CN1271202C - Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour - Google Patents
Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour Download PDFInfo
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- CN1271202C CN1271202C CN 200410020832 CN200410020832A CN1271202C CN 1271202 C CN1271202 C CN 1271202C CN 200410020832 CN200410020832 CN 200410020832 CN 200410020832 A CN200410020832 A CN 200410020832A CN 1271202 C CN1271202 C CN 1271202C
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Abstract
The present invention relates to a Sphingomonas bacterial strain and the application of the bacterial strain in the decolorization of anthraquinone dye wastewater, which belongs to the technical field of biological engineering and environmental engineering. The Sphingomonas bacterial strain of the present invention is Sphingomonas xenophaga QYY which is preserved in the Common Micro-organisms Center of CCCCM from Jun. 1st, 2004, and the preservation number of the bacterial strain is CGMCC No. 1172. The bacterial strain is obtained by separating the sludge drained from the sewage draining exit of a bromaminic acid manufacturing shop of a chemical plant in Zhaoyuan of Shandong Province; the bacterial strain can take bromaminic acid which is the intermediate of the anthraquinone dye as a unique carbon source, a nitrogen source and an energy source for growth, the cells of the bacterial strain are treated by liquid culture, both rest cells and immobilized cells can destroy anthraquinone loops, and the cultured liquid of the bacterial strain can serve as a biologically strengthening preparation to decolorize the anthraquinone dye wastewater. The bacterium has the advantages of strong decolorizing power and high speed of degradation, and the bacterium is suitable for treating industrial water.
Description
Technical field
The invention belongs to biotechnology, field of environment engineering technology.Relate to Sphingol single-cell bacterial strain and application in the anthraquinone dye wastewater decolouring thereof.
Background technology
Because the DYE PRODUCTION process efficiency has lowly caused a large amount of unemployed dyestuffs directly to enter water body.The color that waste water from dyestuff presents has shown that directly water body is polluted.Therefore, dyestuff in the water body being removed is the focus of environmental area research.Compare with azoic dyestuff, anthraquinone dye is restricted owing to its Stability Analysis of Structures makes its biological degradation.Therefore, up to the present, still very limited about the biodegradable work of anthraquinone dye.Itoh ketal etc. have studied the biological degradation situation of Bacillus Subtilis to anthraquinone dye C.I.Risperse Red15.Jiang and Bishop have reported with the rotation membrane bioreactor and have carried out the acid anthraquinone dye of aerobic degradation.G.M Walkeret has studied the biological degradability and the biological adsorption of acid anthraquinone dye.
As a kind of main anthraquinone dye intermediate, bromamine acid (ABAS) derivative is used for dyestuff and textile industry by frequent.But, make progress less both at home and abroad about the work of ABAS biological degradation aspect.For this reason, filter out the bacterial strain that bromamine acid is had the efficient degradation ability from nature, thereby be made into biological bacteria preparation, the improvement that is applied to this class waste water from dyestuff will have actual using value.
Usually, with the dyestuff degradation bacteria strains great majority of report all by metabolism altogether or can be that sole carbon source grows degradation of dye with the target substrates, the function of these strains for degrading dyestuffs has been subjected to various restrictions to a certain extent, therefore, remain to be developed about the biodegradable Microbial resources of these dyestuffs.From the chemical structure angle, anthraquinone dyes belongs to the condensed ring aromatic compound.To the modal microorganism of aromatic compound biodegrade is Pseudomonas.In recent years, utilize 16S rRNA that bacterial classification is identified, defined a class again aromatic compound is had the Pseudomonas of general degradation capability, promptly Sphingol single-cell belongs to (Sphingomonas), and this Pseudomonas belongs to modification bacterial alpha subclass.Because traditional division bacteria method has limitation, so all the time, this Pseudomonas position of on systematics, independently not evolving.Studies show that Sphingol single-cell has the wide spectrum degrading activity to heteroplasia type compound, has important ecological significance.Therefore, the new bacterial strain of this Pseudomonas that any strain filters out from nature, it all is essential that its Physiology and biochemistry and function thereof are carried out deep research, will increase new member for the biodegradable Microbial resources of heteroplasia type compound.
