CN1257402C - Chemical lumination inspecting method and device for human body injury by X ray radiation - Google Patents
Chemical lumination inspecting method and device for human body injury by X ray radiation Download PDFInfo
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- CN1257402C CN1257402C CN 03113540 CN03113540A CN1257402C CN 1257402 C CN1257402 C CN 1257402C CN 03113540 CN03113540 CN 03113540 CN 03113540 A CN03113540 A CN 03113540A CN 1257402 C CN1257402 C CN 1257402C
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Abstract
The present invention provides a chemiluminescence detecting method for the injury degree of human bodies radiated by radioactive rays, which comprises the following steps: blood is extracted from a human body needs testing, and white cells in the blood are extracted to be put in a colorimetric utensil; the colorimetric utensil is put in a sample disc in a sample room; a chemiluminescence reagent is added in the colorimetric utensil to generate chemiluminescence; the chemiluminescence is selectively and fully automatically detected to obtain the light intensity value of the chemiluminescence; the light intensity of the chemiluminescence is compared with the light intensity of the known normal blood to obtain the corresponding result of the injury degree of the human body. A chemiluminescence detecting device for realizing the method comprises a photoelectric conversion assembly 2, a control assembly 5, a feedback assembly 10, a collection assembly 6, a display assembly 1, a computer 7, etc. The present invention has the advantages of simple operation, convenient use and high degree of automation. The present invention can rapidly and accurately evaluate the injury degree of human bodies, and can provide a forceful basis for diagnosing diseases and judging the health of human bodies.
Description
Technical field
The present invention relates to the chemiluminescence detection technology, particularly a kind ofly utilize chemiluminescence to detect to be subjected to body damaged degree methods and device thereof behind the radiation.
Background technology
Radiation cure (being called for short " radiotherapy ") still is the treatment early-stage cancer and alleviates one of main means of patient with advanced cancer misery at present, but the radiation flux on the just radiotherapy aperture that is calculated on the instrument of radiotherapy, and the suffered damage of patient's body does not have an objective appraisal standard after the radiotherapy, can only be to rely on attending doctor's experience to go to weigh, determine the dosage of next step radiotherapy then, be easy to generate bigger deviation like this, may have influence on the effect of treatment.In addition, unexpected nuclear leakage problem can not guarantee to avoid fully, and its influencing size and also can't weigh always personnel on every side.Also have, its healthy degree of personnel of working under the radioactive ray environment for a long time also can't quantitatively be judged always.The generation of above-mentioned this class problem all is because to so far, all also do not have both at home and abroad a kind of can check more exactly and measure can utilize by the technology of body damaged degree.On the other hand, find after deliberation, leucocyte in blood stress produce free radical after being subjected to ionising radiation, these free radicals can send stronger light with chemical illuminating reagent reaction, can weigh the measured and is subjected to the suffered degree of injury of body behind the radioactive ray irradiation by detecting this chemiluminescence.
Summary of the invention
The objective of the invention is to be subjected to the problem that the suffered degree of injury of body can't be weighed behind the radiation at existing, provide a kind of can be quick, objective, the chemical luminescence detection method of comprehensive assessment people suffered degree of injury of body after being subjected to radioactive ray irradiation exactly.
Another object of the present invention is to provide a kind of chemiluminescence detecting of realizing said method.
Purpose of the present invention is achieved through the following technical solutions: be subjected to originally that the chemical luminescence detection method of body damaged degree comprises the steps behind the radiation---
(1) leucocyte that extracts in the blood is put in the cuvette;
(2) cuvette is put into the sample disc of sample chamber;
(3) in cuvette, add chemical illuminating reagent, produce chemiluminescence;
(4) selectable full-automatic detection is carried out in chemiluminescence, obtained chemiluminescent light intensity value;
(5) chemiluminescent light intensity and known normal blood light intensity are contrasted the result who obtains corresponding body injury degree size.
