CN1271199C - High temperature Pleuotus nebrodensis Quel Tianshan 619 and cultivating method - Google Patents
High temperature Pleuotus nebrodensis Quel Tianshan 619 and cultivating method Download PDFInfo
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- CN1271199C CN1271199C CN 200410087862 CN200410087862A CN1271199C CN 1271199 C CN1271199 C CN 1271199C CN 200410087862 CN200410087862 CN 200410087862 CN 200410087862 A CN200410087862 A CN 200410087862A CN 1271199 C CN1271199 C CN 1271199C
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Abstract
The present invention relates to a new strain of bailing mushrooms, which is high-temperature bailing mushroom TianShan 619 and a cultivation method thereof. The high-temperature bailing mushroom TianShan 619 belongs to micro organism fungi. In the cultivation method, wild bailing mushroom samples are collected from the asafetida beach at the north slope of the TianShan in the XinJiang province; the high-temperature bailing mushroom TianShan 619 is obtained by using a tissue separating method through purification and high-temperature acclimation. The fruiting body of the high-temperature bailing mushroom TianShan 619 is large, white and in a horseshoe shape; the pileus diameter is from 6cm to 16cm, and the pileus thickness is from 3cm to 7cm; mycelia are white and have vigorous vital force; and spores are colorless, smooth and in a long oblong shape with the size of (8 to 14) mu m*(4-5.5) mu m. The cultivation method comprises the following steps: the preparation, the fermentation, the subpackage, the sterilization, the inoculation, the strain growth, outdoor move and field cultivation of mother seeds, original seed and cultivated seed culture media. The high-temperature bailing mushroom TianShan 619 has the advantages that fruiting can be realized without any low-temperature stimulation; the fruiting temperature range can vary between 7 DEG C and 30 DEG C; the high-temperature bailing mushroom TianShan 619 can be outdoors cultured in fields. The high-temperature bailing mushroom TianShan 619 has good mushroom shapes, good quality of mushrooms and high yield, and mushrooms can be harvested for two times in once plant; simultaneously, human power and material resources for building greenhouses and plastic tunnels are saved, and thereby, the economic benefit is high.
Description
One, technical field
The present invention relates to a kind of Pleurotus nebrodensis (the white spirit of formal name used at school is picked up the ears, the microorganism belonging to genus Mycophyta) bacterial classification and cultivating method.This bacterial classification need not low temperature stimulation, is fit at high temperature cultivate with outdoor land for growing field crops.
Two, technical background
Pleurotus nebrodensis is a kind of edible and medicinal fungi of preciousness, and thick, the pure white exquisiteness of the loose meat of its sporophore, taste aquatic foods, nutritious have very high edible and pharmaceutical use.Wild Pleurotus nebrodensis mainly grows in China desert Gobi desert, and very abominable because of its growing environment, excessive harvesting in recent years and livestock trample in addition, and wild Pleurotus nebrodensis is fewer and feweri.At this situation, the begin one's study artificial cultivation technique of Pleurotus nebrodensis of people.The patent application document that on April in 2000 5, State Intellectual Property Office announced that obtains by retrieval about Pleurotus nebrodensis, application number is 981198910, denomination of invention is " cultivation technique of Pleurotus nebrodensis ", and this patent application relates to the preparation and the sterilising method of substratum in the Pleurotus nebrodensis artificial culture.The disadvantage of this method and other Pleurotus nebrodensis cultivation techniques existence at present is: the first, must be through-5 ℃～5 ℃ low temperature stimulation ability fruiting; The second, the cultivation temperature must be controlled in 7 ℃～20 ℃ the scope, and in the most of area of China 1 year temperature remain on 7 ℃～20 ℃ fate seldom, this just makes Pleurotus nebrodensis to cultivate outdoor.If build greenhouse or plastic greenhouse in order to create cold condition, cost of investment and labour intensity have just been increased.
Three, summary of the invention
In order to overcome the above-mentioned shortcoming of prior art, the purpose of this invention is to provide a kind of low temperature stimulation that do not need and get final product fruiting, can be in outdoor field production, the fruiting temperature range is wide, less investment, Pleurotus nebrodensis new variety and cultivating method that output is high.
The Pleurotus nebrodensis high-temperature strain belongs to pleurotus (Pleurotus), the new bacterial strain of kind of (pleurotus nebrodensis) of picking up the ears.This bacterial strain has been deposited in Chinese typical culture collection center (being called for short CCTCC), preserving number CCTCC NO:M204073, preservation term 30 years on September 21st, 2004.It is a wild Pleurotus nebrodensis sample of gathering Xinjiang Tianshan north slope Resina Ferulae beach, adopts tissue isolation, forms through purification, high temperature acclimation.Its bacteria characteristic is: sporophore big, white, the shape of a hoof, and bacteria cover diameter 6cm-16cm, cap thickness 3cm-7cm, mycelium is pure white, and vitality is vigorous.Spore is colourless, smooth, oblong (8～14) μ m * (4～5.5) μ m.Need not low temperature stimulation and get final product fruiting, the fruiting temperature range can be at 7 ℃-30 ℃.
