CN1256131C - Chinese traditional medicine preparation Qimaishen for treating viral myocarditis and its manufacturing method - Google Patents
Chinese traditional medicine preparation Qimaishen for treating viral myocarditis and its manufacturing method Download PDFInfo
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- CN1256131C CN1256131C CN 200310109972 CN200310109972A CN1256131C CN 1256131 C CN1256131 C CN 1256131C CN 200310109972 CN200310109972 CN 200310109972 CN 200310109972 A CN200310109972 A CN 200310109972A CN 1256131 C CN1256131 C CN 1256131C
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Abstract
The present invention provides a Chinese drug preparation with Radix Astragali, Radix Ophiopogonis and foreign ginseng for treating viral myocarditis. Astragalus roots, Radix Ophiopogonis and foreign ginseng or foreign ginseng leaves are proportionally combined or respectively decocted with water for extraction, and ethanol is used for precipitating removing impurities. Macroporous resin columns are used for refining the precipitated drugs which are then eluted with ethanol again, and eluent is filtered and prepared into injection after being collected. The Chinese drug preparation, which has the characteristics of obvious curative effect, reliability, safety, convenient application, etc., can adopt the forms of intravenous drip or vein bolus or muscle injection, and can be prepared into freeze-drying powdered injection solution, tablets, granular formulations, drop pills, medicinal granules, liquid preparations, powder agents, medicinal pills, capsules, suspending agents and solid dosage forms, such as spraying agents, aerosol, etc., for partial dosage. The medicine has the functions of supplementing qi, nourishing yin, supporting healthy energy to eliminate the evil, correcting the heart rate and stopping palpitation, and is used for treating cardiovascular diseases, such as viral myocarditis, coronary heart disease, angina pectoris, etc., due to qi and yin deficiency.
Description
Technical field:
The present invention relates to stilbene wheat ginseng Chinese medicine preparation for the treatment of viral myocarditis and preparation method thereof, and a kind of stilbene wheat ginseng injection is provided, belong to the Chinese medicine preparation technical field.
Background technology:
At present, viral myocarditis has become the commonly encountered diseases in medical science internal medicine field, and hazardness is very big, but does not still have the Chinese medicine preparation of drug administration by injection safely and effectively so far; Effective experience side clinically, flavour of a drug are more, and all adopt decoct oral, also do not have the Chinese medicine injection dosage form, and are slow to drug absorption for the more not only body of peroral dosage form, and use also inconvenient.Directly have influence on the therapeutic effect and the scope of application of the type medicine.
Radix Ginseng, the Radix Astragali, Radix Ophiopogonis be respectively as the wide model of medicinal application of diseases such as treatment cardiovascular etc., but clinically also not by by the Radix Astragali, Radix Ophiopogonis, Radix Panacis Quinquefolii three flavor Chinese medicines by a certain percentage proportioning form effective preparation for the treatment of viral myocarditis.
Summary of the invention:
It is fast to drug absorption that task of the present invention provides a kind of body, good effect.Easy to use, safe and reliable stilbene wheat ginseng Chinese medicine preparation and stilbene wheat ginseng injection.
Another task of the present invention provides the preparation method of above-mentioned Chinese medicine preparation and stilbene wheat ginseng injection.
Task of the present invention can reach by following measure:
The weight ratio of stilbene wheat ginseng injection raw material is the Radix Astragali: Radix Ophiopogonis: Radix Panacis Quinquefolii (or Folium Panacis Quinquefolii)=3.25: 2.5: 1; Contain in every 1ml injection of stilbene wheat ginseng injection and be equivalent to primary dose: Radix Astragali 1.3g, Radix Ophiopogonis 1.0g, Radix Panacis Quinquefolii 0.4g.
It is characterized in that it is to make Chinese medicine preparation by the crude drug of following portions by weight ratio: Radix Astragali 4-20, Radix Ophiopogonis 4-16, Radix Panacis Quinquefolii 4; The weight ratio of raw material is the Radix Astragali: Radix Ophiopogonis: Radix Panacis Quinquefolii (Folium Panacis Quinquefolii)=1-5: 1-4: 1.
