CN1307871C - 肺保存液 - Google Patents
肺保存液 Download PDFInfo
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- CN1307871C CN1307871C CNB2005101042322A CN200510104232A CN1307871C CN 1307871 C CN1307871 C CN 1307871C CN B2005101042322 A CNB2005101042322 A CN B2005101042322A CN 200510104232 A CN200510104232 A CN 200510104232A CN 1307871 C CN1307871 C CN 1307871C
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- preservation
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- 210000004072 lung Anatomy 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 title claims description 13
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 19
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 16
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 12
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 8
- 239000001103 potassium chloride Substances 0.000 claims abstract description 8
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 8
- 239000011780 sodium chloride Substances 0.000 claims abstract description 8
- 230000003204 osmotic effect Effects 0.000 claims abstract description 7
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 6
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims description 9
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 claims description 8
- 229940119744 dextran 40 Drugs 0.000 claims description 7
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 2
- 235000002639 sodium chloride Nutrition 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 210000000056 organ Anatomy 0.000 abstract description 12
- 238000004321 preservation Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 4
- 239000000082 organ preservation Substances 0.000 abstract description 3
- 229920002307 Dextran Polymers 0.000 abstract description 2
- 206010063837 Reperfusion injury Diseases 0.000 abstract description 2
- 238000010171 animal model Methods 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 238000002054 transplantation Methods 0.000 abstract 3
- 208000027418 Wounds and injury Diseases 0.000 abstract 2
- 208000014674 injury Diseases 0.000 abstract 2
- 239000003761 preservation solution Substances 0.000 abstract 2
- 229950008558 ulinastatin Drugs 0.000 abstract 2
- ODVKSTFPQDVPJZ-UHFFFAOYSA-N urinastatin Chemical compound C1C=CCCC11COC(C=2OC=CC=2)OC1 ODVKSTFPQDVPJZ-UHFFFAOYSA-N 0.000 abstract 2
- 108010088854 urinastatin Proteins 0.000 abstract 2
- 230000003064 anti-oxidating effect Effects 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 230000000302 ischemic effect Effects 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 210000002977 intracellular fluid Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004768 organ dysfunction Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明为一种肺保存液，属于一种鲜活器官保存物质。它的成份及含量为：每升含有氯化钠：0.6～1.0g，氯化钾0.02～0.04g，硫酸镁7.0～7.6g，碳酸氢钠0.6～1.0g，右旋糖酐-40：40～60g，乌司他丁25，000～50，000u/kg；渗透压数值范围为300～350mosm/l。本发明以现代器官保存技术理论为基础，结合抗氧化、特殊的渗透作用、细胞交换促进机制，将乌司他丁运用于器官保存方面，用于减少器官移植中的缺血再灌注损伤、延长器官移植的保存时间，减少保存期的损伤，降低移植后原发性无功能的发生率，减少保存损伤而导致的排斥反应，从而从总体上改善器官移植的近期和远期效果。它可以使实验动物离体移植器官的保存时间延长1倍。
Description
本发明为一种肺保存液，属于一种鲜活器官保存物质。
目前进行肺保存的物质一般有细胞内液型保存液和细胞外液型保存液，前者以Euro-collins液(EC液)和University of wisconsin(U液)为主，后者以低钾右旋糖酐液为主。前者对肺的保存效果较差，一般为4～6小时，而且，这两种保存液均为高钾溶液，对肺的损害较大。后者也未见保存时间超过6小时的报道。
本发明的目的在于，公开一种保存效果好的肺保存液。
