CN1415189A - Method for high performance collective breeding non-nuclear grape with disease resistant capability - Google Patents
Method for high performance collective breeding non-nuclear grape with disease resistant capability Download PDFInfo
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- CN1415189A CN1415189A CN 02139331 CN02139331A CN1415189A CN 1415189 A CN1415189 A CN 1415189A CN 02139331 CN02139331 CN 02139331 CN 02139331 A CN02139331 A CN 02139331A CN 1415189 A CN1415189 A CN 1415189A
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Abstract
An efficient aggregate method for breeding seedless grap is disclosed, which features that the conventional hybridizing technique, the embryo retriever technique and molecular marker breeding technique are integrated. Its advantages are short breeding period shortened from 7-9 years to 3-4 months, high resistance to disease, high seedless percentage, and high quality of grape.
Description
Technical field
The present invention relates to the plant breeding technology, particularly relate to a kind of disease-resistant currant high-efficiency polymerization breeding method.
Background technology
Grape is as worldwide fruit, and its cultivated area and output all occupy the second in the world.And seedless proterties is a kind of special proterties sign in the grape commodity production, and in developed country and area, currant output has accounted for more than 60% of grape production.In China, along with the increase of market demand, the area under cultivation of currant is also in rising trend, but because of the hysteresis of raisin grape breeding technology and the shortage of new varieties, has seriously hindered the raising of currant output and quality.At present, worldwide traditional conventional hybridization breeding technique is generally adopted in the breeding of currant, promptly maternal there to be the nuclear kind to do, after making paternal hybrid with seedless variety, sowing separates, and by the offspring is selected with seed selection currant kind, this method efficient is extremely low, and the seedless proterties of identifying hybrid needs can carry out after 3～5 annual bearings at least, and the breeding method of this poor efficiency consuming time can not meet the needs of production far away.
The stripped embryo rescue techniques of U.S.'s " horticultural science " magazine nineteen ninety 25 volume the 4th phase 393～398 pages of reports, improved the ratio that the seedless hybrid of currant intervarietal cross offspring occurs, more conventional conventional hybridization technology is compared simultaneously, shortened for 4～5 years, but the observation to filial generation purpose proterties is by just carrying out behind the embryo redemption seedling field planting land for growing field crops result, do not carry out molecular marker assisted selection in the seedling phase, thereby efficient is lower, and the currant kind of seed selection lacks disease resistance.Nineteen ninety reported first the DNArandom amplified polymorphic DNA dna technology as the molecular marking technique of plant breeding, obtain fast development in the molecular biology research field, and significant results have been obtained in practice, utilize this technology to seek the molecular labeling of heredity, can reach quick, easy, cost is low, rich polymorphism, do not need tagging and safe and reliable effect again, thereby be subjected to the extensive attention of world molecular biosciences educational circles, but this technology only belongs to a kind of assistant breeding technology at present, also is not widely used in the breeding technical field of disease-resistant currant.
Summary of the invention
Purpose of the present invention will overcome exactly utilizes conventional conventional hybridization breeding technique to cultivate long, inefficient shortcoming of disease-resistant currant cycle, invented a kind of conventional hybridization technology, embryo rescue techniques, molecular mark technology of melting and be used to cultivate the high-efficiency polymerization breeding method system of disease-resistant currant new varieties, fundamentally improved the breeding efficiency of disease-resistant currant in one.
It is maternal that the present invention adopts the good currant kind of economic characters to do, with the strong kind of disease resistance in the Chinese wild grape, strain system or hybrid carry out field artificial hybridization as male parent, after 40～53 days, adopt the immature hybridization ovule embryo that exsomatizes in the ovule to save, save seedling with the embryo that obtains a large amount of filial generations, the a large amount of disease-resistant currant embryo that obtains is saved the seedling offspring, with the DNArandom amplified polymorphic DNA DNA (deoxyribonucleic acid) mark that detects grape kernal eliminating gene and disease-resistant gene, carry out disease-resistant proterties of seedling phase molecular marker assisted selection and seedless proterties, to obtain disease-resistant currant new varieties.
