CN115364179A - Traditional Chinese medicine composition for reducing blood uric acid and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for reducing blood uric acid and preparation method and application thereof Download PDFInfo
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- CN115364179A CN115364179A CN202211043216.7A CN202211043216A CN115364179A CN 115364179 A CN115364179 A CN 115364179A CN 202211043216 A CN202211043216 A CN 202211043216A CN 115364179 A CN115364179 A CN 115364179A
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- uric acid
- reducing blood
- blood uric
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- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 229940116269 uric acid Drugs 0.000 title claims abstract description 57
- 239000003814 drug Substances 0.000 title claims abstract description 56
- 210000004369 blood Anatomy 0.000 title claims abstract description 38
- 239000008280 blood Substances 0.000 title claims abstract description 38
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- 238000002360 preparation method Methods 0.000 title claims abstract description 20
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- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims abstract description 9
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Classifications
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/536—Prunella or Brunella (selfheal)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
-
- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8994—Coix (Job's tears)
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
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- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A—HUMAN NECESSITIES
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- A61P19/00—Drugs for skeletal disorders
- A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The invention discloses a traditional Chinese medicine composition for reducing blood uric acid and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition for reducing blood uric acid comprises the following raw materials in parts by weight: 600-900 parts of selfheal, 10-50 parts of coptis chinensis, 10-50 parts of bighead atractylodes rhizome, 5-30 parts of coix seed, 5-30 parts of achyranthes root and 5-30 parts of liquorice. According to the invention, selfheal is used as a monarch drug, coptis chinensis, bighead atractylodes rhizome and coix seeds are used as ministerial drugs, achyranthes bidentata is used as an adjuvant drug, and liquorice is used as a conductant drug, and all the components are matched with each other, so that the blood uric acid concentration is effectively reduced, and the traditional Chinese medicine has a wide application prospect in the aspects of preventing and treating gout.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for reducing blood uric acid, and a preparation method and application thereof.
Background
Gout (gout) is a group of clinical syndromes caused by deposition of uric acid at joints and the like in vivo due to purine metabolic disorder or reduction of uric acid excretion in vivo, and continuous hyperuricemia is a necessary condition for inducing gout. Uric acid is a product of purine metabolism, has limited solubility in body fluid, causes hyperuricemia in vivo or decreases uric acid excretion when purine metabolism is disturbed or purine ingestion is excessive, and causes uric acid accumulated in body fluid to exceed its saturation concentration, and easily precipitates in tissues, causing hyperuricemia (hyperuricemia), and about 5-12% of patients with hyperuricemia finally develop gout. Hyperuricemia is the most important biochemical basis of gout and is the most dangerous factor for inducing gout, so that the control of uric acid level is the key for preventing and reducing blood uric acid, and the reduction of blood uric acid concentration through medicines is an effective method for preventing gout.
The existing uric acid lowering drugs comprise two categories of drugs for promoting uric acid excretion and drugs for inhibiting uric acid synthesis, wherein the drugs for promoting uric acid excretion are represented by benzbromarone (benzbromarone), but are often accompanied by side effects such as rash, fever, kidney damage and the like; drugs that inhibit uric acid synthesis act by acting on xanthine oxidase (xanthine oxidase), a key enzyme in the process of uric acid production, and the most clinically used xanthine oxidase inhibitor is allopurinol; allopurinol has definite curative effect, however, about 2 percent of patients are allergic to allopurinol, and about 3 to 10 percent of patients have adverse reactions in the treatment process, can induce toxic hepatitis and have adverse reactions such as gastrointestinal reaction, leukopenia and thrombocytopenia and the like. Therefore, the research on high-efficiency anti-gout drugs is urgent.
Disclosure of Invention
The invention discloses a traditional Chinese medicine composition for reducing blood uric acid and a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a traditional Chinese medicine composition for reducing blood uric acid comprises the following raw materials in parts by weight:
600-900 parts of selfheal, 10-50 parts of coptis chinensis, 10-50 parts of bighead atractylodes rhizome, 5-30 parts of coix seed, 5-30 parts of achyranthes root and 5-30 parts of liquorice.
