CN1297739A - Technological process of extracting antineoplastic active component from shark cartilage - Google Patents

Abstract

The technological process includes mixing crushed shark cartilage into 0.5-5% concentration water solution of basic salt in the amount of 2-15 times that of the shark cartilage weight, homogenizing or grinding the mixture in a homogenizer, a grinder or a colloidal mill to 20 mesh below fineness, centrifugalizing to separate liquid from dregs, and ultrafiltering or nanometer filtering the liquid to concentrate and to reserve the matter with molecular weight over 500 dalton with all the operation being performed at temperature lower than 60 deg.c. The process of the present invention can maintain the activity of the effective components in shark cartilage and raise the yield of the product.

Description

The extraction process of anti-tumor active ingredient in the shark cartilage

The present invention relates to from shark cartilage, extract the technology of anti-tumor active ingredient.

The composition that contains neutral sugar, acid mucopolysaccharide, chondroitin sulfate, protein and plurality of enzymes in the shark cartilage, the active component that contains the inhibition new vessels generation of high concentration in the extract of shark cartilage, have the neoplasm growth effect, thereby be usually used in tumor treatment.The research of active component has a large amount of reports in the relevant shark cartilage, the extraction of effective ingredient also has many methods in the shark cartilage, (this Shen is flourish earlier, the shark cartilage preparation Antitumor Effects of Jia Fuxing such as INM, be published in the academic exploitation of Chinese ocean lakes and marhshes medicine Conference Papers collection volume two the 4th time, Yantai, 1994.4) be raw material with the shark cartilage, through the hydrochloric acid extracting, steps such as acetone fractional precipitation, ultrafiltration, column chromatography make the shark cartilage preparation, through mouse animal experiment, said preparation has the activity that stronger inhibition tumor generates; The patent of this experiment Tener company of Canadian for another example Lay (E. Du Pont, P. Bradley pool is difficult to understand, and C. Zhu Niao has shark cartilage extract of anti-angiogenesis activity and effect on tumor regression and preparation method thereof; 97 years CN 1153477 of Chinese patent) in, shark cartilage is added mixing, centrifugal in the neutral water, to obtain shark cartilage extract after the supernatant lyophilizing, this extract has the activity that angiogenesis inhibitor characteristic and in-vivo tumour disappear, and tumor cell is shown direct inhibitory action, these extracting method are many to extract certain or certain constituents is a purpose, and the production yield is lower.

Because the shark cartilage expensive raw material price, in big production, need to consider the production yield, and keeping its active component to greatest extent, the object of the present invention is to provide a kind of new technology that the shark cartilage composition is extracted, higher yield can be arranged.

Technical scheme of the present invention is achieved in that the extraction process of anti-tumor active ingredient in the shark cartilage, it comprises that mainly shark cartilage is pulverized the back to be mixed, even again oar or grinding, extract through stirring then, centrifugalize, concentrate, lyophilizing, pulverize, preservation after sieving, it is characterized in that: the shark cartilage after will pulverizing join by weight 1: 2-1: in 15 the basic salt aqueous solution, the weight concentration of this basic salt aqueous solution is 0.5-5%, even oar or grinding are that mixture with cartilage slag and the formation of basic salt solution is at even oar device, fully disperse in grinder or the colloid mill, reach the following fineness of 20 orders; Centrifugalize is that the mixture after stir extracting is centrifugal with solid and liquid separately by frozen centrifugation or room temperature, concentrate is that liquid with centrifugalize bone slag gained concentrates by ultrafiltration or nanofiltration means, keep molecular weight at the above material of 500 dalton, all operations all carries out under the temperature that is lower than 60 degree.

The present invention is owing to the shark cartilage after will pulverizing joins in the basic salt, and the corresponding even oar of employing or grinding and concentrated means, and shark cartilage is extracted, and both can keep the activity of these compositions, has ensured higher yield again.

Below the invention will be further described and provide embodiment.

The present invention comprises that mainly shark cartilage is pulverized the back to be mixed, even again oar or grinding, extract through stirring then, centrifugalize, concentrate, lyophilizing, pulverize, preservation after sieving, it is characterized in that: the shark cartilage after will pulverizing join by weight 1: 2-1: in 15 the basic salt aqueous solution, the weight concentration of the aqueous solution of this basic salt is 0.5-5%, following concentration is weight concentration, also can be with bone meal, basic salt is added in the water after mixing, make the concentration of this basic salt reach 0.5-5%, basic salt comprises sodium, the carbonate of potassium, subphosphate and acylate, subphosphate is sodium hydrogen phosphate and dipotassium hydrogen phosphate in an embodiment, and acylate is an acetic acid, the sodium salt of propanoic acid and citric acid and potassium salt; Even oar or grinding are that the mixture of cartilage slag with the formation of basic salt solution fully disperseed in even oar device, grinder or colloid mill, and the fineness that makes the bone slag is less than 5 μ m; Stir to extract and generally in stirring reactor, carry out, need to stir 2-12 hour, centrifugalize is that the mixture after stir extracting is centrifugal with solid and liquid separately by frozen centrifugation or room temperature, concentrate is that liquid with centrifugalize bone slag gained concentrates by ultrafiltration (molecular retention amount 4000 dalton) or nanofiltration (molecular weight interception 300 dalton) method, the volume cocnentration factor is 2: 1-10: 1, following cocnentration factor is the volume cocnentration factor, adopt frozen vacuum dryer that concentrated solution is carried out lyophilization again, temperature is that negative 50 degree Celsius are to negative 10 degree, below be degree centigrade, can obtain the activity extract of shark cartilage, all operations all carries out under the temperature that is lower than 60 degree, the extract that adopts the present invention to obtain according to check contains shark cartilage, acid mucopolysaccharide, neutral sugar and protein isoreactivity composition, can be water-soluble, be easy to absorb, without any side effects, to cancerous cell (hepatocarcinoma, cervical cancers etc.) the synthetic cell proliferation that reaches of the DNA of vascular endothelial cell has obvious inhibition, to solid tumor (pulmonary carcinoma, osteosarcoma etc.) suppression ratio is 40-70%, simultaneously owing to contain mucopolysaccharide, the effect that also has the human body immunity improving function.

