CN1397567A - Spiruline polyose, its extraction process and it medical application in increasing white cells and treating cancer - Google Patents
Spiruline polyose, its extraction process and it medical application in increasing white cells and treating cancer Download PDFInfo
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Abstract
A spirulina polyose is prepared through extracting polyose, extracting liquid protein, centrifugal separation, concentrating supernatant, depositing in alcohol, removing insoluble substances, deposition of supernatant in alcohol, and drying. Its advantages are high polyose content (60% or more), and high effect on inhibiting the damage to hematopoietic function of narrow during radiotheraphy and chmicotherapy and increasing white cells.
Description
Invention field
The present invention relates to spirulina polysaccharide, its extracting method and rise purposes in white and the cancer therapy drug in preparation.
Background technology
Spirulina (Spirulina platensis) has another name called blue-green algae, have nourishing, strong, replenishing qi and blood, reduce phlegm, the effect of soft heavily fortified point, it is rich in abundant VITAMIN, protein, unsaturated fatty acids, nucleic acid, trace element, especially its polysaccharide of containing, have multiple biological activity, have purposes widely in the medicines and health protection field.
In recent decades, the Chinese medicinal materials spirulina plalensis has been carried out a series of pharmacology and clinical study both at home and abroad, the result shows: multiple pharmacological effect such as spirulina plalensis has leukocyte increasing, enriches blood, enhance immunity function, regulating blood fat, anticancer, Antiradiation injury.
Leukopenia is clinical rather common, its pathogenic factor is a lot, particularly in the treatment of tumour patient, because of postoperative body empty and put, chemotherapy is more thorny to the toxicity of hemopoietic function of bone marrow, and the leukogenic effect of current Chinese medicine preparation remains further to be improved, and Western medicine has certain side reaction again.
Though bibliographical information the leukogenic effect of spirulina, separately spirulina polysaccharide is not carried out the research of this respect.The inventor finds that after deliberation spirulina polysaccharide has significant effect in increasing leukocyte, and its curative effect is better than Berbamine, especially can be used for the oligoleukocythemia behind the chemicotherapy.And, the effect that also has significant inhibition tumour.
Summary of the invention
The purpose of this invention is to provide the spirulina polysaccharide extract, and new preparation process and rise purposes in white and the antitumor drug in preparation.
Spirulina polysaccharide extract of the present invention mainly is on pharmacology that the known helical algae has and the effective basis of clinical effect, adopt the theory and the method for modern biotechnology, extract efficient part and the white and antitumor drug of the liter with higher level made from the single spirulina that proves to be really effective.
With the polyoses extract of the inventive method preparation, polysaccharide content is greater than 60%.Mass yield is between 4.2～4.6%.
Preparing polyoses extract of the present invention may further comprise the steps:
A, polysaccharide extract, and take that the lixiviate of diluted alkaline temperature is followed the example of, the lixiviate of aqueous solution temperature is followed the example of or the lixiviate of salts solution temperature is followed the example of, and the lixiviate of preferred aqueous solutions temperature is followed the example of;
B, extracting solution are transferred the albumen iso-electric point, and be centrifugal then;
C, centrifugate are got supernatant concentration to finite concentration, adopt ethanol sedimentation, and insolubles is removed in centrifugal or filtration;
D, supernatant liquor carry out the secondary ethanol sedimentation, and be dry again, gets the spirulina polysaccharide extract.
About extraction method of polysaccharides, can consult the Zhang Weijie chief editor: complex polysaccharide Biochemical Research technology, Shanghai science tech publishing house, 1987.Wu Wutong chief editor: bio-pharmaceuticals technology, Chinese Medicine science and technology press,, 385 pages in 1993.
Wherein extract generally doubly with 4-10, the extracting solution that preferably about 7-8 doubly measures, at 70-90 ℃, under preferably about 85 ℃ temperature about 7-10 hour.Can extract 1-3 time.
The albumen iso-electric point generally transfers to 4-about 6.5, preferred about 5.0.
Centrifugal speed usually between 3000-7500 rev/min, preferred about 3600 rev/mins.
The alcohol precipitation concentration of polysaccharide between about 50%～80%, preferred 75%.
