CN1418251A - 人干扰素α的表达和分泌载体及通过使用这种载体产生人干扰素α的方法 - Google Patents
人干扰素α的表达和分泌载体及通过使用这种载体产生人干扰素α的方法 Download PDFInfo
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- CN1418251A CN1418251A CN01803860A CN01803860A CN1418251A CN 1418251 A CN1418251 A CN 1418251A CN 01803860 A CN01803860 A CN 01803860A CN 01803860 A CN01803860 A CN 01803860A CN 1418251 A CN1418251 A CN 1418251A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
转化体 | 实施例 | 表达载体 | STII中修饰的氨基酸残基 | 周质中水平* |
HM10600 | 对比实施例1 | pT14SIα-2a | 82±40 | |
HM10601 | 对比实施例1 | pT14SSIα-2a | 325±75 | |
HM10701 | 对比实施例2 | pT14SSIα-2b | 288±90 | |
HM10602 | 实施例1(1) | pT14SSIα-2a-4T | Thr4 | 550±120 |
HM10603 | 实施例1(2) | pT14OSSIα-2a-4T | Thr4 | 1020±135 |
HM10604 | 实施例1(2) | pT14SSIα-2a-4T22Q | Thr4,Gln22 | 680±105 |
HM10611 | 实施例1(2) | pT14OSSIα-2a-4T22Q | Thr4,Gln22 | 1220±120 |
HM10612 | 实施例1(3) | pT14OSSIα-2a-4T20V22Q | Thr4,Val20,Gln22 | 1130±180 |
HM10613 | 实施例2 | pT14NSSIα-2a-4T22Q | Thr4,Gln22 | 750±144 |
HM10614 | 实施例2 | pT14MSSIα-2a-4T22Q | Thr4,Gln22 | 420±100 |
HM10702 | 实施例1(1) | pT14SSIα-2b-4T | Thr4 | 370±90 |
HM10703 | 实施例1(2) | pT14OSSIα-2b-4T | Thr4 | 735±117 |
HM10711 | 实施例1(2) | pT14OSSIα-2b-4T22Q | Thr4,Gln22 | 1070±150 |
HM10712 | 实施例1(3) | pT14OSSIα-2b-4T20V22Q | Thr4,Val20,Gln22 | 820±160 |
*IFNα-2mg/100 O.D600nm/L培养溶液 |
Claims (18)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20002434 | 2000-01-19 | ||
KR2000-2434 | 2000-01-19 | ||
KR1020000002434A KR100360594B1 (ko) | 2000-01-19 | 2000-01-19 | 인간 인터페론 알파의 발현 분비벡터 및 이를 이용한인터페론 알파의 생산 방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1418251A true CN1418251A (zh) | 2003-05-14 |
CN100436581C CN100436581C (zh) | 2008-11-26 |
Family
ID=19639684
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018038603A Expired - Fee Related CN100436581C (zh) | 2000-01-19 | 2001-01-19 | 人干扰素α的表达和分泌载体及通过使用这种载体产生人干扰素α的方法 |
Country Status (14)
Country | Link |
---|---|
US (1) | US7052867B2 (zh) |
EP (1) | EP1250439B1 (zh) |
JP (1) | JP3751883B2 (zh) |
KR (1) | KR100360594B1 (zh) |
CN (1) | CN100436581C (zh) |
AT (1) | ATE371027T1 (zh) |
AU (1) | AU762053B2 (zh) |
BR (1) | BRPI0107708A8 (zh) |
CA (1) | CA2397527C (zh) |
DE (1) | DE60130100T2 (zh) |
ES (1) | ES2290156T3 (zh) |
NZ (1) | NZ520076A (zh) |
RU (1) | RU2230116C2 (zh) |
WO (1) | WO2001057217A1 (zh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1656455B1 (en) * | 2003-08-13 | 2012-09-19 | Sandoz Ag | Process for the purification of recombinant polypeptides |
WO2005017162A2 (en) * | 2003-08-13 | 2005-02-24 | Sandoz Ag | Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides |
GB0319601D0 (en) * | 2003-08-20 | 2003-09-24 | Sandoz Ag | Production process |
US8110665B2 (en) | 2003-11-13 | 2012-02-07 | Hanmi Holdings Co., Ltd. | Pharmaceutical composition comprising an immunoglobulin FC region as a carrier |
EP1682584B1 (en) | 2003-11-13 | 2013-04-17 | Hanmi Science Co., Ltd. | A pharmaceutical composition comprising an immunoglobulin fc region as a carrier |
CN101883784B (zh) * | 2007-10-01 | 2014-10-15 | 药华医药股份有限公司 | N-端修饰的干扰素-α |
FR2964434B1 (fr) | 2010-09-07 | 2012-08-24 | Walden Associates Ltd S A | Amortisseur a haut pouvoir dissipatif et pratiquement sans huile |
JP6284478B2 (ja) | 2011-09-05 | 2018-02-28 | ハンミ サイエンス カンパニー リミテッド | インターフェロンアルファ結合体を含む癌治療用医薬組成物 |
US11471497B1 (en) | 2019-03-13 | 2022-10-18 | David Gordon Bermudes | Copper chelation therapeutics |
US10973908B1 (en) | 2020-05-14 | 2021-04-13 | David Gordon Bermudes | Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE78515T1 (de) | 1984-10-05 | 1992-08-15 | Genentech Inc | Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. |
