CN103466804B - Aspergillus oryzae cell is utilized to remove anthraquinone analog compound Technology in waste water - Google Patents
Aspergillus oryzae cell is utilized to remove anthraquinone analog compound Technology in waste water Download PDFInfo
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Abstract
The invention discloses and utilize aspergillus oryzae cell to remove anthraquinone analog compound Technology in waste water, relate to bioengineering field.Namely utilize aspergillus oryzae as starting strain, through liquid submerged culture, liquid thalline enlarged culturing, obtain aspergillus oryzae cell, utilize this thalline to remove the technique of anthraquinone analog compound in waste water.This Technology anthraquinone analog compound clearance is adopted to reach 70 ~ 100%.
Description
Technical field
The present invention relates to technical field of bioengineering, refer in particular to and utilize aspergillus oryzae cell to remove containing the anthraquinone analog compound in the waste water of anthraquinone analog compound.Concentrated by raw wastewater or be diluted to Anthraquinone after 0.5 ~ 10 grams per liter, dropping into aspergillus oryzae cell, anthraquinone analog compound is removed in aeration-agitation reaction.The clearance applying this technique anthraquinone analog compound can reach 60 ~ 100%.
Background technology
Anthraquinone compounds is of a great variety, is important industrial chemicals and intermediate, bright-colored, scope is wide, thus widespread use in the industries such as weaving, printing and dyeing, not only can be used for the printing and dyeing of terylene and BLENDED FABRIC, also can be used for the printing and dyeing of polyamide fibre, acrylic fibers, polypropylene fibre, vinegar fibre, wool, silk.Simultaneously along with the lifting day by day of environmental requirement, azoic dyestuff forbidding range extension, also makes the importance of anthraquinone dye more outstanding, makes its application in printing and dyeing industry more and more extensive.But the anthraquinone analog compound not only factory effluent water yield is large, and this compound colourity is high, and solubleness is low! Fusing point is high! A stable in properties! Be difficult to biological degradation, have again higher lipotropy, in the sewage of directly discharge, anthraquinone passes through sedimentation! Food chain enrichment etc. effect can prolonged stay in water body! Soil! In settling and air.Therefore explore the technology of effectively degraded anthraquinone analog compound, the harmony for society is of great significance with development tool.
Have several about the process patent containing anthraquinone analog compound waste water at present, but main method is the recycling of anthraquinone, microbiological deterioration and oxidation removal.As patent " 1; improvement of 4-dihydroxyanthraquinone factory effluent and its recovery method as resource " (application number 00112386.6) discloses and utilizes macroporous resin adsorption, the method of the anthraquinone analog compound recycled in Isosorbide-5-Nitrae-dihydroxyanthraquinone factory effluent is resolved again with sodium hydroxide; Patent " recovery method of a kind of Isosorbide-5-Nitrae-dihydroxyanthraquinone processing wastewater " (application number 200410013484.X) discloses the method utilizing the technology recycling anthraquinones such as concentrated, cooling, crystallization, separation and drying.Patent " Sphingomonas strain and the application in anthraquinone dye wastewater decolouring thereof " (application number 200410020832.6) discloses Sphingomonas, application in anthraquinone dye wastewater decolouring, this bacterium decoloring ability is strong, degradation speed is fast, but can only be suitable for lower concentration anthraquinone waste water; Adopt electrochemical oxidation (patent No. 200910029887.6) and photochemical catalytic oxidation (200710011629.6) in addition, these two kinds of methods also need further process.Also many reports are had with these method correlative theses.Other doctor's or master's degree paper reports Phanerochaete chrysosporium absorption degradation anthraquinone analog compound mechanism and is studied, but does not relate to its Technology.
The present inventor through deep research, find out one utilize aspergillus oryzae (
aspergillusoryzae) thalline, remove anthraquinone analog compound technique in waste water in ventilation and stirring reaction for some time, this process time is short, and clearance is high.
Summary of the invention
The present invention is to provide aspergillus oryzae (
aspergillusoryzae) biotechnology of anthraquinone analog compound in degrading waste water.Waste water is after aspergillus oryzae degraded, and its anthraquinone analog compound clearance can arrive 60 ~ 100%.