本发明是由氯化钠、氯化钾、硫酸镁、碳酸氢钠、右旋糖酐-40与水混合而成，其中，每升中各成份的含量为：氯化钠：0.6～1.0g，氯化钾0.02～0.04g，硫酸镁7.0～7.6g，碳酸氢钠0.6～1.0g，右旋糖酐-40：40～60g，乌司他丁：25,000～50,000u/kg；其渗透压的数值范围为300～350mosm/l。本发明中含有乌司他丁，它能够起到抑制组织细胞代谢的作用，使被保存的器官在10℃左右的保存温度下降低代谢酶活性，减少分解代谢，延长组织细胞生命，达到预期的保存器官功能的目的。它以现代器官保存技术理论为基础，结合抗氧化、特殊的渗透作用、细胞交换促进机制，将乌司他丁运用于器官保存方面，用于减少器官移植中的缺血再灌注损伤、延长器官移植的保存时间，减少保存期的损伤，降低移植后原发性无功能的发生率，减少保存损伤而导致的排斥反应，从而从总体上改善器官移植的近期和远期效果。它可以使实验动物离体移植器官的保存时间延长1倍。在具体生产时，组成本发明的各成份可采用以下具体数据：每升中各成份的含量为：氯化钠：0.8～0.9g，氯化钾0.02～0.04g，硫酸镁7.2～7.5g，碳酸氢钠0.8～0.9g，右旋糖酐-40：45～50g，乌司他丁30,000～40,000u/kg，渗透压的数值范围为310～330mosm/l。
具体实施方式
各实施例中每升溶液中各成份的含量如下：
| 参数 | 成份 | 实施例1 | 实施例2 | 实施例3 | 实施例4 | 实施例5 | 实施例6 | 实施例7 | 实施例8 | 实施例9 |
| 氯化钠(g) | 0.6 | 0.65 | 0.7 | 0.75 | 0.8 | 0.85 | 0.9 | 0.95 | 1.0 | |
| 氯化钾(g) | 0.02 | 0.03 | 0.04 | 0.025 | 0.035 | 0.04 | 0.02 | 0.03 | 0.035 | |
| 硫酸镁(g) | 7.35 | 7.4 | 7.0 | 7.3 | 7.4 | 7.6 | 7.2 | 7.1 | 7.5 | |
| 碳酸氢钠(g) | 0.7 | 0.65 | 0.9 | 0.6 | 0.8 | 0.85 | 1.0 | 0.95 | 0.75 | |
| 右旋糖酐-40(g) | 45 | 53 | 50 | 47 | 40 | 43 | 60 | 55 | 57 | |
| 渗透压(mosm/l) | 310 | 345 | 320 | 315 | 330 | 335 | 340 | 325 | 350 | |
| 乌司他丁(u/kg) | 30000 | 48000 | 40000 | 25000 | 32000 | 37000 | 50000 | 42000 | 38000 |
上述各实施例中的成份在常温下混合制成本发明。按上述成份制成的本发明其渗透压参数不在要求范围之内时，按公知方法对其进行调整。
Claims (2)
1、一种肺保存液，其特征是：它是由氯化钠、氯化钾、硫酸镁、碳酸氢钠、右旋糖酐-40与水混合而成，其中，每升中各成份的含量为：氯化钠：0.6～1.0g，氯化钾：0.02～0.04g，硫酸镁：7.0～7.6g，碳酸氢钠：0.6～1.0g，右旋糖酐-40：40～60g，乌司他丁：25,000～50,000u/kg；其渗透压的数值范围为300～350mosm/l。
2、根据权利要求1所述的肺保存液，其特征是：每升中各成份的含量为：氯化钠：0.8～0.9g，氯化钾：0.02～0.04g，硫酸镁：7.2～7.5g，碳酸氢钠：0.8～0.9g，右旋糖酐-40：45～50g，乌司他丁30,000～40,000u/kg；渗透压的数值范围为310～330mosm/l。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101042322A CN1307871C (zh) | 2005-10-11 | 2005-10-11 | 肺保存液 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101042322A CN1307871C (zh) | 2005-10-11 | 2005-10-11 | 肺保存液 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1748499A CN1748499A (zh) | 2006-03-22 |
| CN1307871C true CN1307871C (zh) | 2007-04-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101042322A Expired - Fee Related CN1307871C (zh) | 2005-10-11 | 2005-10-11 | 肺保存液 |
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| Country | Link |
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| CN (1) | CN1307871C (zh) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803594B (zh) * | 2009-02-14 | 2013-07-31 | 青岛华仁药业股份有限公司 | 一种器官保存液及其制备方法 |
| ES2913104T3 (es) * | 2011-04-14 | 2022-05-31 | Transmedics Inc | Solución de cuidado de órganos para perfusión en máquina ex-vivo de pulmones de donantes |
| CN103461322B (zh) * | 2012-06-07 | 2016-08-03 | 西比曼生物科技（上海）有限公司 | 用于冻存复苏后的细胞的保存液 |
| CN112273373B (zh) * | 2020-10-29 | 2022-06-07 | 无锡市人民医院 | 离体肺脏机械灌注液及其制备方法和应用 |
| CN113498777B (zh) * | 2021-07-26 | 2022-12-30 | 公卫复医(上海)生物科技有限公司 | 一种乳剂型器官保存液及其制备方法 |
-
2005
- 2005-10-11 CN CNB2005101042322A patent/CN1307871C/zh not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 供肺保护的研究进展 陈新忠 孙宇全，中华胸心血管外科杂志，第20卷第5期 2004 * |
| 供肺保护的研究进展 陈新忠 孙宇全，中华胸心血管外科杂志，第20卷第5期 2004;移植肺保护的研究进展 黄戈，医学研究生学报，第17卷第5期 2004 * |
| 移植肺保护的研究进展 黄戈，医学研究生学报，第17卷第5期 2004 * |
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| Publication number | Publication date |
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| CN1748499A (zh) | 2006-03-22 |
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