CN1318098A - 用于反相离子对高效液相层析分离核酸的单片基质 - Google Patents
用于反相离子对高效液相层析分离核酸的单片基质 Download PDFInfo
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- CN1318098A CN1318098A CN99811045.0A CN99811045A CN1318098A CN 1318098 A CN1318098 A CN 1318098A CN 99811045 A CN99811045 A CN 99811045A CN 1318098 A CN1318098 A CN 1318098A
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
- B01J20/285—Porous sorbents based on polymers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/366—Ion-pair, e.g. ion-pair reversed phase
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
- B01J20/267—Cross-linked polymers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28042—Shaped bodies; Monolithic structures
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/305—Addition of material, later completely removed, e.g. as result of heat treatment, leaching or washing, e.g. for forming pores
- B01J20/3064—Addition of pore forming agents, e.g. pore inducing or porogenic agents
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/52—Physical parameters
- G01N2030/524—Physical parameters structural properties
- G01N2030/528—Monolithic sorbent material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
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- Crystallography & Structural Chemistry (AREA)
- Thermal Sciences (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Saccharide Compounds (AREA)
- Polymerisation Methods In General (AREA)
Abstract
Description
名称 | 固定相 | 相互作用 |
大小排阻层析 | 多孔惰性颗粒 | 样品按其在溶液中的大小分开;不同大小的分子通过柱的总渡越时间不同。 |
离子交换层析 | 树脂上的离子基 | 样品离子与填料上已有的离子化基团上的离子交换;保留时间取决于处于该位的不同离子的亲和力和溶液的其它参数(pH、离子强度、抗衡离子类型等) |
反相层析 | 树脂上的非极性基团 | 按与固定相之间的疏水相互作用分离样品 |
填装无孔颗粒的柱 | C12单片聚合物的柱 | |
峰1的保留时间(分钟) | 6.80 | 9.36 |
峰1的面积(μV×秒) | 80678.00 | 85692.00 |
峰1的高度(μV) | 11370.00 | 7438.00 |
峰2的保留时间(分钟) | 7.52 | 10.10 |
峰2的面积(μV×秒) | 103115.00 | 103644.00 |
峰2的高度(μV) | 13348.00 | 9779.00 |
分离度 | 5.83 | 4.04 |
柱压(psi) | 1340.00 | 100.00 |
表观颗粒尺寸(μm) | 1.20 | 6.20 |
期望分离度 | 5.83 | 2.57 |
超额分离度% | 0.00 | 58.00 |
分离度/1,000psi | 4.35 | 40.42 |
流动相: | A:20mM溴化四丙铵+2mMEDTA二钠(用水配制),pH7.0B:用乙腈配制的20mM溴化四丙铵 |
梯度: | 50-65%B在A中(为时7分钟) |
流速: | 0.75ml/分钟 |
分离温度: | 43℃ |
探测: | UV,254nm |
Claims (21)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/154,529 | 1998-09-16 | ||
US09/154,529 US6238565B1 (en) | 1998-09-16 | 1998-09-16 | Monolithic matrix for separating bio-organic molecules |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1318098A true CN1318098A (zh) | 2001-10-17 |
CN1318585C CN1318585C (zh) | 2007-05-30 |
Family
ID=22551684
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB998110450A Expired - Fee Related CN1318585C (zh) | 1998-09-16 | 1999-09-08 | 用于反相离子对高效液相层析分离核酸的单片基质 |
Country Status (7)
Country | Link |
---|---|
US (1) | US6238565B1 (zh) |
EP (1) | EP1114150B1 (zh) |
JP (1) | JP4593785B2 (zh) |
CN (1) | CN1318585C (zh) |
CA (1) | CA2344051C (zh) |
DE (2) | DE69930757T2 (zh) |
WO (1) | WO2000015778A1 (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102680601A (zh) * | 2011-12-20 | 2012-09-19 | 山东凯盛新材料股份有限公司 | 乳粉中核苷酸高效液相色谱测定方法 |