A kind of aspergillus oryzae degraded of the present invention anthraquinone analog compound Technology, carry out according to following step: take aspergillus oryzae as starting strain, carry out test tube enlarged culturing, liquid submerged culture, thalline fluid enlargement culture, collected by centrifugation thalline, then remove anthraquinone analog compound in waste water with thalline.
The present invention's bacterial classification used is aspergillus oryzae any one bacterial classification, as the bacterial classification of China General Microbiological culture presevation administrative center (CCGMC), Chinese agriculture Microbiological Culture Collection administrative center (ACCC), Chinese industrial Microbiological Culture Collection administrative center (CICC), Chinese medicine Microbiological Culture Collection administrative center (CMCC), National Veterinary Culture Collection (CVCC) and microbiotic bacterial classification preservation pipe reason center etc. preservation.
The present invention the waste water that is suitable for be that any one contains the waste water of anthraquinone analog compound, as the waste liquid of fermentative Production anthraquinone analog compound, extract from plant anthraquinone analog compound waste liquid or take anthraquinone analog compound as the waste liquid of other products of Material synthesis.
Test tube enlarged culturing base in wherein said test tube enlarged culturing is potato sucrose substratum (" the industrial microorganism experimental technique handbook " of Zhu Gejian, Wang Zhengxiang chief editor, 1994,367 pages.
Liquid submerged culture base in wherein said liquid submerged culture and in thalline fluid enlargement culture and liquid thalline substratum are: glucose 5 ~ 30 grams per liter, peptone 0 ~ 5 grams per liter, yeast extract paste 0 ~ 5 grams per liter, magnesium sulfate 0.2 ~ 1 grams per liter, tween-80 0.5 ~ 10 grams per liter, potassium primary phosphate 2 ~ 9 grams per liter, pH5 ~ 8,120 ~ 140 DEG C of sterilizings 20 ~ 40 minutes; Wherein said shake-flask culture processing condition are, inoculate a ring aspergillus oryzae test tube slant spore (1 × 10
6) in the 250mL triangular flask that 40 ~ 120mL substratum is housed, at rotating speed: 150 revs/min, 25 ~ 35 DEG C, cultivate 24 ~ 72 hours; Wherein said yeast culture technique is, by 1 ~ 20%(volume ratio) inoculum size shake-flask culture seed liquor is inoculated into liquid thalline enlarged culturing technique and is, temperature 25 ~ 35 DEG C, mixing speed 50 ~ 200 revs/min, air flow 0.2 ~ 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 18 ~ 72 hours; Nutrient solution centrifugal 5 ~ 20 minutes through 3000 ~ 6000rpm, obtains aspergillus oryzae cell.
The technique that thalline of the present invention removes anthraquinone analog compound in waste water is: above-mentioned aspergillus oryzae cell with contain 0.05 ~ 1 grams per liter anthraquinone analog compound waste water (by vacuum concentration or dilute regulate anthraquinone analog compound concentration) by 1:10 ~ 50(mass/volume) mix, temperature 25 ~ 60 DEG C, mixing speed 50 ~ 200 revs/min, air flow 0.2 ~ 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), react 3 ~ 15 hours.
After using this processing wastewater to degrade, anthraquinone analog compound clearance can reach 70-100%.
According to liquid thalline enlarged culturing technique of the present invention, in conjunction with the ABC of this area, can according to need of production, increase secondary, three grades of even level Four thalline tanks, expand the scale of production further, to carry out suitability for industrialized production.Secondary, three grades even level Four seeding tank adopt substratum identical with thalline fluid enlargement culture base with Shake flask medium, inoculum size is 5 ~ 20%, and culture condition is same as liquid thalline enlarged culturing condition.
Embodiment
According to the anthraquinone analog compound measuring method that this technique adopts be: accurate absorption 1,8-dihydroxyanthraquinone reference substance solution (0.522mg/ml) 1,3,5,8,10,15ml, put in 25ml volumetric flask respectively, fling to methyl alcohol, residue is with 0.5% magnesium acetate dissolve with ethanol solution and be diluted to scale, shake up, in 425nm place, make reference with 0.5% magnesium acetate ethanolic soln and measure optical density.With 1,8-dihydroxy-anthracene quinone content (y), (x) linear regression analysis is carried out to optical density, obtain equation of linear regression.Accurate pipette samples solution repeats aforesaid operations and measures optical density, utilizes equation of linear regression to calculate the content of general anthraquinone in each sample.
