CN102579477B - Application of spirulina phatensis polysaccharide in preparing medicament for preventing and treating embryotoxicity caused by organophosphorus pesticide - Google Patents
Application of spirulina phatensis polysaccharide in preparing medicament for preventing and treating embryotoxicity caused by organophosphorus pesticide Download PDFInfo
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- CN102579477B CN102579477B CN 201210097152 CN201210097152A CN102579477B CN 102579477 B CN102579477 B CN 102579477B CN 201210097152 CN201210097152 CN 201210097152 CN 201210097152 A CN201210097152 A CN 201210097152A CN 102579477 B CN102579477 B CN 102579477B
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Abstract
The invention relates to a novel medical application of spirulina phatensis polysaccharide, namely an effect of preventing and treating embryotoxicity caused by organophosphorus pesticide, and effects of inhibiting embryo survival rate reduction, embryonic mortality or embryo absorption rate increase and placenta weight reduction caused by organophosphorus pesticide. Pharmacological experiments prove that: the spirulina phatensis polysaccharide can inhibit survival number of pregnant rats, survival rate reduction and mortality or absorption rate increase and reduction of weight, length, tail length placenta weight of survival rats caused by organophosphorus pesticide (such as moncrotophos and phoxim), and can inhibit the embryotoxicity of the organophosphorus pesticide, and has good dosage dependence. The spirulina phatensis polysaccharide has excellent inhibition and protection effects to the embryotoxicity caused by the organophosphorus pesticide, can be used for preparing a medicament for preventing and treating the embryotoxicity caused by the organophosphorus pesticide, as well as preparing a medicament for preventing and treating diseases induced by embryotoxicity caused by the organophosphorus pesticide.
Description
Technical field
The present invention relates to the application of spirulina polysaccharide, be specifically related to spirulina polysaccharide and prevent with treatment because of the application in the medicine of caused by organophosphorus pesticide fetal toxicity disease in preparation.
Background technology
Organophosphorus pesticide (OPs) is that a class is efficient, the pesticide of low-residual, the wide spectrum pesticide that has become that China Today uses.The use of organophosphorus pesticide has been made distinctions won on the battlefield for the volume increase of farming, woods, fishery good harvest, but along with being widely used of organophosphorus pesticide, its residual phenomena in the foods such as vegetable and fruit is also comparatively general.Although most people has noted preventing the acute poisoning problem of organophosphorus pesticide, long-term, the low dosage of organophosphorus pesticide, Chronic exposure expose impact on the offspring to impact, the especially anemia of pregnant woman of human health, also do not cause people's enough attention.Epidemiological study is both at home and abroad found, suffer the area of organophosphorus pesticide pollution or the workman of insecticide factory for a long time, menstrual cycle of female changes, miscarriage increases, fetal intrauterine growth retardation, ovarian cancer and breast cancer incidence rising (Arbuckle TE, Lin Z, Mery LS, et al.An exploratory analysis of the effect of pesticide exposure on the risk of spontaneous abortion in an ontario farm population.Environ Health Perspect, 2001,109:851-857.).Research both domestic and external is found, organophosphorus pesticide has fetal toxicity, organophosphorus pesticide exposes, can cause pregnant mouse, body weight increases and reduces, the tire number of living reduces, stillborn fetus and absorption tire number increase, young for birth weight decline, hypoevolutism, deformity (Farag AT, Karkour TA, El-Okazy A.Developmental toxicity of orally administered technical dimethoate in rats.Birth Defects Res B Dev Reprod Toxicol, 2006,77:40-46.).Therefore inquire into the fetal toxicity mechanism of action of organophosphorus pesticide, seeks limited intervention medicine, China people's is healthy most important for protecting.