The preparation method of stilbene wheat ginseng injection comprises two kinds of preparation methoies:
Method one: get the extraction that the Radix Astragali, Radix Ophiopogonis, Radix Panacis Quinquefolii (or stem and leaf of Radix Panacis Quinquefolii) are merged into the Xingqi effective ingredient
A) Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii (or stem and leaf of Radix Panacis Quinquefolii) merge to mix pulverized be coarse powder, with 0% to 95% pure liquid or aqueous solution, extract 1 time or once more than, get extracting solution;
B) merge each time extracting solution, concentrate concentrated solution or dry powder;
C) concentrated solution or dry powder add 0-100% alcohol, and is refining, filters, and gets filtrate;
D) with filtrate by macroporous adsorptive resins absorption I after, with low concentration alcohol eluting resin column, discard eluent, with 0%-90% concentration alcohol eluting, eluent;
E) after eluent passes through resin column II, decolorizing resin post III respectively, reclaim under reduced pressure alcohol, concentrated, dry, get dry powder;
F) will go up dry powder, be dissolved in water for injection, add a certain amount of medicinal carbon, reflux 10 minutes to 2 hours, cold room temperature is filtered to clear and brightly, and benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 1-10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets stilbene wheat ginseng injection.
Pure liquid described in the above-mentioned preparation method comprises other pure liquid solvents such as ethanol, methanol, butanols.
The total saponin content of stilbene wheat ginseng injection accounts for the 25-100% of injection dry; Total flavones accounts for 2-5%.(with the prescription of stem and leaf of Radix Panacis Quinquefolii, total flavones accounts for more than 6%).
Method two: get the Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii (or stem and leaf of Radix Panacis Quinquefolii) carry out the extraction of its effective ingredient respectively
A) be coarse powder with the Radix Astragali, Radix Ophiopogonis, Radix Panacis Quinquefolii (or stem and leaf of Radix Panacis Quinquefolii) pulverize separately, with 0% to 95% pure liquid or aqueous solution, extract 1 time or once more than, respectively Radix Astragali extractive solution, Radix Ophiopogonis extract and Radix Panacis Quinquefolii extracting solution;
B) merge each time extracting solution of Radix Astragali extractive solution, Radix Ophiopogonis extract and Radix Panacis Quinquefolii extracting solution respectively, concentrate respectively concentrated solution or dry powder; Respectively Radix Astragali concentrated solution, Radix Ophiopogonis concentrated solution and Radix Panacis Quinquefolii concentrated solution;
C) Radix Astragali concentrated solution, Radix Ophiopogonis, concentrated solution and Radix Panacis Quinquefolii concentrated solution or dry powder added 0-100% ethanol respectively, and is refining, filter, respectively Radix Astragali filtrate, Radix Ophiopogonis filtrate and Radix Panacis Quinquefolii filtrate;
D) respectively with Radix Astragali filtrate, Radix Ophiopogonis filtrate and Radix Panacis Quinquefolii filtrate, by behind the absorption with macroporous adsorbent resin post I,, discard eluent respectively with low concentration alcohol eluting resin column, with 0%-90% concentration alcohol eluting, respectively Radix Astragali eluent, Radix Ophiopogonis eluent and Radix Panacis Quinquefolii eluent;
E) respectively with Radix Astragali eluent, Radix Ophiopogonis eluent and Radix Panacis Quinquefolii eluent, respectively by behind resin column II, the decolorizing resin post III, reclaim under reduced pressure alcohol concentrates, dry, respectively Radix Astragali dry powder, Radix Ophiopogonis dry powder and Radix Panacis Quinquefolii dry powder;
F) with Radix Astragali dry powder, Radix Ophiopogonis dry powder, Radix Panacis Quinquefolii dry powder, be dissolved in water for injection, add a certain amount of medicinal carbon, reflux 30 minutes to 2 hours, cold room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 1-10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets stilbene wheat ginseng injection.
Pure liquid described in the above-mentioned preparation method comprises other pure liquid solvents such as ethanol, methanol, butanols.
The total saponin content of stilbene wheat ginseng injection accounts for the 25-100% of injection dry; Total flavones accounts for 2-5%.(with the prescription of stem and leaf of Radix Panacis Quinquefolii, total flavones accounts for more than 6%).
For step d) or e) the refining extraction dry powder that obtains of macroporous resin column, can be made into solid preparation such as drop pill, tablet, the watered pill, capsule etc.Also can mix by proper proportion with one or more pharmaceutically acceptable carriers or excipient, make the pharmaceutical composition that is applicable to the different dosage form that uses clinically. for example said compositions can be mixed with and can supply intravenous, the injection of drug administration by injection such as intramuscular, intraperitoneal, subcutaneous, marrowbrain intracavity is perhaps made the tablet, granule, drop pill, electuary, liquid preparation, the powder agent that are suitable for oral administration.Pill, capsule and suspending agent, and the spray, aerosol, cream, ointment and the suppository that are suitable for topical.