Anthraquinone analog compound clearance Re calculates by following formula (1):
(1)
In formula,
r t for the clearance of t time anthraquinone analog compound;
c 0 (mg/L) be the anthraquinone analog compound concentration of 0 hour;
c t (mg/L) be the concentration of absorption t hour anthraquinone analog compound.
In order to set forth the material and progress involved by technical scheme of the present invention further, give following examples, but these embodiments do not limit the scope of the invention in any form.
Embodiment 1
1. the making of test tube slant bacterial classification
At potato sucrose substratum (" the industrial microorganism experimental technique handbook " of Zhu Gejian, Wang Zhengxiang chief editor of new configuration, 1994,367 pages) middle access one ring aspergillus oryzae bacterial classification (China General Microbiological culture presevation administrative center CCGMC3.5232), 26 DEG C, incubation time 50 hours; 4 DEG C save backup.
2. the making of liquid submerged culture bacterial classification
Accurately take glucose 5 grams, peptone 0 gram, yeast extract paste 5 grams, 0.2 gram, magnesium sulfate, tween-80 0.5 gram, potassium primary phosphate 2 grams, water 1000mL, pH5, packing 250mL triangular flask, 50 grams every bottle, totally 20 bottles, 120 DEG C of sterilizings 40 minutes; After cooling, every bottle graft enters the aspergillus oryzae bacterial classification (1 × 10 that 4 DEG C, a ring is preserved
6individual spore), at 25 DEG C, 150 revs/min, cultivate 72 hours;
3. thalline fluid enlargement culture
Accurately take glucose 50 grams, peptone 0 gram, yeast extract paste 50 grams, 2 grams, magnesium sulfate, tween-80 5 grams, potassium primary phosphate 20 grams, water 10L, is loaded in the seeding tank of 15L, 120 DEG C of sterilizings 20 minutes; After sterilizing cooling, temperature 25 DEG C, mixing speed 50 revs/min, air flow 0.2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 72 hours; Nutrient solution centrifugal 20 minutes through 3000rpm, obtains aspergillus oryzae cell.
4. remove anthraquinone analog compound in waste water
Take rheum officinale as raw material, decocting method is adopted to extract the waste liquid of anthraquinone analog compound through concentrated, anthraquinone analog compound concentration is 1 gram/L, thalline and waste liquid are by 1:50(mass/volume) mix, temperature 25 DEG C, mixing speed 60 revs/min, air flow 0.3:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), react after 3 hours, anthraquinone analog compound clearance reaches 71.3%.
Embodiment 2
1. the making of test tube slant bacterial classification
At potato sucrose substratum (" the industrial microorganism experimental technique handbook " of Zhu Gejian, Wang Zhengxiang chief editor of new configuration, 1994,367 pages) middle access one ring aspergillus oryzae bacterial classification (Chinese agriculture Microbiological Culture Collection administrative center ACCC30155), 30 DEG C, incubation time 100 hours; 7 DEG C save backup.
2. the making of liquid submerged culture bacterial classification
Accurately take glucose 150 grams, peptone 25 grams, yeast extract paste 25 grams, 0.4 gram, magnesium sulfate, tween-80 4 grams, potassium primary phosphate 5 grams, water 10L, pH6, packing 250mL triangular flask, 100 grams every bottle, totally 100 bottles, 130 DEG C of sterilizings 30 minutes; After cooling, every bottle graft enters the aspergillus oryzae bacterial classification (1 × 10 that 7 DEG C, a ring is preserved
6individual spore), at 30 DEG C, 150 revs/min, cultivate 50 hours;
3. thalline fluid enlargement culture
Accurately take glucose 2000 grams, peptone 250 grams, yeast extract paste 250 grams, 60 grams, magnesium sulfate, tween-80 60 grams, potassium primary phosphate 400 grams, water 100L, is loaded in the seeding tank of 130L, 130 DEG C of sterilizings 30 minutes; After cooling, temperature 30 DEG C, mixing speed 125 revs/min, air flow 1:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 50 hours; Nutrient solution centrifugal 13 minutes through 4500rpm, obtains aspergillus oryzae cell.
