CN1248461A - Health care oral liquid for old people and preparation process thereof - Google Patents

Abstract

The present invention relates to a senile health-care oral liquor and its preparation method. Its basic composition includes the Chinese medicinal materials of scutellaria root, earthworm, epimedium, bee glue, sea-buckthorn and licorice, etc.. Said invented oral liquor is simple in preparation process, and possesses the functions of inhibiting lipid oxygenase, antioxidation, removing peroxidated free radical in vive, protecting vitamin c, resisting senility, raising immunity of human body, inhibiting allergic reaction, raising metabolism level of nucleic acid, resisting cancer, mutation and virus, relieving inflammation and resisting pathogenic microorganism, so that it has hight medical value for curing and preventing senile diseases of hypertension, angiocardiopathy, diabetes, bronchitis, pneumonia, gastric ulcer, tumor and various pathogenic microbial infections.

Description

A kind of old with health care oral liquid and preparation method thereof

The present invention relates to a kind of is that old age of main effective ingredient is with health care oral liquid and preparation method thereof with natural plant extracts, can be used for prevention and treat old common frdquently encountered disease, old common diseases such as hypertension, cardiovascular disease, diabetes, bronchitis, pneumonia, gastric ulcer and hepatic injury, tumor and multiple cause pathogeny imcrobe infection are all had positive prevention and therapeutical effect.

World population is just in ageing, and the speed of aging population is 2 times of total population growth rate, and the speed that the man at an advanced age increases more than 80 years old is faster, is 6 times of total population growth rate.China present more than 60 years old old people be 1.1 hundred million, to mid-21st Century, will reach 4.1 hundred million, the developed country such as the U.S. are no exception, the fact of the former unforeseeable aging of population occurs.The beginning of this century, 4% American has risen to 13% now over 65 years old; Present life expectancy exceedes 76 years old, can reach 83 years old to the year two thousand fifty.WHO estimates that in 20 years from now on, the situation of developing country's aged tendency of population will particularly sternly be completed, and to the year two thousand twenty, surpasses in 60 years old the population more than hundred million surplus in the of global 10, and 2/3 lives in these developing countries.The United Nations's regulation was world old man year in 1999, and its theme is still " healthy aging ".

Be accompanied by the aging of population, the sickness rate of Senile disease also will increase, China can not make an exception, it brings white elephant and misery for individual, family and society, and in the existing health product, the health product of effective anti-ageing diseases prevention of considering old pathology physiological characteristics specially are actually rare, can not satisfy social needs.

Can't the research of antiaging agent assert with good groundsly that so far any medicine has anti-ageing effect to the people really owing to be subjected to the restriction of following two principal elements?The firstth, old and feeble mechanism problem.How aging takes place actually, goes back the theory that neither one is generally acknowledged, thereby makes the design of antiaging agent lose prerequisite and foundation; The secondth, defying age index problem.How just to calculate anti-aging effects arranged? generally believe it is to want to improve maximum life span at present; But can improve the medicine of animal maximum life span, can not directly spread to the mankind.Present some " antiaging agents " of releasing, majority is with reference to existing certain old and feeble theory, and along with the growth and decline situation that increases some materials in the human body in age (hormone, enzyme and free radical etc.), designs.Thereby being difficult to exactly any medicine has anti-aging effects.The China deputy chairman Lv Baozhang researcher of Gerontological Society briefly is divided into hormone, antioxidant, nucleic acid preparation three classes with relevant antiaging agent, and the spy is described below.

Amcinonide: the hormones antiaging agent that occurs with medicine or health food appearance on market is many at present, for example melatonin, growth hormone, dehydroepiandros-sterone (DHEA), gonad preparation and adrenocortical hormone etc.

