CN1771789A - Marine cultivation method of Okinawa Cladosiphon okamuranus - Google Patents
Marine cultivation method of Okinawa Cladosiphon okamuranus Download PDFInfo
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Abstract
The marine cultivation process of Okinawa Cladosiphon okamuranus includes the following steps: 1. seeding Okinawa Cladosiphon okamuranus discal body; 2. collecting seedlings after the Okinawa Cladosiphon okamuranus discal body covers 70-80 % of the seedling net; and 3. marine cultivation of Okinawa Cladosiphon okamuranus in selected sea area with sea water temperature 18-26 deg.c via fixing proper planting facility in sea area with piles, setting the seedling net on the planting facility, setting fencing net to avoid fish invasion and conventional cultivation management. After cultivation for 60-150 days and growing Okinawa Cladosiphon okamuranus to length 20-30 cm, the Okinawa Cladosiphon okamuranus may be collected via underwater vacuum sucking while leaving the other part in water for further growth.
Description
Technical field
The present invention relates to the improvement of algae culture technique, specifically is the marine cultivation production method of a kind of Okinawa tap algae; It belongs to the algal cultivation technical field.
Background technology
China is algal cultivation big country.The cultivation of marine alga, process technology are advanced, and for example, tangleweeds such as sea-tangle, laver and asparagus are that the world comes out at the top on output.Because Chinese sea area exists algae cultivation and the uneven in development problem of animal-breeding.Have many marine sites only to carry out the breed of marine animal, only carry out the breed of single species even, yet this pattern causes that the cultivation water area ecological environment worsens, and causes the cultivated animals illness outbreak, is seriously restricting the sustainable development of marine animal aquaculture.In order to further develop the algae cultivation industry, the scientific worker has been developed undaria pinnitafida, the breed of fragrant plant mentioned in ancient texts and Sargassum fusiforme (Harv.) Setch, and in extensive cultivation, done very big effort, be successful technically.There is a kind of Okinawa tap algae (Cladosiphon okamuranus Tokida) to belong to Phaeophyta (Phaeophyta), rope algae section (Chordariaceae), tap Trentepohlia (Cladosiphon) originates in Japan.The frond of this Okinawa tap algae is thread, softness, brown or dark brown, long 20-50cm, diameter 3.0-4.0mm.It is rich in brown alga sulfated polysaccharide bioactivators such as (fucoidin).Studies show that Okinawa tap algae can be improved body immunity, and multiple medical functions such as antitumor, lipopenicillinase, pressure regulation are arranged, O-157 has obvious inhibitory action to the pathogenicity Escherichia coli.Because the existence temperature of Okinawa tap algae is 18～30 ℃, under field conditions (factors), different environmental conditions is very big to the influence of growing of Okinawa tap algae.Because 18～30 ℃ of water temperature range that Okinawa tap algae grows and requires make this tap algae under simple natural conditions, can not aestivate in the southern china marine site, can not survive the winter in the northern marine site of China.Therefore, a stage in the history of life of Okinawa tap algae need just can be spent under manually operated condition.How China cultivates Okinawa tap algae in the sea area on a large scale is scientific worker's research topic.
Summary of the invention
Goal of the invention of the present invention is: in order to alleviate algae cultivation and the uneven in development contradiction of animal-breeding that China sea area exists, for improving China people's quality of life, the problem of employment and the foreign exchange earning that solve China labour provide a valid approach.This just need provide the algae and the cultivation and production technology thereof of Okinawa tap algae new varieties.Because the seawater component difference (containing a large amount of assorted algaes in the seawater in the sea area that has) in sea area and the weather in sea area is different, so standardized management must be passed through in the sea area that the cultivation of Okinawa tap algae is cultured.Therefore, the marine cultivation production method of Okinawa tap algae arises at the historic moment.