The Chinese medicinal composition of the invention uses prunella vulgaris and has the effective component of rosmarinic acid. Modern researches show that after a human body takes purine substances, uric acid is required to be generated under the action of xanthine oxidase, and rosmarinic acid has an inhibitory effect on the xanthine oxidase so as to inhibit the generation of the uric acid. The content of rosmarinic acid in the selfheal specified by Chinese pharmacopoeia is not less than 0.2 percent, and researches show that the content of rosmarinic acid in the selfheal in different production places can reach 0.2 to 2.3 percent. The invention takes the selfheal containing rosmarinic acid as the monarch drug in the formula; rhizoma coptidis, rhizoma atractylodis macrocephalae and semen coicis are matched as the ministerial drugs of the prescription; coptis root, rhizoma Coptidis, bitter in flavor and cold in nature, is used to clear heat and to dry dampness; bighead atractylodes rhizome, rhizoma atractylodis macrocephalae, which is used for tonifying spleen and qi, eliminating dampness and promoting diuresis, is mainly used for treating spleen deficiency, anorexia and edema; the coix seeds have the effects of excreting dampness, strengthening spleen, relaxing muscles and tendons and removing arthralgia; the three medicines are combined, and can be matched with selfheal to effectively reduce the concentration of blood uric acid and relieve the pain of meridians and joints. Achyranthes root, radix Achyranthis bidentatae, as an adjuvant drug in the recipe, has the effects of activating blood and stimulating the menstrual flow, and strengthening tendons and bones. So Licorice root, radix Glycyrrhizae can coordinate the actions of the other drugs in the recipe, relieving spasm and alleviating pain. The traditional Chinese medicine composition has compatible medicines, has the effects of clearing heat and purging fire, promoting diuresis and excreting dampness, strengthening muscles and bones and benefiting joints, and can be used for treating gout with symptoms of joint swelling, hot pain, limited activity and the like caused by damp-heat accumulation.
The preparation method of the traditional Chinese medicine composition for reducing blood uric acid comprises the following steps:
(1) Reflux-extracting Prunellae Spica with ethanol solution to obtain Prunellae Spica ethanol extractive solution;
(2) Recovering ethanol from the ethanol extract of the selfheal, concentrating and purifying by using macroporous resin;
(3) Spray drying the purified extract to obtain powder 1 containing rosmarinic acid;
(4) Pulverizing part of Atractylodis rhizoma and Coicis semen to obtain powder 2;
(5) Decocting Coptidis rhizoma, achyranthis radix, glycyrrhrizae radix and the rest Atractylodis rhizoma and Coicis semen in water, filtering the decoction, concentrating the filtrate into paste, drying, and pulverizing to obtain powder 3;
(6) Mixing powder 1, powder 2 and powder 3.
The method adopts the combination of ethanol solution extraction and macroporous resin adsorption to extract and purify the traditional Chinese medicine selfheal rich in rosmarinic acid, so that the content of the rosmarinic acid in the extract can reach 10-20%, and the traditional Chinese medicine selfheal rich in rosmarinic acid is matched with medicinal materials such as coptis chinensis, coix seeds, bighead atractylodes rhizome and the like, so that the xanthine oxidase activity can be effectively inhibited, the concentration of blood uric acid is reduced, and the effect of inhibiting the occurrence and development of gout is achieved.
According to the invention, part of the bighead atractylodes rhizome and the coix seed are directly crushed into the medicine, wherein the bighead atractylodes rhizome contains volatile components such as atractylone and the like, and part of the volatile active components are reserved after the bighead atractylodes rhizome and the coix seed are crushed into the medicine; the coix seed medicinal material has stronger powder property, and the addition of auxiliary materials in the preparation can be reduced by crushing the coix seed medicinal material into the preparation.
The coptis, the achyranthes, the liquorice and other medicines are extracted and used as medicines by a water decoction method, most of the active ingredients have better solubility in water, and the full extraction of the active ingredients can be ensured.
Preferably, in the step (1), ethanol solution with mass fraction of 40-95% is used for reflux extraction for 1-3 times, each time for 0.5-2.5 hours, and the amount of ethanol solution used for reflux extraction for each time is 4-10 times of the weight of selfheal.