JPS63230089A (ja) | 1987-03-18 | 1988-09-26 | Takeda Chem Ind Ltd | 分泌発現による蛋白質の製造法 |
EP0626448A3 (de) * | 1993-05-26 | 1998-01-14 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verfahren zur Herstellung und Reinigung von alpha-Interferon |
US5470719A (en) * | 1994-03-18 | 1995-11-28 | Meng; Shi-Yuan | Modified OmpA signal sequence for enhanced secretion of polypeptides |
KR100316347B1 (ko) * | 1998-09-15 | 2002-08-27 | 한미약품(주) | 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 |
-
2000
- 2000-01-19 KR KR1020000002434A patent/KR100360594B1/ko active IP Right Grant
-
2001
- 2001-01-19 DE DE60130100T patent/DE60130100T2/de not_active Expired - Lifetime
- 2001-01-19 WO PCT/KR2001/000097 patent/WO2001057217A1/en active IP Right Grant
- 2001-01-19 NZ NZ520076A patent/NZ520076A/en not_active IP Right Cessation
- 2001-01-19 BR BRPI0107708A patent/BRPI0107708A8/pt not_active Application Discontinuation
- 2001-01-19 CN CNB018038603A patent/CN100436581C/zh not_active Expired - Fee Related
- 2001-01-19 US US10/182,040 patent/US7052867B2/en not_active Expired - Lifetime
- 2001-01-19 RU RU2002122088/13A patent/RU2230116C2/ru not_active IP Right Cessation
- 2001-01-19 AT AT01949005T patent/ATE371027T1/de not_active IP Right Cessation
- 2001-01-19 ES ES01949005T patent/ES2290156T3/es not_active Expired - Lifetime
- 2001-01-19 JP JP2001558031A patent/JP3751883B2/ja not_active Expired - Fee Related
- 2001-01-19 AU AU28909/01A patent/AU762053B2/en not_active Ceased
- 2001-01-19 EP EP01949005A patent/EP1250439B1/en not_active Expired - Lifetime
- 2001-01-19 CA CA2397527A patent/CA2397527C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
ES2290156T3 (es) | 2008-02-16 |
AU762053B2 (en) | 2003-06-19 |
JP2003521925A (ja) | 2003-07-22 |
BRPI0107708A8 (pt) | 2016-06-07 |
BR0107708A (pt) | 2002-11-19 |
US20040151695A1 (en) | 2004-08-05 |
KR100360594B1 (ko) | 2002-11-13 |
JP3751883B2 (ja) | 2006-03-01 |
WO2001057217A9 (en) | 2002-12-27 |
WO2001057217A1 (en) | 2001-08-09 |
KR20010073658A (ko) | 2001-08-01 |
CA2397527C (en) | 2010-10-19 |
EP1250439A1 (en) | 2002-10-23 |
EP1250439B1 (en) | 2007-08-22 |
DE60130100D1 (de) | 2007-10-04 |
ATE371027T1 (de) | 2007-09-15 |
NZ520076A (en) | 2003-07-25 |
AU2890901A (en) | 2001-08-14 |
EP1250439A4 (en) | 2004-04-07 |
US7052867B2 (en) | 2006-05-30 |
CA2397527A1 (en) | 2001-08-09 |
DE60130100T2 (de) | 2008-05-15 |
CN100436581C (zh) | 2008-11-26 |
RU2230116C2 (ru) | 2004-06-10 |
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Owner name: KOREA-US HOLDINGS CO., LTD. Free format text: FORMER OWNER: HANMI PHARM. IND. CO., LTD. Effective date: 20110328 |
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Effective date of registration: 20110328 Address after: Seoul, South Kerean Patentee after: Hanmi Pharm Ind Co.,Ltd. Address before: Gyeonggi Do, South Korea Patentee before: Hanmi Pharm. Ind. Co., Ltd. |
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Owner name: HANMI SCIENCE CO., LTD. Free format text: FORMER NAME: HANMI HOLDINGS CO., LTD. |
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Address after: Gyeonggi Do, South Korea Patentee after: Hanmi Holdings Co., Ltd. Address before: Seoul, South Kerean Patentee before: Hanmi Pharm Ind Co.,Ltd. |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081126 Termination date: 20200119 |
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CF01 | Termination of patent right due to non-payment of annual fee |