Spissated multiple 2-6 doubly between, preferred 4 times.
Polyoses extract is preferable over 60-70 ℃, and preferred 60 ℃, (about 0.090Mpa) dry 5-9h under the vacuum, preferred 8h carries out drying, so can make the water content of polysaccharide be lower than 9%.But also can carry out drying, but temperature can not be too high by usual way.
The present invention utilizes the hot water lixiviate of the water-soluble characteristics of polysaccharide, not only can make polysaccharide discharge, dissolve, and can shorten extraction time, prevents corruption and makes protein denaturation.
According to the principle of albumen solubleness minimum when its iso-electric point, extracting solution is transferred pH5.0, and a large amount of foreign proteins are removed albumen precipitation with centrifugation method then in the precipitation extracting solution.
Utilize polysaccharide to be insoluble in the characteristics of high concentration ethanol, use the ethanol sedimentation polysaccharide; Adopt 75% ethanol sedimentation polysaccharide can also remove the polypeptide of a large amount of inorganic salt, monose, amino acid and small molecular weight simultaneously.
TLC and GC analysis revealed: polysaccharide mainly is made up of rhamnosyl, Fucose, three kinds of monose of glucose in this polyoses extract.Its mol ratio is (3.9: 1.4: 11.3).Molecular weight is 3,390～2,907,623, and this polysaccharide main chain is based on glucose, and there is multiple bind mode in each monose.Prove that by color reaction and Infrared spectroscopy uronic acid group and sulfate group are arranged on this polysaccharide chain.Assay: with reference to colorimetric method for determining in 95 editions appendix IV of Chinese Pharmacopoeia spectrophotometry.
Require to have studied the preliminarily stabilised of spirulina polysaccharide extract according to Bureau of Drugs Supervision's provisions for new drugs approval.At room temperature, three lot numbers 970710,970815,970912 have been carried out 0 month, January, February, March investigated, the investigation project is proterties, discriminating, moisture, ash content, pH value, mould total plate count and assay.Every index all meets the requirements.Conclusion: the spirulina polysaccharide extract is stable at normal temperatures.
Spirulina polysaccharide extract Pharmacodynamic test of active extract is carried out in requirement by " herbal medicine efficacy is learned the research guide ".Test-results shows: (1) spirulina polysaccharide extract 15～60mg/kg/d, and administration was dose-dependently in 12 days, made CTX induced mice marrow dna content, and karyocyte number and peripheral white blood cell reduce.Effect is better than the effect of positive drug SHENGBAIAN PIAN.
(2) spirulina polysaccharide extract 12mg/kg/d administration in advance is 5 days, and in the 7th day (the most obvious phase of bone marrow depression) of irradiation, total administration 26 days, right
60Dog marrow granulocyte system's karyocyte ratio that the Co-gamma-rays causes and peripheral blood cells number reduce obvious preventive and therapeutic effect.
(3) the spirulina polysaccharide extract is to medicine (CTX) and radioactivity
60Damage of Co-gamma-rays hemopoietic function of bone marrow and inhibition have tangible preventive and therapeutic effect.
And carry out toxicity test, the result is as follows:
(1) acute toxicity test in mice
The spirulina polysaccharide solution of extract with maximum concentration, maximum volume (greater than 5000mg/kg) give mouse once gavage with abdominal injection after, observed continuously 7 days, do not see that unusual performance and dead takes place animal, its maximum tolerated dose is decided to be 5000mg/kg.
(2) rat long term toxicity test
The spirulina polysaccharide extract gavages by 240mg/kg, 40mg/kg respectively, successive administration 90 days and 180 days, and after the administration, the general situation of all animals, blood routine, biochemical indicator, obvious ANOMALOUS VARIATIONS does not all appear in main organs coefficient and techtology.
(3) long term toxicity test of dog
The spirulina polysaccharide extract is fed by 20mg/kg and 200mg/kg respectively and is raised dog, and successive administration is after 6 months, and obvious ANOMALOUS VARIATIONS does not all appear in general situation, electrocardiogram(ECG, hematology and blood parameters, the histopathologic examination of all animals.