The objective of the invention is to provides bacteria preparation efficiently for the biological treatment of anthraquinone dye wastewater, strengthening the decolouring of anthraquinone dye wastewater handles, and isolate a strain Sphingol single-cell from occurring in nature, it can be unique carbon, nitrogen and energy growth with bromamine acid, and anthraquinone dye wastewater is had decolorization efficiently.
Summary of the invention
Purpose of the present invention is exactly that biological treatment for anthraquinone dye wastewater provides bacteria preparation efficiently, strengthening the decolouring of anthraquinone dye wastewater handles, isolate a strain Sphingol single-cell from occurring in nature, can be unique carbon, nitrogen and energy growth with bromamine acid, having efficiently to anthraquinone dye wastewater, the Sphingol single-cell of decolorization belongs to bacterial strain and the application in the anthraquinone dye wastewater decolouring thereof.
Technical solution of the present invention is, it is Sphingomonas xenophaga QYY that Sphingol single-cell provided by the invention belongs to bacterial strain, be preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC NO.1172 on June 1st, 2004.Address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, 100080.
It is to separate to obtain from the mud of chemical plant, Zhaoyuan, Shandong bromamine acid production plant sewage draining exit that this Sphingol single-cell belongs to bacterial strain.
Its concrete screening step is as follows:
Gather the aerobic activated sludge sample from Zhaoyuan, Shandong chemical general factory bromamine acid production plant sewage draining exit, tame enrichment culture as the bacterium source.Be about to the mud of getting and make suspension liquid, get supernatant liquor and be added in the activation enrichment medium (substratum composition g/l: extractum carnis 5, peptone 10, NaCl 5) enrichment, wherein substratum is at 121 ℃, and autoclaving used after 20 minutes.And then to forward to the bromamine acid be that (substratum is formed (g/l): (NH in the substratum of sole carbon source
4)
2SO
42, KH
2PO
41.3, Na
2HPO
42, micro-1ml/l) domestication, wherein trace element is formed (g/l): (MnSO
4H
2O 39.9; ZnSO
49H
2O68.55; (NH
4)
6Mo
7O
244H
2O34.7).The disposable acclimation method that adds high concentrations of compounds is adopted in domestication, when each acclimation period finishes, culture is got certain volume to join in the fresh sole carbon source substratum, and improve constantly the concentration of bromamine acid, simultaneously, constantly reduce the amount of nitrogenous source, until mixed bacteria liquid can be unique carbon, nitrogen and energy growth with bromamine acid, this mixed bacteria liquid is coated on the solid-based basal culture medium that contains the finite concentration bromamine acid, carry out the flat board coating repeatedly, isolate a strain bacterial strain Sphingol single-cell that bromamine acid has the efficient decolorizing ability is belonged to bacterial strain;
This Sphingol single-cell belongs to bacterial strain and suits to grow in the developing medium of neutrality and slant acidity (PH 6.5～7.0), suitable growth temperature is 25～35 ℃, its bacteriology morphological specificity: be gram negative bacillus, be of a size of 0.3～0.4 μ m * 1.0～1.4 μ m, atrichia, cell is yellow, aerobic, the catalase positive, oxidase positive, no denitrification can be sole carbon, nitrogenous source growth with the bromamine acid.
Sphingol single-cell belongs to bacterial strain and destroys the anthraquinone ring structure, makes bromamine acid disappear at the maximum absorption band 485nm of visible region, thereby makes the decoloring dye waste water that contains bromamine acid.
The rest cell that Sphingol single-cell belongs to bacterial strain destroys the anthraquinone ring structure, makes bromamine acid disappear at the maximum absorption band 485nm of visible region, thereby makes the decoloring dye waste water that contains bromamine acid.
The immobilized cell that Sphingol single-cell belongs to bacterial strain destroys the anthraquinone ring structure, makes bromamine acid disappear at the maximum absorption band 485nm of visible region, thereby makes the decoloring dye waste water that contains bromamine acid.
