CN1274535A - Anti-oxidation process for natural bee glue - Google Patents
Anti-oxidation process for natural bee glue Download PDFInfo
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- CN1274535A CN1274535A CN00114519A CN00114519A CN1274535A CN 1274535 A CN1274535 A CN 1274535A CN 00114519 A CN00114519 A CN 00114519A CN 00114519 A CN00114519 A CN 00114519A CN 1274535 A CN1274535 A CN 1274535A
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- bee glue
- propolis
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- oxidation process
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention aims at improving the anti-oxidation ability of bee glue. By means of the advanced technological processes of enzyme-promoted controlled decomposition, supercritical solvent concentration and extraction, ion exchange resin adsorption and elution, low-temperature film evaporation and concentration, vacuum cooling and drying, etc., bee glue has its various effective components maintained and its anti-oxidation ability raised greatly. The bee glue may be used widely as natural antioxidant in food, medicine, daily use chemical and other products.
Description
The present invention relates to the anti-oxidant technology of propolis.
The propolis resin that to be honeybee gather from positions such as the tree bud of plant, barks, a kind of colloid substance that mixes secretion again, is transformed through honeybee processing with honeybee lingual gland, wax gland body.At present, domestic propolis product mostly is to take water-soluble or alcohol extract, method is simple, the active material of multiple effective ingredient contained in the propolis is run off in a large number, cause effective component content low, harmful heavy metal and other impurity content are higher, make its oxidation resistance relatively poor, easily oxidation deterioration.
The object of the present invention is to provide a kind of process of oxidation resistance of propolis, make its propolis product be difficult for oxidation deterioration, improve the content of its effective ingredient.
The object of the present invention is achieved like this: its main technique method is: (1) is carried out enzymatic control and is decomposed after the raw material sorting, promptly select for use biology enzyme under 20～30 ℃ of temperature, pH value 6.0～6.5 and enzyme solvent medium to propolis in the controlled decomposition of biopolymer tissue; (2) adopt the mode of adding 5% natural polymer Surfactant, realize the overcritical concentration extraction of propolis effective ingredient in pure aqueous solvent; (3) carry out the ultrahigh speed centrifugation; (4) carry out ion exchange resin absorption and wash-out; (5) eluent is carried out the cryogenic film evaporation and concentration; (6) carry out vacuum freeze drying; (7) carry out composite and the vacuum dehumidifying.
Above-mentioned said enzymatic control is decomposed, and the optimum value of its temperature is 25 ℃.
The invention has the advantages that the oxidation resistance that has greatly improved propolis product, make more than 200 kind of effective ingredient in the propolis keep getting off intactly, the fresh aquatic food material (food, medicine, drink etc.) that makes the high protein in being suitable for is just rotten and extend to more than 2 years by depositing 2～4 hours originally.And make that to mainly contain effective constituent (as materials such as 5.7-dihydroxy-3,4-dimethyl flavones and 5-hydroxyl-4,7-dimethyl flavanones) performance very stable, never degenerate.The effective component content of propolis is higher than national standard (50～75%) far away up to 72.5%～92.5%.Content of beary metal in the propolis is 0.55PPm only, than low 20 times of national standard (10PPm).The present invention can make it not add any leavening agent under 38 ℃ of high temperature and auxiliary material all can not bond, and the active material of more than 200 kind of effective ingredient is not run off, oxidation deterioration not, therefore, the present invention can be widely used in products such as food, health food, drink with function, medicine, daily-use chemical industry as natural antioxidant, has very wide application value and market prospects.
Below the present invention is further described.
Through the applicant for many years repeatedly experiment show: propolis has very strong oxidation resistance under the concentration of 0.01～0.08% (optimum value 0.05%), than anti-oxidation active substance Senior Two such as the dibutyl hydroxy toluene of equivalent, gallic acid third lipoprotein doubly more than.So high oxidation resistance derives from and has adopted a whole set of advanced process means in the production.Its technology is: the raw material sorting → overcritical concentration extraction of enzymatic control decomposition → solvent → ultrahigh speed centrifugation → ion exchange resin absorption and wash-out → cryogenic film evaporation and concentration → vacuum freeze drying → composite → vacuum dehumidifying → packing.Wherein, relatively more crucial technology content is:
(1) enzymatic control decomposing organism engineering.
The basic proterties of propolis is black or bronze-colored block, the smooth densification of section, and the chance thermal change is soft, and viscosity is extremely strong.Therefore, be difficult to extract effective ingredient with commonsense method.The present invention has added enzymatic control decomposition process before solvent extraction, promptly select the special highly single-minded biology enzyme that has for use, under 20～30 ℃ of temperature (best 25 ℃), pH value 6.0～6.5 and enzyme solvent medium to propolis in the controlled decomposition of biopolymer tissue.
(2) overcritical concentration solvent extraction.
The maximum concentration that predetermined substance can reach in specific solvent is called critical concentration and saturated concentration, for concrete material, should be a constant.But it is unfavorable that this characteristic brings for pure aqueous solvent extraction propolis, and limited because of its concentration that can reach, dissolution efficiency is lower.The present invention adopts the mode of adding 5% natural polymer Surfactant, has realized that propolis has the overcritical concentration of composition in pure aqueous solvent to extract, and the stripping extraction efficiency improves greatly.