Spirulina polysaccharide (Spriulina polysacchrides) is to adopt the modern biochemistry technology, extracts from spirulina, contains pure natural biological product multiple and that biological activity is higher.Spirulina polysaccharide has significant inhibitory action to ascites hepatoma cells in the body; Spirulina polysaccharide has obvious protection effect to the damage of ionizing radiation, can also significantly alleviate the radiation genetic damage of bone marrow cells in mice; Spirulina polysaccharide reduces lipid peroxidation by suppressing free-radical oxidation reaction in the body, reduces the formation of organizing lipofuscin, can reach the effect that slows down body aging; Spirulina polysaccharide also has the proliferation of bone marrow cells of enhancing vigor, promotes DNA to repair anabolic effect good radiation-resisting functional is arranged and reduce radiotherapy, chemotherapy side effect; Spirulina polysaccharide can improve the nonspecific cellular immune function of body, and can promote body to turn different in nature humoral immune function, improves low ability of driving pathogenic bacteria, poisoning intrusion; Spirulina polysaccharide also can be used as the auxiliary treatment of hepatitis, diabetes, gastric ulcer.Spirulina polysaccharide is without any side effects, can long-term taking.
Summary of the invention
The object of the present invention is to provide a kind of new purposes of spirulina polysaccharide, i.e. application in preparation control caused by organophosphorus pesticide fetal toxicity disease medicament.The application of a kind of spirulina polysaccharide in the medicine for preparing inhibition embryo tire rate decline alive, embryo's still birth rate or the rising of absorption tire rate, placental weight decline also is provided simultaneously.
Medicine refers to take spirulina polysaccharide as main active under the present invention, and acceptable preparation in the medical treatment made from pharmaceutically suitable carrier is such as tablet, pill, capsule etc.The present invention finds by animal experiment study: take the effect that spirulina polysaccharide can reach the fetal toxicity of control caused by organophosphorus pesticide, play and suppress because of embryo's tire rate decline alive, embryo's still birth rate of caused by organophosphorus pesticide or the series of problems that absorption tire rate raises, placental weight descends.Concrete consumption is determined according to age of therapist, health, contact organophosphorus pesticide amount etc.
Spirulina polysaccharide of the present invention is the main active of spirulina, it has immunoregulatory activity, can play the effects such as antitumor, antioxidation, antiviral, antibacterium, ionizing radiation-resistant, reduction radiotherapy, chemotherapy side effect, and find not yet that at present it has any toxic and side effects.Spirulina polysaccharide can obtain by commercially available, also can extract by several different methods to obtain, and extracting method is ripe.
In organophosphorus pesticide, selecting highly toxic Azodrin and hypotoxic phoxim is representative, studied the fetal toxicity effect of organophosphorus pesticide, the fetal toxicity effect of finding organophosphorus pesticide mainly is to cause that pregnant Mus absorbs the tire rate and still birth rate raises, every nest is lived, and the tire number reduces; Live tire Mus body weight, height, tail is long and placental weight descends, thereby cause such as fetal birth deformity, miscarriage, stillborn fetus, embryo growth retardation, birth defect, a series of diseases such as infertile.The fetal toxicity effect of organophosphorus pesticide to mice that the present invention has passed through animal experimental observation, and spirulina polysaccharide is to the inhibitory action of organophosphorus pesticide fetal toxicity.Find after deliberation, spirulina polysaccharide can suppress the decline of body weight, body length, tail length and the placental weight of the pregnant Mus tire number alive of (such as Azodrin and phoxim) caused by organophosphorus pesticide, live tire rate decline and still birth rate, the rising of absorption tire rate and tire Mus, spirulina polysaccharide can suppress the fetal toxicity of organophosphorus pesticide, dosage is arranged preferably according to lazyness.
Beneficial effect of the present invention is: the invention provides the new medical use of spirulina polysaccharide, its fetal toxicity that organophosphorus pesticide is caused has good inhibition and protective effect.The pregnant Mus body weight that spirulina polysaccharide can suppress due to the organophosphorus pesticide (such as Azodrin, phoxim) increases minimizing, absorbs tire rate and still birth rate rising, the tire number minimizing alive of every nest, the tire Mus body weight of living, height, tail length and placental weight descend, and good dosage are arranged according to lazyness.The present invention has profound significance to the people of Long Term Contact organophosphorus pesticide especially anemia of pregnant woman, can play protection anemia of pregnant woman and filial generation thereof healthy, improve the quality of living, reduce the effects such as sterility and infertility, prenatal and postnatal care, life-saving.
The specific embodiment
Below further set forth new medical use and the beneficial effect thereof of spirulina polysaccharide among the present invention by test example, but be not used for limiting the scope of the invention.