The objective of the invention is to realize by following technical scheme, developed the marine cultivation production method of a kind of Okinawa tap algae, described Okinawa tap algae (Cladosiphon okamuranus Tokida) is a kind of thread brown alga, belongs to Phaeophyta (Phaeophyta), rope algae section (Chordariaceae), tap Trentepohlia (Cladosiphon) marine alga, its frond softness is thread, brown or dark brown, long 20～50cm, diameter 3.0～4.0mm; Described marine cultivation production method is as follows:
(1) guarantor's kind of Okinawa tap algae:
1. the adult of Okinawa tap algae is protected and is planted:
To be cut into 2～3 sections of the fronds of 3～5cm with the frond that the sterilization seawater is cleaned, and be embedded in the solid culture medium, sealing is 16 ± 2 ℃ in temperature, and intensity of illumination is 1000～2000lux, and the photoperiod is to protect under the 12L/12D condition kind;
2. Okinawa tap algae discoid body is protected and is planted:
A) there is the dliploid sporangium that expands the assimilation silk end with Okinawa tap algae frond, and sporangium is chosen with capillary at microscopically, places the test tube that fills sterilization seawater nutrient solution;
B) above-mentioned test tube being placed on temperature is 23 ± 2 ℃, and intensity of illumination is 7000lux, and the photoperiod is to cultivate under the 12L/12D condition;
C) when discoid body occurring on the test tube wall, with the discoid body sucking-off, transfer in several triangular flasks that fill sterilization seawater nutrient solution, be 23 ± 2 ℃ in temperature, intensity of illumination is 7000lux, the photoperiod is to cultivate 30～40 days under the condition of 12L/12D;
D) above-mentioned triangular flask being placed on temperature is 16 ± 2 ℃, and intensity of illumination is/2000～3000lux, and the photoperiod is to protect under the 12L/12D condition to plant, and is standby;
(2) tap algae in Okinawa is collected seedling:
1. the container washing and sterilizing of will collecting seedling;
2. the seedling net is fully soaked in fresh water, cleans, drop in the container of collecting seedling;
3. the seawater nutrient solution of will sterilizing is poured in the container of collecting seedling;
4. Okinawa tap algae discoid body algae liquid that will protect kind is poured in the container of collecting seedling, and the cultivation temperature of collecting seedling is controlled between 20～26 ℃, and intensity of illumination 10000～50000lux collects seedling and cultivates, and changes the seawater nutrient solution of once sterilizing in 20～30 days;
5. the incubation time of collecting seedling is 30～50 days, when the surface of the lace curtaining 70～80% of seedling net is covered by tap algae discoid body, is the suitable density of collecting seedling;
(3) cultivate on Okinawa tap segasso sea:
1. select the sea area, the natural waters of cultivation Okinawa tap algae should possess following condition: clean water quality, and trend is unobstructed, and stormy waves is less, and seabed matter is the smooth husky end;
2. cultivating ocean temperature is 18～26 ℃, and the cultivation seawater salinity is 20～50, and the cultivation seawater transparency is 3～7m, and the cultivation sea area depth of water time of low water is 1～10m;
3. the setting of cultivating facility: select suitable cultivating facility to establish stake according to the sea area substrate and be fixed in the seabed; The seedling net of adopting placed on the cultivating facility cultivate;
4. for preventing the infringement of the natural enemy of fish, adopt the sea area purse seine structure that constitutes by long bamboo pole and the net sheet that is trapped among long bamboo pole outside at 2～3m place, cultivating facility periphery;
5. the management during the cultivation of Okinawa tap algae:
A) covered by discoid body when the surface of seedling net 70-80%, the sea area water temperature ties together a plurality of seedling nets between 18～26 ℃ the time, hangs on the buoyant raft and supports 20～30 days temporarily;
B) subnetting cultivation, seedling net spacing 80～90cm, the depth of water 1～5m supports the seedling net with bamboo pole, launches to hang on the cultivating facility;
C) scrub, in time scrub the seedling net after the subnetting cultivation, remove attached mud and assorted algae; When frond grows to 0.5cm when above, no longer scrub the seedling net;
D) increase buoyancy, suitably increase the quantity of float, keep the depth of water 1～5m of seedling net according to the upgrowth situation of frond;
E) reinforcement of raft frame is carried out in the reinforcing of raft frame, prevents that the seedling net from twining mutually or come off;
F) strengthen current check, in time prevent harmful organisms such as fish;
G) through 60～150 days cultivation, when frond length can reach 20～30cm, just can progressively stay seedling to gather, stay the continued growth of part frond;
H) method that adopts submersible vacuum to draw is drawn onto seawater and tap algae on the collection ship, discharges seawater; Clean with fresh water, pick up except that foreign material, take by weighing the pack of 5～10kg frond, quick-frozen stores.
