CN1256097C - Yak-blood deproteined extract and its extraction method and use thereof - Google Patents
Yak-blood deproteined extract and its extraction method and use thereof Download PDFInfo
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- CN1256097C CN1256097C CNB021266298A CN02126629A CN1256097C CN 1256097 C CN1256097 C CN 1256097C CN B021266298 A CNB021266298 A CN B021266298A CN 02126629 A CN02126629 A CN 02126629A CN 1256097 C CN1256097 C CN 1256097C
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Abstract
The present invention relates to yak blood deproteinization extractive, a method and an application of the extractive. The yak blood deproteinization extractive is a physiological component whose molecular weight is less than 5000 or 6000 daltons. The purifying process comprises the steps that yak blood is taken under the aseptic condition, serum is separated, a Milipore ultrafilter is used for filtration, and ultrafiltrate is degermed, frozen and dried. The yak blood deproteinization extractive of the present invention can be used as medicine, health care food and food for treating diabetes and other complications and controlling various kinds of brain function incompetence such as senile dementia, craniocerebral trauma, brain stupor, etc. and various chronic altitude diseases.
Description
Technical field
The present invention relates to a kind of from yak deprotein extract and extracting method and application that (comprising the heifer in newborn six months), blood extracted.Belong to bio-pharmaceutical preparation, health food and foodstuff production method, reach application.
Background technology
People such as Parade are in nineteen sixty-eight, reported first calf (the refering in particular to cattle) blood deprotein extract in newborn six months, not only have ILA, can also irritation cell to the picked-up and the utilization of oxygen.Simultaneously, also have the scholar to notice the Sanguis Bovis seu Bubali deprotein extract of growing up, above biological activity is also arranged, but its effect far is inferior to the calf blood protein-removed extraction in newborn six months.Cause grow up Sanguis Bovis seu Bubali deprotein extract and the bioactive significant difference of new calves blood deprotein extract, mainly be since new calves in utero, stay in for a long time that the parent intrauterine of airtight anoxia causes, under such anaerobic environment, this para-insulin sample of tire Sanguis Bovis seu Bubali content of material increases, and helps intravital oxygen metabolism of tire cattle and energy metabolism.
Yak belongs to Bos, yak subgenus, yak kind in classification.According to the study, its physiological and biochemical property and cattle have bigger difference.China Qinghai-Tibet Platean is main cradle and the major production areas of yak, and the yak long term growth is multiplied in height above sea level 2500-5000 rice hypoxic plateau environment, and plateau severe cold, anoxybiotic weather are adapted to especially.In view of above life habit of yak and growing environment, the inventor is through years of researches, no matter find the yak that grows up, or new life's yak in 6 months, its blood all exists the extremely strong para-insulin material of biological activity, and the calf blood protein-removed extraction in the new life of people such as this material and Parade report six months, no matter be that molecular weight, composition and biological function are all very close.
Summary of the invention
One of purpose of the present invention provides a kind of highlands Yak-blood deproteined extract, this extract mainly is by promoting cell that the picked-up and the utilization (not relying on insulin) of glucose are increased absorption and the utilization of cell to oxygen, thereby ATP is generated to be increased, promote the cellular energy metabolism at cellular level, this extract can be applicable to medicine, health food and food, can be made into dosage forms such as injection, tablet, capsule and oral liquid.
Two of purpose of the present invention provides two kinds of preparation methoies of extracting the plateau yak blood deprotein extract.
Three of purpose of the present invention has provided a kind of Yak-blood deproteined extract and has been applied to range of application on medicine, health food and the food.
Above purpose of the present invention will and be described and further set forth by following detailed description.
A kind of Yak-blood deproteined extract that is provided among the present invention mainly is to obtain from plateau yak (comprising heifer in newborn six months) blood, according to the analysis of components of the inventor to Yak-blood deproteined extract, this kind extract molecular weight is less than 5000 or 6000 dalton, only contain the physiological composition, i.e. 60% inorganic substances are as Na
+, K
+, Cl
-, Ca
2+Deng the indispensable element of electrolyte and trace, and 40% organic substance is as the intermediate product of phosphoinositide oligosaccharide, aminoacid, low molecular polypeptide, nucleic acid derivative, glycolipid class and sugar and lipid metabolism etc.
The quality standard of the resulting Yak-blood deproteined extract of the present invention is: this extract is a sterile freeze-drying preparation, contains the 90.0-110.0% that the Sanguis Bovis seu Bubali derivant should be labelled amount; Character is the loose shape lyophilized powder of white or little yellow, and soluble in water, relative density is 1.021-1.025.
Yak-blood deproteined extract of the present invention can obtain by following two kinds of preparation methoies:
1、
(1), under aseptic condition, adopt yak blood, under 3 ℃～8 ℃ of temperature, 3000-5000rpm, centrifugal 10-40 minute, supernatant.
