CN1340613A - Process for immobilizing bacteria to biologically repair culture environment of aquatic products - Google Patents
Process for immobilizing bacteria to biologically repair culture environment of aquatic products Download PDFInfo
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- CN1340613A CN1340613A CN 00111306 CN00111306A CN1340613A CN 1340613 A CN1340613 A CN 1340613A CN 00111306 CN00111306 CN 00111306 CN 00111306 A CN00111306 A CN 00111306A CN 1340613 A CN1340613 A CN 1340613A
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Abstract
A process for immobilizing the bacteria to repair the environment of culturing aquatic products includes such steps as proportionally mixing the said bacteria able to degradate and transfer the organic pollutants with zeolite as carrier mixing them with zeolite proportionally, stirring to uniformity, shaking for a certain time, and adding it to culture pool. Its advantages include low cost and no secondary pollution.
Description
The invention belongs to culture environment of aquatic products improves and restorative procedure.In detail, be the process for fixation of a kind of culture environment of aquatic products biological restoration effect bacterium.
The deterioration of breeding environment has become the key factor that restricts China's culture fishery healthy and sustainable development.Therefore, it is a kind of effective to be badly in need of seeking, the method and the technology of the reparation breeding environment of strong operability.People are with the microbiological deterioration organic pollutant and the process of decontamination and environment purification is referred to as biological restoration (Bioremediation) or biological resuming (Biorestiration) (Madsen, 1991; Tomas, 1994).Because repairing, the microorganism of environment has less investment, the advantage of non-secondary pollution, thus more and more cause the attention of various countries, in the ascendant.
Though the history of the bioremediation technology of culture environment of aquatic products research is not long, has demonstrated huge vitality.The problem of project of national nature science fund project that Yuan Youxian etc. (2000) bear and the relevant shrimp of national 863 high-tech program projects pond organic contamination biodegradation technique aspect, succeed in developing serial breeding environment and repaired product, and successfully be applied to demonstrate tempting application prospect in the prawn culturing.The characteristics of aquaculture pond organic contamination are that movements such as residual bait and ight soil are deposited at the bottom of the pond, and therefore, only procreation could at utmost play a role functional bacteria in the bottom.There are several companies to produce purification of water quality microbial inoculums (powder) abroad as " Probiltics " (Enviro-Reps Internatonal L.C.), Epicin (Canadian Epicore network company) etc., Yu Weijun etc. (1991) once used photosynthetic bacterium and improved water quality, can eliminate organism in the water, but the effect of organic matter degradation at the bottom of the pond is restricted.Focusing on of industrial sewage all is that functional bacteria is fixed on the different chemical materials, uses calcium alginate gel embedding candida tropicalis Phenol-Containing Wastewater Treatment as (1990) such as Zhou Ding; Liu Zhipei (1992) decolours to dyeing waste water with polyvinyl alcohol immobilized mixt bacteria cell; People such as Portier (1988) utilize fixedly pseudomonas of porous carrier Celite R-630 absorption, handle the sterilant waste water that contains chloracetate.These chemical material prices are higher, and are not only uneconomical as using in aquaculture, and can cause the secondary pollution in pond.
Zeolite is a kind of natural clay mineral, and there is abundant mining deposits in China.Because of zeolite has the absorption characteristic of ammonia nitrogen and cheap, in culture fishery, be extensive use of to improve water quality (Yuan Youxian etc., 1987).But utilize zeolite as fixation support, the research of fixed action bacterium does not appear in the newspapers as yet.
The objective of the invention is to seek a kind of immobilization material and method, can efficiently degrade or transform the functional bacteria immobilization of organic pollutants such as the residual bait in aquaculture pond bottom, ight soil fast, make the product of being convenient to preservation, transportation, after making the product input culture the pond, can be sunken at the bottom of the pond, with organic contamination at the bottom of the quick elimination pond, for aquatic animal is built a good growth habitat.
Technical scheme of the present invention is achieved in that with the zeolite to be carrier, the functional bacteria immobilization that will have stronger degradation capability, can grow under the normal temperature in briny environment, multiply the residual bait and the ight soil of aquatic animal.Selecting particle diameter is 40～80 purpose zeolites, after the calcination activation, in 1: 0.003: 1 ratio (weight ratio) and thalline and sea water mixing, stirs, and jolting 20～60min leaves standstill 1～2hr, and functional bacteria fully is adsorbed in the surface and hole of zeolite.Empty solid carbon dioxide branch obtains product, vacuum-packed, normal temperature storage.In the different breeding phase, by per hectare 110～180kg consumption product is applied the breed pond, functional bacteria can be bred in the bottom, and the sedimentary organic pollutant of degrading is repaired breeding environment.
Be described in detail operative technique content of the present invention below in conjunction with embodiment.Embodiments of the invention are to utilize the immobilized functional bacteria of zeolite (as Bacillus circulans, bacillus firmus and subtilis etc.) to repair the prawn culturing sedimentary environment.