Preferably, in the step (2), recovering ethanol from the ethanol extract of the selfheal until no alcohol smell exists, and concentrating; passing through D101 macroporous resin, washing with water, discarding water washing solution, eluting with 20-80% ethanol solution, collecting ethanol eluate, and recovering ethanol to obtain purified extract.
Preferably, the amount of water and the amount of 20-80% ethanol solution are 4 column volumes.
Preferably, in the step (3), the purified extract is added with starch and beta-cyclodextrin, and spray drying is carried out, wherein the air inlet temperature of drying is 150-160 ℃, the air exhaust temperature is 80-90 ℃, the liquid inlet volume flow is 15mL/min, and the atomizing airflow pressure is 220Pa.
Preferably, the amount of starch is 10% of the mass of the purified extract and the amount of beta-cyclodextrin is 10-15% of the mass of the purified extract.
Preferably, in the step (4), the dosage of the bighead atractylodes rhizome and the coix seed is one third of the dosage of the prescription; pulverizing, and sieving with 80 mesh sieve.
Preferably, in the step (5), water is added for 2 times, 1.5 hours each time, and the amount of the added water is 6-8 times of the weight of the medicinal materials; mixing decoctions, filtering, concentrating the filtrate to obtain soft extract with relative density of 1.25-1.30 at 80 deg.C, controlling vacuum degree at 0.08MPa or less and temperature at 70-80 deg.C, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve.
The traditional Chinese medicine composition or the traditional Chinese medicine composition prepared by the method is applied to preparing medicines for reducing blood uric acid and preventing or treating gout.
In conclusion, the invention takes the selfheal as the monarch drug, the coptis root, the bighead atractylodes rhizome and the coix seed as the ministerial drugs, the achyranthes root as the adjuvant drug and the liquorice as the conductant drug, and the selfheal, the atractylodes rhizome, the coix seed and the achyranthes root are matched with each other, so that the concentration of the blood uric acid is effectively reduced, and the invention has wide application prospect in the aspects of preventing and treating gout.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
A traditional Chinese medicine composition for reducing blood uric acid comprises the following raw materials:
750g of selfheal, 30g of coptis chinensis, 30g of bighead atractylodes rhizome, 15g of coix seed, 15g of achyranthes bidentata and 15g of liquorice.
The preparation method of the traditional Chinese medicine composition for reducing blood uric acid comprises the following steps:
(1) Taking selfheal, extracting by refluxing with 70% ethanol solution for 2 times, 1 hour each time, wherein the amount of the ethanol solution extracted each time is 6 times of the weight of the selfheal; extracting, filtering, and mixing filtrates to obtain Prunellae Spica ethanol extractive solution.
(2) Recovering ethanol from the selfheal ethanol extracting solution until no ethanol smell exists, and concentrating the solution until the mass ratio of the solution is 1; passing through D101 macroporous resin, washing with 4 times column volume of water, discarding water washing solution, eluting with 4 times column volume of 50% ethanol solution, collecting ethanol eluate, and recovering ethanol to obtain purified extract.
(3) Adding starch and beta-cyclodextrin into the purified extract, and spray drying to obtain powder 1 containing rosmarinic acid;
the drying air inlet temperature is controlled to be 150-160 ℃, the air exhaust temperature is controlled to be 80-90 ℃, the volume flow of the inlet liquid is 15mL/min, and the pressure of the atomizing air flow is 220Pa. The amount of starch used was 10% of the mass of the purified extract and the amount of beta-cyclodextrin used was 10% of the mass of the purified extract.
(4) One third of the prescription weight of the rhizoma atractylodis macrocephalae and semen coicis is taken, crushed and sieved by a 80-mesh sieve to obtain powder 2.
(5) Decocting Coptidis rhizoma, achyranthis radix, glycyrrhrizae radix and the rest Atractylodis rhizoma and Coicis semen in water for 1.5 hr for 2 times, wherein the water addition amount is 8 and 6 times of the weight of the medicinal materials respectively; mixing decoctions, filtering, concentrating the filtrate to relative density of 1.25-1.30 (measured at 80 deg.C), drying the obtained soft extract under reduced pressure at vacuum degree of 0.08MPa or less and temperature of 70-80 deg.C to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain powder 3.