Test example 1: the experimental study of fetal toxicity due to the spirulina polysaccharide inhibition Azodrin
1 materials and methods
1.1 material
Azodrin (40% water soluble preparation, Qingdao insecticide factory), spirulina polysaccharide (life institute of Chinese Marine University is so kind as to give).
1.2 laboratory animal and grouping
Select 120 of healthy SPF level sexual maturity kunming mices, body weight is at (25 ± 2) g, and is female: hero is 2: 1, available from Xinxiang College of Medical Science zoopery center.Adaptability is raised 7d, room temperature (25 ± 2) ℃, humidity 50%-55%.
With mice by female: hero is to mate copulation at 2: 1, checks that female Mus has the depletion of YIN bolt morning next day, and the female Mus of depletion of YIN bolt is got vaginal secretions with the cotton swab that soaks normal saline and is applied on the microscope slide that contains a normal saline, checks to have or not sperm under low power lens.Be decided to be the 0th day pregnancy period the same day of finding cloudy bolt or sperm.The female Mus that will inseminate is divided into 6 groups at random, 15 every group.Be respectively dosage protection group (E), spirulina polysaccharide high dose protection group (E) in Normal group (A), spirulina polysaccharide matched group (B), Azodrin matched group (C), spirulina polysaccharide low dosage protection group (D), the spirulina polysaccharide.Wherein not administration of matched group (A); give distilled water; the spirulina polysaccharide matched group (B) give spirulina polysaccharide 200mg/kg; the Azodrin matched group (C) give 0.20mg/kg; spirulina polysaccharide low dosage protection group (D) gives spirulina polysaccharide 100mg/kg+ Azodrin 0.20mg/kg; dosage protection group (E) gives spirulina polysaccharide 200mg/kg+ Azodrin 0.20mg/kg in the spirulina polysaccharide, and spirulina polysaccharide high dose protection group (F) gives spirulina polysaccharide 400mg/kg+ Azodrin 0.20mg/kg.And guaranteeing sufficient drinking-water and supply of forage, the insemination Mus was weighed the same day.Adopt the gavage mode to contaminate in the pregnancy period, poisoning dosage is 10ml/kg, every day 1 time.
1.3 statistical method
Adopt SPSS 10.0 softwares to carry out statistical analysis.The measurement data experimental data all represents with x ± s, relatively adopts homogeneity test of variance and one factor analysis of variance (One Way ANOVA) between many groups.When comparing in twos between further organizing, if when variance is neat, adopt the SNK check; If during heterogeneity of variance, adopt the Games-Howell check.The enumeration data experimental data all represents with rate, relatively adopts in twos χ between group
2Check.Take P＜0.05 as difference statistical significance is arranged.
2 methods and result
In the 20th day pregnancy period, put to death pregnant Mus, cut the abdominal cavity open and take out the uterus, record uterus weight, tire number alive, stillborn fetus number (containing early stage and late fetal death) and absorb the tire number.Get tire Mus and Placenta Hominis, claim live tire Mus body weight and Placental Weight, the measurement body is long, tail is long.The result as shown in Tables 1 and 2.
The protective effect (n=15) that table 1 spirulina polysaccharide raises to pregnant Mus uterus weight due to the Azodrin and the tire number of living, live tire rate decline and still birth rate, absorption tire rate
Annotate: compare with the A group: * P＜0.05, compare with the C group: △ P＜0.05.
Table 1 demonstration, (A) compares with Normal group, and Azodrin matched group (C) tire number alive and the tire rate of living all descend, and still birth rate and absorption tire rate all raise, and difference has statistical significance (P＜0.05).The spirulina polysaccharide protection group can suppress pregnant Mus tire number alive, tire rate decline alive and still birth rate, the rising of absorption tire rate that the Azodrin contamination causes; the most remarkable with spirulina polysaccharide high dose protection group (F) effect; spirulina polysaccharide high dose protection group (F) tire number alive, the tire rate of living and still birth rate, absorption tire rate; compare with Azodrin matched group (C) that there were significant differences (P＜0.05); (A) compares with Normal group, do not have significant difference (P＞0.05).