Specific embodiment
Add dry algae powder of 40kg 100 orders and 320L water in 500L lass lining jar, stir evenly, temperature was 85 ℃ in chuck was heated to, and insulation was extracted 8 hours, stirred 10 minutes to keep all even homo(io)thermism everywhere of extracting solution in per 0.5 hour in the leaching process.Temperature is soaked and is finished postcooling to room temperature, regulates pH5.0 with 4mol/L HCl and 4mol/L NaOH, and leave standstill 1h and treat a large amount of albumen precipitations, the centrifugation of SS-600 whizzer, centrifugal residue employing is just put forward condition and is extracted the centrifugal secondary residue that discards again 1 time.Clear liquid merges the back and be evaporated to 1/4th of original volume under 70 ℃, 0.085MPa vacuum tightness in 500L thin film concentration equipment, altogether the 140L concentrated solution (conversion is d
20Rise to 1.28g/ml from 1.12g/ml).After treating that solution is cooled to room temperature, adding 392L 95% medicinal alcohol, to make determining alcohol be 75%, leave standstill more than 8 hours, centrifuging, and with 40L 95% medicinal alcohol at twice behind the drip washing filter cake filter do.Precipitation (3.5kg) is also filtered with 15 times of (52.5L) water dissolution, and it is 75% precipitation polysaccharide that filtrate adds 95% ethanol to alcohol concn, leaves standstill, filters, with a small amount of 95% medicinal alcohol washing precipitation.The wet solid of gained is dry 8h under 60 ℃, 0.090MPa vacuum tightness.Mass yield is between 4.2～4.6%, and promptly every 1000g algae powder raw material can get 42～46g spirulina polysaccharide extract.Measure sugared content greater than 60% with sulfuric acid-phynol method.
The quality investigation of test one, polysaccharide
1, the qualitative analysis of polysaccharide
(1) takes by weighing spirulina polysaccharide extract 1g and add 50ml water, make the aqueous solution.Get 5ml and in test tube, add 10% naphthyl alcohol ethanol test solution 2-3 and drip, shake up the back and add vitriol oil 0.5ml, between two liquid interfaces, be brown ring along tube wall.
(2) get above-mentioned sugar soln 1ml in addition and add the 1ml 6% phenol test solution and the 5ml vitriol oil in test tube, shake up, solution is pale brown look.
2, determination of polysaccharide
Get 6 lot numbers of pilot product, 100mg decided in accurate respectively title, put in the little funnel that oneself is placed with filter paper, wash with 95% hot ethanol (70 ℃) 20ml, put into the 100ml Erlenmeyer flask in the lump after residue and filter paper flung to ethanol, add water 40ml and on electric furnace, be heated to little boiling, stirring and dissolving adds water 30ml along the bottle wall again, sweeps away the polysaccharide that adheres on it, continuing to be heated to little boiling kept several minutes, filtered while hot is divided washing Erlenmeyer flask and filter paper three times with 70 ℃ of water 20ml to the 100ml measuring bottle, put cold back water and be settled to scale, therefrom draw the 2.0ml thin up to 50ml, as need testing solution.Accurate in addition title is decided 101.2mg dextrose anhydrous reference substance and is dissolved in the 100ml measuring bottle, adds water to scale, and suction 5.0ml to the 100ml volumetric flask, is settled to scale again.Be prepared into solution that every 100ml contains 5.06mg glucose product solution in contrast.
The accurate need testing solution 2.0ml that draws; Reference substance solution 0.4,0.8,1.2,1.6,2.0ml, the not enough 2.0ml of reference substance solution adds water to 2.0ml; Blank water intaking 2.0ml replaces glucose solution.Add phenol test solution 1.0ml respectively, shake up, add vitriol oil 5.0ml again, shake up, put in the cold water and cooled off 5 minutes, put again in the boiling water and heated 20 minutes, take out flowing water and be cooled to room temperature, press spectrophotometry (Pharmacopoeia of People's Republic of China, 51 pages of appendix of nineteen ninety-five version) operation, measure the optical density of trial-product and reference substance at the 490nm place, calculate content with accompanying typical curve, calculation formula is as follows:
Polysaccharide content %=C * D * 1/W * K * 100
Wherein: the sample that C-is calculated by typical curve is equivalent to the micrograms of glucose, μ g
The D-extension rate
The W-example weight, μ g
K=0.9, n-1 H sloughs by n monose in polysaccharide system
2O is polymerized, its mass ratio
Be about 1: 0.9.