(6) Mixing powder 1, powder 2 and powder 3.
The Chinese medicinal composition can be made into pill, capsule or tablet, and can be used for reducing blood uric acid and preventing and treating gout.
Example 2
A traditional Chinese medicine composition for reducing blood uric acid comprises the following raw materials:
800g of selfheal, 20g of coptis root, 40g of bighead atractylodes rhizome, 10g of coix seed, 10g of achyranthes root and 25g of liquorice.
The preparation method of the traditional Chinese medicine composition for reducing blood uric acid comprises the following steps:
(1) Taking selfheal, and extracting by refluxing 75% of ethanol solution for 3 times, wherein the amount of the ethanol solution extracted each time is 4 times of the weight of the selfheal, and each time is 0.5 hour; extracting, filtering, and mixing filtrates to obtain Prunellae Spica ethanol extractive solution.
(2) Recovering ethanol from the selfheal ethanol extracting solution until no ethanol smell exists, and concentrating the solution until the mass ratio of the solution is 1; passing through D101 macroporous resin, washing with 4 times column volume of water, discarding water washing solution, eluting with 4 times column volume of 70% ethanol solution, collecting ethanol eluate, and recovering ethanol to obtain purified extract.
(3) Adding starch and beta-cyclodextrin into the purified extract, and spray drying to obtain powder 1 containing rosmarinic acid;
the drying air inlet temperature is controlled to be 150-160 ℃, the air exhaust temperature is controlled to be 80-90 ℃, the volume flow of the inlet liquid is 15mL/min, and the pressure of the atomizing air flow is 220Pa. The amount of starch was 10% and the amount of beta-cyclodextrin 15% of the purified extract.
(4) Taking one third of the bighead atractylodes rhizome and the coix seed according to the weight of the prescription, crushing and sieving by a 80-mesh sieve to obtain powder 2.
(5) Decocting Coptidis rhizoma, achyranthis radix, glycyrrhrizae radix and the rest Atractylodis rhizoma and Coicis semen in water for 1.5 hr for 2 times, wherein the water addition amount is 8 and 6 times of the weight of the medicinal materials respectively; mixing decoctions, filtering, concentrating the filtrate to relative density of 1.25-1.30 (measured at 80 deg.C), drying the obtained soft extract under reduced pressure at vacuum degree of 0.08MPa or less and temperature of 70-80 deg.C to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain powder 3.
(6) Mixing powder 1, powder 2, and powder 3.
The Chinese medicinal composition can be made into pill, capsule or tablet, and can be used for reducing blood uric acid and preventing and treating gout.
Example 3
A traditional Chinese medicine composition for reducing blood uric acid comprises the following raw materials:
700g of selfheal, 40g of coptis root, 20g of bighead atractylodes rhizome, 20g of coix seed, 20g of achyranthes root and 20g of liquorice.
The preparation method of the traditional Chinese medicine composition for reducing blood uric acid comprises the following steps:
(1) Reflux-extracting Prunellae Spica with 80% ethanol solution for 2 times (2 hr each time), wherein the ethanol solution is 8 times of Prunellae Spica weight; extracting, filtering, and mixing filtrates to obtain Prunellae Spica ethanol extract.
(2) Recovering ethanol from the selfheal ethanol extracting solution until no ethanol smell exists, and concentrating until the mass ratio of the liquid medicine to the liquid medicine is 1; passing through D101 macroporous resin, washing with 4 times column volume of water, discarding water washing solution, eluting with 4 times column volume mass fraction 60% ethanol solution, collecting ethanol eluate, and recovering ethanol to obtain purified extract.
(3) Adding starch and beta-cyclodextrin into the purified extract, and spray drying to obtain powder 1 containing rosmarinic acid;
the air inlet temperature for drying is controlled to be 150-160 ℃, the air outlet temperature is controlled to be 80-90 ℃, the volume flow of inlet liquid is 15mL/min, and the pressure of atomizing airflow is 220Pa. The amount of starch was 10% of the purified extract mass and the amount of beta cyclodextrin was 10% of the purified extract mass.