Preparation method three:
A) Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii (or stem and leaf of Radix Panacis Quinquefolii) merge to mix pulverized be coarse powder, with 0% to 95% pure liquid or aqueous solution, extract 1 time or once more than, get extracting solution;
B) merge each time extracting solution, concentrate concentrated solution or dry powder;
C) concentrated solution or dry powder add 0-100% alcohol, and is refining, filters, and gets filtrate;
D) with filtrate by macroporous adsorptive resins absorption I after, with low concentration alcohol eluting resin column, discard eluent, with 0%-90% concentration alcohol eluting, eluent;
E) after eluent passes through resin column II, decolorizing resin post III respectively, reclaim under reduced pressure alcohol, concentrated, dry, get dry powder;
F) with d) or e) dry powder, adds suitable excipient (starch etc.), make granule, add lubricant, be pressed into tablet with tablet machine, promptly get the stilbene wheat and join sheet.
The total saponin content of stilbene wheat ginseng sheet accounts for the 25-100% that tablet extracts; Total flavones accounts for 2-5%.(with the prescription of stem and leaf of Radix Panacis Quinquefolii, total flavones accounts for more than 6%).
Using method:
During intravenous drip, use each 10-30ml with 5-10% glucose injection or normal saline dilution back;
During intravenous injection, use each 5-10ml with the glucose injection dilution back of 5-50%;
The each 2-4ml of intramuscular injection, once-a-day.
The pharmacological effect experimental studies results finds that stilbene wheat ginseng injection can reduce the mortality rate of two kinds of animal patterns, improves unusual electrocardiogram, the rising of antagonism myocardium enzyme; Light microscopic and electron microscopic observation see that the cardiomyopathy infringement obviously alleviates, and medicine of the present invention can directly suppress the growth of Coxsackie virus, reduces neutralizing antibody level in the serum, also has arrhythmia, increase myocardial contraction, anti-myocardial ischemia, and effects such as antiinflammatory and enhancing humoral immunization.
Clinical drug determined curative effect of the present invention, show by treating 56 routine viral myocarditis results: after 2-3 course of therapy, treatment group total effective rate is 92.8%, with western medicine group relatively, two groups are being improved curative effects such as clinical symptoms, electrocardiogram relatively there were significant differences property (p＜0.05).
Good effect of the present invention is: as the Chinese medicine preparation of the drug administration by injection for the treatment of viral myocarditis, and reasonable recipe, determined curative effect, the injection back can produce tangible curative effect in 10-30 minute, did not see untoward reaction, and drug effect is quick, and is easy to use.It is a kind of medicine for the treatment of viral myocarditis safely and effectively.
The specific embodiment:
The following example is intended to further describe for example the present invention, rather than limit the present invention by any way, under the prerequisite that does not deviate from the spirit and principles in the present invention, any change or change that the ability city those of ordinary skill that the present invention did is realized easily all will fall within the claim scope that awaits the reply of the present invention.
Embodiment 1:
A. prepare each extract of the Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii
(1). get Radix Astragali 13kg, Radix Ophiopogonis 10kg, Radix Panacis Quinquefolii 4kg pulverize separately is a coarse powder, adds 20 times water respectively, heated and boiled three times, each 1 hour;
(2) Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii merge three extracting solution separately respectively, reclaim ethanol, concentrate, and concentrated solution, dilution, filter: collection merges the Radix Astragali, Radix Ophiopogonis and the Radix Panacis Quinquefolii water extract of gained separately respectively, and being concentrated into 50 ℃ of relative densities respectively is 1.10;
(3) respectively with the Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii concentrated solution separately, add ethanol to determining alcohol respectively and reach 40%, placed respectively 24 hours, filter, filtrate recycling ethanol, to there not being the alcohol flavor, dilution is filtered;
(4) respectively the filtrate of the Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii is adsorbed (D21 type) by macroporous adsorptive resins respectively, respectively with 10% ethanol-eluting resin column be eluted to eluent colourless after, discard eluent, use 75% concentration ethanol eluting resin column more respectively, collect eluent separately respectively;
(5) 75% ethanol elution of the Radix Astragali, Radix Ophiopogonis and Radix Panacis Quinquefolii is respectively by cationic resin column and resin anion (R.A.) post, and reclaim under reduced pressure alcohol is condensed into extractum shape or dry powder respectively; Respectively Radix Astragali dry powder, Radix Ophiopogonis dry powder and Radix Panacis Quinquefolii dry powder;
B. prepare stilbene wheat ginseng injection
Get Radix Astragali dry powder, Radix Ophiopogonis dry powder, Radix Panacis Quinquefolii dry powder merges, be dissolved in water for injection, add 0.05% active carbon (by the dosing volume calculation), boiled 30 minutes, cold room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets 10,000 ml stilbene wheats ginseng injection.
