CN1302005C - 硫代磷酸寡核苷酸的制备方法 - Google Patents

硫代磷酸寡核苷酸的制备方法 Download PDF

Info

Publication number
CN1302005C
CN1302005C CNB018201016A CN01820101A CN1302005C CN 1302005 C CN1302005 C CN 1302005C CN B018201016 A CNB018201016 A CN B018201016A CN 01820101 A CN01820101 A CN 01820101A CN 1302005 C CN1302005 C CN 1302005C
Authority
CN
China
Prior art keywords
oligonucleotide
support
oligonucleotides
solid support
acetonitrile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB018201016A
Other languages
English (en)
Chinese (zh)
Other versions
CN1479745A (zh
Inventor
M·E·杜格拉斯
B·J·梅洛
D·A·维林斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Avecia Inc
Original Assignee
EFFSIA Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0029610A external-priority patent/GB0029610D0/en
Priority claimed from US09/740,031 external-priority patent/US6768005B2/en
Application filed by EFFSIA Co Ltd filed Critical EFFSIA Co Ltd
Publication of CN1479745A publication Critical patent/CN1479745A/zh
Application granted granted Critical
Publication of CN1302005C publication Critical patent/CN1302005C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
CNB018201016A 2000-12-05 2001-12-03 硫代磷酸寡核苷酸的制备方法 Expired - Fee Related CN1302005C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0029610.3 2000-12-05
GB0029610A GB0029610D0 (en) 2000-12-05 2000-12-05 Process
US09/740,031 2000-12-20
US09/740,031 US6768005B2 (en) 2000-12-20 2000-12-20 Process

Publications (2)

Publication Number Publication Date
CN1479745A CN1479745A (zh) 2004-03-03
CN1302005C true CN1302005C (zh) 2007-02-28

Family

ID=26245377

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB018201016A Expired - Fee Related CN1302005C (zh) 2000-12-05 2001-12-03 硫代磷酸寡核苷酸的制备方法

Country Status (8)

Country Link
US (1) US7227017B2 (cg-RX-API-DMAC7.html)
EP (2) EP2332954A3 (cg-RX-API-DMAC7.html)
JP (1) JP2004517089A (cg-RX-API-DMAC7.html)
KR (1) KR20040016826A (cg-RX-API-DMAC7.html)
CN (1) CN1302005C (cg-RX-API-DMAC7.html)
AU (1) AU2002220870A1 (cg-RX-API-DMAC7.html)
CA (2) CA2437040C (cg-RX-API-DMAC7.html)
WO (1) WO2002046205A2 (cg-RX-API-DMAC7.html)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0229443D0 (en) 2002-12-18 2003-01-22 Avecia Ltd Process
EP1660512A4 (en) * 2003-06-02 2009-12-23 Isis Pharmaceuticals Inc SYNTHESIS OF OLIGONUCLEOTIDES USING ALTERNATIVE SOLVENTS
EP2205619A4 (en) * 2007-10-15 2013-04-17 Honeywell Int Inc SYNTHESIS OF OLIGONUCLEOTIDES OR A PHOSPHOROTHIOATE OLIGONUCLEOTIDE WITH A 1,3,5-TRIMETHYLHEXAHYDRO-1,3,5-TRIAZINE-FREE N-METHYLIMIDAZOLE STRIPPING AGENT
WO2012059510A1 (en) * 2010-11-02 2012-05-10 Girindus America, Inc. Back pressure control during solid-phase synthesis on polymeric supports
EP2885312B1 (en) * 2012-08-15 2025-09-03 Ionis Pharmaceuticals, Inc. Method of preparing oligomeric compounds using modified capping protocols
KR102207770B1 (ko) 2013-08-05 2021-01-26 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation NOVO SYNTHESIZED NUCLEIC ACID BANKS
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3586255B1 (en) 2017-02-22 2025-01-15 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物
IL275818B2 (en) 2018-01-04 2024-10-01 Twist Bioscience Corp Dna-based digital information storage
IL278771B2 (en) 2018-05-18 2025-09-01 Twist Bioscience Corp Polynucleotides, reagents and methods for nucleic acid hybridization
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGHLY ACCURATE POLYNUCLEOTIDE DE NOVO SYNTHESIS
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
WO2021061829A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
MX2023007242A (es) * 2020-12-18 2023-06-29 Lilly Co Eli Sintetizador de oligonucleotidos de lecho fluidizado oscilante.
CN114573652B (zh) * 2022-03-15 2025-02-07 安徽瑞拜药业有限公司 药用核酸合成工艺
CN117563009B (zh) 2023-09-01 2025-07-01 杭州天龙药业有限公司 一种含有核糖环或其衍生结构的GalNAc化合物及其寡核苷酸缀合物

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001446A2 (en) * 1992-07-09 1994-01-20 Beckman Instruments, Inc. Derivatized organic solid support for nucleic acid synthesis