Described solid culture medium, its manufacturing process is as follows: the ratio of Provasoli medium in 5～10mL/L is added in the seawater, and the ratio in 10～14g/L adds agar then, boils, and treats that temperature drops in the container of pouring sterilization after 50 ℃ rapidly into; After waiting to condense, plastic film glue envelope, standby.
Described sterilization seawater nutrient solution, process for preparation is as follows: the seawater that the Provasoli medium is added to sterilization in the ratio of 5～10mL/L, ratio in 50～100 μ g/l adds vitamin B1 then, ratio in 0.1～0.2 μ g/L adds cobalamin, ratio in 0.5～1 μ g/L adds vitamin h, mix, sealing, standby.
The cultivating facility of the marine cultivation and production of a kind of Okinawa as described tap algae.Described marine cultivating facility is the raft frame, and it comprises: floating well rope, stake cable, timber or anchor, float, seedling net and dropstone; Described floating well rope, it is fastened float and floats on the sea by tying up; Described float, it is tied up and fastens on floating well rope, ties up every 3～4m and fastens one; Described seedling net, its seedling net both sides are supported with bamboo pole, and this bamboo pole is tied up and is tethered on the floating well rope, and four jiaos of this seedling net are fixed on the floating well rope by hawser, and this seedling net also is sunken to the degree of depth certain below the sea by dropstone; By the seedling net that bamboo pole supports, dropstone, float and floating well rope have been formed the raft body; Described stake cable, it is used for fixing the raft body, and the one end links to each other with floating well rope, and the other end ties up on the timber or anchor that is fixed in the seabed.
Described floating well rope, it is the hawser of diameter 12～16mm, length is 55～65m.
Described seedling net, it is by limit guiding principle and nettle, and the button that wears long is knitted to connect and is formed, and its mesh is the net sheet of 30 * 30cm; Described seedling net size: length * wide=18 * 1.6m; Be arranged in parallel and tie up the seedling net that is tethered on the floating well rope, its spacing is provided with 15～20 seedling nets at 80～90cm on the floating well rope of every row;
Described stake cable, it is the hawser of diameter 18～20cm, this cable length is not less than 5 times of the high water time depth of water.
The invention has the advantages that: because in the marine cultivation production method step of Okinawa tap algae, at first started the protecting method of Okinawa tap algae: 1. the adult of Okinawa tap algae protect kind and 2. Okinawa tap algae discoid body protect kind; Especially the dliploid sporangium that the assimilation silk end of Okinawa tap algae frond is expanded is chosen sporangium with capillary at microscopically, places the test tube that fills sterilization seawater nutrient solution; And be 23 ± 2 ℃ in temperature, intensity of illumination is 7000lux, the photoperiod is to cultivate the dliploid spore under the 12L/12D condition, makes the discoid body of this spore ramp in stablizing fine environment in the test tube; When more discoid body occurring on the test tube wall, with the discoid body sucking-off, transfer in several triangular flasks that fill sterilization seawater nutrient solution, under aforementioned stable fine environment condition, cultivated 30～40 days; When occurring the discoid body of a large amount of growth growths on the triangular flask wall, promptly in the middle of discoid body, grow the assimilation silk, after the discoid body of this moment reaches maximum gauge, the assimilation silk becomes the period of organic centre, it is 16 ± 2 ℃ that above-mentioned triangular flask is placed on relatively low temperature, intensity of illumination is/2000～3000lux, and the photoperiod is that following guarantor of the rejection condition of 12L/12D planted, and can keep the activity of Okinawa tap algae the long period like this.Cultivation that this discoid body protecting method helps collecting seedling in the next procedure and tap algae discoid body are in the covering growth on the lace curtaining surface of seedling net.The present invention has been owing to designed the cultivation temperature of collecting seedling and be controlled between 20～26 ℃, and intensity of illumination 10000～50000lux collects seedling and cultivates, and changes the seawater nutrient solution of once sterilizing in 20～30 days; The incubation time of collecting seedling is 30～50 days, when the surface of the lace curtaining 70～80% of seedling net is covered by tap algae discoid body, is the suitable density of collecting seedling; The discoid body vegetative stage of Okinawa tap algae is spent under manually operated condition, lay the first stone for cultivating on the tap segasso sea of Okinawa.Cultivation step on the tap segasso sea of Okinawa of the present invention; At first be the condition of the selection natural waters of cultivation Okinawa tap algae: clean water quality, trend is unobstructed, and stormy waves is less, and seabed matter is the smooth husky end; Next is that selection cultivation ocean temperature is 18～26 ℃, and the cultivation seawater salinity is 20～50, and the cultivation seawater transparency is 3～7m, and the cultivation sea area depth of water time of low water is 1～10m; Be the setting of cultivating facility once more: select suitable cultivating facility to establish stake according to the sea area substrate and be fixed in the seabed; The seedling net of adopting placed on the cultivating facility cultivate; For preventing the infringement of harmful animals such as fish or skeleton shrimp, adopt the sea area purse seine structure that constitutes by long bamboo pole and the net sheet that is trapped among long bamboo pole outside at 2～3m place, cultivating facility periphery; The particularly important is the management during the cultivation of Okinawa tap algae: as the subnetting cultivation, scrub the seedling net, increase the depth of water that buoyancy keeps the seedling net, the raft frame is reinforced and is prevented that the seedling net from twining mutually or come off, and strengthens current check and prevent harmful organisms such as fish; These cultivation steps make Okinawa tap algae can grow vigorously in its marine growing and cultivating phase, and its frond length can reach more than the 20cm.