Embodiment 2:
A. prepare the Radix Astragali, Radix Ophiopogonis and American ginseng total extract
(1). get Radix Astragali 13kg, Radix Ophiopogonis 10kg, Radix Panacis Quinquefolii 4kg and merge to mix to pulverize and be coarse powder, add 5 times water, heated and boiled three times, each 1 hour.
(2) the water extract of collection and merging gained, being concentrated into 80 ℃ of relative densities is 1.10;
(3) concentrated solution adds ethanol to determining alcohol and reaches 90%, places 24 hours, filter, and filtrate recycling ethanol, to there not being the alcohol flavor, dilution is filtered.
(4) with filtrate by macroporous adsorptive resins absorption (D21 type), use 20% ethanol-eluting resin column, be eluted to eluent colourless after, discard eluent, reuse 50% concentration ethanol eluting resin column, collection eluent;
(5) 50% ethanol elution are by cationic resin column and resin anion (R.A.) post, and reclaim under reduced pressure alcohol is condensed into extractum shape or dry powder;
B. prepare stilbene wheat ginseng injection
Get the Radix Astragali, Radix Ophiopogonis and American ginseng total extract dry powder, be dissolved in water for injection, add 1% active carbon (by the dosing volume calculation), boiled 30 minutes, cold room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets 10,000 ml stilbene wheats ginseng injection.
Embodiment 3:
A. prepare the Radix Astragali, Radix Ophiopogonis and Folium Panacis Quinquefolii total extract
(1). get Radix Astragali 13kg, Radix Ophiopogonis 10kg, Radix Panacis Quinquefolii 4kg and merge to mix to pulverize and be coarse powder, add 30 times water, heated and boiled 5 times, each 0.5 hour.
(2) the water extract of collection and merging gained, being concentrated into 50 ℃ of relative densities is 1.10;
(3) concentrated solution adds ethanol to determining alcohol and reaches 60%, places 24 hours, filter, and filtrate recycling ethanol, to there not being the alcohol flavor, dilution is filtered.
(4) filtrate is adsorbed by macroporous adsorptive resins, uses 20% ethanol-eluting resin column, be eluted to eluent colourless after, discard eluent, reuse 60% concentration ethanol eluting resin column is collected eluent;
(5) 60% ethanol elution reclaim alcohol by cationic resin column and resin anion (R.A.) post, are condensed into extractum shape or dry powder;
B. prepare stilbene wheat ginseng injection
Get the Radix Astragali, Radix Ophiopogonis and Folium Panacis Quinquefolii total extract dry powder, be dissolved in water for injection, add 0.7% active carbon (by the dosing volume calculation), boiled 30 minutes, cold room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets 10,000 ml stilbene wheats ginseng injection.
Embodiment 4:
A. prepare the Radix Astragali, Radix Ophiopogonis and Folium Panacis Quinquefolii extract
(1). get Radix Astragali 13kg, Radix Ophiopogonis 10kg, Radix Panacis Quinquefolii 4kg pulverize separately is a coarse powder, adds 30 times water respectively, heated and boiled three times, each 1 hour.
(2) Radix Astragali, Radix Ophiopogonis and Folium Panacis Quinquefolii merge each time extracting solution respectively, reclaim ethanol, concentrate, and concentrated solution, dilution, filter: collection merges the Radix Astragali, Radix Ophiopogonis and the Folium Panacis Quinquefolii water extract of gained separately respectively, and being concentrated into 50 ℃ of relative densities respectively is 1.10;
(3) respectively with the Radix Astragali, Radix Ophiopogonis and Folium Panacis Quinquefolii concentrated solution separately, add ethanol to determining alcohol respectively and reach 50%, placed respectively 24 hours, filter, filtrate recycling ethanol, to there not being the alcohol flavor, dilution is filtered.
