CN1385217A - Chitosan medical nerve and productive method thereof - Google Patents
Chitosan medical nerve and productive method thereof Download PDFInfo
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- CN1385217A CN1385217A CN 02113103 CN02113103A CN1385217A CN 1385217 A CN1385217 A CN 1385217A CN 02113103 CN02113103 CN 02113103 CN 02113103 A CN02113103 A CN 02113103A CN 1385217 A CN1385217 A CN 1385217A
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Abstract
The present invention discloses a kind of chitosan medical nerve, including chitosan bio-catheter and chitosan fibre scaffold inserted into the chitosan bio-cathether. Its production method includes the preparation of the above-mentioned materials and their combination steps. It is a pure natural bio-degradable material, so that it possesses good bio-compatibility, has no any adverse reaction. It the course of preparation is does not add any foaming agent, cross-linking agent and surfactant, therefore said product contains no any exogeneous toxic substances capable of producing said effect.
Description
Technical field:
The present invention relates to a kind of medical nerve that is used for the damaged nerve of bridge joint.
Background technology:
Peripheral nerve defection is a difficult problem of orthopaedics, microsurgery, the traumatology department, war-surgery's treatment, though modern microsurgical technique is highly accurate, the functional rehabilitation rate after peripheral nerve nerve injury is at present repaired is still unsatisfactory.Chinese patent literature CN1319379A discloses a kind of medical artificial nerve graft and production method, but it is owing to contain compositions such as foaming agent, cross-linking agent, surfactant, and product has certain side effect.
Summary of the invention:
The object of the present invention is to provide a kind of chitosan medical nerve and production method that does not contain any exogenous toxicity, side effect material.
Technical solution of the present invention is:
A kind of chitosan medical nerve, its feature: comprise the chitosan biological duct, be embedded with the chitin fiber support in the chitosan biological duct.
The diameter of medical nerve is 2.0～4.0mm, and length is 3.0～6.0mm.
The production method of chitosan medical nerve, its feature: comprise the following steps:
1. prepare the chitosan biological duct: chitosan is dissolved in weak acid solution, then solution is passed through the tubulose mould, and molding in alkaline solidification liquid, after the molding conduit is soaked in water;
2. prepare the chitin fiber support: chitosan is dissolved, then solution is processed into fiber by wet spinning technology in weak acid solution;
3. combination: the chitin fiber support that makes is embedded in the chitosan catheter.
Step 1. in, the deacetylation of chitosan is 85～90%.Step 1. in, weak acid is acetic acid or phosphoric acid, citric acid, lactic acid, concentration is 2～15%.Step 1. in, alkaline solidification liquid is the solution that contains sodium hydroxide 8～20% or potassium hydroxide 8～20% or sodium carbonate 8～20%.
Step 1. in, alkaline solidification liquid is the solution that contains sodium hydroxide 8～20% or potassium hydroxide 8～20% or sodium carbonate 8～20%, contains ethanol 5～30% simultaneously.
Step 2. in, the deacetylation of chitosan is 85～90%, weak acid is acetic acid or phosphoric acid, citric acid, lactic acid, weak acid concentration is 2～15%.
Product material therefor of the present invention is a pure chitosan, and it is the natural biological degradable materials, with human body excellent biological compatibility is arranged, and does not have any untoward reaction; Because in preparation process, do not add any foaming agent, cross-linking agent, surfactant, therefore the product that makes does not contain the material of any exogenous toxicity, side effect, and the intensity of conduit, good springiness; The tube wall of conduit is a chitosan film, and it is natural semipermeable membrane, and therefore the conveying for the required nutrient substance of nerve growth process provides essential approach; Adopt chitosan stent, make product have certain mechanical strength and toughness, for the growth of neurocyte provides unimpeded space, simultaneously for instructing the neurocyte ordering growth that necessary inducing action is provided.
The specific embodiment:
Embodiment 1:
(1) preparation of chitosan conduit: with deacetylation is that chitosan 5 gram that 85-90% (example 85%, 88%, 90%) has a certain viscosity is dissolved in 50-150ml (routine 50ml, 100ml, the 150ml) weak acid solution, used weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), and the concentration of acid is 2%-15% (example: 2%, 7%, 12%, 15%).After treating that chitosan fully dissolves, leave standstill about 3 hours.Then solution is passed through the tubulose mould, at alkaline solidification liquid---molding in the solidification liquid of sodium hydroxide, carry out processing and forming.Used solidification liquid contains ethanol 5%-30% (example: solution 5%, 15%, 25%, 30%) simultaneously for containing sodium hydroxide 8%-20% (example 8%, 13%, 17%, 20%).After the molding conduit is fully soaked in distilled water, to remove sodium acetate, sodium phosphate or the sodium lactate inorganic salt in the chitosan.