Melatonin is domestic to have the people according to its English name Melatonin,, transliteration is " * * peaceful " " * * holder is peaceful " or the like.It is the excretory a kind of hormone of pinus in the brain, and its main effect is a circadian rhythm of regulating human body, and this hormone also has regulating action to other endocrine gland in the body in addition.Discovering in recent years, melatonin is except that keeping normal sleep rule, also have the effect of 1. removing free radical: it can see through all physiologic barriers, arrive each internal organs of health, brain particularly, and can be deep in the cell, through nucleus, it is removed non-other antioxidants of ability of free radical and can compare.2. raise immunity: the thymus that produces the T cell is had facilitation, thereby make T cytosis in the body.Because the T cell is relevant with some bioactie agents generations in the body, is the dominant role of cellular immunization, thereby its this effect, quite induces one to pay attention to.Clinical and zoopery proves that this hormonal readiness reduces in cancer patient's blood, take this hormone after, the effect of strengthening anticancer drug effect is arranged, and can prolong life.3. anti-aging effects: the pinus of young animal is transplanted in the aged animal body, can be made it young; Otherwise, the pinus of aged animal is transplanted to young animal, can quicken its aging and even dead.But, transplant the shown effect of pinus, being not equal to is the effect of melatonin, because except that this hormone, tens kinds of other biological active substances can also be synthesized and secrete to pinus.And because melatonin under normal circumstances except that regulating circadian rhythm, also has regulating action to other endocrine gland, its secretion also is subjected to the adjusting of adrenoreceptor on the pinealocyte; Therefore, it is still waiting to observe to the long-range influence of human body, can't take for a long time as a kind of antiaging agent at present at least.Because pinus is as an endocrine organ, it depends on the demand of periphery to this hormone to the secretion of melatonin, the many just many secretions of demand, many supplies, the just few less secretion of demand.If original existing enough melatonins in the body, introduce external " product " again blindly, certainly will make this hormone excessive, this situation can have influence on pinus in time, the function that pinus is produced this hormone will fade away, therefore pinus itself also will be and atrophy, and Here it is moves back with advancing to give up.Consequently: be for defying age originally, and but quickened pineal decline, body is brought bad influence.Have report to show that this hormone has the tumor stimulation still more, this is worth having enhanced your vigilance very just.

It is one of metabolite of testosterone for a dehydroepiandros-sterone (DHEA), once is sensational " defying age medicine ".One of the U.S. in 1988 studies show that, DHEA has the middle aged man of minimizing to suffer from cardiovascular diseases's effect; After the people is arranged through clinical observation again, find that it has the effect that improves sleep, emotion and bear the life stress ability to most patient; In " DHEA is with old and feeble " seminar that June nineteen ninety-five, USA New York academy of science held, most of participants think that DHEA has anticancer and effect life lengthening.This conclusion once formed the tide of one panic buying DHEA product through the propagation of each side.But the research of DHEA and finder Bo Li but hold very careful attitude to this, and he thinks that DHEA can improve the quality of living, and alleviate because of old and feeble fatigue that produces and weakness etc., but can't increase man's lifespan.His lay special stress on, people's aging is caused by multiple factor, only depends on a kind of medicine can not reach antidotal purpose.Still more, the someone reports recently, influences the synthetic and not variation of metabolic adjusting of human body DHEA in ageing process as not being subjected to major disease.The scientist of Germany finds that by the comparative study to 77 59～81 years old male DHEA there is no improvement to the state (as cardiovascular system) of human body.Obviously, the anti-aging effects to DHEA does not still have final conclusion.

Second class is an antioxidant: the foundation of this class drug development is the free radical theory that the aging that is subjected to extensively paying attention at present takes place, and product has appeared on the market mainly contains vitamin E, C and beta-carotene.