4. remove anthraquinone analog compound in waste water
Fusarium oxysporum fermentation is for the waste liquid of anthraquinone analog compound, the content of anthraquinone analog compound is made to reach the waste water 1000L of 0.2 gram/L through concentrated, thalline and waste liquid are by 1:30(mass/volume) mix, at temperature 45 C, mixing speed 120 revs/min, air flow 1:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), react after 7 hours, anthraquinone analog compound clearance reaches 87.5%.
Embodiment 3
1. the making of test tube slant bacterial classification
At potato sucrose substratum (" the industrial microorganism experimental technique handbook " of Zhu Gejian, Wang Zhengxiang chief editor of new configuration, 1994,367 pages) middle access one ring aspergillus oryzae bacterial classification (Chinese industrial Microbiological Culture Collection administrative center CICC2001), 35 DEG C, incubation time 144 hours; 10 DEG C save backup.
2. the making of liquid submerged culture bacterial classification
Accurately take glucose 300 grams, peptone 50 grams, yeast extract paste 0 gram, 10 grams, magnesium sulfate, tween-80 100 grams, potassium primary phosphate 90 grams, water 10L, pH7.5, packing 250mL triangular flask, every bottle of 120mL, totally 80 bottles, 140 DEG C of sterilizings 20 minutes; After cooling, every bottle graft enters the aspergillus oryzae bacterial classification (1 × 10 that 10 DEG C, a ring is preserved
6individual spore), at 35 DEG C, 150 revs/min, cultivate 72 hours;
3. one-level thalline fluid enlargement culture
Accurately take glucose 1500 grams, peptone 250 grams, yeast extract paste 0 gram, 50 grams, magnesium sulfate, tween-80 500 grams, potassium primary phosphate 450 grams, water 50L, is loaded in the first class seed pot of 70L, 140 DEG C of sterilizings 40 minutes; After sterilizing cooling, temperature 35 DEG C, mixing speed 200 revs/min, air flow 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 50 hours;
4. secondary thalline fluid enlargement culture
Accurately take glucose 15000 grams, peptone 2500 grams, yeast extract paste 0 gram, 500 grams, magnesium sulfate, tween-80 5000 grams, potassium primary phosphate 4500 grams, water 500L, be loaded in the secondary seed tank of 700L, 140 DEG C of sterilizings 40 minutes; After sterilizing cooling, temperature 35 DEG C, mixing speed 200 revs/min, air flow 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivate 18 hours; Nutrient solution centrifugal 5 minutes through 6000rpm, obtains aspergillus oryzae cell.
5. remove anthraquinone analog compound in waste water
Take anthraquinone analog compound as raw material, under the effect of catalyzer, the waste liquid of synthesizing nitryl anthraquinone, be diluted to the waste liquid 1000L that Anthraquinone is 0.05 gram/L, thalline and waste liquid are by 1:10(mass/volume) mix, at temperature 60 C, mixing speed 200 revs/min, air flow 2:1 volume/volume/minute (ventilation gas volume/fermentating liquid volume/minute), react after 15 hours, anthraquinone analog compound clearance reaches 100%.