Embodiment 1

20 kilograms of shark cartilage are crushed to 20 orders, join 200 kilograms of concentration and be in 1% the sodium carbonate liquor and mix, mixture is obtained feed liquid with the even oar of even oar device to＜5 μ m, again this feed liquid is added in the agitator and stirs, temperature is room temperature 25 degree, mixing time 6 hours, adopt refrigerated centrifuger then under 4 degree, carry out solid-liquid separation, obtain supernatant, the reuse ultrafilter is with this supernatant concentration, cocnentration factor is 1: 0.3, and the molecular retention amount is 4000 dalton, again after negative 40 degree lyophilisation, get 3.21 kilograms of extracts, product yield 16%.

Embodiment 2

10 kilograms of shark cartilage are crushed to 20 orders, join 130 kilograms of concentration and be in 1.5% the liquor kalii acetici and mix, adopt colloid mill that the bone slag is ground, and make it abundant dispersion, this feed liquid is added in the agitator stirs, at room temperature stirred 12 hours, again after centrifuge at room temperature carries out solid-liquid separation, obtain supernatant, adopt collecting and filtering apparatus that supernatant is concentrated, cocnentration factor 8: 1 is with concentrated solution lyophilisation under negative 40 degree, can obtain 1.8 kilograms extract at last, yield is 18%.

Embodiment 3

10 kilograms of shark cartilage are pulverized, join in 80 kilogram 0.5% the sodium hydrogen phosphate aqueous solution, fully mix to obtain slip with grinder with bone slag porphyrize and with aqueous solution, this slip is added in the agitator tank, stirred 3 hours under the room temperature, is to carry out solid-liquid separation under 4 degree to obtain supernatant with refrigerated centrifuger in temperature, the reuse collecting and filtering apparatus is with supernatant concentration, and being concentrated into cocnentration factor is 2: 1, with concentrated solution lyophilisation under negative 30 degree, get 1.72 kilograms of extracts, yield is 17.2%.

Embodiment 4

20 kilograms of shark cartilage are pulverized, join 240 kilograms of concentration and be in 4.5% the sodium acetate solution, adopt colloid mill to grind the bone slag and make it fully to mix to obtain slip, this slip is added in the agitator, at room temperature stirred 2 hours with aqueous solution, the reuse refrigerated centrifuger carries out centrifugalize and obtains supernatant under 3 degree, then with collecting and filtering apparatus with supernatant concentration, cocnentration factor is 5: 1, with concentrated solution lyophilisation under negative 40 degree, get 3.56 kilograms of extracts, yield is 17.8%.

Embodiment 5

Press the method for embodiment 2, adopting concentration is that 1.5% solution of potassium carbonate carries out active component to shark cartilage and extracts, 1.88 kilograms of extracts, yield 18.8%.

Claims (3)

1, the extraction process of anti-tumor active ingredient in the shark cartilage, it comprises that mainly shark cartilage is pulverized the back to be mixed, even again oar or grinding, then through stir extraction, centrifugalize, concentrate, lyophilizing, pulverize, sieve after preservation, it is characterized in that: the shark cartilage after will pulverizing join by weight 1: 2-1: in 15 the basic salt aqueous solution, the concentration of this basic salt aqueous solution is 0.5-5%, even oar or grinding are that cartilage slag and the mixture that basic salt solution forms are fully disperseed in even oar device, grinder or colloid mill, reach the fineness below 20 orders; Centrifugalize is that the mixture after stir extracting is centrifugal with solid and liquid separately by frozen centrifugation or room temperature, concentrate is that liquid with centrifugalize bone slag gained concentrates by ultrafiltration or nanofiltration means, keep molecular weight at the above material of 500 dalton, all operations all carries out under the temperature that is lower than 60 degree.

2, by the extraction process of anti-tumor active ingredient in the described shark cartilage of claim 1, it is characterized in that: basic salt comprises the carbonate of sodium, potassium, subphosphate and acylate.

3, by the extraction process of anti-tumor active ingredient in the described shark cartilage of claim 1, it is characterized in that: subphosphate is sodium hydrogen phosphate and dipotassium hydrogen phosphate, and acylate is the sodium salt and the potassium salt of acetic acid, propanoic acid and citric acid.