CN100484496C - Method for preparing chitin-containing medical nerve graft - Google Patents
Method for preparing chitin-containing medical nerve graft Download PDFInfo
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- CN100484496C CN100484496C CNB2005100391928A CN200510039192A CN100484496C CN 100484496 C CN100484496 C CN 100484496C CN B2005100391928 A CNB2005100391928 A CN B2005100391928A CN 200510039192 A CN200510039192 A CN 200510039192A CN 100484496 C CN100484496 C CN 100484496C
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Abstract
A medical nerve-grafting object containing chitin is prepared through preparing the cathether containing chitin from chitosan, preparing the chitin fibre supporter from chitosan, and embedding said supporter in said cathether. Its advantages are high effect, high and controllable degradability and high biocompatibility.
Description
Technical field:
The present invention relates to a kind of medical nerve that is used for the damaged nerve of bridge joint.
Background technology:
The peripheral nerve defection that (or operation) causes because wound is comparatively common clinically, but the neurologic defect of central long distance just has to rely on graft to come the bridge joint reparation can not rely on end-to-end stitching to remedy neural disappearance the time.Though sought and developed the history in existing more than 100 year of comparatively suitable graft problem, but except autologous nerve becomes first-selected neurologic defect bridge joint graft, do not find comparatively desirable yet and recognized altogether and the neural transplantation substitute of extensive use clinically the mankind itself so far.In nerve autograft,, also fail to be extensive use of clinically because, organizational structure limited for the nerve source of transplanting usefulness and size are difficult to coupling, transplant for reasons such as head of district's phase denervation.
Along with the appearance and the development of tissue engineering, provide a new outlet for making up the nerve autograft succedaneum.Nearly two during the last ten years, and people try out in the Biodegradable material of peripheral nerve tissue's engineering and are generally some high molecular polymers, comprises their copolymer and the about kind more than ten of derivant; Also all exist different separately shortcomings.
Chitin is the polymer of acetylglucosamine, is the abundant polysaccharide of reserves in the nature, is only second to cellulose.But chitin solubility property extreme difference is insoluble to the diluted acid diluted alkaline, only is dissolved in the solution of dichloromethane and lithium chloride, and this has limited the processing and forming and the application of chitin greatly.Chitosan is that chitin makes through the pyritous deacetylation of concentrated base.When the chitin deacetylase degree greater than 70% the time, just can be dissolved in dilute acid soln, we just are referred to as chitosan.Chitin is preparing by deacetylation in the process of chitosan, and the protein that coexists with chitin also is eliminated totally.Protein content will be far smaller than chitin in the chitosan.The Chinese invention patent ZL02113103.1 that we obtain has before this invented chitosan medical nerve, and from entering the situation of clinic trial, chitosan is a kind of comparatively ideal tissue engineering material.But nervous tissue's engineering conduit degradation speed in vivo of simple Preparation of Chitosan is undesirable, and uncontrollable, and this has just limited its extensive use.Chitin since (1) at present chitin all adopt the diluted acid desalination, diluted alkaline removes albumen, thus the impurity in the chitin especially protein content is higher, be used for the people and know from experience and cause serious inflammatory reaction; (2) the chitin solubility property is poor, only is dissolved in the organic solvent of some halogenated hydrocarbons, therefore is difficult to carry out processing and forming; (3) degree of acetylation of chitin is difficult to reasons such as variation, has limited its application in this respect.Therefore up to the present, yet there are no the artificial nerve graft of chitin preparation.
Summary of the invention:
The object of the present invention is to provide a kind of result of use good, degradation property is good, with human body the medical nerve graft that contains chitin and the preparation method of excellent biological compatibility is arranged.
Technical solution of the present invention is:
A kind of medical nerve graft that contains chitin is characterized in that: make through the following step: the conduit that 1. Preparation of Chitosan is become to contain chitin;
2. Preparation of Chitosan is become the chitin fiber support;
3. combination: the chitin fiber support embedding that makes is contained in the conduit of chitin.
