium, yeast extract paste glucose agar medium secondly, and Cha Shi substratum, medium oatmeal, that the Starch Agar substratum produces spore is relatively poor, should not be used for doing product spore substratum.
Table 2 different illumination conditions is to the influence of mycelial growth and product spore
Light application time is long more, and the speed of growth of bacterial strain is fast more, but illumination is not very big to the influence of strain growth speed.
Complete black environment helped producing spore in 24 hours, and sporulation quantity is significantly higher than other processing, and other four kinds of processing all are unfavorable for producing spore.
Table 3 different pH value mycelial growth and the influence of producing spore
Bacterial strain all can be grown in the scope of pH4～12, and optimal pH is 4～7, and growth better under neutral meta-acid condition.
Bacterial strain all can produce spore in pH4～12.PH is 4～9 o'clock sporulation quantities to be increased with the rising of pH, is that 9 o'clock sporulation quantities are the highest at pH, and pH is that＞9 o'clock sporulation quantities descend rapidly with the rising of pH.
Table 4 differing temps is to the influence of mycelial growth and product spore
The colony growth optimum temperuture is 30 ℃, is lower than 10 ℃ or be higher than 40 ℃ and be unfavorable for its growth.Bacterial strain all can produce spore at 25～35 ℃, and the suitableeest product spore temperature is 30 ℃.
Embodiment one bacterial strain MT-06 is to the prevention effect of powdery mildew of strawberry
The making of bacterial strain MT-06 bacterium liquid: earlier with bacterial strain MT-06 on the PDA substratum, cultivate after 5～10 days for 25 ℃～30 ℃, MT-06 bacterial strain (band substratum) diameter of PDA culture medium culturing on the ware of making even is about 5 of the circular fritters of 4mm～6mm and puts into the PDA liquid nutrient medium of having sterilized, make 3 bottles, 28 ℃ leave standstill cultivation; After 12～15 days, with above-mentioned nutrient solution, after stirrer grinds, obtain mixed solution, mixed solution is centrifugal, gets supernatant liquor, culture (mycelia and spore), and supernatant liquor and culture respectively dilute 10 times, obtain spore concentration 10
5～10
6Individual/ml, stand-by.
Dispenser: 5 processing are established in this test altogether, and 3 repetitions amount to 15 sub-districts, every sub-district strawberry area 1m
2(kind: Feng Xiang), adopt district's group arrangement at random.5 processing are respectively the MT-06 strain liquid and cultivate mixed solution, MT-06 strain cultured solution (nutrient solutions after 10 times of dilutions of supernatant liquor), and MT-06 strain culture (gruel 10 times after mycelia and spore), emerald green shellfish 50% dried suspension, clear water is treated to blank.
In the dispenser for the first time of powdery mildew of strawberry their early stage, 7d, 14d carry out the second time, dispenser for the third time after the dispenser for the first time.Adopt Jacto-HD400 type hand sprayer, the complete stool even spraying, dispenser is three times altogether.
State of an illness investigation
The eve of dispenser for the first time investigation state of an illness radix, 7d, 14d after the dispenser for the third time.10 samplings in every sub-district are selected 3 representative compound leaves of every strain, the percentage classification that accounts for whole leaflet area with lesion area on each leaflet on the compound leaf.According to the sick leaf disease level of following stage division record different treatment, calculate disease index and relative prevention effect, The data Turkey method is carried out statistical study [4].
Stage division:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-15% of whole leaf area;
5 grades: lesion area accounts for the 16%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Disease index (%)=[∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * the highest sick progression)] * 100
Prevention effect (%)=the 1-[(CK0 disease refers to * the pt1 disease refers to)/(CK1 disease refer to * pt0 disease refer to)] * 100
Wherein: CK0 represents the preceding disease index of blank district dispenser; CK1 represents disease index after the dispenser of blank district; Pt0 represents the preceding disease index of chemicals treatment district dispenser; Pt1 represents disease index after the dispenser of chemicals treatment district.
Result and analysis
Each processing of table 5 is to the prevention effect analysis of powdery mildew of strawberry.