The cell liquid culture that Sphingol single-cell belongs to bacterial strain destroys the anthraquinone ring structure, makes bromamine acid disappear at the maximum absorption band 485nm of visible region, thereby makes the decoloring dye waste water that contains bromamine acid.
This Sphingol single-cell belongs to the strain culturing feature:
This bacterium on solid LB substratum 30 ℃ cultivated 2-3 days, bacterium colony is little, and is rounded, neat in edge, the surface is dry and astringent, thalline presents dark yellow, very easily provokes.
This Sphingol single-cell belongs to the bacterial strain physiological and biochemical property, sees Table 1:
Table 1 Sphingol single-cell belongs to the bacterial strain physiological and biochemical property
| Test subject | The result | Test subject | The result | Test subject | The result | Test subject | The result |
| The cell color catalase oxidizing ferment that concerns of Gram’s staining cell shape flagellum and oxygen produces fluorchrome generation levulan generation pyocyanin hydrolysis aesculin gelatin hydrolysate | Negative shaft-like without aerobic yellow++---+- | Nitrate reduction utilizes citrate to utilize 4 ℃ of growths of 41 ℃ of growths of malonate arginine dihydrolase denitrification accumulation PHB O/F test glucose to produce acid | --------oxidation+ | Utilize sole carbon source growth dextrose fructose trehalose Valine Beta-alanine DL-arginine acetate sweet mellow wine wood sugar arabinose rhamnose mannose | + - - - - - - - - + - - | Galactolipin sucrose propionate sorbierite maltose D-tartrate leucine lactose L-Histidine L-Trp alpha-ketoglutarate phenylacetate | + - - - + - + - - - - - |
According to features such as this strain morphology and Physiology and biochemistries, can identify that it is the bacterial strain that Sphingol single-cell belongs to.By the 16S rDNA of this bacterial strain that increases, having obtained length is the 16S rDNA complete sequence of 1481bp.The PCR primer adopts universal primer F8 (5 '-AGA GTT TGA TCA TGG CTC AG-3 ') and the R1522 (5 '-AAG GAC GTC ATC CAG CCG CA-3 ') of bacterial 16 S rDNA.(GeneAmp, PCR System 9700) carries out amplified reaction with the PCR instrument.The reaction system cumulative volume is 25 μ l: ultrapure water 16.2 μ l, and 10xBuffer 2.5 μ l, dNTP 2 μ l, F8 primer 1 μ l, R22 primer 1 μ l, ExTaq enzyme 0.3 μ l, total DNA do to get 2 μ l in reaction system after certain dilution.94 ℃ of pre-sex change 5min, through 30 circulations, wherein, and 98 ℃ of sex change 20s, 55 ℃ of annealing 40s, 72 ℃ are extended 1.5min, extend 7min at 72 ℃ again after 30 circulations, 1% agarose gel electrophoresis, EB dyeing back ultraviolet detection.Carry out homology relatively by the Blast program, the homology that shows this bacterial strain and Sphingomonas xenophaga is up to 99%.
Sphingol single-cell of the present invention belongs to bacterial strain both can grow in eutrophy substratum LB, also can grow in the basic medium that with the bromamine acid is sole carbon, nitrogenous source.Need before using to tame under the condition that bromamine acid exists, bacterial strain is at 30 ℃, and pH 6.5, carry out aerobic cultivation.
It is extremely strong to the decoloring ability of bromamine acid that this Sphingol single-cell belongs to bacterial strain, and after this bacterial strain effect, the ultraviolet-visible of bromamine acid scanning spectrogram does not have absorption in the visible region, and nutrient solution is near colourless.
Sphingol single-cell of the present invention belongs to bacterial strain, can with fresh culture vegetative period bacterial classification or the rest cell of making and immobilized cell thereof to the bromamine acid processing of decolouring.