Utilize the present invention to cultivate disease-resistant currant new varieties, only needed 3～4 months can obtain embryo redemption seedling, at least shorten 7～9 years time altogether than identifying behind conventional hybridization breeding and the conventional wait hybrid seedling result, improved breeding efficiency greatly, reach rank first of the same trade, for making full use of the peculiar abundant amur grape disease-resistant gene of China, fast cultivate the disease-resistant currant new varieties of high-quality and started bright prospects.
Specific implementation method
A. make male parent with the strong kind of Chinese wild grape disease resistance, strain system or hybrid, gathered its pollen in preceding 1～2 day blooming.
B. the pollen oven dry grinding of gathering is loaded in the vial, it is standby bottle to be placed in the drier put into-20 ℃ of refrigerators to preserve.
C. be female parent with high-quality Europe currant kind, blooming preceding 2～3 days to the flower castration, water spray back bagging.
D. rise next day with standby pollen castrating the flower pollination, pollinated continuously three days, after the pollination immediately to its bagging, to form berry.
E. pollination back is 40～53 days, earlier berry is washed repeatedly with clear water, and the back with 20～30 seconds of 70% alcohol rinsing, is put into 0.1% mercuric chloride thimerosal again and soaked 6～8 minutes on superclean bench, washes repeatedly with sterile water then.
F. the berry after will sterilizing strips ovule under aseptic condition, be inoculated in culturing room on the medium of being made by the embryonic development culture fluid and impel embryo to reach full growth.
F.1 culturing room's temperature is 25～28 ℃.
F.2 illumination is 16 hours.
F.3 intensity of illumination 2000～3000 luxs.
G. cultivate after 55～60 days the aseptic rataria that strips in culturing room, be inoculated in and impel its sprouting on the medium of making by the embryo germination culture fluid.
G.1 culturing room's temperature is 25～28 ℃.
G.2 illumination is 12 hours.
G.3 intensity of illumination 1000～1500 luxs.
H. the rataria of Meng Faing is cultivated into embryo and is saved seedling on being inoculated in by the medium that becomes the seedling culture fluid to make after 10～15 days in culturing room.
H.1 culturing room's temperature is 25～28 ℃.
H.2 illumination is 12 hours.
H.3 intensity of illumination 2000～3000 luxs.
The one-tenth seedling light hardening 6～7 days that j. will have 4～5 leaves.
K. under the aseptic condition seedling moved on to hardening in the perlite.
K.1 humidity 80%～95%.
K.2 800～1000 times 25% carbendazim is diluted in pouring.
L.15 day after seedling moved on in the nutrition soil in the greenhouse hardening 3～4 months.
M. be colonizated in the land for growing field crops spring next year.
N. embryo is saved seedling and carry out the extraction of genomic deoxyribonucleic acid.
O. utilize the DNArandom amplified polymorphic DNA DNA (deoxyribonucleic acid) mark that detects grape kernal eliminating gene and disease-resistant gene, in the seedling phase embryo is saved seedling and carry out disease-resistant proterties of molecular marker assisted selection and seedless proterties, to eliminate the filial generation of non-objective trait.
Claims (2)
1, a kind of disease-resistant currant high-efficiency polymerization breeding method, it is maternal to it is characterized in that adopting the good currant kind of economic characters to do, with the strong kind of disease resistance in the Chinese wild grape, strain or hybrid carry out field artificial hybridization as male parent, after 40～53 days, adopt the immature hybridization ovule embryo that exsomatizes in the ovule to save, save seedling to obtain a large amount of filial generation embryos, the a large amount of disease-resistant currant embryo that obtains is saved the seedling offspring, with the DNArandom amplified polymorphic DNA DNA (deoxyribonucleic acid) mark that detects grape kernal eliminating gene and disease-resistant gene, carry out disease-resistant proterties of molecular marker assisted selection and seedless proterties, to obtain disease-resistant currant new varieties.