Because the sterilization seawater nutrient solution of the present invention's design, process for preparation is as follows: the seawater that Provasoli medium (the Provasoli culture medium prescription sees Table 1) is added to sterilization in the ratio of 5～10mL/L, ratio in 50～100 μ g/L adds vitamin B1 then, ratio in 0.1～0.2 μ g/L adds cobalamin, ratio in 0.5～1 μ g/L adds vitamin h, mix sealing; Make the dliploid spore can be in stablizing fine environment the discoid body of ramp.
Because the marine cultivating facility of the present invention's design is the raft frame, it comprises: floating well rope, stake cable, timber or anchor, float, seedling net and dropstone; Described floating well rope, it is fastened float and floats on the sea by tying up; Described float, it is tied up and fastens on floating well rope, ties up every 3～4m and fastens one; Described seedling net, its seedling net both sides are supported with bamboo pole, and this bamboo pole is tied up and is tethered on the floating well rope, and four jiaos of this seedling net are fixed on the floating well rope by hawser, and this seedling net also is sunken to the degree of depth certain below the sea by dropstone; By the seedling net that bamboo pole supports, dropstone, float and floating well rope have been formed the raft body; Described stake cable, it is used for fixing the raft body, and the one end links to each other with floating well rope, and the other end ties up on the timber or anchor that is fixed in the seabed; Make the seedling net can keep the suitable depth of water, firm raft frame can prevent that the seedling net from twining mutually or come off.
Accompanying drawing and embodiment thereof
Embodiments of the invention further specify as follows in conjunction with the accompanying drawings:
Fig. 1 is the structural representation of cultivating facility raft frame on the tap segasso sea of Okinawa.
The marine cultivation production method of Okinawa tap algae of development, described Okinawa tap algae (Cladosiphonokamuranus Tokida) is a kind of thread brown alga, belong to Phaeophyta (Phaeophyta), rope algae section (Chordariaceae), tap Trentepohlia (Cladosiphon) marine alga, its frond softness, be thread, brown or dark brown, long 20～50cm, diameter 3.0～4.0mm; Described marine cultivation production method is as follows:
(1) guarantor's kind of Okinawa tap algae:
1. the adult of Okinawa tap algae is protected and is planted:
To be cut into 2～3 sections of the fronds of 3～5cm with the frond that the sterilization seawater is cleaned, and be embedded in the solid culture medium, sealing is 16 ± 2 ℃ in temperature, and intensity of illumination is 1000～2000lux, and the photoperiod is to protect under the 12L/12D condition kind;
2. Okinawa tap algae discoid body guarantor kind of process is as follows:
At first, Okinawa tap urceolata sporophyte is pulverized as far as possible, the dliploid sporangium of maturation is chosen with capillary at microscopically, place the test tube that fills 10 milliliters of sterilization seawater nutrient solutions, it is 23 ± 2 ℃ that test tube is placed on temperature, intensity of illumination is 7000lux, and the photoperiod is to cultivate under the 12L/12D condition.
