CN1316885C - Method for protecting living body in radialization of UV-B using catechuic acid - Google Patents
Method for protecting living body in radialization of UV-B using catechuic acid Download PDFInfo
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- CN1316885C CN1316885C CNB2004100892363A CN200410089236A CN1316885C CN 1316885 C CN1316885 C CN 1316885C CN B2004100892363 A CNB2004100892363 A CN B2004100892363A CN 200410089236 A CN200410089236 A CN 200410089236A CN 1316885 C CN1316885 C CN 1316885C
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- catechin
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- 229950001002 cianidanol Drugs 0.000 title claims abstract description 43
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 12
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims abstract description 42
- 235000005487 catechin Nutrition 0.000 claims abstract description 42
- 230000005855 radiation Effects 0.000 claims abstract description 25
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 claims abstract description 15
- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 claims abstract description 14
- LSHVYAFMTMFKBA-TZIWHRDSSA-N (-)-epicatechin-3-O-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=CC=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 LSHVYAFMTMFKBA-TZIWHRDSSA-N 0.000 claims abstract description 10
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 claims abstract description 8
- LSHVYAFMTMFKBA-UHFFFAOYSA-N ECG Natural products C=1C=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 LSHVYAFMTMFKBA-UHFFFAOYSA-N 0.000 claims abstract description 6
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229960001948 caffeine Drugs 0.000 claims abstract description 4
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000002904 solvent Substances 0.000 claims abstract description 3
- 229940030275 epigallocatechin gallate Drugs 0.000 claims description 12
- 239000007921 spray Substances 0.000 claims description 10
- 238000012423 maintenance Methods 0.000 claims description 5
- 230000008439 repair process Effects 0.000 abstract description 3
- 238000005507 spraying Methods 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 description 17
- 239000007864 aqueous solution Substances 0.000 description 6
- 244000269722 Thea sinensis Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 241000219194 Arabidopsis Species 0.000 description 4
- XMOCLSLCDHWDHP-IUODEOHRSA-N epi-Gallocatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-IUODEOHRSA-N 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
- 235000013824 polyphenols Nutrition 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 2
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XMOCLSLCDHWDHP-UHFFFAOYSA-N L-Epigallocatechin Natural products OC1CC2=C(O)C=C(O)C=C2OC1C1=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-UHFFFAOYSA-N 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- 240000003152 Rhus chinensis Species 0.000 description 2
- 235000014220 Rhus chinensis Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N anhydrous gallic acid Natural products OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 230000003266 anti-allergic effect Effects 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000035619 diuresis Effects 0.000 description 2
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 2
- 235000012734 epicatechin Nutrition 0.000 description 2
- DZYNKLUGCOSVKS-UHFFFAOYSA-N epigallocatechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3cc(O)c(O)c(O)c3 DZYNKLUGCOSVKS-UHFFFAOYSA-N 0.000 description 2
- 230000002218 hypoglycaemic effect Effects 0.000 description 2
- 238000001953 recrystallisation Methods 0.000 description 2
- 102000004118 Ammonia-Lyases Human genes 0.000 description 1
- 108090000673 Ammonia-Lyases Proteins 0.000 description 1
- 108700013749 Arabidopsis AT5G13930 Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- OEIJRRGCTVHYTH-UHFFFAOYSA-N Favan-3-ol Chemical compound OC1CC2=CC=CC=C2OC1C1=CC=CC=C1 OEIJRRGCTVHYTH-UHFFFAOYSA-N 0.000 description 1
- CITFYDYEWQIEPX-UHFFFAOYSA-N Flavanol Natural products O1C2=CC(OCC=C(C)C)=CC(O)=C2C(=O)C(O)C1C1=CC=C(O)C=C1 CITFYDYEWQIEPX-UHFFFAOYSA-N 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- -1 catechin gallic acid ester Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000011987 flavanols Nutrition 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002746 orthostatic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention uses catechin to protect organisms radiated by UV-B, namely that the present invention uses the catechin to prevent the organisms from being injured by UV-B radiation, or the present invention uses the catechin to repair the organisms injured by UV-B radiation. The content of the (-)-epigallocatechin-3-gallate (EGCG) in the catechin is no less than 40%, the content of the (-)-epicatechin gallate (ECG) in the catechin is no less than 15%, and the content of caffeine is no less than 2.5%. The use concentration of the catechin is each liter of solvent contains more than 100 milligrams of catechin. In the use method, the radiated organisms are protected with a method that the radiated organisms are sprayed or smeared with the catechin before being radiated by UV-B, and the radiated organisms are repaired with a method of multiple time spraying or smearing after being injured by UV-B radiation.