(4) One third of the prescription weight of the rhizoma atractylodis macrocephalae and semen coicis is taken, crushed and sieved by a 80-mesh sieve to obtain powder 2.
(5) Decocting Coptidis rhizoma, achyranthis radix, glycyrrhrizae radix and the rest Atractylodis rhizoma and Coicis semen in water for 1.5 hr for 2 times, wherein the water amount is 8 and 6 times of the medicinal materials respectively; mixing decoctions, filtering, concentrating the filtrate to relative density of 1.25-1.30 (measured at 80 deg.C), drying the obtained soft extract under reduced pressure at vacuum degree of 0.08MPa or less and temperature of 70-80 deg.C to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain powder 3.
(6) Mixing powder 1, powder 2, and powder 3.
The Chinese medicinal composition can be made into pill, capsule or tablet, and can be used for reducing blood uric acid and preventing and treating gout.
Example 4
Experimental animals: kunming breed mice, SPF grade, male, supplied by Liaoning Biotechnology, inc., laboratory animal production license number: SCXK 2020-0001 (Liao) is provided.
License number for experimental animals: SYXK (Ji) 2022-0004. The feeding environment is a shielded environment, and food and water can be freely taken; the feed is provided by the Beijing department Australian cooperative feed Co., ltd, and the production license number is as follows: SCXK 2020-0004, batch number: 22013211.
the weight of a male Kunming mouse is 20-22 g, the male Kunming mouse is randomly divided into 7 groups, which are respectively a normal control group, a model control group, allopurinol (provided by combined Feitel pharmacy Co., ltd., batch number: 20180502) 5mg/kg group, simiao pills (rhizoma atractylodis, radix achyranthis bidentatae, golden cypress and semen coicis) 4g/kg group, an example 1 traditional Chinese medicine composition 2g/kg group and an example 1 traditional Chinese medicine composition 1g/kg group, and each group is 10 mice.
Performing intragastric administration on each group of 8 (am) and 00 (am) every day, preparing a liquid medicine by using 0.25% sodium carboxymethylcellulose (CMC) aqueous solution for each administration group, and performing intragastric administration, wherein the intragastric administration volume is 20mL/kg, and the continuous administration lasts for 6 days; gavage of the normal control group and the model control group was performed with an equivalent amount of 0.25% CMC solution.
After 1 hour of daily administration, 250mg/kg of oteracil potassium (supplied by Dalian biotechnology, inc., lot A0925A, prepared to a desired concentration with distilled water immediately before use, adjusted to pH7.5 with 1NKOH solution) was intraperitoneally administered to each of the model control group and each administration group, and the normal control group was intraperitoneally injected with an equal amount of physiological saline. Blood is taken from the retrobulbar venous plexus of the mouse 1h after the final potassium oxonate administration, the centrifugation is carried out for 10min at 3000rpm/min, serum is taken, and the serum uric acid level is determined by adopting a phosphotungstic acid reduction method according to the specification of a uric acid determination kit (provided by Nanjing institute of bioengineering, batch No. 20212225).
Statistical analysis data were in ± s, and a one-way anova was used for group comparisons.
The results are shown in Table 1. The blood uric acid level of the model control group animals is compared with that of the normal control group, and the difference has statistical significance (P < 0.01), which indicates that the model is successful. The positive drug allopurinol 5mg/kg has very obvious inhibition effect (P < 0.001) on the influence of the serum uric acid level of a hyperuricemia mouse model, the Simiao pill 4g/kg group has obvious inhibition effect (P < 0.05) on the influence of the serum uric acid level of the hyperuricemia mouse model, and the composition 4g/kg group and the composition 2g/kg group in the example 1 have obvious inhibition effect (P < 0.01) on the influence of the serum uric acid level of the hyperuricemia mouse model. 1g/kg has obvious inhibiting effect on the influence of the serum uric acid level of a hyperuricemia mouse model (P is less than 0.05).
TABLE 1
Comparing with a normal control group: : # p <0.001, compared to the model control group: * p <0.05, p <0.01, p <0.001.