The glucose typical curve sees Table 1.
Table 1 glucose typical curve
Sequence number 1234 5C
Gluc, μ g/ml 20.24 40.48 60.72 80.96 101.2 A
4900.117 0.230 0.352 0.470 0.598 is had do linear regression by data in the table 1:
C (μ g/2ml)=1.24+168A
490, the polysaccharide content in six batches of pilot products of r=0.9997 sees Table 2.
Polysaccharide content sample number into spectrum 970,710 970,815 970,912 991,008 991,020 991102 (mg) 101.7 98.7 100.2 98.9 96.5 102.4 A that weigh in table 2 sample
4900.307 0.323 0.291 0.302 0.283 0.307 content (%) 64.9 70.3 62.5 65.7 63.2 64.4 as known from Table 2: the polysaccharide content of 6 batches of products is all greater than 60%.The influence that test two, spirulina polysaccharide extract suppress the caused by cyclophosphamide mouse hemopoietic system
1, endoxan (CTX) is caused the influence that peripheral blood cells reduces
Get 60 of mouse, be divided into 6 groups at random.Control group is irritated stomach SCMC (15ml/kg) every day, and model group is irritated stomach SCMC (15ml/kg) same every day.Irritate stomach spirulina polysaccharide extract 15mg, 30mg or 60mg/kg/d every day in being subjected to the reagent group.The positive drug group gavages with SHENGBAIAN PIAN (Wuxi City the 6th pharmaceutical factory's product, lot number 960112) 60mg/kg (SCMC suspension).Successive administration 12 days.Administration is in the time of the 5th day, except that control group, all the other are respectively organized mouse and all give intraperitoneal injection of cyclophosphamide (CTX) (Shanghai No.12 Pharmaceutical Factory, lot number 960501) 100mg/kg, once a day, for three days on end, continue administration simultaneously every day, in medication the 12nd day (total fate), get blood system from mouse orbit and do not count WBC, RBC and measure HB (table 1).The result shows that endoxan can make mouse peripheral blood leucocyte (WBC), and red corpuscle (RBC) and oxyphorase (HB) obviously reduce.The WBC that spirulina polysaccharide extract 15mg～60mg/kg/d administration 12 angels reduce raises 15～60%, and effect is better than the positive control drug SHENGBAIAN PIAN, and RBC and the HB that reduces do not had obvious rising effect.
2, CTX is caused the influence of mouse bone marrow cells dna content and the minimizing of karyocyte number
Get 60 of mouse, grouping administration and model method are the same.Administration the 12nd day is got and is respectively organized mouse both sides femur, carries out marrow dna content mensuration and karyocyte counting respectively.
The marrow dna content is measured: get the right side femur, the Ex-all soft tissue.Use 0.005mol/LCaCl
210ml pours whole marrow in the centrifuge tube.Put 4C refrigerator 30min, the centrifugal 15min of 2500rpm.Abandon supernatant, throw out is added 0.2mol/L HClO
4The abundant mixing of 5ml, 90 ℃ of heating 15min, cold filtration, filtrate is measured ultraviolet absorption value (1OD with ultraviolet spectrophotometer in 260nm
260=50 μ g/ml double-stranded DNAs), dna content in the final filtrate of 5ml (dna content in the every femur)=OD value * 50 * 5, the μ g/ of unit femur.
The bone marrow nucleated cell counting: get the left side femur, go out medullary cell with 10ml 3% acetic acid, the cell count of four big grids of counting on the cytometry dish, the number of gained multiply by 2.5 * 10
4, be a bone marrow nucleated cell number in the femur and (annotate: 2.5 * 10
4The expression extension rate.The volume of four big grids is 0.4mm
3, 10ml=10000mm
3, 10000/0.4=2.5 * 10
4).
The result shows that CTX can make mouse bone marrow cells dna content and karyocyte number obviously reduce, and presents the apparent myelosuppression effect.The spirulina polysaccharide extract can make the marrow dna content of minimizing and karyocyte number be gone up, and its curative effect is better than SHENGBAIAN PIAN.