(4) respectively the filtrate of the Radix Astragali, Radix Ophiopogonis and Folium Panacis Quinquefolii is adsorbed (D21 type) by macroporous adsorptive resins respectively, respectively with 20% ethanol-eluting resin column be eluted to eluent colourless after, discard eluent, use 65% concentration ethanol eluting resin column more respectively, collect eluent separately respectively;
(5) 65% ethanol elution of the Radix Astragali, Radix Ophiopogonis and Folium Panacis Quinquefolii is respectively by cationic resin column and resin anion (R.A.) post, and reclaim under reduced pressure alcohol is condensed into extractum shape or dry powder respectively; Respectively Radix Astragali dry powder, Radix Ophiopogonis dry powder and Folium Panacis Quinquefolii dry powder;
B. prepare stilbene wheat ginseng injection
Get Radix Astragali dry powder, Radix Ophiopogonis dry powder, Folium Panacis Quinquefolii dry powder merges, be dissolved in water for injection, add 2% active carbon (by the dosing volume calculation), boiled 30 minutes, cold room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets 10,000 ml stilbene wheats ginseng injection.
Embodiment 5:
A. prepare the Radix Astragali, Radix Ophiopogonis and American ginseng total extract
(1). get Radix Astragali 19kg, Radix Ophiopogonis 15kg, Radix Panacis Quinquefolii 4kg and merge to mix to pulverize and be coarse powder, add 5 times water, heated and boiled three times, each 1 hour.
(2) the water extract of collection and merging gained, being concentrated into 80 ℃ of relative densities is 1.10;
(3) concentrated solution adds ethanol to determining alcohol and reaches 90%, places 24 hours, filter, and filtrate recycling ethanol, to there not being the alcohol flavor, dilution is filtered.
(4) with filtrate by macroporous adsorptive resins absorption (D21 type), use 20% ethanol-eluting resin column, be eluted to eluent colourless after, discard eluent, reuse 50% concentration ethanol eluting resin column, the collection eluent is condensed into dry powder;
(5) get dry powder 1kg, medical starch 0.5kg, with 80% ethanol liquid wet granulation, granulate, dress 1# capsule, every 0.2g.
Other project should meet 2000 editions capsule projects of Pharmacopoeia of People's Republic of China relevant requirements.
Embodiment 6:
Get the dry powder 1kg that (5) item makes in embodiment 1 or 2, make drop pill by the drop pill routine fashion, every 0.2g.
Other project should meet 2000 editions drop pill projects of Pharmacopoeia of People's Republic of China relevant requirements.
Embodiment 7
Get the dry powder 1kg that (5) item makes in embodiment 3 or 4, be dissolved in water for injection, add 2% active carbon (by the dosing volume calculation), boiled 30 minutes, cold room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 10%NaOH solution, coarse filtration, fine straining, sterilization, lyophilizing makes.
Other project should meet 2000 editions lyophilized injectable powder projects of Pharmacopoeia of People's Republic of China relevant requirements.
Claims (7)
1, a kind of Chinese medicine preparation for the treatment of viral myocarditis is characterized in that being made by following parts by weight proportion raw material:
Radix Astragali 4-20, Radix Ophiopogonis 4-16, Radix Panacis Quinquefolii 4.
2, a kind of Chinese medicine injection for the treatment of viral myocarditis is characterized in that: contain in every 1ml injection be equivalent to former in dose: Radix Astragali 1.3g, Radix Ophiopogonis 1.0g, Radix Panacis Quinquefolii 0.4g.
3, medicine according to claim 1 and 2 is characterized in that: Radix Panacis Quinquefolii can be replaced by stem and leaf of Radix Panacis Quinquefolii or Radix Ginseng, Stem and leaf of Radix Ginseng.
4, a kind of preparation method of stilbene wheat ginseng injection for the treatment of viral myocarditis is characterized in that: get the extraction that the Radix Astragali, Radix Ophiopogonis, Radix Panacis Quinquefolii or the Radix Astragali, Radix Ophiopogonis, stem and leaf of Radix Panacis Quinquefolii are merged into the Xingqi effective ingredient
A) Radix Astragali, Radix Ophiopogonis, Radix Panacis Quinquefolii or the Radix Astragali, Radix Ophiopogonis, stem and leaf of Radix Panacis Quinquefolii merged to mix to pulverize be coarse powder, with 0% to 95% pure liquid or aqueous solution, extract 1 time or once more than, get extracting solution;
B) merge each time extracting solution, concentrate concentrated solution or dry powder;
C) concentrated solution or dry powder add 0-100% alcohol, and is refining, filters, and gets filtrate;
D) with filtrate by macroporous adsorptive resins absorption I after, with low concentration alcohol eluting resin column, discard eluent, with 0%-90% concentration alcohol eluting, eluent;
E) after eluent passes through resin column II, decolorizing resin post III respectively, reclaim under reduced pressure alcohol, concentrated, dry, get dry powder;
F) will go up dry powder, be dissolved in water for injection, add a certain amount of medicinal carbon, reflux 30 minutes to 2 hours, be chilled to room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 1-10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets stilbene wheat ginseng injection.