In step (1), available lymph parting liquid separates blood, and the leucocyte of getting the middle layer adds to be put in the cuvette behind the nutrient solution to be measured.
In step (2), cuvette can be placed on arbitrarily in the sample disc; Can in sample disc, put into a plurality of cuvettes carry out tool optionally, measurement simultaneously.
In step (3), employed chemical illuminating reagent comprises MCLA, FCLA (MCLA, FCLA are two kinds and combine not isoplastic extra large firefly fluorescein analog), luminol or lucigenin.
In step (4), described full-automatic detection is for collecting photon by the programmed control optical fiber of computing machine and conducting, utilize the photon counting module to carry out optical detection, and light signal is converted to electric pulse, screen back outputting standard square wave (TTL) pulse, by the USB numbered card this pulse is counted, the corresponding chemiluminescent light intensity value of count results, and by computing machine output and storage.
The chemiluminescence detecting of realizing said method comprises photoelectric conversion component, Control Component, feedback component, acquisition component, display module, computing machine, photoelectric conversion component is electrically connected with acquisition component and display module simultaneously, and Control Component, feedback component, acquisition component are electrically connected with computing machine respectively.
Described photoelectric conversion component is used for the form output of photon number with the electric pulse number can be adopted the single photon counting module, as: the channel-type photon amplifier CPM-962 of PerkinElmer company.
Described Control Component be used for when automatically gathering to current institute collected specimens by turns, the control of steps such as the beginning of gathering and end; Can connect to form by controlled part such as stepper motor driver, relay control panel etc. in control card on the computing machine and the device; Realize installing the control of interior relay, stepper motor driver as the PCL-836 control card that can adopt Advantech company.
Described feedback component is used for the rotating disk of various product is positioned; Can realize that the feedback signal that it is exported is given computing machine, the measurement of being analyzed and being entered next step by computing machine by the control card component passes with infrared optical coupling device.
Described acquisition component is used for the collection of information, can use general data collecting card.The invention people for specialized designs of the present invention a kind of more succinct, usb data capture card easily, it is mainly formed by the EZ-USB AN2131QC exploitation of Cypress company, and has realized the drive controlling of LabVIEW to this card.
Described display module is to be used for showing collection result intuitively on device, can adopt NFC-1000C-1 multi-functional counter of Xinlian Electronic Equipment Co., Ltd., Nanjing and photoelectric conversion component to be electrically connected the result that promptly can show collection simply.
General virtual instrument software is housed in the described computing machine, the LabVIEW software of National Instruments company exploitation for example, be used to control and coordinate the operation of whole procedure, and can show the result of collection on computers continuously, can also the result who gather be handled and store.
The present invention has following characteristics and effect:
(1) radiological dose that this method was suitable for is 0～3Gy.
(2) owing to adopted this less sample cell of cuvette, sample size required when making test is very small, if fluid sample, as long as 200 μ l are so sampling is comparatively simple, convenient, little to the body injury degree.
(3) detection makes machine error almost nil to the influence of detector in the side with detector, and surveys with optical fiber light-guiding and supersensitive single photon counting mould, can improve signal to noise ratio (S/N ratio) and sensitivity significantly.
(4) different sample cells are carried out selecting measuring, can carry out the measurement of a plurality of samples simultaneously, thereby the gauger has bigger selection space, can save Measuring Time, and can compare measurement various product.
(5) the integrated degree height of apparatus of the present invention instrument, whole measuring process can automatically be finished and not need manually to intervene.
(6) apparatus of the present invention are simple to operate, and are easy to use, only need simply to be provided with the back and just can obtain required measurement result easily when measuring beginning.
(7) utilize the present invention can be quick, objective, comprehensive assessment people suffered degree of injury of body after being subjected to radioactive ray irradiation exactly, provide strong foundation for the diagnosis of disease and to the judgement of health.
