The kind that tangleweed is cultured up to now, the cultivating seaweed that enters agriculture category has only five genus, i.e. sea-tangle, opotism algae, laver, fragrant plant mentioned in ancient texts and Eucheuma etc.History from the development of world's algae culturing can be divided into four periods: the propagation period that (1) is original; (2) manually increase propagation period of matrix; (3) utilize artificial seed and sowing breed period; (4) seed selection, breeding and introduce a fine variety developing period of renewal.At present, still do not propagate the report of Irish moss artificially.
Carragheen has another name called carrageenan, antler glue, is the algal polysaccharides that a class is extracted from marine red alga, and its chemical constitution is D-galactose and 3, and the linear polysaccharide compound that 6-dehydration-D-galactose residue is formed has ester formula sulfate group on the residue.Since carragheen have moisture capacity, thickening and suspending, with premium properties such as combination of proteins, obtained extensive use in a lot of fields.
Different according to the arrangement position of sulfate group and quantity, carragheen can be divided into κ, ι, λ three major types type substantially, derives from different marine algas respectively, also has different purposes respectively.Carragheen in the market, κ-type carragheen especially, great majority are extractions from the Eucheuma (can culture in a large number in the torrid zone) of torrid zone growth.
Irish moss is because the problem of culturing, and is that the carragheen output of raw material is also little with it.Irish moss is numerous to be born on the tranquil rock of locating of middle stormy waves.The young is born in autumn, and is ripe summer.Wild Irish moss (chonelrces ocellatus) arranged in that NORTH CHINA is coastal.The Irish moss of spontaneous growth comprises the frond of different generations, and its ratio often changes, and the tetrasporophyte that has occupies the majority, and the gametophyte that has occupies the majority, when tetrasporophyte occupies the majority in the raw material, and the viscosity height of product, gel does not belong to lambda-carrageenan.Otherwise then solidifiability is good, and viscosity is low, belongs to the K-carragheen.With the carragheen product that these raw materials are done, owing to be the indefinite mixture of a kind of ratio, its character is difficult to control.Especially lambda-carrageenan is difficult to obtain from other marine alga.Owing to lack purer tetrasporophyte Irish moss, therefore be difficult to industrialization directly produces lambda-carrageenan always.
If have purer tetrasporophyte Irish moss, can be lambda-carrageenan production raw material preferably is provided.
Purpose of the present invention just provides a kind of method of propagating Irish moss artificially.Change unordered, the unsettled situation of colloid proterties of grows wild generation in the past by propagating Irish moss artificially, controlled Irish moss residing vegetative period artificially according to producing needs, thereby produce a large amount of tetrasporophyte Irish moss, obtain high-quality, lambda-carrageenan raw material cheaply.
Another object of the present invention provides the Irish moss raw material that tetrasporophyte accounts for main body.
Another object of the present invention provides the method that liquid carragheen is directly produced in industrialization.
In a first aspect of the present invention, provide a kind of Irish moss to propagate production method artificially, it may further comprise the steps:
(a) artificial Irish moss is grown seedlings, large-scale farming is in the marine site, and wherein said Irish moss is in same vegetative period basically;
(b) sampling check Irish moss residing vegetative period;
(c) be in tetrasporophyte during the phase when most of Irish moss, the results Irish moss.
In a preference, in step (a) before, also comprise step: wild Irish moss is carried out artificial screening, domestication; With the indoors artificial breeding.
In another preference, large-scale farming adopts following method to carry out: the soft raft method of semi-floating or prolong rope clamp seedling method.
In a preference, described sampling check is by slice probing Irish moss residing vegetative period.
In a second aspect of the present invention, a kind of Irish moss raw material is provided, wherein at least 75%, preferably at least 80%, more preferably at least 85%, at least 90% Irish moss is the tetrasporophyte Irish moss best.This Irish moss raw material is specially adapted to make liquid carragheen.
In a third aspect of the present invention, the purposes of a kind of Irish moss of the present invention is provided, it is used to make lambda-carrageenan.
In a fourth aspect of the present invention, a kind of liquid carragheen production method is provided, it comprises step:
Clean;
Dry;
Boil glue;
Filter;
Bleaching;
With concentrated,
Wherein, this method is a raw material with the tetrasporophyte Irish moss, thereby makes the liquid carragheen that concentration is 2-5%.