The cultivating method of Pleurotus nebrodensis high-temperature strain comprises: earlier cultivate female kind, original seed, cultivar according to ordinary method, make the cultivation material, to cultivate material then through building heap fermentation, pack, sterilization, inoculation, sending out bacterium, good bacterium bag moves to outdoor field production will to send out bacterium at last.
Absorb nutrient in the field soil better in order to help the bacterium bag, so that raising output, should be earlier that the land for growing field crops is smooth before the bacterium bag is imbedded the land for growing field crops, and propose water pouring the day before yesterday, again with the bacterium bag not the plastics bag of an end of fruiting peel 1/3rd off with blade, the end that will peel plastics bag then off places on the soil down, bag is close to mutually with bag, and tamp with soil in the slit between the bag.
Adopted technique scheme,, made the outdoor field production of Pleurotus nebrodensis become possibility because the Pleurotus nebrodensis high-temperature strain itself has the attribute of high temperature fruiting.And outdoor field production has reduced the required investment of construction warmhouse booth, because outdoor sunny, airflow is had friendly relations simultaneously, the land for growing field crops can provide sufficient nutrition to the bacterium bag, thereby the output height, and once cultivation can be gathered in the crops two batches, and economic benefit is increased substantially.
Four, embodiment
Below in conjunction with embodiment the present invention is further described.
The Pleurotus nebrodensis high-temperature strain, it is the pick up the ears new bacterial strain of kind of (pleurotus nebrodensis) of pleurotus (Pleurotus).Gather the wild Pleurotus nebrodensis sample of Xinjiang Tianshan north slope Resina Ferulae beach, adopt tissue isolation, form through purification, high temperature acclimation.Its bacteria characteristic is: sporophore big, white, the shape of a hoof, and bacteria cover diameter 6cm-16cm, cap thickness 3cm-7cm, mycelium is pure white, and vitality is vigorous.Spore is colourless, smooth, oblong (8～14) μ m * (4～5.5) μ m.
The cultivating method of Pleurotus nebrodensis high-temperature strain:
One 〉, female cultivation of planting:
It is the enriched medium of main developing medium that mother culture media adopts with potato, glucose, agar, with the mother culture media branch that the prepares test tube of packing into, sterilizes, inoculates, cultivates and can obtain female the kind.(above prescription and method of cultivation are known technology)
Two 〉, the cultivation of original seed and cultivar:
1, the prescription of substratum is: cotton seed hull 76-80%; (weight percent, as follows) starter 0.3～0.5%, wheat bran 14-16%, Semen Maydis powder 2～6%, sucrose 0.5～1.5%, terra alba 0.5～2%, lime powder 0.5～3%, P
H: 7-8.5.
2, compound method: with the siccative mixing and stirring in the above-mentioned prescription, back poach, make its culture material water content reach 60%, then through build heap fermentation, pack, sterilization, with female plant inoculate, temperature remains under 24 ℃-27 ℃ the constant temperature, sent out bacterium through 20-30 days and can obtain original seed.Prepare substratum same as described above again and can obtain cultivar after send out bacterium with the original seed inoculation.
Three 〉, the preparation of cultivation material:
1, the prescription of cultivation material:
Prescription one: cotton seed hull 77%, starter 0.3%, wheat bran 13.7%, Semen Maydis powder 5%, sucrose 1%, gypsum 1%, lime 2%, P
H: 7～8.5.
Prescription two: cotton seed hull 40%, wood sawdust 39%, starter 0.5%, wheat bran 13%, Semen Maydis powder 3%, sucrose 1.5%, gypsum 2%, lime 1%, P
H: 7～8.5.
Prescription three: cotton seed hull 35%, starter 0.4%, wheat bran 13.6%, Semen Maydis powder 7%, sucrose 0.5%, gypsum 0.5～2%, lime 3%, P
H: 7～8.5.
2, spice and build heap fermentation:
With the siccative mixing and stirring in the prescription, back poach, make the water content of culture material reach 60%, again stockpile is become wide 0.2m-1.5m, high 1.0m～1.2m, the long strip shape stockpile that length is not limit, with the wooden stick punching of 3cm-5cm, hole depth is the end of to around the stockpile, pitch of holes 0.4m～0.5m, with gunnysack or straw screen or mat stockpile is covered completely then, ferment.Turning after one day was turned over once in later per two days, turned over altogether 4～5 times, when culture material has strand wine flavour, the success of fermenting was described, regulated culture material water content to 60%, can pack.
3, pack and sterilization:
Cultivating bag adopts the polyethylene bag of 17cm * 33cm, 1 kilogram of every packed wet feed, and pocket is the degree of tightness unanimity up and down, and tighten with plastic ties at two, stays knot that can be undone by a pull, so that inoculation.The pot sterilization was advanced in pack on the same day same day.Adopt normal-pressure sterilization, after temperature rises to 100 ℃, kept 16 hours.
