CN1257287C - 鸡常见传染病诊断性基因芯片及其用途 - Google Patents
鸡常见传染病诊断性基因芯片及其用途 Download PDFInfo
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- CN1257287C CN1257287C CN 200410078903 CN200410078903A CN1257287C CN 1257287 C CN1257287 C CN 1257287C CN 200410078903 CN200410078903 CN 200410078903 CN 200410078903 A CN200410078903 A CN 200410078903A CN 1257287 C CN1257287 C CN 1257287C
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Abstract
Description
试剂 | 预杂交液4ml用量 | 杂交液4ml用量 |
甲酰胺20×SSC50×Denhartd’s小牛胸腺DNA*光标样品核酸**1M PBS,PH6.4100mM EDTA20%硫酸葡聚糖钠 | 2.0ml1.0ml0.4ml0.2ml0.2ml0.2ml | 1.8ml1.0ml80ul80ul80-400ng80ul1.0ml |
HI | VI | PCR | 基因芯片法 | |
NDV | 42 | 35 | 60 | 54 |
AIV | 31 | 22 | 45 | 43 |
IBV | 24 | 18 | 51 | 47 |
ILTV | 7 | 12 | 10 | |
MDV | 9 | 23 | 20 | |
EDS-76V | 30 | 21 | 45 | 43 |
IBDV | 7 | 19 | 16 |
Claims (5)
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CN 200410078903 CN1257287C (zh) | 2002-12-12 | 2002-12-12 | 鸡常见传染病诊断性基因芯片及其用途 |
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CN 200410078903 CN1257287C (zh) | 2002-12-12 | 2002-12-12 | 鸡常见传染病诊断性基因芯片及其用途 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CNB021554080A Division CN1181210C (zh) | 2002-12-12 | 2002-12-12 | 家禽和/或家畜疫病诊断性基因芯片及其用途 |
Publications (2)
Publication Number | Publication Date |
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CN1616679A CN1616679A (zh) | 2005-05-18 |
CN1257287C true CN1257287C (zh) | 2006-05-24 |
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CN 200410078903 Expired - Lifetime CN1257287C (zh) | 2002-12-12 | 2002-12-12 | 鸡常见传染病诊断性基因芯片及其用途 |
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CN102851395B (zh) * | 2012-09-12 | 2013-09-18 | 中国检验检疫科学研究院 | 一种检测传染性喉气管炎病毒的液相芯片方法 |
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CN104232802B (zh) * | 2014-09-29 | 2016-04-27 | 四川农业大学 | 检测鸡传染性喉气管炎病毒、鸡新城疫病毒和鸡传染性支气管炎病毒的试剂盒 |
CN104293982B (zh) * | 2014-09-29 | 2016-11-16 | 四川农业大学 | 检测鸡新城疫病毒和鸡传染性支气管炎病毒的基因芯片以及试剂盒 |
CN104293979B (zh) * | 2014-09-29 | 2016-08-24 | 四川农业大学 | 鸡传染性支气管炎病毒和/或鸡传染性喉气管炎病毒的基因芯片以及试剂盒 |
CN110885899B (zh) * | 2018-09-10 | 2023-04-14 | 中国动物疫病预防控制中心(农业部屠宰技术中心) | 用于鉴别16种禽病病原冻干微芯片、试剂盒及方法 |
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