Test three, the spirulina polysaccharide extract is right
60The influence that the dog hemopoietic system suppresses due to the Co-gamma-rays
1, right
60The influence that the dog peripheral blood cells reduces due to the Co-gamma-rays
Get 20 of hybrid dogs, be divided into 5 groups at random.Experimentize after conventional one week of raising.Administering mode is raised for the pharmaceutical drying powder being clipped in boil to feed in the Radix Polygalae Crotalarioidis section.The normal control group and
60Co-gamma-radiation group (model group) is only fed and is given the chitling that does not press from both sides medicine.Be subjected to the reagent group to sandwich food by 3mg and 12mg/kg respectively with spirulina polysaccharide extract dried powder and feed and to raise, the positive drug group is fed and is raised SHENGBAIAN PIAN (12mg/kg).Above-mentioned administration once a day, continuous 26 days.The 5th day usefulness of administration
60The Co-gamma-rays is to shielding pelvis dog irradiation 6.5Gy (dose rate be 100 human relations/minute).Concrete grammar is a pre-irradiation with 7 of 20 * 10 * 5 (cm, length * wide * height) lead bricks, lies against 2cm place before the pelvis,
60Co-gamma-rays bilateral irradiation (also shielding pelvis during the irradiation opposite side) with method.It is 6.5Gy that the unshielded place of animal organizes absorption dose.Administration is continued in the irradiation back.And get blood with syringe at the subcutaneous cephalic vein of forelimb place respectively at irradiation back the 7th, 14 and 21 day, and count WBC respectively, RBC and measure HB, the result shows
60The Co-gamma-rays can make dog peripheral blood WBC, RBC and HB reduce, and is the most obvious to shine back the 7th day.
60Co-gamma-radiation group (model group) WBC descends 62.5%, and RBC descends 26.4%, and HB descends 18.9%.Spirulina polysaccharide extract 12mg/kg makes WBC, the RBC of reduction and HB raise 39.5%, 24% and 20% respectively.
2, right
60The influence that the dog bone marrow nucleated cell reduces due to the Co-gamma-rays
Get 20 of dogs, grouping, administration and model method are the same.In irradiation back the 7th, 14 and 21 day respectively at distal part of femur after popliteal flesh face, penetrate medullary space and draw bone marrow fluid with No. 16 marrow puncture needles, push into several marrow sheets, Wright's staining is checked bone marrow smear under oily mirror.The result shows
607 days bone marrow smear grains system and red system are obvious holddown behind the Co-gammairradiation.The granulocyte system karyocyte ratio (raising 250%, P＜0.01) that spirulina polysaccharide extract 12mg/kg can obviously raise and reduce, grain is 31% though 3mg/kg dosage group raises, difference is not remarkable.Spirulina polysaccharide extract 12mg/kg group also can make the red corpuscle system karyocyte ratio of reduction raise 50%, but difference is not remarkable, the results are shown in Table 3.
Table 3 spirulina polysaccharide extract is right
60The influence that the dog myeloid element reduces due to the Co-gamma-rays (x ± s)
Annotate: 1. karyocyte ratio %=karyocyte (individual)/mature erythrocyte (individual) * 100% (200 karyocytes of every marrow sheet counting).