(2), get supernatant, utilize the membrane filtration clarification of 0.2-0.9 micron, clear liquor.
(3), clear liquor with Milipore ultrafilter molecular cut off less than the component below 6000 dalton (ultrasiltrated rate is the 10-1000ml/ branch, the wriggling pump pressure be≤0.1-0.5mpa).
(4), packing, lyophilizing.
2、
(1) adopt yak blood under aseptic condition, under 3 ℃～8 ℃ of temperature, 3000-5000rpm centrifugal 10-40 minute, must go up synovial fluid.
(2), get supernatant, add 70%-99% and double the serum volume of ethanol, constantly stirred static 60 minutes, make protein denaturation precipitation, through 10000-20000rpm4 ℃ of centrifugal 30-60 minute, stay supernatant then.
(3), utilize the rotary evaporation in vacuo instrument, 20 ℃-40 ℃, the decompression of 0.5-3 atmospheric pressure removes ethanol.
(4), supernatant with Milipore ultrafilter molecular cut off less than the component below 6000 dalton (ultrasiltrated rate is the 10-1000ml/ branch, the wriggling pump pressure be≤0.1-0.5mpa).
(5), packing, lyophilizing.
Product Yak-blood deproteined extract of the present invention is used in medicine, health food and food, is mainly used in: the treatment of (1) type ii diabetes; (2) treatment of diabetic polyneuropathy; (3) treatment of the brain insufficiency that causes of alzheimer disease, craniocerebral trauma, apoplexy and various coma; (4) the various acute and chronic altitude sickness treatment that is acute high altitude reaction, plateau pneumochysis, high altitude heart disease and high altitude erythrocytosis.When product Yak-blood deproteined extract of the present invention is applied to medicine, health food and food, can be made into dosage forms such as injection, tablet, soft capsule, hard capsule and oral liquid.
The specific embodiment
Embodiment 1:
Yak-blood deproteined extract of the present invention can obtain by following preparation method:
(1), under aseptic condition, adopt yak blood, under 4 ℃ of temperature, 4000rpm, centrifugal 20 minutes, supernatant.
(2), get supernatant, utilize 0.3 micron membrane filtration clarification, clear liquor.
(3), clear liquor with Milipore ultrafilter molecular cut off less than the component below 6000 dalton (ultrasiltrated rate is the 200ml/ branch, the wriggling pump pressure be≤0.2mpa).
(4), packing, lyophilizing.
Embodiment 2:
Yak-blood deproteined extract of the present invention also can obtain by following preparation method:
(1) adopt yak blood under aseptic condition, under 4 ℃ of temperature, 4000rpm centrifugal 20 minutes, gets supernatant.
(2), get supernatant, add 95% and double the serum volume of ethanol, constantly stirred static 60 minutes, make protein denaturation precipitation, then through 20000rpm4 ℃ centrifugal 30 minutes, stay supernatant.
(3), utilize the rotary evaporation in vacuo instrument, 40 ℃, the decompression of 1 atmospheric pressure removes ethanol.
(4), supernatant with Milipore ultrafilter molecular cut off less than the component below 6000 dalton (ultrasiltrated rate is the 200ml/ branch, the wriggling pump pressure be≤0.2mpa).
(5), packing, lyophilizing.
Embodiment 3:
Yak-blood deproteined extract of the present invention is to the influence of white mice acute anoxia endurance: treating excess syndrome is tested animal Kunming kind white mice, male, 10 ages in week, divide two groups at random by body weight, 30 every group, preceding 1 hour of anoxia, medicine group and matched group per os respectively give Yak-blood deproteined extract 3mg/kg, with equivalent starch, application of reduced pressure anoxia method, the anoxia tolerance of observing white mice.Anoxia height ten thousand metres, the rate of climb 15 meter per seconds are observed animal dead rate in 1 hour.The result: the survival rate of the group of taking medicine white mice is 50%, and the matched group survival rate is 18%.Hence one can see that, and Yak-blood deproteined extract can significantly improve the anoxia tolerance of animal.
Embodiment 4:
Regulating action to noninsulindependent diabetes white mice blood glucose: get the noninsulindependent diabetes white mice, male, 10 ages in week, be divided into two groups at random, 15 every group, medicine group and matched group per os respectively give Yak-blood deproteined extract 3mg/kg, and starch, once-a-day, 5 days weeks, 4 weeks of administration altogether.4 weeks got blood before the administration and after the administration, measure the concentration of glucose in the blood, reach the blood of getting in the 4th week of administration before the administration and all after jejunitas 12 hours, carry out by the tail vein.White mice blood glucose is starkly lower than the preceding level (seeing the following form) of administration after 4 weeks of administration as a result
| Grouping | Blood glucose value (mmol/L) | |
| Before the administration | After 4 weeks of administration | |
| Matched group administration group | 17.86±1.22 17.09±1.08 | 16.94±1.38 9.43±0.89 * |
*＝P＜0.01
Embodiment 5:
To the effect of diabetic polyneuropathy:
Observe by 100 routine diabetic polyneuropathy patients having been carried out the double blinding of Yak-blood deproteined extract matched group, the nerve conduction velocity of finding treatment group patient continues to increase, when treating for 8 weeks,, be increased to 41.02 meter per seconds from 37.09 initial meter per seconds; Pain sensation degree drops to 43.38 from 61.20.In addition, patient's the vibration sense and the sense of heat all make moderate progress.