The beneficial effect that the present invention reached is: Sphingol single-cell provided by the invention belongs to bacterial strain has extremely strong decoloring ability to bromamine acid, and degradation speed is fast, and to the bromamine acid less than 800mg/l, at short notice, percent of decolourization is up to more than 90%.And, through this invention bacterial strain effect contain the bromamine acid waste water from dyestuff, its effluent color dilution reduces greatly, near colourless.This bacterial strain can be used as biological bacteria preparation, is added in the existing dye waste water treatment system, strengthens the processing power of former processing system.In addition, because the singularity of this bacterial classification exocytosis thing makes it that wide application potential be arranged in Treatment of Industrial Water.
Description of drawings
Fig. 1 be Sphingol single-cell of the present invention belong to bacterial strain in enrichment medium, grow with bromamine acid degradation curve figure.
Fig. 2 is the degraded situation map of rest cell of the present invention to the different concns bromamine acid.
Fig. 3 is that Sphingol single-cell of the present invention belongs to bacterial strain degraded situation map to the different concns bromamine acid in sole carbon, nitrogenous source substratum.
Fig. 4 is that different bag bacterium amount immobilized spherules of the present invention processing in simulation SBR system contains ABAS waste water situation map.
Among the figure :-●-be degradation curve ,-zero-be growth curve ,-be theoretical value, ■ is an experimental value ,-◆-be water inlet bromamine acid concentration ,--be 2% bag bacterium amount ,-▲-be that 5% bag bacterium is measured ,-*-be 10% bag bacterium amount.
Embodiment
The invention will be further described below in conjunction with accompanying drawing.
Embodiment 1
Sphingol single-cell provided by the invention belongs to the screening step of bacterial strain:
Gather the aerobic activated sludge sample from Zhaoyuan, Shandong chemical general factory bromamine acid production plant sewage draining exit, tame enrichment culture as the bacterium source.Be about to the mud of getting and make suspension liquid, get supernatant liquor and be added to enrichment in the activation enrichment medium, substratum is formed (g/l): extractum carnis 5, peptone 10, NaCl 5, and wherein substratum is at 121 ℃, and autoclaving used after 20 minutes.And then to forward to the bromamine acid be to tame in the substratum of sole carbon source, and substratum is formed (g/l): (NH
4)
2SO
42, KH
2PO
41.3, Na
2HPO
42, micro-1ml/l, wherein trace element is formed (g/l): (MnSO
4H
2O 39.9, ZnSO
49H
2O 68.55, (NH
4)
6Mo
7O
244H
2O 34.7.The disposable acclimation method that adds high concentrations of compounds is adopted in domestication, when each acclimation period finishes, culture is got certain volume to join in the fresh sole carbon source substratum, and improve constantly the concentration of bromamine acid, simultaneously, constantly reduce the amount of nitrogenous source, until mixed bacteria liquid can be unique carbon, nitrogen and energy growth with bromamine acid, this mixed bacteria liquid is coated on the solid-based basal culture medium that contains the finite concentration bromamine acid, carry out the flat board coating repeatedly, isolate a strain has the efficient decolorizing ability to bromamine acid bacterial strain QYY; And this bacterial strain is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC NO.1172 on June 1st, 2004;
This Sphingol single-cell belongs to the bacterial strain bacterial strain and suits to grow in the developing medium of neutrality and slant acidity (PH 6.5), be fit to growth temperature at 30 ℃, its bacteriology morphological specificity: be gram negative bacillus, be of a size of 0.3-0.4 μ m * 1.0-1.4 μ m, atrichia, cell is yellow, aerobic, the catalase positive, oxidase positive, no denitrification can be sole carbon, nitrogenous source growth with the bromamine acid.
Sphingol single-cell provided by the invention belongs to the 16S rRNA amplification of bacterial strain:
By the 16S rDNA of this bacterial strain that increases, having obtained length is the 16S rDNA complete sequence of 1481bp.The PCR primer adopts universal primer F8 (5 '-AGA GTT TGA TCA TGG CTCAG-3 ') and the R1522 (5 '-AAG GAC GTC ATC CAG CCG CA-3 ') of bacterial 16 S rDNA.(GeneAmp, PCR System 9700) carries out amplified reaction with the PCR instrument.The reaction system cumulative volume is 25 μ l: ultrapure water 16.2 μ l, and 10xBuffer 2.5 μ l, dNTP 2 μ l, F8 primer 1 μ l, R22 primer 1 μ l, ExTaq enzyme 0.3 μ l, total DNA do to get 2 μ l in reaction system after certain dilution.94 ℃ of pre-sex change 5min, through 30 circulations, wherein, and 98 ℃ of sex change 20s, 55 ℃ of annealing 40s, 72 ℃ are extended 1.5min, extend 7min at 72 ℃ again after 30 circulations, 1% agarose gel electrophoresis, EB dyeing back ultraviolet detection.Carry out homology relatively by the Blast program, the homology that shows this bacterial strain and Sphingomonas xenophaga is up to 99%, and sequence as shown in Figure 5.