Preferably, the described glue that boils carries out under the following conditions: temperature is 85-90 ℃, time 0.5-1 hour.And being chlorine dioxide with 30-80ppm, the blanching step of described liquid carragheen carries out.
As used herein, " tetrasporophyte Irish moss " refers to be in the Irish moss Irish moss in vegetative period.This term also comprises Irish moss and the Irish moss after certain processing (as shining dry treatment) after the results, as long as this Irish moss is in tetrasporophyte vegetative period when results.
" tetrasporophyte Irish moss raw material " refers to that tetrasporophyte Irish moss quantity is greater than 50% Irish moss raw material.Usually, in the tetrasporophyte Irish moss raw material of producing with the inventive method that goes out, have at least 75%, preferably at least 80%, more preferably at least 85%, at least 90% Irish moss is the tetrasporophyte Irish moss best.
Inventive point of the present invention is, by the biological engineering, make Irish moss (growing seedlings) be in same vegetative period basically, then with vegetative period essentially identical Irish moss propagate artificially, thereby produce a large amount of tetrasporophyte Irish moss, as the raw material of producing lambda-carrageenan.
In a preference, the inventor has taked primary marine alga (Irish moss) artificial screening, the domestication, again from indoor breeding to propagating this new model on a large scale artificially.Thereby make the more science of propagating artificially of Irish moss, operability is stronger.
A kind of preferred specific embodiments is as follows:
1. screening of primary marine alga and domestication
With the primary Irish moss marine alga of different waters, after the collection, screened, reject disease is arranged, thin and weak and qualitative variation.Place the seawater that is about to culture the marine site then, make it adapt to culture the marine site growing environment, for artificial breeding and breed are laid a solid foundation.
2. indoors artificial is grown seedlings
In autumn, under indoor room temperature, the Irish moss marine alga that domestication is good places the cement pit water body, makes it diffuse sporophyte, utilizes the net sheet to gather sporophyte (being similar to plant seed).
The sporophyte of collecting or be allowed to condition under the suitable temperature and grow into seedling under (the control temperature is greater than about 15 ℃) carries out large-scale farming (be similar to and use rice shoot) with the seedling that grows then.Perhaps, allow the sporophyte of collecting not germinate (the control temperature is lower than about 15 ℃), move to suitable period in ocean temperature then and culture the marine site, allow sporophyte germinate simultaneously and grow, thereby make Irish moss be in same vegetative period (be similar to and use seed) basically.
3. large-scale farming
For its seed can better be grown in natural water, spatfall net sheet moves in the spring and autumn next year moderate time of ocean temperature again and cultures the marine site, guarantees its survival rate and more helps growth of seedling.
In the present invention, a kind of cultural method is to adopt the soft raft of semi-floating that Irish moss is propagated artificially on a large scale.Every mu of average 3 table flotation rafts, 60 meters of every directors, wide 1.5 meters, comprise floating stalk, stick up cable, wood sticks up, floating bamboo, compositions such as short supporting leg.
Another kind of cultural method is to adopt the method for prolonging the rope clamp seedling that the Irish moss seedling is cultured.These cultural methods all are that the technical staff knows in the aquaculture field.
4. sampling is checked and results
Treat that the Irish moss frond is bigger, regularly gather sample, the slice probing interior generates in case find large quantities of tetrasporophytes, just can fish according to producing the required manpower of organizing.
The Irish moss raw material of producing like this is unified from generation to generation, and quality is high, the dependable performance of the lambda-carrageenan product of production.
Carragheen is in the market all sold with solid forms, and in actual use, need be heated melt into liquid to carragheen, time-consuming taking a lot of work.The liquid carragheen is higher to the purity requirement of raw material marine alga, should be lambda-carrageenan entirely basically, otherwise gel can take place, and can not become liquid.When using wild Irish moss raw material, can't (or being difficult to) directly make the liquid carragheen.
When selecting for use tetrasporophyte Irish moss of the present invention to be raw material, the routine techniques that utilizes the carragheen preparation to utilize, can make concentration is the liquid carragheen of 2-5%, and production cost is low, it is comparatively convenient to use.Its concrete production technology comprises that mainly Irish moss cleans, dries, boils glue, filtration, bleaching, step such as concentrates.These steps all are that the technical staff of carragheen production field is known.