Test-results shows, finishes three dispensers after 7 days, and three kinds of processing of strains tested all have the better prevention effect to disease, and wherein the preventive effect of mixed solution is best, is only second to the preventive effect of the emerald green shellfish 50% dried suspension of sterilant, and culture takes second place, and is nutrient solution once more.Finish three dispensers after 14 days, strains tested mixed solution and culture obviously rise to the prevention effect of disease, and have surpassed the emerald green shellfish 50% dried suspension of sterilant.As time goes on the preventive effect of emerald green shellfish 50% dried suspension and strain cultured solution has then reduced.
At last, it is also to be noted that what more than enumerate only is specific embodiments of the invention.Obviously, the invention is not restricted to above examples of implementation, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (4)
1, a kind of bacterial strain MT-06 is characterized in that: this bacterial strain is champac shape bacterium MT-06CGMCC No:2865.
2, the application of the described bacterial strain MT-06 of claim 1 is characterized in that the prevent and treat method of bacterial strain MT-06 to powdery mildew of strawberry:
(1) making of bacterial strain MT-06 bacterium liquid: with bacterial strain MT-06 on the PDA substratum, cultivate after 5～10 days for 25 ℃～30 ℃, the MT-06 bacterial strain diameter of PDA culture medium culturing on the ware of making even is about 5 of the circular fritters of 4mm～6mm and puts into the PDA liquid nutrient medium of having sterilized, make 3 bottles, 28 ℃ leave standstill cultivation; After 12～15 days,, after stirrer grinds, get mixed solution with conidium concentration dilution to 10 with above-mentioned nutrient solution
5～10
6Stand-by behind individual/ml;
(2) at the powdery mildew of strawberry their early stage, with the mixed solution dispenser after the dilution, dispenser is three times altogether.
3, the application of bacterial strain MT-06 according to claim 2 is characterized in that: the mode of dispenser is a spray pattern in the step (2).
4, the application of bacterial strain MT-06 according to claim 2 is characterized in that: in the step (2) dispenser three times respectively in the dispenser for the first time of powdery mildew of strawberry their early stage, after the dispenser for the first time 7d, 14d carry out for the second time, dispenser for the third time.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910152681A CN101649295A (en) | 2009-09-17 | 2009-09-17 | Bacterial strain MT-06 and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910152681A CN101649295A (en) | 2009-09-17 | 2009-09-17 | Bacterial strain MT-06 and application thereof |
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| CN101649295A true CN101649295A (en) | 2010-02-17 |
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ID=41671623
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286383A (en) * | 2011-09-06 | 2011-12-21 | 青岛农业大学 | Talaromyces flavus and application of same in prevention of plant pathogens |
| CN107176781A (en) * | 2017-05-22 | 2017-09-19 | 江南大学 | A kind of method that utilization champac shape fungal mycelium improves dewatering performance of sludge |
| CN109593658A (en) * | 2018-12-27 | 2019-04-09 | 河南科技大学 | A kind of endogenetic fungus ZXL-SZY-R-9 and its application with antibiosis |
-
2009
- 2009-09-17 CN CN200910152681A patent/CN101649295A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286383A (en) * | 2011-09-06 | 2011-12-21 | 青岛农业大学 | Talaromyces flavus and application of same in prevention of plant pathogens |
| CN102286383B (en) * | 2011-09-06 | 2013-05-29 | 青岛农业大学 | Talaromyces flavus and application of same in prevention of plant pathogens |
| CN107176781A (en) * | 2017-05-22 | 2017-09-19 | 江南大学 | A kind of method that utilization champac shape fungal mycelium improves dewatering performance of sludge |
| CN107176781B (en) * | 2017-05-22 | 2019-08-16 | 江南大学 | A kind of Tarlaromyces flavus, its mycelial preparation method and its improving the application in dewatering performance of sludge and environmental area |
| CN109593658A (en) * | 2018-12-27 | 2019-04-09 | 河南科技大学 | A kind of endogenetic fungus ZXL-SZY-R-9 and its application with antibiosis |
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Open date: 20100217 |