The preparation Sphingol single-cell belongs to the cell liquid culture of bacterial strain bacterial strain:
Sphingol single-cell on the picking LB solid medium belongs to bacterial strain bacterial strain list bacterium colony in the LB liquid nutrient medium that sterilization is housed, wherein the concentration of bromamine acid is 100mg/l, in 30 ℃, pH 6.5,150r/min, carry out aerobic cultivation 12-24 hour, getting cultured bacterium liquid 1ml is seeded in and is equipped with in the 250ml Erlenmeyer flask of basic medium that 100ml contains sterilization bromamine acid (100mg/l), 30 ℃, pH 6.5,150r/min carries out aerobic cultivation 48 hours, is the cell liquid culture that Sphingol single-cell belongs to bacterial strain.
The preparation Sphingol single-cell belongs to the rest cell of bacterial strain:
Sphingol single-cell is belonged to bacterial strain in 8000r/min, and 4 ℃ of following centrifugal 10min are with phosphoric acid buffer washing 3 times, should precipitate with same damping fluid is resuspended, make that rest cell concentration was 10mg/l, frozen in 4 ℃, standby, promptly make the rest cell that Sphingol single-cell belongs to bacterial strain.
The preparation Sphingol single-cell belongs to the immobilized cell of bacterial strain:
[1] heating of 1.1g sodium alginate is dissolved in the 20ml deionized water, and is cooled to 30 ℃;
[2] place 30 ℃ of waters bath with thermostatic control to temperature-stable the cell suspension 20ml among the embodiment 4;
[3] 1 sodium alginate aqueous solution in [1] is mixed with [2] medium volume bacteria suspension and stir;
[4] mixture in [3] is splashed into 5% CaCl with injector for medical purpose
2In the solution, make bead, and in 4 ℃ of refrigerators crosslinked 4h.
[5] with physiological saline bead is washed twice, be stored in the physiological saline, and preserve standby in 4 ℃ of refrigerators.
Embodiment 2
To the application of bromamine acid decolouring research, its step is as follows in the eutrophy substratum in the present invention:
[1] add 50ml LB substratum in the Erlenmeyer flask of 250ml, adding bromamine acid again, to make its final concentration be 100mg/l, and it is standby to sterilize.
[2] to belong to the cell liquid culture of bacterial strain or embodiment be that the rest cell that the Sphingol single-cell of preparation belongs to bacterial strain adds in the Erlenmeyer flask of above-mentioned [1] to the Sphingol single-cell that embodiment 1 is made, 30 ℃, and 150r/min, aerobic cultivation.Every 12 hours sampling and measuring.Fig. 1 grows in enrichment medium and bromamine acid degraded situation for Sphingol single-cell belongs to bacterial strain; As seen from the figure, the degraded of bromamine acid occurs in the early stage rather than logarithmic phase of strain growth; And as can be seen, a spot of growth of thalline is enough to make the bromamine acid degraded, inoculates after 14 hours, and the percent of decolourization of bromamine acid is up to 100%.Fig. 2 is the influences of different bromamine acid starting point concentrations to rest cell degraded bromamine acid, and as shown in Figure 2: rest cell meets typical substrate inhibition model to the degraded of bromamine acid.