5, a kind of preparation method for the treatment of the stilbene wheat ginseng injection of viral myocarditis is characterized in that:
Get the Radix Astragali, Radix Ophiopogonis, Radix Panacis Quinquefolii or the Radix Astragali, Radix Ophiopogonis, stem and leaf of Radix Panacis Quinquefolii carry out the extraction of its effective ingredient respectively
A) be coarse powder with the Radix Astragali, Radix Ophiopogonis, Radix Panacis Quinquefolii or the Radix Astragali, Radix Ophiopogonis, stem and leaf of Radix Panacis Quinquefolii pulverize separately, with 0% to 95% pure liquid or aqueous solution, extract 1 time or once more than, respectively Radix Astragali extractive solution, Radix Ophiopogonis extract and Radix Panacis Quinquefolii extracting solution;
B) merge each time extracting solution of Radix Astragali extractive solution, Radix Ophiopogonis extract and Radix Panacis Quinquefolii extracting solution respectively, concentrate respectively concentrated solution or dry powder; Respectively Radix Astragali concentrated solution, Radix Ophiopogonis concentrated solution and Radix Panacis Quinquefolii concentrated solution;
C) Radix Astragali concentrated solution, Radix Ophiopogonis, concentrated solution and Radix Panacis Quinquefolii concentrated solution or dry powder added 0-100% ethanol respectively, and is refining, filter, respectively Radix Astragali filtrate, Radix Ophiopogonis filtrate and Radix Panacis Quinquefolii filtrate;
D) respectively with Radix Astragali filtrate, Radix Ophiopogonis filtrate and Radix Panacis Quinquefolii filtrate, by behind the absorption with macroporous adsorbent resin post I,, discard eluent respectively with low concentration alcohol eluting resin column, with 0%-90% concentration alcohol eluting, respectively Radix Astragali eluent, Radix Ophiopogonis eluent and Radix Panacis Quinquefolii eluent;
E) respectively with Radix Astragali eluent, Radix Ophiopogonis eluent and Radix Panacis Quinquefolii eluent, respectively by behind resin column II, the decolorizing resin post III, reclaim under reduced pressure alcohol concentrates, dry, respectively Radix Astragali dry powder, Radix Ophiopogonis dry powder and Radix Panacis Quinquefolii dry powder:
F) with Radix Astragali dry powder, Radix Ophiopogonis dry powder, Radix Panacis Quinquefolii dry powder, be dissolved in water for injection, add a certain amount of medicinal carbon, reflux 30 minutes to 2 hours, be chilled to room temperature, be filtered to clear and brightly, benefit adds to the full amount of water for injection, and transfers PH to 7-7.5 with 1-10%NaOH solution, coarse filtration, fine straining, sterilization promptly gets stilbene wheat ginseng injection.
6, according to claim 4 or 5 described preparation methoies, it is characterized in that: Radix Panacis Quinquefolii can be replaced by stem and leaf of Radix Panacis Quinquefolii or Radix Ginseng, Stem and leaf of Radix Ginseng.
7, according to claim 4 or 5 described preparation methoies, it is characterized in that: for the refining extraction dry powder that obtains of concentrated solution, can be made into lyophilized injectable powder, tablet, granule, drop pill, electuary, liquid preparation, powder agent, pill, capsule and suspending agent on the pharmaceutics, and the spray, the aerosol solid dosage forms that are suitable for topical.
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| CN 200310109972 CN1256131C (en) | 2003-11-05 | 2003-11-05 | Chinese traditional medicine preparation Qimaishen for treating viral myocarditis and its manufacturing method |
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| CN102293914A (en) * | 2011-01-30 | 2011-12-28 | 辽宁中医药大学 | Traditional Chinese medicine for treating viral myocarditis and arrhythmia and preparation method thereof |
| CN103520443B (en) * | 2013-10-22 | 2015-06-10 | 宋彬 | Traditional Chinese medicinal preparation for treating adult viral myocarditis and preparation method for traditional Chinese medicinal preparation |
| CN105079438B (en) * | 2015-09-11 | 2018-08-10 | 山东大学 | A kind of pharmaceutical preparation for treating vital myocarditis |
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