Why the vitamin E vitamin E has been considered to anti-aging effects, mainly is to come from the influence that it generates lipofuscin.Lipofuscin is the polyunsaturated fatty acid of lipid in the cell, under the effect of free radical, and the lipid peroxide of generation.According to the study, in the mice spinal nerve section cell in one week of birth, the amount of lipofuscin increased to 1100 when being 0,8 month, can reach nearly 2500 by 18 months, promptly increased along with increasing age; In the myocardial cell of mice, if the lipofuscin amount during with 2.5 monthly ages is a benchmark, increased by 2.5 times during then by 18 months, increased by 7 times during by 28 months, also be with increasing age; And when adding accounted for the vitamin E of food weight 0.25% in food, then the lipofuscin in each age group mouse cardiac muscle cell but reduced to some extent, had reduced nearly 40% as 2.5 monthly age persons.Average life can be increased to contain the food nutrition purposes, especially for feeding animals of vitamin E, to prove, but maximum life span can not be improved.Therefore, also there is not ample evidence explanation vitamin E that anti-aging effects is arranged at present.But what have studies confirm that, vitamin E benefits for improving old people's functions of immune system.For example " JAMA " report, 88 over-65s old peoples are divided into 4 groups, take 60 milligrams of vitamin Es, 200 milligrams, 800 milligrams and placebo respectively every day, after 4 months, the old people that 200 milligrams of vitamin Es are taken in discovery every day, the raising 65% of the function ratio clothes placebo group of T cell, immune system has improved 6 times to the respond of Hepatitis B virus vaccine; Take 800 milligrams of persons every day, identical with the effect of taking 200 milligrams; And take 60 milligrams poor effect.Thereby think, only depend on the vitamin E that from food, obtains every day, be not enough to improve immunocompetence, should under physician guidance, strengthen consumption.Someone advises, it and vitamin C are taken simultaneously, can reinforced effects.

The vitamin C vitamin C also has stronger antioxidation.But it enters in the cell not too easily, and large dose oral administration can cause the damage of DNA in the cell.Recently, a research group of Japan is incorporated into phosphate in the vitamin C molecule, and changes its part-structure, forms " vitamin C precursor ", and the latter enters in the cell easily, and discharges vitamin C; The experiment of carrying out with human body cell shows that the speed that it can make telomere shorten reduces by 27%, and strengthens the activity of telomerase; This processing can make the life-span of cell prolong 50%, and this also conforms to the old and feeble telomere theory that takes place.It seems that this may be a kind of rising antiaging agent.

The anti-aging effects of beta-carotene beta-carotene may be because contain more two keys in its molecular structure, and is oxidized easily, is considered to have antioxidation; What also have really studies have shown that, it is active high to take in the animal body of carotene SOD, and lipofuscin content is low etc. in the cell.Known now, beta-carotene is to diseases such as angiocardiopathy preventing, cancer and senile cataracts, and the raising immunologic function, and certain effect is all arranged; In this sense, also has anti-aging effects.

Be nucleic acid preparation at last, the intravital nucleic acid of people has two big classes, i.e. DNA (deoxyribonucleic acid) (DNA) and ribonucleic acid (RNA), and this is 20 for many years molecular biological growing points.At first illustrate the fertile gloomy and Ke Like of nucleic acid structure and genetic code the beginning of the fifties, therefore obtain Nobel Prize.We can say, a lot of major progresses of modern biology, medical science, such as the vaccine of the discovery of HIV (human immunodeficiency virus), viral hepatitis and gene therapy etc., relevant with this achievement invariably.Inject the mix preparation of these two kinds of nucleic acid to animal, can improve its maximum life span significantly.The experiment that Chinese scholar is carried out shows that nucleic acid preparation can obviously increase old people's oxidation resistance, reduces lipid peroxide content.Abroad the someone has observed the effect after thousands of people inject nucleic acid preparation, finds that it can make people's skin seem young, and physical strength reinforcing makes moderate progress to the symptom of coronary heart disease, diabetes and hyperlipemia etc.The achievement in research to the nucleic acid anti-disease, anti-aging has been summed up by Japan scholar Song Yongzheng department, has write " miracle of Source of life---nucleic acid " book, the effect of nucleic acid is praised highly add fully.It seems that the anti-aging effects of nucleic acid preparation is very significant, these effects have any relation with old and feeble gene program control theory that takes place and telomere theory, also are worth further inquiring into.

