CN1295329C - 制备dna接头的方法 - Google Patents
制备dna接头的方法 Download PDFInfo
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- CN1295329C CN1295329C CNB2004100656799A CN200410065679A CN1295329C CN 1295329 C CN1295329 C CN 1295329C CN B2004100656799 A CNB2004100656799 A CN B2004100656799A CN 200410065679 A CN200410065679 A CN 200410065679A CN 1295329 C CN1295329 C CN 1295329C
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- 238000000034 method Methods 0.000 title claims abstract description 27
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- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 238000013461 design Methods 0.000 claims abstract description 6
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 claims abstract 2
- 230000008859 change Effects 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 14
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 claims description 10
- 239000012634 fragment Substances 0.000 claims description 10
- 238000004153 renaturation Methods 0.000 claims description 10
- 230000003321 amplification Effects 0.000 claims description 8
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 8
- 238000005520 cutting process Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 108010042407 Endonucleases Proteins 0.000 claims description 4
- 102000004533 Endonucleases Human genes 0.000 claims description 4
- 230000029087 digestion Effects 0.000 claims description 4
- 238000009396 hybridization Methods 0.000 claims description 4
- 230000008676 import Effects 0.000 claims description 4
- 239000012451 post-reaction mixture Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000001976 enzyme digestion Methods 0.000 abstract 4
- 101100182721 Mus musculus Ly6e gene Proteins 0.000 abstract 1
- 230000001851 biosynthetic effect Effects 0.000 abstract 1
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- 238000000926 separation method Methods 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 15
- 238000002360 preparation method Methods 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 238000013467 fragmentation Methods 0.000 description 3
- 238000006062 fragmentation reaction Methods 0.000 description 3
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 3
- 238000010189 synthetic method Methods 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 238000007852 inverse PCR Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000001308 synthesis method Methods 0.000 description 2
- 108010005054 Deoxyribonuclease BamHI Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
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Abstract
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Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2004100656799A CN1295329C (zh) | 2004-11-12 | 2004-11-12 | 制备dna接头的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CNB2004100656799A CN1295329C (zh) | 2004-11-12 | 2004-11-12 | 制备dna接头的方法 |
Publications (2)
Publication Number | Publication Date |
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CN1618962A CN1618962A (zh) | 2005-05-25 |
CN1295329C true CN1295329C (zh) | 2007-01-17 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CNB2004100656799A Active CN1295329C (zh) | 2004-11-12 | 2004-11-12 | 制备dna接头的方法 |
Country Status (1)
Country | Link |
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CN (1) | CN1295329C (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101037685B (zh) * | 2007-02-09 | 2010-05-19 | 南京大学 | 一种天然aflp接头制备的方法 |
CN102061335B (zh) * | 2010-11-15 | 2014-07-23 | 苏州众信生物技术有限公司 | 一种二代高通量测序的不对称dna双链接头及其应用 |
CN111718961B (zh) * | 2019-07-03 | 2022-02-08 | 华大青兰生物科技(无锡)有限公司 | 一种用质粒转化细菌的方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995025538A1 (en) * | 1994-03-18 | 1995-09-28 | The General Hospital Corporation | Cleaved amplified rflp detection methods |
CN1133344A (zh) * | 1995-04-12 | 1996-10-16 | 夏庆杰 | 万能dna片段扩增技术及万能dna片段扩增试剂盒 |
CN1422962A (zh) * | 2001-12-06 | 2003-06-11 | 中南大学 | 生物品种基因组dna指纹图谱 |
CN1461809A (zh) * | 2002-05-31 | 2003-12-17 | 中国人民解放军基因工程研究所 | 一种限制性扩增方法 |
-
2004
- 2004-11-12 CN CNB2004100656799A patent/CN1295329C/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995025538A1 (en) * | 1994-03-18 | 1995-09-28 | The General Hospital Corporation | Cleaved amplified rflp detection methods |
CN1133344A (zh) * | 1995-04-12 | 1996-10-16 | 夏庆杰 | 万能dna片段扩增技术及万能dna片段扩增试剂盒 |
CN1422962A (zh) * | 2001-12-06 | 2003-06-11 | 中南大学 | 生物品种基因组dna指纹图谱 |
CN1461809A (zh) * | 2002-05-31 | 2003-12-17 | 中国人民解放军基因工程研究所 | 一种限制性扩增方法 |
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Publication number | Publication date |
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CN1618962A (zh) | 2005-05-25 |
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