CN1255034C - Products from fungi fermentation used for inducing disease resistance of plants - Google Patents
Products from fungi fermentation used for inducing disease resistance of plants Download PDFInfo
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- CN1255034C CN1255034C CN 02155325 CN02155325A CN1255034C CN 1255034 C CN1255034 C CN 1255034C CN 02155325 CN02155325 CN 02155325 CN 02155325 A CN02155325 A CN 02155325A CN 1255034 C CN1255034 C CN 1255034C
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- cucumber
- fermentation
- opposing
- scab
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Abstract
The present invention relates to a fermentative condition for a fungus agaricus blazei fermentation product, and the plant disease resistance effect of the fermentation product is tested. The fermentation condition comprises a culture medium formulation, a culture medium temperature, a fermentation period, and the rotation speed of a rocking bed. The disease resistance effect comprises the disease resistance effects of different fermentation time and different product dilution. The used formulation of the prepared fermentation product is an aqueous agent which is added with tween to be used as a penetrating agent; the penetrating agent is sprayed on cucumber seedlings during a growth period to induce and activate the disease resistant defense system of plants, so that the aim of preventing and curing diseases are achieved. The present invention is used for resisting scab of cucumber.
Description
Fungi Agaricus blazei Murrill (Agaricus blazei) tunning is a kind of low toxin preparation as plant disease resistance inductor, and its disease-resistant principle is to induce the also disease-resistant system of defense of activated plant self.Direct purpose of the present invention provides the disease-resistant biopesticide of safety, low toxicity.
Fungal bacterial strain Agaricus blazei Murrill ACCC50654, Chinese agriculture microorganism fungus kind preservation center provides.Tunning obtains through following method: hypha,hyphae is cultivated through flat board, be inoculated in the liquid fermentation medium, and 25 ℃ of constant temperature, shaking speed 150rpm, the cycle is 7 days.Fermentative medium formula is a corn starch 3.50%, dusty yeast 0.30%, KH
2PO
40.20%, MgSO
40.15%, CaCl
20.05%, the 0.20ml/100ml corn steep liquor.Fermentation ends is with the mycelia filtered and recycled, and tunning is present in the zymotic fluid and can directly uses as plant disease resistance inductor.Active ingredient is fungi polysaccharide and oligosaccharides.Said preparation uses formulation to be aqua, need add 2/10000ths tween during use as bleeding agent.Long preservation adds millesimal Sodium Benzoate.
Embodiment:
1. the tunning scab resistant effect of different fermentations fate.
With the fungal fermented filtrate of different fermentations fate, be aided with the bleeding agent tween, treat that the seed of Fructus Cucumidis sativi shell comes off, during the cotyledon yellow, spray disease-resistant derivant three times, induce to be spaced apart 1 day at every turn.With the etiolated seedling that sprays clear water in contrast.In same time inoculation dosporium cucumerinumand its, when spraying the contrast etiolated seedling morbidity of clear water, begin the classification investigation.Preventive effect preceding 7 days for increasing with fate, the 7th day and the 8th day effect are more or less the same, and descend since the 9th day.The highest total preventive effect is 74.80%.Bioassay results is seen
Subordinate list 1.
2. the 7th of different diluted concentrations the day anti-scab of cucumber effect of tunning.
The fungal fermented filtrate of fermentation in the 7th day is diluted 2 times respectively, 4 times and 8 times, be aided with the bleeding agent tween, treat that the seed of Fructus Cucumidis sativi shell comes off, during the cotyledon yellow, spray disease-resistant derivant three times, induce to be spaced apart 1 day at every turn.With the etiolated seedling that sprays clear water in contrast.In same time inoculation dosporium cucumerinumand its, when spraying the contrast etiolated seedling morbidity of clear water, begin the classification investigation.Preventive effect is successively decreased with extension rate.Bioassay results sees attached list 2.