In a word, above introduction explanation does not also have any medicine to have comprehensive defying age effect so far, and people should not post with too high expectation this yet.And studies show that contain " flavonoid " (oak skin alcohol) and lycopene in some food, their antioxidation and antitumaous effect are better than vitamin E and C, be a kind of extremely promising anti-ageing diseases prevention class medicine.The effective ingredient of seeking anti-ageing diseases prevention from natural plants has been trend of the times, and natural botanical extraction liquid has become the effective way that we prevented and treated old common disease.Therefore; this invention has the old physiological function of protection from " flavonoid "; suppress lipoxygenase; antioxidation; remove human body endoperoxides free radical; the protection vitamin C is exempted from oxidized destruction; strengthen body immunity and suppress allergy; improve the nucleic acid metabolism level; anti-promoting; anti-cause prominent; antiviral; antiinflammatory; set out in aspects such as anti-multiple source of disease microorganism; adopt unique standard and method; through a large amount of plants are screened for a long time; identify; from natural plant extracts, sum up and use the health oral formula of liquid a kind of old age; the limited production promising result that obtains on probation at home and abroad; after discussing, intend large-scale production with the insider; be the protection inventor's economic interests, special to applying for a patent with health oral formula of liquid and preparation method thereof this old age.

The object of the present invention is to provide a kind of having of from natural plants, extracting to suppress lipoxygenase, antioxidation, removing human body endoperoxides free radical; the protection vitamin C exempt from oxidized destruction, defying age, enhancing body immunity and suppress allergy, improve the nucleic acid metabolism level, anti-promoting, anti-causing use health care oral liquid bioactive old age such as prominent, antiviral, antiinflammatory, anti-multiple source of disease microorganism; be used to protect old physiological function, prevention and treatment old common disease, its basic proportioning is as follows:

(1) Radix Scutellariae is 10 kilograms

(2) Pheretima (Lumbricus) is 10 kilograms

(3) Herba Epimedii is 10 kilograms

(4) Radix Glycyrrhizae is 10 kilograms

(5) Fructus Hippophae is 10 kilograms

(6) propolis liquid is 30 kilograms

(7) ethanol is 60 liters;

(8) sodium benzoate 100-300g;

(9) distilled water adds to 100 liters;

Its preparation method is:

Get earlier (1)-after (5) number raw material is cleaned and pulverized, with water retting 30 minutes, adding 5 times of water gagings earlier, decocted 1 hour, totally 2 times, collecting decoction, cross 100 mesh sieves, filtrate has descended concentrating under reduced pressure to become 30 liters in 70 degree, puts cold, under agitation, slowly add ethanol, make the alcohol amount of containing reach 60% (volume fraction), leave standstill cold preservation 48 hours, and filtered, reclaim ethanol, be concentrated into 1: 1, medicinal liquid cold preservation 24 hours is filtered, filtrate under agitation adds strong aqua ammonia and adjusts pH value to 7.0～8.0, and cold preservation 24 hours is filtered, the deammoniation of filtrate heated and stirred, medicinal liquid remain on 100～102 the degree, 2～2.5 hours, to the medicinal liquid pH value be 5.8, cold preservation is spent the night, and filters standby.

Get above-mentioned medicinal liquid, adjust pH to 5～7, add 30 liters of sodium benzoate 100 grams and propolis liquid with the 100g/L sodium carbonate liquor, stir, filter, measure content by the G3 sintered glass funnel, add water to 100L again, stir evenly fill, plug plug, roll easy-open lid, sterilization, through quality examination, labeling promptly.