4, an inoculation and a bacterium:
Be seeded in the aseptic inoculation case and carry out, two inoculation, it is suitable that sack is tied up degree of tightness, after place 24 ℃～27 ℃ culturing room to send out bacterium in the bacterium bag.
5, plant fruiting outdoorly:
After the after-ripening of bacterium bag mycelia is finished, take aged bacterial classification piece off, use 1: 300 times the mushroom spirit of urging to spray two days later with tweezers.Then plastics bag is screwed in one remove by, treat that button grows to 2cm～3cm when big, move to outdoor field production.The outdoor cold bed of putting the bacterium bag during transplanting is smooth, proposes water pouring the day before yesterday, and the bacterium bag is moved on to outdoor cold bed.The bacterium bag of fruiting one end is not pulled down 1/3rd of outside plastics bag with blade, an end of fruiting not towards being placed down in the cold bed, bag with bag between to be close to, fill out wet soil in the space between bag.Build sunshade net fruiting on the cold bed, temperature is controlled at 7 ℃～30 ℃, and spray water 2-3 time with atomizer every day.From buddingging to plucking time is 7-10 days, can pluck two batches.
Claims (3)
1, Pleurotus nebrodensis high temperature bacterial strain, this bacterial strain is the pick up the ears new bacterial strain of kind of (pleurotusnebrodensis) of pleurotus (pleurotus), this bacterial strain has been preserved in Chinese typical culture collection center, preserving number CCTCC NO:M204073, it is characterized in that: it is the wild Pleurotus nebrodensis sample of gathering on the Xinjiang Tianshan north slope Resina Ferulae beach, adopts method for tissue separation to form through purification, high temperature acclimation; Its bacteria characteristic is: sporophore big, white, the shape of a hoof; Bacteria cover diameter 6cm-16cm, cap thickness 3cm-7cm, mycelium is pure white, and vitality is vigorous; Spore is colourless, smooth, oblong 8-14 μ m * 4～5.5 μ m, need not low temperature stimulation and gets final product fruiting, 7 ℃-30 ℃ of fruiting temperature ranges.
2, a kind of method of cultivating the said Pleurotus nebrodensis high temperature of claim 1 bacterial strain, it is to cultivate earlier female kind, original seed and cultivar successively, then culture material is stirred through mixing, add water, build heap fermentation, pack, sterilization, inoculation and send out bacterium, it is characterized in that: good bacterium bag shifts out outdoor field production fruiting will to send out bacterium.
3, according to the cultivating method of the said Pleurotus nebrodensis high temperature of claim 2 bacterial strain, it is characterized in that: when the said good bacterium bag of will sending out bacterium shifts out outdoor land for growing field crops, earlier that the land for growing field crops is smooth, and propose water pouring the day before yesterday, again with the bacterium bag not the plastics bag of an end of fruiting peel 1/3rd off with blade, the end that will peel plastics bag then off places on the soil down, bag is close to mutually with bag, and tamp with soil in the slit between the bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200410087862 CN1271199C (en) | 2004-10-24 | 2004-10-24 | High temperature Pleuotus nebrodensis Quel Tianshan 619 and cultivating method |
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| CN 200410087862 CN1271199C (en) | 2004-10-24 | 2004-10-24 | High temperature Pleuotus nebrodensis Quel Tianshan 619 and cultivating method |
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| CN1271199C true CN1271199C (en) | 2006-08-23 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104560734B (en) * | 2015-01-07 | 2017-09-01 | 中国农业科学院农业资源与农业区划研究所 | A kind of quality hard Pleurotus nebrodensis and its cultural method |
| CN104541979B (en) * | 2015-01-07 | 2017-07-18 | 中国农业科学院农业资源与农业区划研究所 | A kind of Pleurotus nebrodensis and its cultural method with short production cycle |
| CN104585060B (en) * | 2015-01-07 | 2016-08-24 | 中国农业科学院农业资源与农业区划研究所 | A kind of Pleurotus nebrodensis and cultural method thereof |
| CN104541978B (en) * | 2015-01-07 | 2017-03-15 | 中国农业科学院农业资源与农业区划研究所 | A kind of neat Pleurotus nebrodensis of fruiting and its cultural method |
| CN104585058B (en) * | 2015-01-07 | 2016-08-10 | 中国农业科学院农业资源与农业区划研究所 | Pleurotus nebrodensis that a kind of stem is short and cultural method thereof |
| CN105594457A (en) * | 2016-03-04 | 2016-05-25 | 常州市三新园艺有限公司 | Technology for cultivating pleuratus ferulae by aid of straw used as main cultivation base material |
| CN107258320A (en) * | 2017-06-27 | 2017-10-20 | 安徽诺亚农业有限公司 | A kind of Pleurotus nebrodensis high-yield cultivating method |
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