Description of drawings
Fig. 1 is the block diagram that the present invention is subjected to the chemiluminescence detecting of body damaged degree behind the radiation;
Fig. 2 is the leukocytic chemiluminescence intensity figure that proposes in the blood of human body of the different exposure doses of process that detect with device shown in Figure 1.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing, but embodiments of the present invention are not limited thereto.
Embodiment
Of the present invention specifically as shown in Figure 1, as seen from Figure 1, apparatus of the present invention comprise display module 1, photoelectric conversion component 2, Control Component 5, acquisition component 6, feedback component 10, computing machine 7, photoelectric conversion component 2 is electrically connected with acquisition component 6 and display module 1 simultaneously, and Control Component 5, feedback component 10, acquisition component 6 are electrically connected with computing machine 7 respectively; Wherein Control Component 5 is connected and composed by stepper motor driver 5-1, relay control panel 5-2, control integrated circuit board 5-3; The light that sample in the cuvette 9 on the sample cell rotary-tray 3 sends is transferred to photoelectric conversion component 2 via optical fiber 8, passes to display module 1 and acquisition component 6 respectively after light signal is converted to electric pulse; The stepper motor that sample cell rotary-tray 3 is driven by stepper motor driver 5-1 drives and rotates, and the sample cell position that is placed with sample is measured; Beginning and end that relay control panel 5-2 control in the Control Component is gathered; The information that is collected by acquisition component 6 is passed to computing machine 7 and is handled and store; Member 4 is the darkroom, and 11 is crust of the device.The type selecting of each assembly of chemiluminescence detecting of the present invention is as follows: display module 1 adopts the NFC-1000C-1 multi-functional counter of Xinlian Electronic Equipment Co., Ltd., Nanjing; The CPM-962 type channel-type photon amplifier of the German PerkinElmer of photoelectric conversion component 2 employings company; Control integrated circuit board 5-3 in the Control Component selects the PCL-836 integrated circuit board of Advantech company for use; Acquisition component 6 is the independently developed usb data capture card of inventor, mainly is to utilize Cypress company EZ-USB AN2131QC chip to develop, and has realized the drive controlling of LabVIEW to this card; Optical fiber 8 is made by Nanjing spring brightness scientific ﹠ technical corporation, and adopting the single fiber diameter is the 35-40 micron, and logical optical cross-section is 5 millimeters a fibre bundle; The control of operation, demonstration and storing software are developed with the LabVIEW programming platform of National Instruments company on the computing machine 7.
Said apparatus is applied to whole blood is subjected to chemiluminescent detection after the various dose ionising radiation.50ml Type B whole blood is purchased in Jinan University Medical School first affiliated hospital, in this whole blood, add the lymph parting liquid, with the speed centrifuging of 2000r/m 10 minutes, the leucocyte in the middle of getting averaged in the branch glass Kolle flask that to install to 4 capacity be 25ml, and cell number is 6 * 10
6/ ml, the cell of every bottled 6ml; Contrast groups only contains the nutrient solution of equivalent, and without any cell.At room temperature use the PHOENIX of Canada's product of Jinan University Medical School first affiliated hospital
60The Co gamma-rays carries out the irradiation of various dose, and the productive rate of the radiation source of irradiation is 189.51cGy/min, is approximately 80cm to the distance of sample target; Through utilizing the inventive method to measure to its chemiluminescence at once after the irradiation.The MCLA that adds 8 μ M/L behind the measurement 75s, adding luminously behind the MCLA has bigger increasing, whether in order to check luminous is that free radical causes, in different samples, add superoxide dismutase (SOD), sweet mellow wine, sodium azide respectively and go to influence the chemiluminescence of MCLA, obtained result as shown in Figure 2.The result of Fig. 2 has shown the chemiluminescence of MCLA and the relation between the radiation dose: before not adding MCLA, chemiluminescence is very weak, after adding MCLA, control group has only small rising, but the luminous of other groups has tangible rising, and the amplitude that rises is raise by the increase of irradiation dose with blood.Can fast, objectively detect the suffered actual radiation amount of privileged site patient after radiotherapy of particular patient by present embodiment proof the inventive method; Apparatus of the present invention can be operated easily and be obtained result accurately.
