CN1248623A - Bacillus culture medium and fermentation process thereof - Google Patents
Bacillus culture medium and fermentation process thereof Download PDFInfo
- Publication number
- CN1248623A CN1248623A CN99121941A CN99121941A CN1248623A CN 1248623 A CN1248623 A CN 1248623A CN 99121941 A CN99121941 A CN 99121941A CN 99121941 A CN99121941 A CN 99121941A CN 1248623 A CN1248623 A CN 1248623A
- Authority
- CN
- China
- Prior art keywords
- filter mud
- bacillus
- culture medium
- fermentation process
- substratum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 21
- 238000000855 fermentation Methods 0.000 title claims abstract description 13
- 230000004151 fermentation Effects 0.000 title claims abstract description 13
- 239000001963 growth medium Substances 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000004615 ingredient Substances 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 5
- 244000005700 microbiome Species 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 description 7
- 239000000203 mixture Substances 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 241000193752 Bacillus circulans Species 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 2
- 241000193764 Brevibacillus brevis Species 0.000 description 2
- 241000222178 Candida tropicalis Species 0.000 description 2
- 241000194105 Paenibacillus polymyxa Species 0.000 description 2
- 241000589776 Pseudomonas putida Species 0.000 description 2
- 241001454747 Streptomyces aureus Species 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The present invention relates to a culture medium for bacillus and its fermentation method. Said invention selects the extract produced by using sulphitation process to produce cane sugar-filter mud as culture medium for bacillus and other some microorganisms. Said filter mud contains a lot of organic substances and elements of N, P, K, Ca and Mg, and can promote the strong growth of microorganisms of bacillus,etc. and can prevent disintegration, and at the same time, it solves the problem of "three wastes" of sugar mill, and is favourable for environmental protection.
Description
The present invention relates to the microbial engineering field, particularly a kind of bacillus culture medium and fermentation process thereof.
Existing general microorganism comprises that the composition commonly used of the third stage substratum of genus bacillus is: wheat bran, beautiful chaff, milk powder, soyflour, meat meal tankage, fishbone powder, sugar etc. add suitable quantity of water and mix into substratum thoroughly.After the inoculation, under certain temperature, humidity and pH value condition, through a few days fermentation, receive the finished product thalline.These finished product thalline and carrier are mixed thoroughly and are promptly become microniological proudcts.Carrier can be peat soil, wooden chaff etc.Substratum and carrier are chosen respectively.Produce the extractum of sucrose with sulfurous method---filter mud is not seen so far as genus bacillus third stage substratum and was reported that this filter mud had both had the nutrient of enriching, and can be used as microbe carrier again.
The purpose of this invention is to provide a kind of suitable genus bacillus substratum and the fermentation process thereof of growth fast.
The substratum of a kind of suitable genus bacillus growth of the present invention is extractum a---filter mud of producing sucrose with sulfurous method, and its major ingredient is: organism, N, P
2O
5, K
2O, CaO, MgO, the major ingredient of this filter mud and content mean value are (weight percent):
Organism: 58.66 N:1.19
P
2O
5：?1.24 K
2O：?0.52
CaO： 5.59 MgO： 1.07
This substratum composition contains bioelement and the organic content that promotes the genus bacillus growth, and its composition and content are more reasonable.Be particularly suitable as genus bacillus third stage substratum.
It is as follows that the present invention cultivates the fermentation process of genus bacillus: filter mud is inoculated when temperature is 20 ℃-50 ℃, and inoculum size is 0.01-1%, inoculation back layering stack retting; During fermentation vaccinated filter mud temperature rises to 60 ℃-65 ℃ gradually, pH value 6.8-7.2; Through 7-12 days fermenting-ripenings, become the base-material of biological organic fertilizer.
Contain large amount of organic and elements such as abundant and N, P, K, Ca, Mg in the substratum filter mud of the present invention, it itself is genus bacillus third stage substratum, it also is its carrier, after the inoculation, genus bacillus ramp under nutritious and good environment condition, behind 7-12 days fermenting-ripenings, viable count reaches 1,000,000,000-1,000 hundred million/gram.In the substratum effect of contained abundant various elements be to regulate, replenish in the genus bacillus growth can not get in the organism or few required element, make the genus bacillus growth strong, prevent to degrade.