Table 2 spirulina polysaccharide is to tire Mus body weight due to the Azodrin, body is long, tail is long, placental weight descends protective effect (n=15)
Annotate: compare with the A group: * P＜0.05, compare with the C group: △ P＜0.05.
As seen from Table 2, (A) compares with Normal group, and the body weight of Azodrin phosphorus matched group (C) tire Mus, body length, tail length and placental weight all descend, and difference has statistical significance (P＜0.05).The spirulina polysaccharide protection group can suppress that body weight, the body of the tire Mus that Azodrin contamination causes is long, tail is long and placental weight descends; the most remarkable with spirulina polysaccharide high dose protection group (F) effect; the body weight of spirulina polysaccharide high dose protection group (F) tire Mus, body length, tail length and placental weight; compare with Azodrin matched group (C) that there were significant differences (P＜0.05); (A) compares with Normal group, do not have significant difference (P＞0.05).
Test example 2: the experimental study of fetal toxicity due to the spirulina polysaccharide inhibition phoxim
1 materials and methods
1.1 material
Phoxim (40% cream, Shandong Dongtai Agrochemical Co., Ltd), spirulina polysaccharide (life institute of Chinese Marine University is so kind as to give).
1.2 laboratory animal and grouping
Select 120 of healthy SPF level sexual maturity kunming mices, body weight is between (25 ± 2) g, and is female: hero is 2: 1, available from Xinxiang College of Medical Science zoopery center.Adaptability is raised 7d, room temperature (25 ± 2) ℃, humidity 50%-55%.
With mice by female: hero is to mate copulation at 2: 1, checks that female Mus has the depletion of YIN bolt morning next day, and the female Mus of depletion of YIN bolt is got vaginal secretions with the cotton swab that soaks normal saline and is applied on the microscope slide that contains a normal saline, checks to have or not sperm under low power lens.Be decided to be the 0th day pregnancy period the same day of finding cloudy bolt or sperm.The female Mus that will inseminate is divided into 6 groups at random, 15 every group.Be respectively dosage protection group (E) in Normal group (A), spirulina polysaccharide matched group (B), phoxim matched group (C), spirulina polysaccharide low dosage protection group (D), the spirulina polysaccharide.Wherein not administration of matched group (A); give distilled water; the spirulina polysaccharide matched group (B) give spirulina polysaccharide 200mg/kg; the phoxim matched group (C) give 20mg/kg; spirulina polysaccharide low dosage protection group (D) gives spirulina polysaccharide 100mg/kg+ phoxim 20mg/kg; dosage protection group (E) gives spirulina polysaccharide 200mg/kg+ phoxim 20mg/kg in the spirulina polysaccharide, and spirulina polysaccharide high dose protection group (F) gives spirulina polysaccharide 400mg/kg+ phoxim 20mg/kg.And guaranteeing sufficient drinking-water and supply of forage, the insemination Mus was weighed the same day.Adopt the gavage mode to contaminate in the pregnancy period, poisoning dosage is 10ml/kg, every day 1 time.
1.3 statistical method
Adopt SPSS 10.0 softwares to carry out statistical analysis.The measurement data experimental data all represents with x ± s, relatively adopts homogeneity test of variance and one factor analysis of variance (One Way ANOVA) between many groups.When comparing in twos between further organizing, if when variance is neat, adopt the SNK check; If during heterogeneity of variance, adopt the Games-Howell check.The enumeration data experimental data all represents with rate, relatively adopts in twos χ between group
2Check.Take P＜0.05 as difference statistical significance is arranged.
2 methods and result
In the 20th day pregnancy period, put to death pregnant Mus, cut the abdominal cavity open and take out the uterus, record uterus weight, tire number alive, stillborn fetus number (containing early stage and late fetal death) and absorb the tire number.Get tire Mus and Placenta Hominis, claim live tire Mus body weight and Placental Weight, the measurement body is long, tail is long.The result is shown in table 3 and table 4.
Table 3 spirulina polysaccharide is to pregnant Mus uterus weight due to the phoxim and the tire number of living, live tire rate decline and still birth rate, absorption tire rate height protective effect (n=15)
Annotate: compare with the A group: * P＜0.05, compare with the C group: △ P＜0.05.