2, a kind of disease-resistant currant high-efficiency polymerization breeding method according to claim 1 is characterized in that taking following specific implementation method:
2.a make male parent with the strong kind of Chinese wild grape disease resistance, strain system or hybrid, gathered its pollen in preceding 1～2 day blooming,
2.b the pollen oven dry grinding of gathering is loaded in the vial, and it is standby bottle to be placed in the drier put into-20 ℃ of refrigerators to preserve,
2.c with European high-quality currant kind is female parent, is blooming preceding 2～3 days to the flower castration, water spray back bagging,
2.d rise next day with standby pollen to castrating the flower pollination, pollinated continuously three days, after the pollination immediately to its bagging, forming berry,
2.e pollination back 40～53 days is washed berry with clear water earlier repeatedly, the back with 20～30 seconds of 70% alcohol rinsing, is put into 0.1% mercuric chloride thimerosal again and was soaked 6～8 minutes on superclean bench, wash repeatedly with sterile water then,
2.f the berry after will sterilizing strips ovule under aseptic condition, in culturing room, is inoculated on the medium of making by the embryonic development culture fluid to impel embryo to reach full growth,
2.f.1 25～28 ℃ of culturing room's temperature,
2.f.2 illumination 16 hours,
2.f.3 intensity of illumination 2000～3000 luxs,
2.g cultivate after 55～60 days the aseptic rataria that strips in culturing room, be inoculated in and impel its sprouting on the medium of making by the embryo germination culture fluid,
2.g.1 25～28 ℃ of culturing room's temperature,
2.g.2 illumination 12 hours,
2.g.3 intensity of illumination 1000～1500 luxs,
2.h the rataria of sprouting on being inoculated in by the medium that becomes the seedling culture fluid to make after 10～15 days in culturing room, being cultivated into embryo and is saved seedling,
2.h.1 25～28 ℃ of culturing room's temperature,
2.h.2 illumination 12 hours,
2.h.3 intensity of illumination 2000～3000 luxs,
2.j will have the one-tenth seedling light hardening 6～7 days of 4～5 leaves,
2.k under the aseptic condition seedling moved on to hardening in the perlite,
2.k.1 humidity 80%～95%,
2.k.2 25% carbendazim that the pouring dilution is 800～1000 times,
2.l15 after it seedling moved on in the nutrition soil in the greenhouse hardening 3～4 months,
2.m be colonizated in the land for growing field crops spring next year,
2.n being saved seedling, embryo carries out the extraction of genomic deoxyribonucleic acid,
2.o utilize the DNArandom amplified polymorphic DNA DNA (deoxyribonucleic acid) mark that detects grape kernal eliminating gene and disease-resistant gene, in the seedling phase embryo saved seedling and carry out disease-resistant proterties of molecular marker assisted selection and seedless proterties, to eliminate the filial generation of non-objective trait.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNB021393311A CN1176575C (en) | 2002-08-05 | 2002-08-05 | Method for high performance collective breeding non-nuclear grape with disease resistant capability |
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| CNB021393311A CN1176575C (en) | 2002-08-05 | 2002-08-05 | Method for high performance collective breeding non-nuclear grape with disease resistant capability |
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| CN1415189A true CN1415189A (en) | 2003-05-07 |