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5132418A (en) * 1980-02-29 1992-07-21 University Patents, Inc. Process for preparing polynucleotides
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4500707A (en) * 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US4973679A (en) * 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
CA1206457A (en) * 1981-10-07 1986-06-24 Alan Rosevear Synthesis of compounds
USRE34069E (en) * 1983-08-18 1992-09-15 Biosyntech Gmbh Process for the preparation of oligonucleotides
US5362866A (en) * 1983-09-02 1994-11-08 Molecular Biosystems, Inc. Oligonucleotide polymeric support system with an oxidation cleavable link
US4795700A (en) * 1985-01-25 1989-01-03 California Institute Of Technology Nucleic acid probes and methods of using same
EP0216357A3 (en) * 1985-09-25 1988-08-31 Nippon Zeon Co., Ltd. Phosphoramidite compounds and process for production thereof
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
CA1315968C (en) * 1987-04-24 1993-04-13 David Colin Sherrington Substrate and process for making a substrate
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0291088A (ja) * 1988-09-29 1990-03-30 Central Glass Co Ltd ホスファイトおよびヌクレオシド‐３’‐ホスファイト誘導体およびこれを用いるオリゴヌクレオチドの合成法
US5047524A (en) * 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5164491A (en) * 1989-06-15 1992-11-17 Gilead Sciences Large scale synthesis of oligonucleotides and their associated analogs
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
EP0625214A4 (en) * 1991-12-23 1997-07-16 Chiron Corp Cmv probes for use in solution phase sandwich hybridization assays.
US5614621A (en) * 1993-07-29 1997-03-25 Isis Pharmaceuticals, Inc. Process for preparing oligonucleotides using silyl-containing diamino phosphorous reagents
US5514789A (en) * 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
US5554746A (en) * 1994-05-16 1996-09-10 Isis Pharmaceuticals, Inc. Lactam nucleic acids
US5510476A (en) * 1994-07-07 1996-04-23 Isis Pharmaceuticals, Inc. Carbocation scavenging during oligonucleotide synthesis
US5705621A (en) * 1995-11-17 1998-01-06 Isis Pharmaceuticals, Inc. Oligomeric phosphite, phosphodiester, Phosphorothioate and phosphorodithioate compounds and intermediates for preparing same
US5869696A (en) * 1996-04-22 1999-02-09 Beckman Instruments, Inc. Universal solid supports and methods for their use
US6096881A (en) * 1997-05-30 2000-08-01 Hybridon, Inc. Sulfur transfer reagents for oligonucleotide synthesis
US6111086A (en) * 1998-02-27 2000-08-29 Scaringe; Stephen A. Orthoester protecting groups
US6069243A (en) * 1998-10-06 2000-05-30 Isis Pharmaceuticals, Inc. Process for oligonucleotide synthesis
US6465628B1 (en) 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
JP4705716B2 (ja) 1999-02-05 2011-06-22 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション オリゴヌクレオチドの脱保護法
GB9924285D0 (en) * 1999-10-14 1999-12-15 Avecia Ltd Process
EP1294736A1 (en) * 2000-06-12 2003-03-26 Avecia Biotechnology, Inc. Deprotection of synthetic oligonucleotides using an acrylonitrile scavenger
US6768005B2 (en) * 2000-12-20 2004-07-27 Avecia Limited Process
JP4348044B2 (ja) * 2002-02-12 2009-10-21 株式会社キラルジェン 立体規則性の高いジヌクレオシドホスホロチオエートの製造法
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001446A2 (en) * 1992-07-09 1994-01-20 Beckman Instruments, Inc. Derivatized organic solid support for nucleic acid synthesis

Also Published As

Publication number Publication date
US20030229221A1 (en) 2003-12-11
WO2002046205A2 (en) 2002-06-13
JP2004517089A (ja) 2004-06-10
EP1343802B1 (en) 2012-05-30
KR20040016826A (ko) 2004-02-25
CA2714353A1 (en) 2002-06-13
CA2437040C (en) 2011-01-25
US7227017B2 (en) 2007-06-05
EP1343802A2 (en) 2003-09-17
CA2437040A1 (en) 2002-06-13
EP2332954A2 (en) 2011-06-15
AU2002220870A1 (en) 2002-06-18
CN1479745A (zh) 2004-03-03
WO2002046205A3 (en) 2002-08-15
EP2332954A3 (en) 2011-12-21

Similar Documents

Publication Publication Date Title
CN1302005C (zh) 硫代磷酸寡核苷酸的制备方法
US6768005B2 (en) Process
EP0219342B1 (en) Method and reagents for in vitro oligonucleotide synthesis
JP3081882B2 (ja) 還元によるヒドロキシル保護基の直交除去及びオリゴヌクレオチドの化学合成におけるそれらの利用
KR101032008B1 (ko) 폴리뉴클레오티드 표지 시약
EP0843684B1 (en) Universal solid supports and methods for their use
AU725627B2 (en) Reusable solid support for oligonucleotide synthesis, process for production thereof and process for use thereof
US20240209018A1 (en) Universal linker reagents for dna synthesis
CN1262557C (zh) 制备硫代磷酸三酯的方法
CN1409719A (zh) 制备硫代磷酸三酯和寡核苷酸的方法
AU2022291626A1 (en) Improved process for preparing imetelstat
US7098326B2 (en) Methods for the integrated synthesis and purification of oligonucleotides
CN1444595A (zh) 利用丙烯腈清除剂去保护合成的寡核苷酸
US20040039189A1 (en) Novel amidite derivates for synthesising polymers on surfaces
JP2011517695A (ja) ５’−リン酸モノエステル又は５’−チオリン酸モノエステル末端を含むオリゴヌクレオチドの新規製造方法
HK40026576A (en) Improved process for preparing imetelstat
WO2003045969A1 (en) Coupling reagent for h-phosphonate chemistry
HK40031465B (en) Improved process for preparing imetelstat

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
ASS Succession or assignment of patent right

Owner name: SUSHE CHEMICAL TECHNOLOGY CO., LTD.

Free format text: FORMER OWNER: AVECIA LTD.

Effective date: 20070511

C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20070511

Address after: Massachusetts

Patentee after: Avecia Biotechnology Inc.

Address before: University of Manchester

Patentee before: Effsia Co., Ltd.

C19 Lapse of patent right due to non-payment of the annual fee
CF01 Termination of patent right due to non-payment of annual fee