Secondly, under this condition of culture, ripe diplontic sporangium diffuses out spore.Spore is attached on the test tube wall, carries out mitosis then, grows to be discoid body.When the discoid body diameter reaches 70 μ m, form silk at the middle part of discoid body.Discoid body continues to increase, and when the discoid body diameter reaches 150 μ m, grows a lot of assimilation silks from the discoid body middle part.Discoid body continues to increase, and the quantity of assimilation silk also increases, and the length of assimilation silk also increases.When diameter reached 650 μ m, discoid body just no longer increased, and the assimilation silk becomes organic centre.After the dliploid sporangium maturation, discharge diplontic spore.Carry out mitosis after spore adheres to again, so the biomass of Okinawa tap algae just enlarges and comes.
When the discoid body of a lot of circles occurring on the test tube wall, just with the discoid body sucking-off in the test tube, be transferred in the triangular flask that fills 200ml sterilization seawater nutrient solution, it is 23 ± 2 ℃ that triangular flask is placed on temperature, intensity of illumination is 7000lux, and the photoperiod is to cultivate under the 12L/12D condition.
When occurring a large amount of discoid bodies on the triangular flask wall, it is 16 ℃ that several triangular flasks are placed on temperature, and intensity of illumination is 3000lux, and the photoperiod is to protect under the condition of 12L/12D kind.At low temperature, low light is according under the intensity, and Okinawa tap algae only carries out faint breath and photosynthesis.So just, can keep the activity of Okinawa tap algae the long period.
(2) collecting seedling of Okinawa tap algae:
1. the container washing and sterilizing of will collecting seedling;
2. the seedling net is fully soaked in fresh water, clean, till no longer foam occurring.Seedling net input is collected seedling in the container, and the seedling net of Okinawa tap algae is with 90 silk blend ropes (45 of 45+polyethylene of vinylon), and the limit guiding principle is with 240 or 360 polyethylene ropes (examination sea area stormy waves situation and decide), the limit guiding principle is connected with wearing the method for button with nettle, mesh 30 * 30cm, the long 18m of seedling net, wide 1.6m;
3. the seawater nutrient solution of will sterilizing is poured in the container of collecting seedling;
4. Okinawa tap algae is protected the discoid body algae liquid of planting and pour in the container, the temperature of collecting seedling is controlled between 20～26 ℃; Changed the seawater nutrient solution of once sterilizing in per 30 days; When running into cloudy raining, the lid waterproof cloth is rainproof.
(3) cultivate on Okinawa tap segasso sea:
1. select the sea area of suitable tap algae cultivation
The cultivation sea area of Okinawa tap algae should possess following condition: clean water quality, meet the relevant requirements among the GB 3097, and trend is unobstructed, stormy waves is less, substrate with the smooth husky end for well, temperature is 18～26 ℃, salinity is 20～50, and transparency is not less than 3m, and the time of low water, the depth of water was between 1～10m;
2. the structure of cultivating facility:
1) the raft shelf structure is used in Okinawa tap algae cultivation: as shown in Figure 1, the raft frame is mainly by floating well rope 3, stake cable 2, timber 1, float 4, seedling net 5 and dropstone 6 compositions.Wherein, floating well rope 3 is selected the polyethylene rope of diameter 12～16mm for use, and its length is 55～65m, floats on the sea by float 4; Stake cable 2 and timber 1 are used for fixing the raft body, and a stake cable 2 is the polyethylene rope of diameter 18～20cm, and one links to each other with floating well rope 3, and the other end ties up on the seabed timber 1, when length is not less than full sea 5 of the depth of water times; Float 4 is tied up on floating well rope 3, and for floating well rope 3 provides buoyancy, per 3～4m ties up one, available mao bamboon tube, baton round and polyethylene; Seedling net 5 both sides are supported with bamboo pole, and bondage is on floating well rope 3 again for bamboo pole, and four angles of seedling net 5 are fixed on the floating well rope 3 by the polyethylene rope of long 1m, and seedling net 5 is arranged in parallel, spacing 80～90cm, and a row is provided with 20 seedling nets 5; Seedling net 5 is sunken to below the sea by dropstone 6.