(3) ion exchange resin absorption and wash-out.
Ion exchange resin absorption and elution technique are one of modern key technologies of herbal pharmaceutical in recent years.The present invention introduces the method, and the rejecting of the collection of propolis effective ingredient, invalid element, energy-conservation and environmental protection etc. all are of great importance.
(4) cryogenic film evaporation and concentration.
Eluent after the resin adsorption adopts normal-temperature distilled concentrated, decompression distillation concentration technique usually before dry.The present invention adopts hollow membrane pervaporation concentration technique, has realized that low temperature truly concentrates, and is very favourable to the preservation of propolis effective ingredient active material, and energy savings.
(5) vacuum freeze drying.
Vacuum Freezing ﹠ Drying Technology be until the eighties not the phase really obtain thousand dry technology of extensive use in the world, also be up to now till best dry technology.But have relatively high expectations, so bee product processing industry and extensively popularization of end acquisition at home because of technical.The present invention takes the lead in introducing this technology in the propolis processing industry.Its basic principle is under freezing state, and material contained humidity forms ice crystal (molecular crystal), and this ice crystal surface has the gas phase of an extremely low air pressure and the balance of solid phase; If system is vacuumized, keep a pressure littler than this water vapor pressure, then hydrone is constantly taken away through gas form from the solid ice crystal surface, until draining.Whole process shows as a complete sublimation process, has avoided the destruction of ice crystal liquefaction to the material form, and the product quality is fluffy, the color fidelity, and mouthfeel is fabulous.Simultaneously, vacuum freeze drying is in low-temperature condition because of system, avoided the hot blast spraying and directly in the vacuum drying heat energy to the influence of material form, quality, color effective ingredients such as flavonoids, terpenes, glucoside, phenols are not compromised, do not run off.
Claims (2)
1, a kind of anti-oxidation process for natural bee glue, it is characterized in that: (1) is carried out enzymatic control and is decomposed after the raw material sorting, promptly select biology enzyme for use, under 20～30 ℃ of temperature, pH value 6.0～6.5 and enzyme solvent medium to propolis in the controlled decomposition of biopolymer tissue; (2) adopt the mode of adding 5% natural polymer Surfactant, realize the overcritical concentration extraction of propolis effective ingredient in pure aqueous solvent; (3) carry out the ultrahigh speed centrifugation; (4) carry out ion exchange resin absorption and wash-out; (5) eluent is carried out the cryogenic film evaporation and concentration; (6) carry out vacuum freeze drying; (7) carry out composite and the vacuum dehumidifying.
2, anti-oxidation process for natural bee glue according to claim 1 is characterized in that: above-mentioned said enzymatic control is decomposed, and the optimum value of its temperature is 25 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00114519A CN1274535A (en) | 2000-04-26 | 2000-04-26 | Anti-oxidation process for natural bee glue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00114519A CN1274535A (en) | 2000-04-26 | 2000-04-26 | Anti-oxidation process for natural bee glue |
Publications (1)
| Publication Number | Publication Date |
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| CN1274535A true CN1274535A (en) | 2000-11-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00114519A Pending CN1274535A (en) | 2000-04-26 | 2000-04-26 | Anti-oxidation process for natural bee glue |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102429140A (en) * | 2011-10-27 | 2012-05-02 | 喀左县春蕾特产品开发中心 | Supercritical CO2 fluid extraction method of propolis |
| US8955993B2 (en) | 2011-10-27 | 2015-02-17 | Kabushiki Kaisha Tokai-Rika-Denki-Seisakusho | Mirror device for vehicle |
| CN104886590A (en) * | 2015-04-25 | 2015-09-09 | 安徽蜂献蜂业有限公司 | Natural propolis antioxidant and preparation method thereof |
| CN114028429A (en) * | 2021-11-22 | 2022-02-11 | 江苏鸿祺生物科技有限公司 | Processing technology of propolis active component supercritical extraction raw material |
-
2000
- 2000-04-26 CN CN00114519A patent/CN1274535A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102429140A (en) * | 2011-10-27 | 2012-05-02 | 喀左县春蕾特产品开发中心 | Supercritical CO2 fluid extraction method of propolis |
| CN102429140B (en) * | 2011-10-27 | 2013-07-24 | 喀左县春蕾特产品开发中心 | Supercritical CO2 fluid extraction method of propolis |
| US8955993B2 (en) | 2011-10-27 | 2015-02-17 | Kabushiki Kaisha Tokai-Rika-Denki-Seisakusho | Mirror device for vehicle |
| CN104886590A (en) * | 2015-04-25 | 2015-09-09 | 安徽蜂献蜂业有限公司 | Natural propolis antioxidant and preparation method thereof |
| CN114028429A (en) * | 2021-11-22 | 2022-02-11 | 江苏鸿祺生物科技有限公司 | Processing technology of propolis active component supercritical extraction raw material |
| CN114028429B (en) * | 2021-11-22 | 2022-12-16 | 江苏鸿祺生物科技有限公司 | Processing technology of propolis active component supercritical extraction raw material |
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