| Group | The dog number | The karyocyte ratio % of granulocyte system | ||
| Shone back 7 days | 14 days | 21 days | ||
| The normal control group 60Co irradiation group 60Co+ Berbamine (12mg/kg) 60Co+ spirulina polysaccharide extract (3mg/kg) 60Co+ spirulina polysaccharide extract (12mg/kg) | ????4 ????4 ????4 ????4 ????4 | ??48.9±5.2 ??2.2±1.7 **??5.5±3.0 *??2.9±1.1 **??7.7±1.1 * Δ | ??46.3±6.1 ??10.1±3.6 **??20.4±2.9 Δ??16.3±1.7 *??22.3±2.9 * Δ | ??47.5±3.8 ??46.3±4.8 ??47.0±7.6 ??46.4±2.7 ??46.2±6.3 |
| The karyocyte ratio % of red corpuscle system | ||||
| Shone back 7 days | 14 days | 21 days | ||
| The normal control group 60Co irradiation group 60Co+ Berbamine (12mg/kg) 60Co+ spirulina polysaccharide extract (3mg/kg) 60Co+ spirulina polysaccharide extract (12mg/kg) | ????4 ????4 ????4 ????4 ????4 | ??32.9±3.8 ??14.3±5.8 *??18.0±2.9 **??14.0±3.7 **??21.5±4.2 ** | ??29.3±2.4 ??18.5±6.3 ??21.0±2.1 ??18.8±2.9 **??24.3±3.7 | ??30.8±3.0 ??27.0±5.5 ??28.8±4.9 ??29.0±6.2 ??31.0±3.5 |
2. compare * P＜0.05 with the normal control group,
*P＜0.01
3. with
60Co irradiation group relatively
ΔP＜0.05,
The Δ ΔP＜0.01
4. karyocyte ratio %＞10% is normal bone marrow and resembles (or increase year enliven bone marrow smear) conclusion
Spirulina polysaccharide extract (PSP) 15～60mg/kg/d, administration was dose-dependently in 12 days makes CTX induced mice marrow dna content, and karyocyte number and peripheral white blood cell reduce.Effect is better than the effect of positive drug SHENGBAIAN PIAN.
Spirulina polysaccharide extract (PSP) 12mg/kg/d administration in advance 5 days, and what shines the 7th day (the most obvious phase of bone marrow depression), total administration 26 days, right
60Dog medullary cell system karyocyte ratio that the Co-gamma-rays causes and peripheral blood cells number reduce obvious preventive and therapeutic effect.
Spirulina polysaccharide extract (PSP) to medicine (CTX) and radioactivity (
60The Co-gamma-rays) hemopoietic function of bone marrow damage and inhibition have tangible preventive and therapeutic effect.
Reference
1. Liu's Lik-Sang, etc.Spirulina polysaccharide is to the raising effect and the mechanism research thereof of body's immunity.Ocean science, 1991, (6): 44
2. Xu Hui, etc.The separation of spirulina polysaccharide and immunological role thereof.China's biochemical drug magazine, 1997, (2): 72-74
3. the left side fortune that continues, etc.Spirulina polysaccharide (PSP) is to Immune Effects.The medicine biotechnology, 1996, (3): 158-162
4. opening has the people, etc.Microcalorimetry research spirulina-active material is to cancer cells and Normocellular effect.Acta PhySico-Chimica Sinica, 1990; (2): 43
5. Liu's Lik-Sang, etc.Spirulina polysaccharide is to the restraining effect and the mechanism research of transplantability cancer cells.Ocean science, 1991; (5): 33-36
6. Chen Feng, etc.Spirulina is induced the restraining effect of large intestine sex change crypts to dimethylhydrazine.Chinese Journal of Preventive Medicine, 1995; (1): 13
7. open the Chengwu, etc.Spirulina plalensis polysaccharide and Phycocyanins, C-are to the protective effect of chmice acute radiation syndrome.Journal of Nutrition, 1996; (3): 327-331
8. Ruan Ji is red, etc.The radiation-resistant research of spirulina polysaccharide.Heredity, 1988; (1) 85-89
9. Pang Qi is dark, etc.Spirulina polysaccharide is repaired the synthetic enhancement to endonuclease activity and DNA.Acta Genetica Sinica, 1988; (5): 374-381
10. Liu Wei person of outstanding talent, etc.The strong blood sheet of the civilian spirulina in sea is to the influence of animal hemopoietic function.China's marine drug, 1996, (1): 19
11. the beam river is bright, etc.Spirulina is to the recovery experiment of hypoferric anemia.Journal of Nutrition, 1994; (4): 386
A fortune 12. left side continues, the experimental study of the anti-oxidant antifatigue effect of Yunnan spirulina polysaccharide.China's marine drug, 1995; (4): 18
13. Jiang Chun. the feature and the application thereof of heath food material one spiral shell polysaccharides.Sichuan foodstuffs industry science and technology, 1990, (2): 43
14. open Song Ren, etc., the preliminary study of spirulina prevention experimental rat stomach ulcer effect, Chinese marine drug, 1991; (4): 18
15. Xuchang is splendid, etc.Spirulina is to the influence of tumor radiotherapy patient blood plasma superoxide-dismutase.China's radiological medicine and protection magazine, 1996, (2): 136
16. Zhou Jianying, etc.Spiral algae sheet is to the research of chemotherapy malignant tumor patient provide protection.The Suzhou Medical College journal, 1995; (2): 223
17. it is splendid to be permitted to continue, etc.Spirulina is to the provide protection of tumor radiotherapy patient marrow and immunologic function.The Suzhou Medical College journal, 1995, (5): 814
18. Wang Hong is firm, etc., spirulina plalensis dialogue cytosis in the chemotherapy of acute leukemia.China's marine drug, 1996; (60): 42-44
19. bureau of drug policy ﹠ administration of Ministry of Health of the People's Republic of China.Traditional Chinese medicine research guide (pharmacy, pharmacology, toxicology)-preparation technology and research data thereof.1994,25 pages.