2), then adopt the mode of sea area purse seine to cultivate if there are harmful organisms such as fish in the sea area.With timber the long bamboo pole more than the diameter 6cm is fixed in the seabed, bamboo pole upper end is at the seawater high water time 50cm that surfaces, and the spacing of bamboo pole is 3m; With the aperture is that the polythene net sheet of 1cm is trapped among the bamboo pole outside, and the net sheet is raised to the higher plane of principle and is suspended on the bamboo pole, and infraclass is close to and is fixed on the seabed, and the net sheet is about 3m apart from the distance of raft shelf structure.
3. cultivation management work on Okinawa tap segasso sea:
A) covered by discoid body when the surface of seedling net 70-80%, the sea area water temperature ties together a plurality of seedling nets between 18～26 ℃ the time, hangs on the buoyant raft and supports 20 days or 25 days or 30 days temporarily;
B) subnetting cultivation, seedling net spacing 80～90cm, the support of bamboo pole is crossed by seedling Netcom, launches to hang on the raft frame, makes the seedling net be in the depth of water 0～5m place;
C) scrub, in time scrub the seedling net after the subnetting cultivation, remove attached mud and assorted algae; When frond grows to 0.5cm when above, no longer scrub the seedling net;
D) increase buoyancy, suitably increase the quantity of float, keep the depth of water 1～5m of seedling net according to the upgrowth situation of frond;
E) reinforcement of raft frame is carried out in the reinforcing of raft frame, prevents that the seedling net from twining mutually or come off;
F) strengthen current check, in time prevent harmful organisms such as fish;
G) through 60～150 days cultivation, just can progressively stay seedling to gather, stay the continued growth of part frond;
4. gather
Okinawa tap algae was through vegetative period of 60～150 days, and frond length can reach more than the 20cm, just can progressively gather.The method of gathering and adopting submersible vacuum to draw is drawn onto seawater and algae on the collection ship, discharges seawater, cleans with fresh water, picks up except that foreign material, takes by weighing the pack of 5～10kg frond, and quick-frozen stores.Cultivate result of implementation on the tap segasso sea of Okinawa of the present invention:, successfully cultivate Okinawa tap algae, 300 kilograms of every per mu yields annual June to August in marine site, Chinese Qingdao; In marine site, NORTH CHINA gulf, successfully cultivate Okinawa tap algae annual January to May, and every per mu yield reaches 1.2 tons.
Table 1Provasoli culture medium prescription
| The deposit mother liquor composition | Concentration (g/L) | |
| N | NH 4NO 3 | 23.5 |
| P | NaH 2PO 4.2H 2O | 3.89 |
| II | Fe(NH4) 2(SO 4).6H 2O | 0.701 |
| Na 2EDTA | 0.66 | |
| III | Na 2EDTA | 1.0 |
| H 3BO 3 | 1.14 | |
| FeCl 3.6H 2O | 0.049 | |
| MnCl 2.4H 2O | 0.146 | |
| ZnSO 4.7H 2O | 0.022 | |
| CoCl 2.6H 2O | 0.004 |
Those of ordinary skill in the art can understand, and in protection scope of the present invention, makes amendment for the foregoing description, and it all is possible adding and replacing, and it does not all exceed protection scope of the present invention.