Concrete operation method is as follows:
(1) zeolite pre-treatment: at first sieve is got 40～80 purpose zeolites, and calcination activation 2hr cools off standby.
(2) functional bacteria preparation: thalline is made into the bacteria suspension that concentration is 3g/L with seawater, standby.
(3) immobilization of thalline: get a certain amount of bacteria suspension, the zeolite that weight such as adding are handled well stirs, and jolting 30min leaves standstill 2hr, and bacterium is adsorbed in zeolite surface and the hole.Pour container hollow solid carbon dioxide branch into, get the immobilization product, pack, vacuum packaging.
(4) repairing product uses: during culturing, by per hectare 150kg consumption, product evenly is sprinkled into shrimp culture pond, used once in per about 10 days, this product can sink to cultures the bottom, pond, and functional bacteria is bred fast in the bottom, sedimentary and the settled successively organic pollutant of degrading, making it in time be converted into the inorganic nutrient salt that can be utilized by plant plankton, thereby reached the reparation breeding environment, is the purpose that cultivated animals is built good growth habitat.
The characteristics that the present invention has are: 1. the present invention activates by calcination, will be adsorbed on the zeolite crystal sky The water of hole and duct surfaces externally and internally discharges, and its cavity and duct is increased, thereby increase its surface Long-pending, be beneficial to the absorption of bacterium; 2. because zeolite proportion is bigger, functional bacteria can be fast after the zeolite immobilization Speed is sunken to the culturing pool bottom, in time plays a role and difficult the loss, has improved functioning efficiency; 3. select Zeolite not only can make functional bacteria fall at the bottom of the pond rapidly, and the NH in the zeolite energy adsorbed water itself3、
H
2S and organic Humus, and can carry oxygen at the bottom of the pond, increase dissolved oxygen amount; 4. zeolite does not contain nuisance, Can not cause secondary pollution to water body; 5. select zeolite as the thalline fixation support, the raw material mineral reserve are rich Richness, easily exploitation, processing, cheap, immobilization technology is simple.
Claims (2)
1. the process for fixation of a bacteria to biologically repair culture environment of aquatic products is characterized in that the functional bacteria immobilization material of selecting for use is a zeolite.
2. the process for fixation of a kind of bacteria to biologically repair culture environment of aquatic products according to claim 1, it is characterized in that described immobilization material zeolite activates through calcination, the functional bacteria thalline is made certain density bacteria suspension, add the certain proportion zeolite, vibration, leave standstill, functional bacteria is adsorbed in zeolite surface and the hole, make solid phase prod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00111306 CN1340613A (en) | 2000-08-25 | 2000-08-25 | Process for immobilizing bacteria to biologically repair culture environment of aquatic products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00111306 CN1340613A (en) | 2000-08-25 | 2000-08-25 | Process for immobilizing bacteria to biologically repair culture environment of aquatic products |
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| Publication Number | Publication Date |
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| CN1340613A true CN1340613A (en) | 2002-03-20 |
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| CN 00111306 Pending CN1340613A (en) | 2000-08-25 | 2000-08-25 | Process for immobilizing bacteria to biologically repair culture environment of aquatic products |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100343185C (en) * | 2004-06-08 | 2007-10-17 | 张广志 | Ecological water purifying material for aquiculture |
| CN101177680B (en) * | 2006-11-10 | 2010-09-29 | 中国科学院沈阳应用生态研究所 | Method for preparing mixed bacteria immobilized particle |
| CN102432110A (en) * | 2011-10-28 | 2012-05-02 | 浙江工商大学 | River sediment biology base degradation composite and application thereof |
| CN103045579A (en) * | 2012-12-17 | 2013-04-17 | 南开大学 | Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same |
| CN106745821A (en) * | 2017-02-22 | 2017-05-31 | 大连理工大学 | Sea-farming special microorganism water purification agent and preparation method thereof and application method |
-
2000
- 2000-08-25 CN CN 00111306 patent/CN1340613A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100343185C (en) * | 2004-06-08 | 2007-10-17 | 张广志 | Ecological water purifying material for aquiculture |
| CN101177680B (en) * | 2006-11-10 | 2010-09-29 | 中国科学院沈阳应用生态研究所 | Method for preparing mixed bacteria immobilized particle |
| CN102432110A (en) * | 2011-10-28 | 2012-05-02 | 浙江工商大学 | River sediment biology base degradation composite and application thereof |
| CN102432110B (en) * | 2011-10-28 | 2013-06-12 | 浙江工商大学 | River sediment biology base degradation composite and application thereof |
| CN103045579A (en) * | 2012-12-17 | 2013-04-17 | 南开大学 | Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same |
| CN103045579B (en) * | 2012-12-17 | 2014-10-15 | 南开大学 | Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same |
| CN106745821A (en) * | 2017-02-22 | 2017-05-31 | 大连理工大学 | Sea-farming special microorganism water purification agent and preparation method thereof and application method |
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