20. Zhang Weijie chief editor.Saccharide complex Biochemical Research technology.Press of Zhejiang University.1994.
21. Zhang Weijie chief editor.Complex polysaccharide Biochemical Research technology.Shanghai science tech publishing house.1987.
22. Wu Wutong chief editor.Bio-pharmaceuticals technology.Chinese Medicine science and technology press.1993,385 pages.
23. Xu Hui, Yu Zhijie, Su Fuqiang, etc.The separation of spirulina polysaccharide and immunological role thereof.China's biochemical drug magazine, 1997,18 (2): 72～80
24. Zhou Zhigang, Liu Zhili, Liu Xuexian.The research of the separation of spirulina maxim polysaccharide, purifying and anti-oxidation characteristics thereof.Botany Gazette, 1997,39 (1): 77～81
25. a left side continues far.The separation and purification and the bioactive preliminary study thereof of spirulina polysaccharide produced in Yunnan.Yunnan medicine, 1995,16 (6): 427～429
26.Filkwill?DL.Production，isolation，preliminarycharacterization?of?the?exopolysaccharide?of?CyanobacteriumSpirulina?platensis.Biochem?Lett，1993，15(6)：567～772
27. Chinese Academy of Sciences's Institute of Mathematics.Mathematical statistics method commonly used, 1974.
28. Zhang Longxiang chief editor.Biochemical test method and technology.Beijing: Higher Education Publishing House.1981, first version; 1997, second edition.
29. Wu Dongru chief editor.The biological chemistry of carbohydrate.Beijing: Higher Education Publishing House.1987.The 60-97 page or leaf
30.Stephen?H?and?Stephen?T.Solubility?of?inorganic?andorganic?compounds，Vol1，Part1，457～458
Claims (11)
1, spirulina polysaccharide extract is characterised in that and adopts the following steps preparation:
A, polysaccharide extract;
B, extracting solution are transferred the albumen iso-electric point, and be centrifugal then;
C, centrifugate are got supernatant concentration to finite concentration, adopt ethanol sedimentation, and insolubles is removed in centrifugal or filtration;
D, supernatant liquor carry out the secondary ethanol sedimentation, and be dry again, gets the spirulina polysaccharide extract.
2, the spirulina polysaccharide extract of claim 1, wherein polysaccharide extract to adopt that the lixiviate of diluted alkaline temperature is followed the example of, the lixiviate of aqueous solution temperature is followed the example of or the lixiviate of salts solution temperature is followed the example of and carried out.
3, the spirulina polysaccharide extract of claim 1, wherein the lixiviate of polysaccharide extraction employing aqueous solution temperature is followed the example of and is carried out.
4, the spirulina polysaccharide extract of claim 1, the extracting solution that wherein said extraction is doubly measured with 7-9, under 70-90 ℃ temperature about 7-10 hour.
5, the spirulina polysaccharide extract of claim 1, wherein said albumen iso-electric point transfer to 4-about 6.5, preferred about 5.0.
6, the spirulina polysaccharide extract of claim 1, wherein said centrifugal speed is between 3000-7500 rev/min.
7, the spirulina polysaccharide extract of claim 1, the alcohol precipitation concentration of wherein said polysaccharide is 75%.