Embodiment 3
The present invention with the bromamine acid is being the application that decolouring is studied to bromamine acid in sole carbon, the nitrogenous source substratum:
It is the basic medium of sole carbon, nitrogenous source that LB substratum among the embodiment 1 is changed to the bromamine acid, and other step is with embodiment 2.Fig. 3 is the degraded situation of grown cell to the different concns bromamine acid; As seen from Figure 3, it is extremely strong to the decoloring ability of bromamine acid that Sphingol single-cell belongs to bacterial strain, can tolerate bromamine acid concentration up to 1000ppm.
Embodiment 4
To the application of bromamine acid waste water decoloring, its step is as follows in laboratory simulation sbr reactor device in the present invention:
[1] at first, operation SBR treatment system is the laboratory simulation reactor.The 50ml mud suspension of in the 250ml Erlenmeyer flask, packing into, sludge concentration is 3g/l,, 30 ℃, 150r/min, aerobic cultivation.Comprise five stages (one-period is 12h) reaction time: water inlet 0.5h; Aeration 10h; Precipitation 1h; Draining and idle 0.5h.
[2] bromamine acid with sterilization adds in the above-mentioned Erlenmeyer flask, and its concentration is determined according to test requirements document.
[3] immobilized cell that the Sphingol single-cell of example 1 preparation is belonged to bacterial strain drops in the Erlenmeyer flask of above-mentioned [1], 30 ℃, 150r/min, aerobic cultivation, every 12 hours sampling and measuring, Fig. 5 handled in simulation SBR system for different bag bacterium amount immobilized spherules and contains ABAS waste water situation, from curvilinear trend as can be seen, the immobilized spherule of different bag bacterium amounts is to the decolorizing effect difference of bromamine acid, and the treatment effect of 10% bag bacterium amount is best.
＜110〉Dalian University of Technology's environment and life institute
＜120〉Sphingol single-cell belongs to bacterial strain and the application in the anthraquinone dye wastewater decolouring thereof
＜160〉the sequence sum 1
<211>1481bp
<212>DNA
＜213〉Sphingol single-cell (Sphingomonas xenophaga)
<220>
<221>gene
＜120〉Sphingol single-cell belongs to the 16S rRNA complete sequence of bacterial strain
1 aaggaggtga tccagccgca ggttccccta cggctacctt gttacgactt caccccagtc
61 tctaaaccca ccgtggtcac ctgtctccct tgcgggttaa cgcagtgcct tcgggtgaat
121 ccaaatccca tggtgtgacg ggcggtgtgt acaaggcctg ggaacgtatt caccgcggca
181 tgctgatccg cgattactag cgattccgcc ttcatgctct cgagttgcag agaacaatcc
241 gaactgagac gacttttgga gattagctac cgctcgcacg gttgcagccc actgtagtcg
301 ccattgtagc acgtgtgtag cccagcgcgt aagggccatg aggacttgac gtcatcccca
361 ccttcctccg gcttatcacc ggcggttcct ttagagtacc caaccaaatg ctggcaacta
421 aaggcgaggg ttgcgctcgt tgcgggactt aacccaacat ctcacgacac gagctgacga
481 cagccatgca gcacctgtca ctcatccagc cgaactgaag gaaatcatct ctgaaatccg
541 cgatgaggat gtcaaacgct ggtaaggttc tgcgcgttgc ttcgaattaa accacatgct
601 ccaccgcttg tgcaggcccc cgtcaattcc tttgagtttt aatcttgcga ccgtactccc
661 caggcggata acttaatgcg ttagctgcgc caccgaaaca ccatgtgccc cggcagctag
721 ttatcatcgt ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt
781 cgcacctcag cgtcaatact tgtccagcga gccgccttcg ccactggtgt tcttccgaat
841 atctacgaat ttcacctcta cactcggaat tccactcgcc tctccaagat tctagcaatc
901 cagtctcaaa ggcagttccg gggttgagcc ccgggctttc acctctgact taaatcgccg
961 cctacgtgcg ctttacgccc agtaattccg aacaacgcta gctccctccg tattaccgcg
1021 gctgctggca cggagttagc cggagcttat tctcccgata ctgtcattat catctcgggt
1081 aaaagagctt tacaacccta aggccttcat cactcacgcg gcattgctgg atcaggcttt
1141 cgcccattgt ccaatattcc ctactgctgc ctcccgtagg agtctgggcc gtgtctcagt
1201 cccagtgtgg ctgatcatcc tctcagacca gctaaggatc gttgccttgg tgagccttta
1261 ccccaccaac tagctaatcc tacgcgggct catccctggg cgataaatct ttggactttc
1321 gtcatcatcc ggtattagct tcagtttccc gaagttattc cgaacccaag ggcagattcc
1381 cacgcgttac gcacccgtgc gccactagac ccgaaggtct cgttcgactt gcatgtatta
1441 ggcatgccgc cagcgttcgt tctgagccat gatcaaactc t
Claims (6)
1. Sphingol single-cell belongs to bacterial strain, it is characterized in that, it was Sphingomonasxenophaga QYY that Sphingol single-cell belongs to bacterial strain, and this bacterial strain is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC No.1172 on June 1st, 2004.