Description
Technical field
The present invention relates to the new purposes of effective content of tea, specifically the organism that is subjected to the uv b radiation damage with prevention of effective content of tea catechin and maintenance.
Background technology
Catechin and derivative thereof belong to phenols.Catechin is a flavanol single mass system phenol, derives from tea extract through processing such as decaffeination and recrystallizations, accounts for 25% of tea extraction thing dry weight; Or (Tea Polyphenols TP), accounts for the 60-80% of Tea Polyphenols total amount to derive from Tea Polyphenols.
Contain in catechin and the derivative thereof: Epigallo-catechin gallate (EGCG) (EGCG), nutgall catechin gallic acid ester (GCG), L-Epicatechin gallate (ECG), catechin and gallate (ECG), epigallocatechin (EGC), nutgall catechin (GC), epicatechin (EC).
Catechin involved in the present invention wherein Epigallo-catechin gallate (EGCG) (EGCG) content is not less than 40%, L-Epicatechin gallate (ECG) is not less than 15%, content of caffeine is lower than 2.5%, be a kind of pale yellow powder preparation, easy moisture absorption deliquescence, soluble in water and ethanol, the aqueous solution is faint yellow, subacidity.
Known catechin and derivative thereof have following purposes:
1, be a kind of powerful antioxidant, oxidation resistance is higher than VE 10-20 doubly;
2, various bacteria there is the inhibition ability, some viruses are had resistance;
3, anti-gene mutation;
4, antiallergy;
5, hypoglycemic, reducing blood lipid, diuresis etc.
Therefore, it is anti-oxidant that catechin and derivative thereof are usually used in eliminating free radical, regulates immunologic function, antiallergy, and anti-cancer, anticancer, antibiotic, hypoglycemic blood fat, diuresis delays senility etc.Do not relate to the relevant report of resisting the UV-B ultraviolet radiation.
Summary of the invention
The inventor is through to the research of tea extract catechin, found that catechin can effectively resist the radiation of UV-B to organism.
Avoid the uv b radiation damage with catechin protection organism, or the organism that is subjected to the uv b radiation damage with the catechin maintenance.Epigallo-catechin gallate (EGCG) in the described catechin (EGCG) content is not less than 40%, and L-Epicatechin gallate (ECG) content is not less than 15%, and content of caffeine is lower than 2.5%.The working concentration of described catechin is to contain 100-200 milligram catechin in every liter of solvent; The using method of described catechin is: the method protection that sprays or smear organism before accepting uv b radiation with catechin is by the organism of radiation; after organism suffers the uv b radiation damage, repeatedly spray or smear the method maintenance organism of organism with catechin.
The present invention adopts tea extract through the crystallization preparation of processing such as decaffeination and recrystallization acquisition based on ester catechin, with concentration is that this preparation of 50-500mg/L aqueous solution is material, carry out the anti-UV-B of animals and plants test, prove that this preparation aqueous solution that concentration surpasses 100mg/L damages UV-B very strong defensive ability/resistance ability is promptly arranged.This preparation has strong absorption and shielding action to the radiation of ultraviolet band, and this effect of this preparation is mainly derived from suboxides reduction potential and conjugated double bond.Experiment also proves organism after being subjected to ultraviolet radiation damage, uses this preparation that tangible adjusting and repair are also arranged.