Produce the extractum of sucrose with sulfitation process craft---filter mud is the refuse that discharge in sugar refinery; but it contains organic material up to more than 50%; elements such as N, P, K, Ca, Mg are no less than 2%; it is the low and substratum of nutritive ingredient is high suitable cultivation genus bacillus and some other microorganism of a kind of cost that finds easily; refuse is become treasured; " three wastes " problem of solution helps producing, and helps environment protection.
Embodiment one:
With sulfurous method produce sucrose extractum---filter mud is as the bacillus megaterium substratum, and the major ingredient of this filter mud and content mean value are (weight percent):
Organism: 58.66 N:1.19
P
2O
5：1.24 K
2O：?0.52
CaO： 5.59 MgO： 1.07
Filter mud pH value 6.0.
It is as follows that the present invention is used for the bacillus megaterium fermentation process: the filter mud temperature is inoculated in the time of 30 ℃, and inoculum size is 0.1%, inoculation back layering stack retting; Vaccinated filter mud temperature rises to 63 ℃ gradually between yeast phase, pH value 7.0.Fermenting, the filter mud surface begins to grow mycelia after three days, and its transverse section also begins to grow mycelia, through 9 days fermenting-ripenings, becomes biological organic base-material, and this base-material contains viable count and reaches 1,000,000,000-1,000 hundred million/gram.
Embodiment two:
With sulfurous method produce sucrose extractum---filter mud is as the substratum of bacillus brevis, Bacillus licheniformis, bacillus polymyxa and streptomyces aureus, and the major ingredient of this filter mud and content mean value are identical with example one.Filter mud pH value 6.0.
It is as follows that the present invention is used for the fermentation process of bacillus brevis, Bacillus licheniformis, bacillus polymyxa and streptomyces aureus: the filter mud temperature is inoculated in the time of 50 ℃, and inoculum size is 0.01%, inoculation back layering stack retting; Vaccinated filter mud temperature rises to 65 ℃ gradually between yeast phase, pH value 7.2.Fermenting, the filter mud surface begins to grow mycelia after three days, and its transverse section also begins to grow mycelia, through 7 days fermenting-ripenings, becomes biological organic base-material, and this base-material contains viable count and reaches 1,000,000,000-1,000 hundred million/gram.
Embodiment three:
With sulfurous method produce sucrose extractum---filter mud is as the substratum of subtilis, bacillus cereus, Bacillus circulans and candida tropicalis, pseudomonas putida, and the major ingredient of this filter mud and content mean value are identical with embodiment one.Filter mud pH value 6.0.
It is as follows that the present invention is used for the fermentation process of subtilis, bacillus cereus, Bacillus circulans and candida tropicalis, pseudomonas putida: the filter mud temperature is inoculated in the time of 20 ℃, and inoculum size is 1%, inoculation back layering stack retting; Vaccinated filter mud temperature rises to 60 ℃ gradually between yeast phase, pH value 6.8.Fermenting, the filter mud surface begins to grow mycelia after three days, and its transverse section also begins to grow mycelia, through 12 days fermenting-ripenings, becomes the base-material of biological organic fertilizer, and this base-material contains viable count and reaches 1,000,000,000-1,000 hundred million/gram.
Claims (3)
1, a kind of bacillus culture medium is characterized in that: described substratum is for producing extractum---the filter mud of sucrose with sulfurous method, its major ingredient is: organism, N, P
2O
5, K
2O, CaO, MgO.