CN101939094B (zh) * | 2008-02-05 | 2013-11-20 | 通用电气健康护理生物科学股份公司 | 分离介质的制备方法 |
CN103443622A (zh) * | 2011-03-22 | 2013-12-11 | 积水医疗株式会社 | 液相色谱法用柱和血红蛋白类的分析方法 |
CN104892710A (zh) * | 2015-05-19 | 2015-09-09 | 邦泰生物工程(深圳)有限公司 | 一种纯化还原型β-烟酰胺腺嘌呤二核苷酸的方法 |
Families Citing this family (50)
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US6576133B2 (en) | 1996-11-13 | 2003-06-10 | Transgenomic, Inc | Method and system for RNA analysis by matched ion polynucleotide chromatography |
US7138518B1 (en) | 1996-11-13 | 2006-11-21 | Transgenomic, Inc. | Liquid chromatographic separation of polynucleotides |
US6475388B1 (en) | 1996-11-13 | 2002-11-05 | Transgenomic, Inc. | Method and system for RNA analysis by matched ion polynucleotide chromatography |
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US6503397B2 (en) * | 1997-12-05 | 2003-01-07 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
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US20070161010A1 (en) * | 1998-08-04 | 2007-07-12 | Gjerde Douglas T | Methods and compositions for mutation analysis |
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US20030230524A1 (en) * | 2002-06-18 | 2003-12-18 | Naohiro Soga | Chromatographic chip and method of fabrication thereof |
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1998
- 1998-09-16 US US09/154,529 patent/US6238565B1/en not_active Expired - Fee Related
-
1999
- 1999-09-08 CN CNB998110450A patent/CN1318585C/zh not_active Expired - Fee Related
- 1999-09-08 CA CA2344051A patent/CA2344051C/en not_active Expired - Fee Related
- 1999-09-08 WO PCT/US1999/020596 patent/WO2000015778A1/en active IP Right Grant
- 1999-09-08 DE DE69930757T patent/DE69930757T2/de not_active Expired - Lifetime
- 1999-09-08 EP EP99946805A patent/EP1114150B1/en not_active Expired - Lifetime
- 1999-09-08 DE DE1114150T patent/DE1114150T1/de active Pending
- 1999-09-08 JP JP2000570305A patent/JP4593785B2/ja not_active Expired - Fee Related
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101939094B (zh) * | 2008-02-05 | 2013-11-20 | 通用电气健康护理生物科学股份公司 | 分离介质的制备方法 |
CN103443622A (zh) * | 2011-03-22 | 2013-12-11 | 积水医疗株式会社 | 液相色谱法用柱和血红蛋白类的分析方法 |
CN103443622B (zh) * | 2011-03-22 | 2015-10-14 | 积水医疗株式会社 | 液相色谱法用柱和血红蛋白类的分析方法 |
CN102680601A (zh) * | 2011-12-20 | 2012-09-19 | 山东凯盛新材料股份有限公司 | 乳粉中核苷酸高效液相色谱测定方法 |
CN104892710A (zh) * | 2015-05-19 | 2015-09-09 | 邦泰生物工程(深圳)有限公司 | 一种纯化还原型β-烟酰胺腺嘌呤二核苷酸的方法 |
CN104892710B (zh) * | 2015-05-19 | 2017-09-29 | 邦泰生物工程(深圳)有限公司 | 一种纯化还原型β‑烟酰胺腺嘌呤二核苷酸的方法 |
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EP1114150B1 (en) | 2006-04-05 |
US6238565B1 (en) | 2001-05-29 |
DE1114150T1 (de) | 2001-12-20 |
CA2344051C (en) | 2010-03-16 |
JP4593785B2 (ja) | 2010-12-08 |
DE69930757T2 (de) | 2007-01-04 |
CA2344051A1 (en) | 2000-03-23 |
DE69930757D1 (de) | 2006-05-18 |
CN1318585C (zh) | 2007-05-30 |
EP1114150A1 (en) | 2001-07-11 |
JP2002525558A (ja) | 2002-08-13 |
WO2000015778A1 (en) | 2000-03-23 |
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