| CN1176575C CN1176575C (en) | 2004-11-24 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536669B (en) * | 2009-04-15 | 2011-07-20 | 西北农林科技大学 | High-efficiency polymerization method for apple cross breeding |
| CN102370127A (en) * | 2010-08-25 | 2012-03-14 | 杨声谋 | Method for industrial production of fresh and alive pollen by centralized growing of male flowers of kiwi fruit |
| CN104988179A (en) * | 2015-06-30 | 2015-10-21 | 中国农业科学院郑州果树研究所 | Biotechnological breeding method for obtaining antiviral seedless grapes |
| CN107723378A (en) * | 2017-11-13 | 2018-02-23 | 中国农业科学院郑州果树研究所 | The seedless main effect QTL site SDL of grape fruit SNP marker and application |
| CN107760798A (en) * | 2017-11-13 | 2018-03-06 | 中国农业科学院郑州果树研究所 | The SSR molecular marker in the seedless main effect QTL site of grape fruit |
| CN110402753A (en) * | 2019-04-03 | 2019-11-05 | 新疆农业科学院园艺作物研究所 | A kind of method of new grape variety quickly breeding |
| CN112167047A (en) * | 2020-08-20 | 2021-01-05 | 郑州启汇果品有限公司 | Method for cultivating new variety of grapes, namely Kaura rose grapes |
| CN112167046A (en) * | 2020-08-20 | 2021-01-05 | 郑州启汇果品有限公司 | Method for cultivating new variety of grapes, Eimei grapes |
| CN112167049A (en) * | 2020-08-20 | 2021-01-05 | 郑州启汇果品有限公司 | Method for cultivating novel variety of grapes, namely Xueshan rose grapes |
-
2002
- 2002-08-05 CN CNB021393311A patent/CN1176575C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536669B (en) * | 2009-04-15 | 2011-07-20 | 西北农林科技大学 | High-efficiency polymerization method for apple cross breeding |
| CN102370127A (en) * | 2010-08-25 | 2012-03-14 | 杨声谋 | Method for industrial production of fresh and alive pollen by centralized growing of male flowers of kiwi fruit |
| CN102370127B (en) * | 2010-08-25 | 2013-09-11 | 杨声谋 | Method for industrial production of fresh and alive pollen by centralized growing of male flowers of kiwi fruit |
| CN104988179A (en) * | 2015-06-30 | 2015-10-21 | 中国农业科学院郑州果树研究所 | Biotechnological breeding method for obtaining antiviral seedless grapes |
| WO2017000089A1 (en) * | 2015-06-30 | 2017-01-05 | 中国农业科学院郑州果树研究所 | Biotechnological breeding method for obtaining antiviral seedless grapes |
| CN107760798A (en) * | 2017-11-13 | 2018-03-06 | 中国农业科学院郑州果树研究所 | The SSR molecular marker in the seedless main effect QTL site of grape fruit |
| CN107723378A (en) * | 2017-11-13 | 2018-02-23 | 中国农业科学院郑州果树研究所 | The seedless main effect QTL site SDL of grape fruit SNP marker and application |
| CN107723378B (en) * | 2017-11-13 | 2019-11-05 | 中国农业科学院郑州果树研究所 | The SNP marker and application of the seedless main effect QTL site SDL of grape fruit |
| CN107760798B (en) * | 2017-11-13 | 2020-10-23 | 中国农业科学院郑州果树研究所 | SSR molecular marker of seedless major QTL (quantitative trait locus) of grape fruit |
| CN110402753A (en) * | 2019-04-03 | 2019-11-05 | 新疆农业科学院园艺作物研究所 | A kind of method of new grape variety quickly breeding |
| CN112167047A (en) * | 2020-08-20 | 2021-01-05 | 郑州启汇果品有限公司 | Method for cultivating new variety of grapes, namely Kaura rose grapes |
| CN112167046A (en) * | 2020-08-20 | 2021-01-05 | 郑州启汇果品有限公司 | Method for cultivating new variety of grapes, Eimei grapes |
| CN112167049A (en) * | 2020-08-20 | 2021-01-05 | 郑州启汇果品有限公司 | Method for cultivating novel variety of grapes, namely Xueshan rose grapes |
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| Publication number | Publication date |
|---|---|
| CN1176575C (en) | 2004-11-24 |
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