The research adopts a potassium oxonate induced mouse hyperuricemia model, takes allopurinol as a positive control, and researches the influence of intragastric administration of the traditional Chinese medicine composition of different administration concentrations on the serum uric acid level of the hyperuricemia mouse model. The results show that the traditional Chinese medicine composition of the embodiment 1 has a certain effect of reducing the blood uric acid at 4g/kg and 2g/kg, the effect is obviously better than that of the Simiao pill group, and the inhibition of xanthine oxidase is one of the action targets.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the above-described embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A traditional Chinese medicine composition for reducing blood uric acid is characterized by comprising the following raw materials in parts by weight:
600-900 parts of selfheal, 10-50 parts of coptis chinensis, 10-50 parts of bighead atractylodes rhizome, 5-30 parts of coix seed, 5-30 parts of achyranthes root and 5-30 parts of liquorice.
2. The preparation method of the traditional Chinese medicine composition for reducing blood uric acid, according to claim 1, is characterized by comprising the following steps:
(1) Reflux-extracting Prunellae Spica with ethanol solution to obtain Prunellae Spica ethanol extractive solution;
(2) Recovering ethanol from Prunellae Spica ethanol extractive solution, concentrating, and purifying with macroporous resin;
(3) Spray drying the purified extract to obtain powder 1 containing rosmarinic acid;
(4) Pulverizing part of Atractylodis rhizoma and Coicis semen to obtain powder 2;
(5) Decocting Coptidis rhizoma, achyranthis radix, glycyrrhrizae radix and the rest Atractylodis rhizoma and Coicis semen in water, filtering the decoction, concentrating the filtrate into paste, drying, and pulverizing to obtain powder 3;
(6) Mixing powder 1, powder 2 and powder 3.
3. The preparation method of a Chinese medicinal composition for reducing blood uric acid as claimed in claim 2, wherein,
in the step (1), ethanol solution with the mass fraction of 40-95% is used for reflux extraction for 1-3 times, each time lasts for 0.5-2.5 hours, and the amount of the ethanol solution for reflux extraction for each time is 4-10 times of the weight of the selfheal.
4. The preparation method of a Chinese medicinal composition for reducing blood uric acid as claimed in claim 2, wherein,
in the step (2), recovering ethanol from the ethanol extract of the selfheal until no alcohol smell exists, and concentrating; passing through D101 macroporous resin, washing with water, discarding water washing solution, eluting with 20-80% ethanol solution, collecting ethanol eluate, and recovering ethanol to obtain purified extract.
5. The preparation method of a Chinese medicinal composition for reducing blood uric acid as claimed in claim 4, wherein,
the dosage of the water and the ethanol solution with the mass fraction of 20-80 percent are 4 times of the column volume.
6. The preparation method of a Chinese medicinal composition for reducing blood uric acid as claimed in claim 2, wherein,
in the step (3), starch and beta cyclodextrin are added into the purified extract, spray drying is carried out, the air inlet temperature for drying is 150-160 ℃, the air exhaust temperature is 80-90 ℃, the liquid inlet volume flow is 15mL/min, and the atomizing airflow pressure is 220Pa.
7. The preparation method of a Chinese medicinal composition for reducing blood uric acid as claimed in claim 6, wherein,
the amount of starch is 10% of the purified extract mass and the amount of beta-cyclodextrin is 10-15% of the purified extract mass.
8. The preparation method of a Chinese medicinal composition for reducing blood uric acid as claimed in claim 2, wherein,
in the step (4), the dosage of the largehead atractylodes rhizome and the coix seed is one third of the dosage of the prescription; pulverizing, and sieving with 80 mesh sieve.
9. The preparation method of a Chinese medicinal composition for reducing blood uric acid as claimed in claim 2, wherein,
in the step (5), water is added for 2 times, 1.5 hours each time, and the amount of the added water is 6-8 times of the weight of the medicinal materials; mixing decoctions, filtering, concentrating the filtrate to obtain soft extract with relative density of 1.25-1.30 at 80 deg.C, controlling vacuum degree at 0.08MPa or less and temperature at 70-80 deg.C, drying under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve.
10. Use of the Chinese medicinal composition of claim 1 or the Chinese medicinal composition prepared by the method of any one of claims 2 to 9 in the preparation of a medicament for reducing blood uric acid and preventing or treating gout.
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