A kind of preparation method that contains the medical nerve graft of chitin is characterized in that: comprise the following steps:
1. Preparation of Chitosan is become to contain the conduit of chitin;
2. Preparation of Chitosan is become the chitin fiber support;
3. combination: the chitin fiber support embedding that makes is contained in the conduit of chitin.
Step becomes the concrete grammar of chitin fiber support to be Preparation of Chitosan in 2.: chitosan is dissolved in weak acid solution, then solution is processed into chitin fiber by wet spinning technology; Again the chitin fiber that obtains is carried out acetylization reaction, obtaining degree of acetylation is the 30-100% chitin fiber.
The concrete grammar that step becomes to contain the conduit of chitin with Preparation of Chitosan in 1. is: chitosan is dissolved with weak acid, then with solution by the tubulose mould, and molding in alkaline solidification liquid after the molding is cleaned the conduit water, drying gets chitosan catheter; Again the chitosan catheter that obtains is carried out acetylization reaction, obtaining degree of acetylation is 30-100% chitin conduit.
The concrete grammar that step becomes to contain the conduit of chitin with Preparation of Chitosan in 1. is: earlier chitosan is made powder, then the chitosan powder is carried out acetylization reaction, obtaining degree of acetylation is 30-100% chitin powder; Other gets chitosan be dissolved into solution with weak acid after, add the above-mentioned chitin powder that obtains, mix homogeneously, then with mixture by the tubulose mould, and molding in alkaline solidification liquid after the molding is cleaned the conduit water, drying must contain the conduit of chitin.
Acetylization reaction is to carry out in the alcoholic solution of acetic anhydride, and reaction temperature is 0～60 ℃, and the response time is 0.5～6 hour, also through washing, and puts into sodium hydroxide or potassium hydroxide solution is handled behind the acetylization reaction.
In the alcoholic solution of acetic anhydride, the concentration of acetic anhydride is 0.1～50%, and alcoholic solution is methanol or ethanol.When chitin powder adding chitosan solution was prepared conduit, the consumption of chitin was 10～90% of chitosan and a chitin amount sum.Weak acid is acetic acid or phosphoric acid, citric acid, lactic acid, and concentration is 2～15%.The alkalescence solidification liquid is the solution that contains sodium hydroxide 8～20% or potassium hydroxide 8～20% or sodium carbonate 8～20%.
Product all material of the present invention is high purity of shell polysaccharide and the high-purity crust that makes by acetylation with the high purity of shell polysaccharide, and they are the natural degradable material, with human body excellent biological compatibility are arranged.The product that makes does not contain because the exogenous toxicity, side effect material that preparation technology brings into.Duct wall has three structures only of enriching micropore, and for the conveying of the required nutrient substance of nerve growth process provides necessary approach, result of use is good.Adopt chitin fiber support in the conduit, for the growth of neurocyte provides necessary inducing action and necessary growing space with suitable degradation speed.
The invention will be further described below in conjunction with embodiment.
The specific embodiment:
Embodiment 1:
1. Preparation of Chitosan is become to contain the conduit of chitin: chitosan is dissolved with weak acid, weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), concentration is 2～15% (examples 2%, 8%, 15%), then solution is passed through the tubulose mould, and molding in alkaline solidification liquid, the alkalescence solidification liquid is solution (or potassium hydroxide 8～20% or the sodium carbonate 8～20% that contains sodium hydroxide 8～20%, also can contain ethanol 5～30% simultaneously), after the molding conduit water is cleaned the dry chitosan catheter that gets; The alcoholic solution of the chitosan catheter that obtains being put into acetic anhydride carries out acetylization reaction again; reaction temperature is 0～60 ℃ (0 ℃, 30 ℃, 60 ℃ of examples); response time is 0.5～6 hour (example 0.5 hour, 2 hours, 4 hours, 6 hours); (the acetyl degree of the time of reaction and temperature effect chitin; response time is long, reaction temperature is high, the acetyl degree height of chitin then, otherwise; then the acetyl degree is low, can obtain the chitin of different acetyl degree as required).In the alcoholic solution of acetic anhydride, the concentration of acetic anhydride is 0.1～50% (example 0.1%, 10%, 30%, 50%), and alcoholic solution is methanol (or ethanol).Water cleans behind the acetylization reaction, puts into the sodium hydroxide of 1mol/L or potassium hydroxide solution then 24 hours, and water cleans up, and drying obtains the chitin conduit that degree of acetylation is 30-100%.