2, bacillus culture medium according to claim 1 is characterized in that: the major ingredient of described filter mud and content mean value thereof are (weight percent):
Organism: 58.66 N:1.19
P
2O
5： 1.24 K
2O：?0.52
CaO： 5.59 MgO： 1.07
3, the fermentation process of a kind of genus bacillus is characterized in that: filter mud is inoculated when temperature is 20 ℃-50 ℃, and inoculum size is 0.01-1%, inoculation back layering stack retting; During fermentation vaccinated filter mud temperature rises to 60 ℃-65 ℃ gradually, pH value 6.8-7.2; Through 7-12 days fermenting-ripenings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99121941A CN1110548C (en) | 1999-10-18 | 1999-10-18 | Bacillus culture medium and fermentation process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99121941A CN1110548C (en) | 1999-10-18 | 1999-10-18 | Bacillus culture medium and fermentation process thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1248623A true CN1248623A (en) | 2000-03-29 |
| CN1110548C CN1110548C (en) | 2003-06-04 |
Family
ID=5282204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99121941A Expired - Lifetime CN1110548C (en) | 1999-10-18 | 1999-10-18 | Bacillus culture medium and fermentation process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1110548C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104120098A (en) * | 2014-06-25 | 2014-10-29 | 广西师范大学 | High-temperature-resistant polyhydroxybutyrate-generating bacterium |
| CN105084948A (en) * | 2015-09-28 | 2015-11-25 | 广西壮族自治区农业科学院甘蔗研究所 | Organic fertilizer with high humic acid content and preparation method thereof |
| CN105622176A (en) * | 2014-10-26 | 2016-06-01 | 广西来宾市天仁生物有机肥有限责任公司 | Method for resource production of biological organic fertilizer from sugar refinery filtration mud |
| CN105766813A (en) * | 2016-04-13 | 2016-07-20 | 江苏省中国科学院植物研究所 | Method for gathering and cultivating aphelenchoidessp |
-
1999
- 1999-10-18 CN CN99121941A patent/CN1110548C/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104120098A (en) * | 2014-06-25 | 2014-10-29 | 广西师范大学 | High-temperature-resistant polyhydroxybutyrate-generating bacterium |
| CN105622176A (en) * | 2014-10-26 | 2016-06-01 | 广西来宾市天仁生物有机肥有限责任公司 | Method for resource production of biological organic fertilizer from sugar refinery filtration mud |
| CN105084948A (en) * | 2015-09-28 | 2015-11-25 | 广西壮族自治区农业科学院甘蔗研究所 | Organic fertilizer with high humic acid content and preparation method thereof |
| CN105084948B (en) * | 2015-09-28 | 2018-02-06 | 广西壮族自治区农业科学院甘蔗研究所 | High organic fertilizer of a kind of content of humic acid and preparation method thereof |
| CN105766813A (en) * | 2016-04-13 | 2016-07-20 | 江苏省中国科学院植物研究所 | Method for gathering and cultivating aphelenchoidessp |
| CN105766813B (en) * | 2016-04-13 | 2018-10-09 | 江苏省中国科学院植物研究所 | A kind of method that enrichment culture slides sword category Fungal-feeding nematode |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1110548C (en) | 2003-06-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Applicant after: Gaoming Jinkuizi Plant Nutrient Co. Ltd. Applicant before: Lubao Plant Nutritious Products Co., Ltd., Gaoming City |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: LUBAO PLANT NUTRITIOUS PRODUCTS CO., LTD., GAOMING CITY TO: GAOMING JINKUIZI PLANT NUTRITION CO., LTD. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: FUSHAN JINKUIZI VEGETABLE NUTRITION CO., LTD. Free format text: FORMER NAME OR ADDRESS: GAOMING JINKUIZI PLANT NUTRITION CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 528518, No. two, No. 383, Ming Cheng town, Gaoming District, Guangdong, Foshan Patentee after: Foshan Jinkuizi Plant Nutriment Co., Ltd. Address before: 528518, Ming Town, Guangdong Province, white tube development zone Patentee before: Gaoming Jinkuizi Plant Nutrient Co. Ltd. |
|
| ASS | Succession or assignment of patent right |
Owner name: JINKUIZI ENVIRONMENTAL PROTECTION BIOLOGY TECHNOLO Free format text: FORMER OWNER: FOSHAN JINKUIZI PLATE NUTRITION CO., LTD. Effective date: 20100528 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 528518 NO.383, CHENGER ROAD, MINGCHENG TOWN, GAOMING DISTRICT, FOSHAN CITY, GUANGDONG PROVINCE TO: ROOM 1007-9, FLOOR 10, WEIDA BUSINESS BUILDING, 1A/1J, TONGCAI STREET, MONGKOK, KOWLOON, HONGKONG, CHINA |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20100528 Address after: Chinese Hongkong Kowloon Tong Choi Street 1A/1J Weida commercial building 10 floor 1007-9 room Patentee after: Jinkuizi Environmental Biotechnology (International) Co., Ltd. Address before: 528518, No. two, No. 383, Ming Cheng town, Gaoming District, Guangdong, Foshan Patentee before: Foshan Jinkuizi Plant Nutriment Co., Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20030604 |
|
| CX01 | Expiry of patent term |