8, the spirulina polysaccharide extract of claim 1, wherein said spissated multiple is between 2-6 times.
9, the spirulina polysaccharide extract of claim 1 wherein dryly carried out 5-9 hour under 60-70 ℃, certain vacuum tightness.
10, the purposes of spirulina polysaccharide in the preparation shengbai drug.
11, the purposes of spirulina polysaccharide in the medicine of preparation treatment tumour.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304428C (en) * | 2004-03-27 | 2007-03-14 | 中国科学院水生生物研究所 | Method for extracting polysaccharide by using blooms blue algae |
| CN1314711C (en) * | 2004-03-27 | 2007-05-09 | 中国科学院水生生物研究所 | Method for extracting Gexianmi amylose from nostoc |
| CN1322015C (en) * | 2004-03-27 | 2007-06-20 | 中国科学院水生生物研究所 | Method for extracting amylose of desert algae |
| CN102579477A (en) * | 2012-04-01 | 2012-07-18 | 新乡医学院 | Application of spirulina phatensis polysaccharide in preparing medicament for preventing and treating embryotoxicity caused by organophosphorus pesticide |
| CN102631366A (en) * | 2012-04-01 | 2012-08-15 | 新乡医学院 | Application of spirulina polysaccharides to preparation of drugs for preventing and treating male reproductive toxicity caused by organophosphorus pesticides (OPs) |
| CN103819577A (en) * | 2014-03-24 | 2014-05-28 | 福州大学 | Method for preparing spirulina platensis polysaccharide |
| CN105294870A (en) * | 2015-06-09 | 2016-02-03 | 深圳海王药业有限公司 | Spriulina polysacchride and preparation method thereof |
| CN107474103A (en) * | 2017-09-05 | 2017-12-15 | 皖西学院 | A kind of method of synchronous extraction hydriopsis cumingii water-solubility protein and polysaccharide |
-
2002
- 2002-08-01 CN CN 02125896 patent/CN1227267C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304428C (en) * | 2004-03-27 | 2007-03-14 | 中国科学院水生生物研究所 | Method for extracting polysaccharide by using blooms blue algae |
| CN1314711C (en) * | 2004-03-27 | 2007-05-09 | 中国科学院水生生物研究所 | Method for extracting Gexianmi amylose from nostoc |
| CN1322015C (en) * | 2004-03-27 | 2007-06-20 | 中国科学院水生生物研究所 | Method for extracting amylose of desert algae |
| CN102579477A (en) * | 2012-04-01 | 2012-07-18 | 新乡医学院 | Application of spirulina phatensis polysaccharide in preparing medicament for preventing and treating embryotoxicity caused by organophosphorus pesticide |
| CN102631366A (en) * | 2012-04-01 | 2012-08-15 | 新乡医学院 | Application of spirulina polysaccharides to preparation of drugs for preventing and treating male reproductive toxicity caused by organophosphorus pesticides (OPs) |
| CN102579477B (en) * | 2012-04-01 | 2013-04-03 | 新乡医学院 | Application of spirulina phatensis polysaccharide in preparing medicament for preventing and treating embryotoxicity caused by organophosphorus pesticide |
| CN102631366B (en) * | 2012-04-01 | 2013-06-05 | 新乡医学院 | Application of spirulina polysaccharides in preparation of drugs for preventing and treating male reproductive toxicity caused by organophosphorus pesticides (OPs) |
| CN103819577A (en) * | 2014-03-24 | 2014-05-28 | 福州大学 | Method for preparing spirulina platensis polysaccharide |
| CN103819577B (en) * | 2014-03-24 | 2016-05-04 | 福州大学 | A kind of preparation method of spirulina polysaccharide |
| CN105294870A (en) * | 2015-06-09 | 2016-02-03 | 深圳海王药业有限公司 | Spriulina polysacchride and preparation method thereof |
| CN105294870B (en) * | 2015-06-09 | 2017-07-25 | 深圳海王药业有限公司 | A kind of spirulina polysaccharide and preparation method thereof |
| CN107474103A (en) * | 2017-09-05 | 2017-12-15 | 皖西学院 | A kind of method of synchronous extraction hydriopsis cumingii water-solubility protein and polysaccharide |
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