Claims (1)
1. the method for an aspergillus oryzae degraded anthraquinone analog compound waste water, it is characterized in that carrying out according to following step: take aspergillus oryzae as starting strain, carry out test tube enlarged culturing, liquid submerged culture, thalline fluid enlargement culture, collected by centrifugation thalline, then remove anthraquinone analog compound in waste water with thalline;
whereinbacterial strain used is any strain bacterial classification of aspergillus oryzae;
The waste water that is suitable for be fermentative Production anthraquinone analog compound waste liquid, extract from plant anthraquinone analog compound waste liquid or take anthraquinone analog compound as the waste liquid of other products of Material synthesis;
Enlarged culturing base in wherein said test tube enlarged culturing is potato sucrose substratum;
Liquid submerged culture base in wherein said liquid submerged culture and thalline fluid enlargement culture and thalline liquid nutrient medium are: glucose 5 ~ 30 grams per liter, peptone 0 ~ 5 grams per liter, yeast extract paste 0 ~ 5 grams per liter, magnesium sulfate 0.2 ~ 1 grams per liter, tween-80 0.5 ~ 10 grams per liter, potassium primary phosphate 2 ~ 9 grams per liter, pH5 ~ 8,120 ~ 140 DEG C of sterilizings 20 ~ 40 minutes;
Wherein said liquid submerged culture processing condition are: the aspergillus oryzae test tube slant thalline of inoculation 3-5 block 5 × 5mm, in the 250mL triangular flask that 40 ~ 120mL liquid submerged culture base is housed, rotating speed 150 revs/min, 25 ~ 35 DEG C, is cultivated 24 ~ 72 hours;
Wherein said thalline fluid enlargement culture technique is: be by volume 1 ~ 20% inoculum size shake-flask culture seed liquor is inoculated in thalline fluid enlargement culture base, temperature 25 ~ 35 DEG C, mixing speed 50 ~ 200 revs/min, air flow, according to ventilation gas volume/fermentating liquid volume/minute be 0.2 ~ 2:1, is cultivated 18 ~ 72 hours; Nutrient solution centrifugal 5 ~ 20 minutes through 3000 ~ 6000rpm, obtains aspergillus oryzae cell;
Described by the technique of anthraquinone analog compound in thalline removal waste water is: above-mentioned aspergillus oryzae cell and the waste water containing 0.05 ~ 1 grams per liter anthraquinone analog compound are that 1:10 ~ 50 mix by mass/volume, wherein regulate anthraquinone analog compound concentration by vacuum concentration or dilution, temperature 25 ~ 60 DEG C, mixing speed 50 ~ 200 revs/min, air flow is according to ventilation gas volume/fermentating liquid volume/minute be 0.2 ~ 2:1, react 3 ~ 15 hours, can anthraquinone analog compound be removed.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5091089A (en) * | 1990-09-06 | 1992-02-25 | Development Center For Biotechnology | Microbial decolorization of wastewater |
| CN1618953A (en) * | 2004-06-24 | 2005-05-25 | 大连理工大学 | Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour |
| CN1792890A (en) * | 2005-11-16 | 2006-06-28 | 华东师范大学 | Process and apparatus for treating active dyeing waste water by white rot fungi biological membrane method |
| CN101734800A (en) * | 2008-11-07 | 2010-06-16 | 中国科学院沈阳应用生态研究所 | Method for decolorizing printing and dyeing waste water by adopting immobilized fungal mycelia |
| CN102674560A (en) * | 2012-05-29 | 2012-09-19 | 江苏大学 | Process for biodegrading yellow water of white spirit factory |
-
2013
- 2013-09-09 CN CN201310405488.1A patent/CN103466804B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5091089A (en) * | 1990-09-06 | 1992-02-25 | Development Center For Biotechnology | Microbial decolorization of wastewater |
| CN1618953A (en) * | 2004-06-24 | 2005-05-25 | 大连理工大学 | Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour |
| CN1792890A (en) * | 2005-11-16 | 2006-06-28 | 华东师范大学 | Process and apparatus for treating active dyeing waste water by white rot fungi biological membrane method |
| CN101734800A (en) * | 2008-11-07 | 2010-06-16 | 中国科学院沈阳应用生态研究所 | Method for decolorizing printing and dyeing waste water by adopting immobilized fungal mycelia |
| CN102674560A (en) * | 2012-05-29 | 2012-09-19 | 江苏大学 | Process for biodegrading yellow water of white spirit factory |
Non-Patent Citations (3)
| Title |
|---|
| Stable repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) from Aspergillus oryzae;Shakeri M等;《JOURNAL OF BIOSCIENCE AND BIOENGINEERING》;20080630;第105卷(第6期);第683页左栏第1段至第685页左栏第6段及表1 * |
| 白腐真菌处理难降解有机物的培养条件及应用研究;唐媛等;《世界科技研究与发展》;20080229;第30卷(第1期);第60-65页 * |
| 白腐真菌生物膜反应器处理染料生产废水实验研究;黄民生等;《上海环境科学》;20031231;第22卷(第7期);第2节，表1 * |
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