Table 3 demonstration, (A) compares with Normal group, and phoxim matched group (C) tire number alive and the tire rate of living all descend, and still birth rate and absorption tire rate all raise, and difference has statistical significance (P＜0.05).The spirulina polysaccharide protection group can suppress pregnant Mus tire number alive, tire rate decline alive and still birth rate, the rising of absorption tire rate that the phoxim contamination causes; the most remarkable with spirulina polysaccharide high dose protection group (F) effect; spirulina polysaccharide high dose protection group (F) tire number alive, the tire rate of living and still birth rate, absorption tire rate; compare with phoxim matched group (C) that there were significant differences (P＜0.05); (A) compares with Normal group, do not have significant difference (P＞0.05).
Table 4 spirulina polysaccharide is to tire Mus body weight due to the phoxim, body is long, tail is long, placental weight descends protective effect (n=15)
Annotate: compare with the A group: * P＜0.05, compare with the C group: △ P＜0.05.
As seen from Table 4, (A) compares with Normal group, and the body weight of phoxim matched group (C) tire Mus, body length, tail length and placental weight all descend, and difference has statistical significance (P＜0.05).The spirulina polysaccharide protection group can suppress that body weight, the body of the tire Mus that phoxim contamination causes is long, tail is long and placental weight descends; the most remarkable with spirulina polysaccharide high dose protection group (F) effect; the body weight of spirulina polysaccharide high dose protection group (F) tire Mus, body length, tail length and placental weight; compare with phoxim matched group (C) that there were significant differences (P＜0.05); (A) compares with Normal group, do not have significant difference (P＞0.05).
Can find out from above each experimental example, spirulina polysaccharide can suppress the decline of body weight, body length, tail length and the placental weight of the pregnant Mus tire number alive of (such as Azodrin and phoxim) caused by organophosphorus pesticide, live tire rate decline and still birth rate, the rising of absorption tire rate and tire Mus, spirulina polysaccharide can suppress the fetal toxicity of organophosphorus pesticide, dosage is arranged preferably according to lazyness.Fetal toxicity has good inhibition and protective effect to spirulina polysaccharide to caused by organophosphorus pesticide; can be used for preparing the medicine of control caused by organophosphorus pesticide fetal toxicity, the medicine that also can be used for preparing prevention and treat the disease of bringing out because of the caused by organophosphorus pesticide fetal toxicity.
Claims (6)
1. the application of spirulina polysaccharide in the medicine of preparation prevention caused by organophosphorus pesticide fetal toxicity disease, described fetal toxicity disease is stillborn fetus or absorbs tire.
2. application according to claim 1 is characterized in that: described organophosphorus pesticide is Azodrin, phoxim, paraoxon or triazophos.
3. the application of spirulina polysaccharide in the medicine that preparation inhibition caused by organophosphorus pesticide embryo tire rate alive descends.
4. the application of spirulina polysaccharide in the medicine of preparation inhibition caused by organophosphorus pesticide embryo's still birth rate or the rising of absorption tire rate.
5. the application of spirulina polysaccharide in the medicine that preparation inhibition caused by organophosphorus pesticide placental weight descends.
6. according to claim 3-5 each described application is characterized in that: described organophosphorus pesticide is Azodrin, phoxim, paraoxon or triazophos.
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| CN103041368B (en) * | 2013-01-18 | 2014-04-02 | 新乡医学院 | Application of phycocyanin in preparation of drug for preventing and treating embryotoxicity caused by organophosphorus pesticide |
| CN108546683B (en) * | 2018-03-23 | 2021-09-21 | 广西壮族自治区水牛研究所 | Method for efficiently producing transgenic buffalo embryos by adenovirus mediation |
| CN108478594B (en) * | 2018-04-13 | 2020-07-24 | 桂林医学院 | Application of fucoidin in preparation of medicine for preventing and treating embryotoxicity caused by organophosphorus pesticide |
| CN116196315A (en) * | 2022-12-09 | 2023-06-02 | 首都医科大学附属北京口腔医院 | Application of VO-Ohpic in preparation of product related to reversing embryonic cleft palate |
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