(2) preparation of chitin fiber support: with deacetylation is that chitosan 2 gram that 85-90% (example 85%, 88%, 90%) has a certain viscosity is dissolved in the 100ml weakly acidic solution, used weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), and the concentration of acid is 2%-15% (example: 2%, 7%, 12%, 15%).After treating that chitosan fully dissolves, left standstill 3 hours.Then solution is processed into fiber by conventional wet spinning technology.
(3) assembling of medical nerve: the fibrous framework of making is embedded conduit.Make diameter respectively for 2-4mm length is medical people's nerve of 3-6cm according to purposes, be applicable to the reparation of the thickness nerve of humans and animals.
The neural cobalt-60 of using of the medical artificial that assembles is shone, carry out sterilization, package spare.
Embodiment 2:
The sodium hydroxide solidification liquid adopts the solution that contains sodium hydroxide 8～20% (example 8%, 13%, 17%, 20%).All the other are with embodiment 1.
Embodiment 3:
The alkalescence solidification liquid contains ethanol 5%-30% (example: solution 5%, 15%, 25%, 30%) simultaneously for containing potassium hydroxide 8%-20% (example 8%, 13%, 17%, 20%).All the other are with embodiment 1.
Embodiment 4:
The alkalescence solidification liquid adopts the solution that contains potassium hydroxide 8～20% (example 8%, 13%, 17%, 20%).All the other are with embodiment 2.
Embodiment 5:
The alkalescence solidification liquid contains ethanol 5%-30% (example: solution 5%, 15%, 25%, 30%) simultaneously for containing sodium carbonate 8%-20% (example 8%, 13%, 17%, 20%).All the other are with embodiment 1.
Embodiment 6:
The alkalescence solidification liquid adopts the solution that contains sodium carbonate 8～20% (example 8%, 13%, 17%, 20%).All the other are with embodiment 2.
Claims (8)
1, a kind of chitosan medical nerve is characterized in that: comprise the chitosan biological duct, be embedded with the chitin fiber support in the chitosan biological duct.
2, chitosan medical nerve according to claim 1 is characterized in that: the diameter of medical nerve is 2.0～4.0mm, and length is 3.0～6.0cm.
3, a kind of production method of chitosan medical nerve is characterized in that: comprise the following steps:
1. prepare the chitosan biological duct: chitosan is dissolved in weak acid solution, then solution is passed through the tubulose mould, and molding in alkaline solidification liquid, after the molding conduit is soaked in water;
2. prepare the chitin fiber support: chitosan is dissolved, then solution is processed into fiber by wet spinning technology in weak acid solution;
3. combination: the chitin fiber support that makes is embedded in the chitosan catheter.
4, the production method of chitosan medical nerve according to claim 3 is characterized in that: step 1. in, the deacetylation of chitosan is 85～90%.
5, the production method of chitosan medical nerve according to claim 3 is characterized in that: step 1. in, weak acid is acetic acid or phosphoric acid, citric acid, lactic acid, concentration is 2～15%.
6, the production method of chitosan medical nerve according to claim 3 is characterized in that: step 1. in, alkaline solidification liquid is the solution that contains sodium hydroxide 8～20% or potassium hydroxide 8～20% or sodium carbonate 8～20%.
7, the production method of chitosan medical nerve according to claim 3 is characterized in that: step 1. in, alkaline solidification liquid is the solution that contains sodium hydroxide 8～20% or potassium hydroxide 8～20% or sodium carbonate 8～20%, contains ethanol 5～30% simultaneously.