Embodiment 6:
Therapeutical effect to ischemic encephalopathy:
By 208 routine ischemic encephalopathys (hemiplegia) being used the observation of Yak-blood deproteined extract treatment, find that the patient uses and reach 82.08% with part rehabilitation rate fully behind the Yak-blood deproteined extract, illustrate said preparation treat aspect the hemiplegia evident in efficacy.
Claims (7)
1, a kind of Yak-blood deproteined extract is characterized in that molecular weight less than 5000-6000 dalton, and described extract contains 60% inorganic substances, and described inorganic substances are Na
+, K
+, Cl
-, Ca
2+The indispensable element of electrolyte and trace, and 30% organic substance, described organic substance is the intermediate product of phosphoinositide oligosaccharide, aminoacid, low molecular polypeptide, nucleic acid derivative, glycolipid class and sugar and lipid metabolism.
2, Yak-blood deproteined extract as claimed in claim 1, yak blood wherein mainly refer to move in the adult yak of highlands and newborn six months heifer blood.
3, Yak-blood deproteined extract as claimed in claim 1, molecular weight wherein are meant less than 5000 dalton or less than 6000 daltonian Yak-blood deproteined extracts.
4, the production method of Yak-blood deproteined extract as claimed in claim 1 is that the yak blood with the Qinghai-Tibet Platean is a raw material, it is characterized in that:
(1), under aseptic condition, adopt yak blood, under 3 ℃～8 ℃ of temperature, 3000-5000rpm, centrifugal 10-40 minute, supernatant;
(2), get supernatant, utilize the membrane filtration clarification of 0.2-0.9 micron, clear liquor;
(3), clear liquor uses Milipore ultrafilter molecular cut off less than the component below 6000 dalton;
(4), packing, lyophilizing.
5, the production method of Yak-blood deproteined extract as claimed in claim 1 also can be:
(1), under aseptic condition, adopt yak blood, under 3 ℃～8 ℃ of temperature, 3000-5000rpm, centrifugal 10-40 minute, supernatant;
(2), get supernatant, add 70%-99% and double the serum volume of ethanol, constantly stirred static 60 minutes, make protein denaturation precipitation, through 10000-20000rpm4 ℃ of centrifugal 30-60 minute, stay supernatant then;
(3), utilize the rotary evaporation in vacuo instrument, 20 ℃-40 ℃, the decompression of 0.5-3 atmospheric pressure removes ethanol;
(4), supernatant uses Milipore ultrafilter molecular cut off less than the component below 6000 dalton;
(5), packing, lyophilizing.
6, Yak-blood deproteined extract as claimed in claim 1 is in medicine, health food and Application in Food, and described medicine, health food and food are used for: the treatment of (1) type ii diabetes; (2) treatment of diabetic polyneuropathy; (3) treatment of the brain insufficiency that causes of alzheimer disease, craniocerebral trauma, apoplexy and various coma; (4) the various acute and chronic altitude sickness treatment that is acute high altitude reaction, plateau pneumochysis, high altitude heart disease and high altitude erythrocytosis.
7, Yak-blood deproteined extract is characterized in that making dosage forms such as injection, tablet, soft capsule, hard capsule and oral liquid in medicine, health food and Application in Food according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021266298A CN1256097C (en) | 2002-07-25 | 2002-07-25 | Yak-blood deproteined extract and its extraction method and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021266298A CN1256097C (en) | 2002-07-25 | 2002-07-25 | Yak-blood deproteined extract and its extraction method and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1470249A CN1470249A (en) | 2004-01-28 |
| CN1256097C true CN1256097C (en) | 2006-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021266298A Expired - Fee Related CN1256097C (en) | 2002-07-25 | 2002-07-25 | Yak-blood deproteined extract and its extraction method and use thereof |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355427C (en) * | 2005-04-20 | 2007-12-19 | 沈阳斯佳科技发展有限公司 | Method for extracting substance with cell respiration stimulating activity from pig blood |
| CN101947242B (en) * | 2010-09-26 | 2012-05-23 | 武汉华龙生物制药有限公司 | Preparation method of deproteinized calf blood extract |
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2002
- 2002-07-25 CN CNB021266298A patent/CN1256097C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1470249A (en) | 2004-01-28 |
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