2. Preparation of Chitosan is become the chitin fiber support: chitosan is dissolved in weak acid solution, weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), concentration is 2～15% (examples 2%, 8%, 15%), then solution is processed into chitin fiber by wet spinning technology; The alcoholic solution of the chitin fiber that obtains being put into acetic anhydride carries out acetylization reaction again, and reaction temperature is 0～60 ℃ (0 ℃, 30 ℃, 60 ℃ of examples), and the response time is 0.5～6 hour (example 0.5 hour, 2 hours, 4 hours, 6 hours).In the alcoholic solution of acetic anhydride, the concentration of acetic anhydride is 1～50% (example 1%, 20%, 40%, 50%), and alcoholic solution is methanol (or ethanol).Water cleans behind the acetylization reaction, puts into the sodium hydroxide of 1mol/L or potassium hydroxide solution then 24 hours, and water cleans up, and drying obtains the chitin fiber that degree of acetylation is 30-100%.
3. combination: the chitin fiber support that makes is embedded in the chitin conduit, obtain the chitin medical nerve graft.
Embodiment 2:
1. earlier chitosan is made powder by machining or spray drying; the alcoholic solution of the chitosan powder being put into acetic anhydride carries out acetylization reaction then; reaction temperature is 0～60 ℃ (0 ℃, 30 ℃, 60 ℃ of examples), and the response time is 0.5～6 hour (example 0.5 hour, 2 hours, 4 hours, 6 hours).In the alcoholic solution of acetic anhydride, the concentration of acetic anhydride is 0.1～50% (example 0.1%, 20%, 40%, 50%), and alcoholic solution is methanol (or ethanol).Water cleans behind the acetylization reaction, puts into the sodium hydroxide of 1mol/L or potassium hydroxide solution then 24 hours, and water cleans up, and it is 30-100% chitin powder that drying obtains degree of acetylation; Other gets chitosan be dissolved into solution with weak acid after, add the above-mentioned chitin powder that obtains, weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), concentration is 2～15% (examples 2%, 8%, 15%), the addition of chitin powder is that 10～90% of chitosan and chitin amount sum (can be 10%, 50%, 90%), mix homogeneously, then mixture is passed through the tubulose mould, and molding in alkaline solidification liquid, the alkalescence solidification liquid is identical with embodiment 1, after the molding conduit water is cleaned the dry conduit (being the chitin/chitosan composite conduit) that must contain chitin.
2. Preparation of Chitosan is become the chitin fiber support: chitosan is dissolved in weak acid solution, weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), concentration is 2～15% (examples 2%, 8%, 15%), then solution is processed into chitin fiber by wet spinning technology; The alcoholic solution of the chitin fiber that obtains being put into acetic anhydride carries out acetylization reaction again, and reaction temperature is 0～60 ℃ (0 ℃, 30 ℃, 60 ℃ of examples), and the response time is 0.5～6 hour (example 0.5 hour, 2 hours, 4 hours, 6 hours).In the alcoholic solution of acetic anhydride, the concentration of acetic anhydride is 1～50% (example 1%, 20%, 40%, 50%), and alcoholic solution is methanol (or ethanol).Water cleans behind the acetylization reaction, puts into the sodium hydroxide of 1mol/L or potassium hydroxide solution then 24 hours, and water cleans up, and drying obtains the chitin fiber that degree of acetylation is 30-100%.
3. combination: will embed in the chitin/chitosan composite conduit that 1. step makes through the chitin fiber support that 2. step makes, obtain chitin/chitosan composite medical nerve graft.