Claims (9)
1, a kind ofly be subjected to the leukocytic chemical luminescence detection method in the body blood after the radioactive radiation, it is characterized in that:
(1) leucocyte that extracts in the blood is put in the cuvette;
(2) cuvette is put into the sample disc of sample chamber;
(3) in cuvette, add chemical illuminating reagent, produce chemiluminescence;
(4) selectable full-automatic detection is carried out in chemiluminescence, obtained chemiluminescent light intensity value.
2, according to claim 1ly be subjected to the leukocytic chemical luminescence detection method in the body blood after the radioactive radiation, it is characterized in that: with the lymph parting liquid blood is separated in step (1), the leucocyte of getting the middle layer adds to be put in the cuvette behind the nutrient solution to be measured.
3, according to claim 1ly be subjected to the leukocytic chemical luminescence detection method in the body blood after the radioactive radiation, it is characterized in that: the cuvette in step (2) is placed in the sample disc arbitrarily; In sample disc, put into a plurality of cuvettes carry out tool optionally, measurement simultaneously.
4, according to claim 1ly be subjected to the leukocytic chemical luminescence detection method in the body blood after the radioactive radiation, it is characterized in that: employed chemical illuminating reagent comprises MCLA, FCLA, luminol or lucigenin in step (3).
5, according to claim 1ly be subjected to the leukocytic chemical luminescence detection method in the body blood after the radioactive radiation, it is characterized in that: full-automatic detection the described in the step (4) to collecting photon by the programmed control optical fiber of computing machine and conducting, utilize the photon counting module to carry out optical detection, and light signal is converted to electric pulse, screen back outputting standard square-wave pulse, by the USB numbered card this pulse is counted, the corresponding chemiluminescent light intensity value of count results, and by computing machine output and storage.
6, a kind ofly be subjected to the leukocytic chemiluminescence detecting in the body blood after the radioactive radiation, it is characterized in that: comprise photoelectric conversion component, Control Component, feedback component, acquisition component, display module, computing machine; Photoelectric conversion component is electrically connected with acquisition component and display module simultaneously, and Control Component, feedback component, acquisition component are electrically connected with computing machine respectively.
7, according to claim 6ly be subjected to the leukocytic chemiluminescence detecting in the body blood after the radioactive radiation, it is characterized in that: described photoelectric conversion component is the single photon counting module.
8, according to claim 6ly be subjected to the leukocytic chemiluminescence detecting in the body blood after the radioactive radiation, it is characterized in that: described Control Component is connected to form by controlled part stepper motor driver, relay control panel in the control card on the computing machine and the device.
9, according to claim 6ly be subjected to the leukocytic chemiluminescence detecting in the body blood after the radioactive radiation, it is characterized in that: described feedback component is infrared optical coupling device.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 03113540 CN1257402C (en) | 2003-01-10 | 2003-01-10 | Chemical lumination inspecting method and device for human body injury by X ray radiation |
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| CN 03113540 CN1257402C (en) | 2003-01-10 | 2003-01-10 | Chemical lumination inspecting method and device for human body injury by X ray radiation |
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| CN1424575A CN1424575A (en) | 2003-06-18 |
| CN1257402C true CN1257402C (en) | 2006-05-24 |
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| CN107490432A (en) * | 2017-04-27 | 2017-12-19 | 安徽华脉科技发展有限公司 | A kind of Feebleness Light Signal Examining system |
| CN116297416B (en) * | 2023-05-22 | 2023-11-10 | 科美诊断技术股份有限公司 | Photo-excitation chemiluminescence detection device and detection method |
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