8, the production method of chitosan medical nerve according to claim 3 is characterized in that: step 2. in, the deacetylation of chitosan is 85～90%, weak acid is acetic acid or phosphoric acid, citric acid, lactic acid, weak acid concentration is 2～15%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021131031A CN1168505C (en) | 2002-05-30 | 2002-05-30 | Chitosan medical nerve and productive method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021131031A CN1168505C (en) | 2002-05-30 | 2002-05-30 | Chitosan medical nerve and productive method thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN1385217A true CN1385217A (en) | 2002-12-18 |
| CN1168505C CN1168505C (en) | 2004-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB021131031A Expired - Lifetime CN1168505C (en) | 2002-05-30 | 2002-05-30 | Chitosan medical nerve and productive method thereof |
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| CN (1) | CN1168505C (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1298298C (en) * | 2004-05-14 | 2007-02-07 | 清华大学 | Preparation method of chitosan tubular bracket |
| WO2007042281A2 (en) | 2005-10-12 | 2007-04-19 | Thomas Freier | Processing of chitosan and chitosan derivatives |
| CN101732760A (en) * | 2009-12-31 | 2010-06-16 | 扬州大学 | Chitosan non-vessel stent and preparation method thereof |
| CN102343114A (en) * | 2011-10-24 | 2012-02-08 | 南通大学 | Tissue engineering nerve graft and application thereof |
| CN102343112A (en) * | 2011-10-09 | 2012-02-08 | 江苏益通生物科技有限公司 | Chitosan artificial nerve graft containing neurotrophic factor and preparation method thereof |
| CN102888672A (en) * | 2011-07-20 | 2013-01-23 | 李永兴 | Method for preparing chitosan fibers for textile industry from high-concentration solution prepared from composite solvent |
| CN106421899A (en) * | 2016-12-02 | 2017-02-22 | 上海其胜生物制剂有限公司 | Method for preparing peripheral nerve conduit by salt-induced phase transition |
| CN107469144A (en) * | 2017-07-29 | 2017-12-15 | 青岛慧生惠众生物科技有限公司 | A kind of chitosan-based composite nerve conduit and its preparation method and application |
| CN109498849A (en) * | 2018-06-25 | 2019-03-22 | 北京百利康生化有限公司 | A kind of medical tubal branch rack device |
| CN111450318A (en) * | 2020-03-26 | 2020-07-28 | 南通大学 | Chitosan nerve conduit with illumination bridged nerve tissue and preparation method and application thereof |
| CN112323181A (en) * | 2020-11-23 | 2021-02-05 | 江南大学 | Preparation method of high-performance chitosan fiber |
| CN115607732A (en) * | 2022-10-18 | 2023-01-17 | 尧舜泽生物医药(南京)有限公司 | Bionic nerve graft and preparation method thereof |
-
2002
- 2002-05-30 CN CNB021131031A patent/CN1168505C/en not_active Expired - Lifetime
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1298298C (en) * | 2004-05-14 | 2007-02-07 | 清华大学 | Preparation method of chitosan tubular bracket |
| WO2007042281A2 (en) | 2005-10-12 | 2007-04-19 | Thomas Freier | Processing of chitosan and chitosan derivatives |
| US8414925B2 (en) | 2005-10-12 | 2013-04-09 | Thomas Freier | Processing of acylchitosan hydrogels |
| US9034379B2 (en) | 2005-10-12 | 2015-05-19 | Thomas Freier | Processing of acylchitosan hydrogels |
| CN101732760A (en) * | 2009-12-31 | 2010-06-16 | 扬州大学 | Chitosan non-vessel stent and preparation method thereof |
| CN102888672A (en) * | 2011-07-20 | 2013-01-23 | 李永兴 | Method for preparing chitosan fibers for textile industry from high-concentration solution prepared from composite solvent |
| CN102343112A (en) * | 2011-10-09 | 2012-02-08 | 江苏益通生物科技有限公司 | Chitosan artificial nerve graft containing neurotrophic factor and preparation method thereof |
| CN102343114A (en) * | 2011-10-24 | 2012-02-08 | 南通大学 | Tissue engineering nerve graft and application thereof |
| CN106421899B (en) * | 2016-12-02 | 2019-07-05 | 上海其胜生物制剂有限公司 | A kind of method that salt-induced phase transformation prepares peripheral nerve conduit |
| CN106421899A (en) * | 2016-12-02 | 2017-02-22 | 上海其胜生物制剂有限公司 | Method for preparing peripheral nerve conduit by salt-induced phase transition |
| CN107469144A (en) * | 2017-07-29 | 2017-12-15 | 青岛慧生惠众生物科技有限公司 | A kind of chitosan-based composite nerve conduit and its preparation method and application |
| CN109498849A (en) * | 2018-06-25 | 2019-03-22 | 北京百利康生化有限公司 | A kind of medical tubal branch rack device |
| CN109498849B (en) * | 2018-06-25 | 2020-04-24 | 北京百利康生化有限公司 | Medical fallopian tube support device |
| CN111450318A (en) * | 2020-03-26 | 2020-07-28 | 南通大学 | Chitosan nerve conduit with illumination bridged nerve tissue and preparation method and application thereof |
| CN112323181A (en) * | 2020-11-23 | 2021-02-05 | 江南大学 | Preparation method of high-performance chitosan fiber |
| CN112323181B (en) * | 2020-11-23 | 2022-03-04 | 江南大学 | Preparation method of high-performance chitosan fiber |
| CN115607732A (en) * | 2022-10-18 | 2023-01-17 | 尧舜泽生物医药(南京)有限公司 | Bionic nerve graft and preparation method thereof |
| WO2023179045A1 (en) * | 2022-10-18 | 2023-09-28 | 南通大学 | Bionic nerve graft and preparation method therefor |
| CN115607732B (en) * | 2022-10-18 | 2023-10-20 | 尧舜泽生物医药(南京)有限公司 | Bionic nerve graft and preparation method thereof |
| JP7560189B2 (en) | 2022-10-18 | 2024-10-02 | 南通大学 | Biomimetic nerve graft and method for preparing same |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1168505C (en) | 2004-09-29 |
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Granted publication date: 20040929 |