Subordinate list 1 bioassay results
Subordinate list 2 dilution test measurement results
| Fermentation time (my god) | Leaf disease refers to (%) | Leaf preventive effect (%) | The handle disease refers to (%) | Handle preventive effect (%) | |
| Zymotic fluid contrast (water) mycelia polysaccharide solution fruitbody polysaccharide solution | 0 3 4 5 6 7 8 9 10 11 | - 44.97 43.86 27.85 19.88 12.63 8.35 17.18 21.62 22.10 66.04 22.07 6.93 | - 31.90 33.59 57.83 69.90 80.88 87.36 73.99 67.26 66.54 - 66.58 89.51 | - 69.07 73.33 55.07 46.51 23.15 38.49 36.36 48.82 46.70 73.99 24.27 10.89 | - 6.65 0.89 25.57 37.14 68.71 47.98 50.86 34.02 36.36 - 67.20 85.28 |
| Sample | Leaf disease refers to (%) | Leaf preventive effect (%) | The handle disease refers to (%) | Handle preventive effect (%) |
| Rare 1 releases 2 times of 4 several 8 contrast (water) | 12.63 39.17 49.38 54.17 66.04 | 80.88 40.69 25.23 17.97 - | 23.15 48.67 58.33 63.08 73.99 | 68.71 34.22 21.16 14.74 - |
The bacterial classification explanation
Strain name: Agaricus blazei Murrill (Agaricus blazei); China Committee for Culture Collection of Microorganisms registers on the books at the common micro-organisms center and is numbered: CGMSCC NO.0567
Claims (13)
1. the Agaricus blazei Murrill tunning is used for the purposes of inducing plant opposing fungal disease, wherein said tunning preparation method is: it is that the Agaricus blazei Murrill mycelium inoculation of Agaricus blazei CGMSCC 0567 is: corn starch 3.5% in prescription that the cultured Chinese bacterium of planar surface is protected the center registration number, dusty yeast 0.3%, KH
2PO
40.2%, MgSO
40.15%, CaCl
20.05%, the liquid nutrient medium of corn steep liquor 0.2ml/100ml under the condition of 25 ℃ of constant temperature, shaking speed 150rpm, carries out liquid fermentation to obtain tunning.
2. the purposes of claim 1, wherein said Agaricus blazei Murrill bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and registration number is Agaricus blazei CGMSCC 0567.
3. the purposes of claim 1, use therein is after the fermentation ends mycelia to be filtered the fermentation filtered fluid of removing.
4. the purposes of claim 1 wherein is from fermenting the 3rd day to the 11st day, with fermenation raw liquid with dilute 2,4 or 8 times fermentation dilution respectively, sprays in the surface of cucumber etiolated seedling.
5. each purposes among the claim 1-4, use therein is the aqua of tunning.
6. the purposes of claim 5 is added bleeding agent in the wherein said aqua.
7. the purposes of claim 6, wherein the bleeding agent of Tian Jiaing is a polysorbas20, using dosage is 2/10000ths.
8. each purposes among the claim 1-4, wherein inducing cucumber etiolated seedling opposing scab of cucumber.
9. the purposes of claim 5, wherein inducing cucumber etiolated seedling opposing scab of cucumber.
10. the purposes of claim 6, wherein inducing cucumber etiolated seedling opposing scab of cucumber.
11. the purposes of claim 7, wherein inducing cucumber etiolated seedling opposing scab of cucumber.
12. each purposes among the claim 1-4 wherein adopts fungi polysaccharide and oligosaccharides opposing fungal disease in the tunning.
13. the purposes of claim 12, wherein inducing cucumber etiolated seedling opposing scab of cucumber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02155325 CN1255034C (en) | 2002-12-10 | 2002-12-10 | Products from fungi fermentation used for inducing disease resistance of plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02155325 CN1255034C (en) | 2002-12-10 | 2002-12-10 | Products from fungi fermentation used for inducing disease resistance of plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1418550A CN1418550A (en) | 2003-05-21 |
| CN1255034C true CN1255034C (en) | 2006-05-10 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02155325 Expired - Fee Related CN1255034C (en) | 2002-12-10 | 2002-12-10 | Products from fungi fermentation used for inducing disease resistance of plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1255034C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110036855B (en) * | 2019-05-23 | 2022-02-01 | 申琳 | Method for regulating high yield and high quality of sweet potatoes |
| CN112931024A (en) * | 2021-01-25 | 2021-06-11 | 王文明 | Cultivation method for reducing grape diseases |
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2002
- 2002-12-10 CN CN 02155325 patent/CN1255034C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1418550A (en) | 2003-05-21 |
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