In one embodiment, boil glue and carry out under the following conditions: temperature is 85-90 ℃, time 0.5-1 hour.
Because Irish moss itself has darker color, must bleach.Carrageenan molecule is a kind of macromolecular polysaccharide, easily degraded under common bleaching agent effect, and the present invention selects for use chlorine dioxide as bleaching agent, and carrageenan molecule is not had destruction substantially, and under low concentration satisfied bleaching effect can be arranged.In one embodiment of the invention, in boiling the glue process, use chlorine dioxide to bleach, and selection chlorine dioxide concentration scope is 30-80ppm.
The concentration of carragheen can change according to user's needs in the product, generally is controlled at 2-5%, and higher then being difficult to of concentration concentrates, and concentration is too low then can to increase carrying cost.Because product is a liquid, easily grows microorganism, must adds preservative, but must select the kind that permission is used in food or the cosmetics according to its purposes.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment 1
Culture Irish moss
In September, 1998, in black stone reef marine site, Dalian, adopt the soft raft of semi-floating that Irish moss is cultured, its specific implementation method is:
1. establish 3 table flotation rafts (60 meters of every directors, wide 1.5 meters) for every mu, comprise floating stalk, stick up cable, wood sticks up, floating bamboo, compositions such as short supporting leg.
2. indoors artificial being cultivated with the net sheet is that the sporophyte of carrier is transplanted on the buoyant raft, cultures the waters water temperature about 15 ℃.
3. generate tetrasporophyte through slice probing November next year, and from generation to generation consistent (tetrasporophyte accounts for more than 90%), every mu can be obtained 300 kilograms of dried food and nuts.
Embodiment 2
Culture Irish moss
In October, 1998, in Rong Cheng marine site, Shandong Province, adopt the method for prolonging the rope clamp seedling that Irish moss is cultured, its specific implementation method is:
1. will screen good former algae Irish moss, divide branch into about 10 grammes per square metres;
2. cultivation rope is set in seawater, the Irish moss branch is hung on the cultivation rope by the direct folder of about 15 centimetres spacing;
3. water temperature is about about 15 ℃, and generate tetrasporophyte through slice probing May next year, and from generation to generation consistent (tetrasporophyte accounts for more than 85%) gathers in the crops 150 kilograms of dried food and nuts for every mu.
Embodiment 3
Make the liquid carragheen
1. the Irish moss raw material that obtains among the embodiment 1 is cleaned, dried, take by weighing 10 kilograms of dried Irish moss.
2. Irish moss is added in the glue pot, add 300 kg water, and add chlorine dioxide, making the concentration of chlorine dioxide in water is 40ppm, boils the glue temperature and is controlled at 85-90 ℃, time 0.5-1 hour.
3. filter with plate and frame filter press while hot, obtain transparent supernatant liquid, concentration about 1%.
4. the liquid after filtering is put into vacuum concentrating apparatus, being concentrated into carrageenan concentrations is 2%.
5. add preservative Germall15, concentration is 3/1000ths.
This product is applicable among the cosmetics.At cream frost, cleaning solution etc., when containing carragheen and being one thousandth, can play effect moist, that preserve moisture.
Embodiment 4
Make the liquid carragheen
1. the Irish moss raw material that obtains among the embodiment 2 is cleaned, dried, take by weighing 10 kilograms of dried Irish moss.
2. Irish moss is added in the glue pot, add 300 kg water, and add chlorine dioxide, making the concentration of chlorine dioxide in water is 70ppm, boils the glue temperature and is controlled at 85-90 ℃, time 0.5-1 hour.
3. filter with plate and frame filter press while hot, obtain transparent supernatant liquid, concentration about 1%.
4. the liquid after filtering is put into vacuum concentrating apparatus, being concentrated into carrageenan concentrations is 4%.
5. add the preservative methyl hydroxybenzoate, concentration is 4/1000ths.
This product is applicable among the food.For example in milk, carrageenan concentrations is 3/10000ths o'clock, can play stabilize casein.
Should be understood that those skilled in the art can make various changes or modifications the present invention after having read above-mentioned instruction content of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.