2. Sphingol single-cell according to claim 1 belongs to bacterial strain, it is characterized in that, separates obtaining from the mud of chemical plant, Zhaoyuan, Shandong bromamine acid production plant sewage draining exit; This bacterial strain is cultivated in pH value 6.5～7.0 substratum media, and the growth temperature that is fit to is 25～35 ℃; Its bacteriology morphological specificity: be gram negative bacillus, be of a size of 0.3～0.4 μ m * 1.0～1.4 μ m, atrichia, cell are yellow, aerobic, the catalase positive, and oxidase positive, no denitrification can be sole carbon, nitrogenous source growth with the bromamine acid.
3. the described Sphingol single-cell of claim 1 belongs to the application of bacterial strain in the anthraquinone dye wastewater decolouring, it is characterized in that, Sphingol single-cell belongs to bacterial strain and destroys the anthraquinone ring structure, makes bromamine acid disappear at the maximum absorption band 485nm of visible region, thereby makes the decoloring dye waste water that contains bromamine acid.
4. Sphingol single-cell according to claim 3 belongs to the application of bacterial strain in the anthraquinone dye wastewater decolouring, it is characterized in that, the rest cell that Sphingol single-cell belongs to bacterial strain destroys the anthraquinone ring structure, make bromamine acid disappear, thereby make the decoloring dye waste water that contains bromamine acid at the maximum absorption band 485nm of visible region.
5. Sphingol single-cell according to claim 3 belongs to the application of bacterial strain in the anthraquinone dye wastewater decolouring, it is characterized in that, the immobilized cell that Sphingol single-cell belongs to bacterial strain destroys the anthraquinone ring structure, make bromamine acid disappear, thereby make the decoloring dye waste water that contains bromamine acid at the maximum absorption band 485nm of visible region.
6. Sphingol single-cell according to claim 3 belongs to the application of bacterial strain in the anthraquinone dye wastewater decolouring, it is characterized in that, the cell liquid culture that Sphingol single-cell belongs to bacterial strain destroys the anthraquinone ring structure, make bromamine acid disappear, thereby make the decoloring dye waste water that contains bromamine acid at the maximum absorption band 485nm of visible region.
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| CN101973640B (en) * | 2010-09-21 | 2011-11-16 | 东北电力大学 | Method for treating malachite green dye waste water |
| CN101974471A (en) * | 2010-11-12 | 2011-02-16 | 东华大学 | Sphingosine monad DX-T3-03 strain and extracting method thereof |
| CN103466807A (en) * | 2013-09-09 | 2013-12-25 | 江苏大学 | Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora |
| CN103466805A (en) * | 2013-09-09 | 2013-12-25 | 江苏大学 | Technique for removing anthraquinone compounds in wastewater by using Ceriporiopsis subvermispora thallus |
| CN103466804B (en) * | 2013-09-09 | 2016-04-06 | 江苏大学 | Aspergillus oryzae cell is utilized to remove anthraquinone analog compound Technology in waste water |
| CN103979705B (en) * | 2014-04-18 | 2015-05-20 | 绍兴奇彩化工有限公司 | Method for recovering potassium nitrate from anthraquinone dye alkali wastewater |
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