Embodiment
Embodiment one:
After spraying the arabidopsis seedling with this preparation of 100-150mg/L aqueous solution, carry out intensity and be 100 microwatts/minute UV-B (280-320nm) irradiation treatment 2 hours.The result shows that the arabidopsis plant orthostatic that sprays this preparation is good, and blade tension force is strong, does not show the obvious impairment symptom, the contrast that the sprays distilled water symptom that here then occurs significantly withering.The gene activity analysis shows that the arabidopsis chalcone synthase genes (CHS), the phenylalnine ammonialyase gene expression activities such as (PAL) that spray this preparation before the ultra-violet radiation obviously surpass the contrast that sprays distilled water.
UV-B is handled the arabidopsis that obviously damage appears in the back carry out spray treatment with this preparation 100mg/L aqueous solution, and place under normal illumination (3000lux) condition and cultivate, the arabidopsis of this preparation processing grows young leaves, and can normally blossom and bear fruit; The contrast that sprays distilled water then can not be observed the recovery symptom.The processing of this preparation is carried out in explanation before uv b radiation, can effectively prevent the damage of UV-B to body; Carry out this preparation after the uv b radiation damage and handle, also can effectively repair the damage of UV-B body.
Embodiment two:
Catechin is mixed with the aqueous solution that content is 150-200mg/L, or emulsion agent, or gel, is applied to people's daily sun-proof skin care, using method is perhaps smeared the skin of damaged for to smear skin before solar radiation after Exposure to Sunlight.Through and the contrast of not using this preparation crowd, find to use this preparation can significantly suppress the appearance and the melanic formation of skin ultraviolet erythema, can be effectively sun-proof with whiten; This preparation also has tangible after-sun restorative effect simultaneously, and the user does not have any bad reaction.
The present invention proposes to use catechin to resist the radiation of UV-B to organism, because catechin derives from natural plants-tealeaves, tealeaves is the human beings'health drink, without any side effects, therefore, catechin is resisted uv b radiation to people's safety as sunlight screening skin-protecting product, and it is pollution-free to manufacture process, and good prospects for application is arranged.
Claims (2)
1, the purposes of catechin; it is characterized in that: Epigallo-catechin gallate (EGCG) in the described catechin (EGCG) content is not less than 40%; L-Epicatechin gallate (ECG) content is not less than 15%; content of caffeine is lower than 2.5%; avoid the uv b radiation damage with the catechin protection organism that contains mentioned component, or maintenance is subjected to the organism of uv b radiation damage.
2, press the purposes of the described catechin of claim 1; the using method that it is characterized in that described catechin is: the working concentration of described catechin is to contain 100-200 milligram catechin in every liter of solvent; the method protection that sprays or smear organism before accepting uv b radiation with catechin is by the organism of radiation; after organism suffers the uv b radiation damage, repeatedly spray or smear the method maintenance organism of organism with catechin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100892363A CN1316885C (en) | 2004-12-03 | 2004-12-03 | Method for protecting living body in radialization of UV-B using catechuic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100892363A CN1316885C (en) | 2004-12-03 | 2004-12-03 | Method for protecting living body in radialization of UV-B using catechuic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1631140A CN1631140A (en) | 2005-06-29 |
| CN1316885C true CN1316885C (en) | 2007-05-23 |
Family
ID=34847558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100892363A Expired - Fee Related CN1316885C (en) | 2004-12-03 | 2004-12-03 | Method for protecting living body in radialization of UV-B using catechuic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1316885C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839159B (en) * | 2015-04-14 | 2017-01-04 | 河南师范大学 | A kind of Oryza sativa L. defence UV radiation protective |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093528A (en) * | 1993-04-12 | 1994-10-19 | 丁双进 | Health tea |
-
2004
- 2004-12-03 CN CNB2004100892363A patent/CN1316885C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093528A (en) * | 1993-04-12 | 1994-10-19 | 丁双进 | Health tea |
Non-Patent Citations (1)
| Title |
|---|
| EGC-G辐射保护作用的研究 刘官树 姚思德 倪谨 赵芳 孟祥顺，辐射研究与辐射工艺学报，第2期 2002 * |
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| Publication number | Publication date |
|---|---|
| CN1631140A (en) | 2005-06-29 |
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Granted publication date: 20070523 Termination date: 20101203 |