Claims (7)
1, the marine cultivation production method of a kind of Okinawa tap algae, described Okinawa tap algae (Cladosiphonokamuranus Tokida) is a kind of thread brown alga, belong to Phaeophyta (Phaeophyta), rope algae section (Chordariaceae), tap Trentepohlia (Cladosiphon) marine alga, its frond softness, be thread, brown or dark brown, long 20～50cm, diameter 3.0～4.0mm; It is characterized in that: described marine cultivation production method as follows:
(1) guarantor's kind of Okinawa tap algae:
1. the adult of Okinawa tap algae is protected and is planted:
To be cut into 2～3 sections of the fronds of 3～5cm with the frond that the sterilization seawater is cleaned, and be embedded in the solid culture medium, sealing is 16 ± 2 ℃ in temperature, and intensity of illumination is 1000～2000lux, and the photoperiod is to protect under the 12L/12D condition kind;
2. Okinawa tap algae discoid body is protected and is planted:
A) there is the dliploid sporangium that expands the assimilation silk end with Okinawa tap algae frond, and sporangium is chosen with capillary at microscopically, places the test tube that fills sterilization seawater nutrient solution;
B) above-mentioned test tube being placed on temperature is 23 ± 2 ℃, and intensity of illumination is 7000lux, and the photoperiod is to cultivate under the 12L/12D condition;
C) when discoid body occurring on the test tube wall, with the discoid body sucking-off, transfer in several triangular flasks that fill sterilization seawater nutrient solution, be 23 ± 2 ℃ in temperature, intensity of illumination is 7000lux, the photoperiod is to cultivate 30～40 days under the condition of 12L/12D;
D) above-mentioned triangular flask being placed on temperature is 16 ± 2 ℃, and intensity of illumination is/2000～3000lux, and the photoperiod is to protect under the 12L/12D condition to plant, and is standby;
(2) tap algae in Okinawa is collected seedling:
1. the container washing and sterilizing of will collecting seedling;
2. the seedling net is fully soaked in fresh water, cleans, drop in the container of collecting seedling;
3. the seawater nutrient solution of will sterilizing is poured in the container of collecting seedling;
4. Okinawa tap algae discoid body algae liquid that will protect kind is poured in the container of collecting seedling, and the cultivation temperature of collecting seedling is controlled between 20～26 ℃, and intensity of illumination 10000～50000lux collects seedling and cultivates, and changes the seawater nutrient solution of once sterilizing in 20～30 days;
5. the incubation time of collecting seedling is 30～50 days, when the surface of the lace curtaining 70～80% of seedling net is covered by tap algae discoid body, is the suitable density of collecting seedling;
(3) cultivate on Okinawa tap segasso sea:
1. select the sea area, the natural waters of cultivation Okinawa tap algae should possess following condition: clean water quality, and trend is unobstructed, and stormy waves is less, and seabed matter is the smooth husky end;
2. cultivating ocean temperature is 18～26 ℃, and the cultivation seawater salinity is 20～50, and the cultivation seawater transparency is 3～7m, and the cultivation sea area depth of water time of low water is 1～10m;
3. the setting of cultivating facility: select suitable cultivating facility to establish stake according to the sea area substrate and be fixed in the seabed; The seedling net of adopting placed on the cultivating facility cultivate;
4. for preventing the infringement of the natural enemy of fish, adopt the sea area purse seine structure that constitutes by long bamboo pole and the net sheet that is trapped among long bamboo pole outside at 2～3m place, cultivating facility periphery;
5. the management during the cultivation of Okinawa tap algae:
A) covered by discoid body when the surface of seedling net 70-80%, the sea area water temperature ties together a plurality of seedling nets between 18～26 ℃ the time, hangs on the buoyant raft and supports 20～30 days temporarily;
B) subnetting cultivation, seedling net spacing 80～90cm, the depth of water 1～5m supports the seedling net with bamboo pole, launches to hang on the cultivating facility;
C) scrub, in time scrub the seedling net after the subnetting cultivation, remove attached mud and assorted algae; When frond grows to 0.5cm when above, no longer scrub the seedling net;
D) increase buoyancy, suitably increase the quantity of float, keep the depth of water 1～5m of seedling net according to the upgrowth situation of frond;
E) reinforcement of raft frame is carried out in the reinforcing of raft frame, prevents that the seedling net from twining mutually or come off;
F) strengthen current check, in time prevent harmful organisms such as fish;
G) through 60～150 days cultivation, when frond length can reach 20～30cm, just can progressively stay seedling to gather, stay the continued growth of part frond;
H) method that adopts submersible vacuum to draw is drawn onto seawater and tap algae on the collection ship, discharges seawater; Clean with fresh water, pick up except that foreign material, take by weighing the pack of 5～10kg frond, quick-frozen stores.
2, according to the marine cultivation production method of the described Okinawa of claim 1 tap algae, it is characterized in that: described solid culture medium, its manufacturing process is as follows: the ratio of Provasoli medium in 5～10mL/L is added in the seawater, ratio in 10～14g/L adds agar then, boil, treat that temperature drops in the container of pouring sterilization after 50 ℃ rapidly into; After waiting to condense, plastic film glue envelope, standby.
3, according to the marine cultivation production method of the described Okinawa of claim 1 tap algae, it is characterized in that: described sterilization seawater nutrient solution, process for preparation is as follows: the seawater that the Provasoli medium is added to sterilization in the ratio of 5～10mL/L, ratio in 50～100 μ g/L adds vitamin B1 then, ratio in 0.1～0.2 μ g/L adds cobalamin, and the ratio adding vitamin h in 0.5～1 μ g/L mixes, sealing, standby.