Claims (6)
1, a kind of preparation method that contains the medical nerve graft of chitin is characterized in that: comprise the following steps:
1. Preparation of Chitosan is become to contain the conduit of chitin;
2. Preparation of Chitosan is become the chitin fiber support;
3. combination: the chitin fiber support embedding that makes is contained in the conduit of chitin;
The concrete grammar that step becomes to contain the conduit of chitin with Preparation of Chitosan in 1. is: earlier chitosan is made powder, then the chitosan powder is carried out acetylization reaction, obtaining degree of acetylation is 30～100% chitin powder; Other gets chitosan be dissolved into solution with weak acid after, add the above-mentioned chitin powder that obtains, mix homogeneously, then with mixture by the tubulose mould, and molding in alkaline solidification liquid after the molding is cleaned the conduit water, drying must contain the conduit of chitin;
Step becomes the concrete grammar of chitin fiber support to be Preparation of Chitosan in 2.: chitosan is dissolved in weak acid solution, then solution is processed into chitin fiber by wet spinning technology; Again the chitin fiber that obtains is carried out acetylization reaction, obtain degree of acetylation and be 30～100% chitin fiber.
2, the preparation method that contains the medical nerve graft of chitin according to claim 1; it is characterized in that: acetylization reaction is to carry out in the alcoholic solution of acetic anhydride; reaction temperature is 0～60 ℃; response time is 0.5～6 hour; also through washing, and put into sodium hydroxide or potassium hydroxide solution is handled behind the acetylization reaction.
3, the preparation method that contains the medical nerve graft of chitin according to claim 2 is characterized in that: in the alcoholic solution of acetic anhydride, the concentration of acetic anhydride is 0.1～50%, and alcoholic solution is methanol or ethanol.
4, the preparation method that contains the medical nerve graft of chitin according to claim 1 is characterized in that: when chitin powder adding chitosan solution was prepared conduit, the consumption of chitin was 10～90% of chitosan and a chitin amount sum.
5, the preparation method that contains the medical nerve graft of chitin according to claim 1 is characterized in that: weak acid is acetic acid, phosphoric acid, citric acid or lactic acid, and concentration is 2～15%.
6, the preparation method that contains the medical nerve graft of chitin according to claim 1 is characterized in that: alkaline solidification liquid is the solution that contains sodium hydroxide 8～20% or potassium hydroxide 8～20% or sodium carbonate 8～20%.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100391928A CN100484496C (en) | 2005-04-26 | 2005-04-26 | Method for preparing chitin-containing medical nerve graft |
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| CNB2005100391928A CN100484496C (en) | 2005-04-26 | 2005-04-26 | Method for preparing chitin-containing medical nerve graft |
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| CN100484496C true CN100484496C (en) | 2009-05-06 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106729980B (en) * | 2016-12-30 | 2019-11-12 | 清华大学 | A kind of bionical nerve graft and preparation method thereof for peripheral nerve reparation |
| CN107411996A (en) * | 2017-05-18 | 2017-12-01 | 鑫缘茧丝绸集团股份有限公司 | A kind of facial mask of the fiber containing silk fibroin |
| CN106983707A (en) * | 2017-05-18 | 2017-07-28 | 鑫缘茧丝绸集团股份有限公司 | A kind of preparation method of the facial mask of the fiber containing silk fibroin |
| CN107469144B (en) * | 2017-07-29 | 2020-10-02 | 青岛慧生惠众生物科技有限公司 | Chitosan-based composite nerve conduit and preparation method and application thereof |
| CN112323181B (en) * | 2020-11-23 | 2022-03-04 | 江南大学 | Preparation method of high-performance chitosan fiber |
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Inventor after: Yang Yumin Inventor after: Gu Xiaosong Inventor after: Lu Shibi Inventor after: Ding Fei Inventor after: Wang Xiaodong Inventor after: Zhao Qing Inventor after: Peng Jiang Inventor after: Hu Wen Inventor before: Yang Yumin Inventor before: Gu Xiaosong Inventor before: Wang Xiaodong Inventor before: Ding Fei Inventor before: Hu Wen |
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Free format text: CORRECT: INVENTOR; FROM: YANG YUMIN GU XIAOSONG WANG XIAODONG DING FEI HU WEN TO: YANG YUMIN GU XIAOSONG LU SHIBI DING FEI WANG XIAODONG ZHAO QING PENG JIANG HU WEN |