4, the cultivating facility of the marine cultivation and production of a kind of Okinawa according to claim 1 tap algae, it is characterized in that: described marine cultivating facility is the raft frame, and it comprises: floating well rope, stake cable, timber or anchor, float, seedling net and dropstone; Described floating well rope, it is fastened float and floats on the sea by tying up; Described float, it is tied up and fastens on floating well rope, ties up every 3～4m and fastens one; Described seedling net, its seedling net both sides are supported with bamboo pole, and this bamboo pole is tied up and is tethered on the floating well rope, and four jiaos of this seedling net are fixed on the floating well rope by hawser, and this seedling net also is sunken to the degree of depth certain below the sea by dropstone; By the seedling net that bamboo pole supports, dropstone, float and floating well rope have been formed the raft body; Described stake cable, it is used for fixing the raft body, and the one end links to each other with floating well rope, and the other end ties up on the timber or anchor that is fixed in the seabed.
5, according to the cultivating facility of the marine cultivation and production of the described Okinawa of claim 4 tap algae, it is characterized in that: described floating well rope, it is the hawser of diameter 12～16mm, length is 55～65m.
6, according to the cultivating facility of the marine cultivation and production of the described Okinawa of claim 4 tap algae, it is characterized in that: described seedling net, it is by limit guiding principle and nettle, and the button that wears long is knitted to connect and is formed, and its mesh is the net sheet of 30 * 30cm; Described seedling net size: length * wide=18 * 1.6m; Be arranged in parallel and tie up the seedling net that is tethered on the floating well rope, its spacing is provided with 15～20 seedling nets at 80～90cm on the floating well rope of every row.
7, according to the cultivating facility of the marine cultivation and production of the described Okinawa of claim 4 tap algae, it is characterized in that: described stake cable, it is the hawser of diameter 18～20cm, this cable length is not less than 5 times of the high water time depth of water.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510104557 CN1771789A (en) | 2005-11-12 | 2005-11-12 | Marine cultivation method of Okinawa Cladosiphon okamuranus |
| CNA2006100769461A CN1843085A (en) | 2005-11-12 | 2006-04-13 | Cladosiphon okamuranusa marine cultivation method |
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| CN 200510104557 CN1771789A (en) | 2005-11-12 | 2005-11-12 | Marine cultivation method of Okinawa Cladosiphon okamuranus |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104170715A (en) * | 2014-08-21 | 2014-12-03 | 中国科学院海洋研究所 | Special fixture for algal reef construction and indoor and maritime hard algae collection matrixes |
| CN106386459A (en) * | 2016-12-12 | 2017-02-15 | 江苏省海洋水产研究所 | Nori cultivation simulation device |
| CN106804419A (en) * | 2017-01-21 | 2017-06-09 | 宁波大学 | A kind of fast Acquisition floats the device and its construction method of Sargassum horneri |
| CN115568415A (en) * | 2022-10-20 | 2023-01-06 | 温州科技职业学院 | Wisdom laver planting device |
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2005
- 2005-11-12 CN CN 200510104557 patent/CN1771789A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104170715A (en) * | 2014-08-21 | 2014-12-03 | 中国科学院海洋研究所 | Special fixture for algal reef construction and indoor and maritime hard algae collection matrixes |
| CN106386459A (en) * | 2016-12-12 | 2017-02-15 | 江苏省海洋水产研究所 | Nori cultivation simulation device |
| CN106386459B (en) * | 2016-12-12 | 2022-03-29 | 江苏省海洋水产研究所 | Simulation device for laver cultivation |
| CN106804419A (en) * | 2017-01-21 | 2017-06-09 | 宁波大学 | A kind of fast Acquisition floats the device and its construction method of Sargassum horneri |
| CN106804419B (en) * | 2017-01-21 | 2022-06-07 | 宁波大学 | Device for rapidly capturing floating sargassum horneri and construction method thereof |
| CN115568415A (en) * | 2022-10-20 | 2023-01-06 | 温州科技职业学院 | Wisdom laver planting device |
| CN115568415B (en) * | 2022-10-20 | 2024-06-11 | 温州科技职业学院 | Intelligent laver planting device |
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