Effect is discussed:

As previously mentioned, modern medicine study shows, contains " flavonoid " (oak skin alcohol) and lycopene in some food, and their antioxidation and antitumaous effect are better than other drugs such as vitamin E and C.Also more economical, safety and effective.They can directly catch oxygen-derived free radicals and hydroxy radical, suppress brain MAO-B activity, reduce radical reaction, while enhancing human body immunity function and raising nucleic acid metabolism level, thus help improving the flight against senium of human body ability, be the approach of a feasible anti-ageing diseases prevention.The special-effect of this oral liquid is with " flavonoid " extensively and efficiently anti-peroxidation, anticancer, antibiosis, antiinflammatory and antiallergic action, strengthens and adjusts immunologic function, improves the nucleic acid metabolism level, promotes that tissue regeneration be basic.

Pheretima claims Lumbricus etc. again, goes the dry body of internal organs and silt to be used as medicine with it, and property is salty-cold, the tool heat clearing away, calm the frightened, relieving asthma, collateral dredging, the effect of invigorating blood circulation, pharmacological action widely [1 ￣ 3] is arranged.We find clinically that earthworm decoction can reduce and ooze out, and shorten inflammatory phase, the healing of accelerated in wounds, and its good antiinflammatory ability is relevant with the state of activation of macrophage.Macrophage is a kind of multi-functional cell, has the chemotactic bactericidal action.

Radix Glycyrrhizae is the dry root and the rhizome of glycyrrhizic legume (Glycyrrhiza uralensis Fisch), Glycyrrhiza glabra L. (G.glabra L.) or Glycyrrhiza inflata Bat. (G.inflata).Its main effective ingredient is triterpene and flavone compound.About showing licoflavone, Radix Glycyrrhizae total flavones and monomeric pharmacological research latest developments thereof have high clinical value to tumor, gastric ulcer and hepatic injury, pathogenic microorganism and to the pharmacological action of aspects such as enzyme.

Radix Scutellariae is a conventional Chinese medicine, and clearing away heat-fire, detoxifcation, hemostasis, effect such as antiabortive are arranged, and its effective ingredient is a flavonoid.The flavones ingredient existing 10 that extracts and identify structure at present from Radix Scutellariae is several, and what wherein content was higher is baicalin and aglycon baicalin thereof, wogonoside and aglycon wogonin thereof.Confirmed on the pharmacology that baicalin has effects such as antibacterial, heat clearing away, blood pressure lowering, calmness, diuresis, function of gallbladder promoting, antiinflammatory antiallergic action, detoxifcation; That Radix Scutellariae have is antibacterial, diuresis, antiinflammatory antiallergic action, function of gallbladder promoting isoreactivity; That wogonin has is antibacterial, diuresis, spasmolysis.Baicalin still is a specificity 12-lipoxygenase inhibitor, and Radix Scutellariae is as Chinese medicine, and its physiologically active is many-sided.Domestic at cellular level also seldom to the physiologically active of the active part flavone of Radix Scutellariae and Its Mechanisms.Except that utilizing its effects for removing toxic heat effect antibiosis anti-inflammatory drug, its blood vessel dilating, anti-oxidation function have great potentiality on as the treating cardiovascular disease medicine; Its antivirus action also is wide in the prospect aspect treatment viral relevant disease such as hepatitis, the acquired immune deficiency syndrome (AIDS).

Propolis is Apis is gathered next viscous secretion resin and the excretory multiple secretions of self mandibular gland of part Cera Flava, pollen and Apis etc. from brood cell, bark or the stem and leaf wound of plant a solid, shaped mixture.Studies have shown that both at home and abroad propolis contains 30 Cera Flavas, 55 Colophonium classes, face cream class, 10 essence oils and 5 pollen, also contain 38 kinds of Flavonoid substances isoreactivity materials.Various bacteria, fungus are had antibacterial action, some virus and protozoacide growth are also had inhibition or killing action, can increase immunity of organisms simultaneously, promote tissue regeneration.Big quantity research has proved that propolis has very high anti-microbial effect.Propolis can suppress the pathogenic effects of 21 kinds of antibacterials (comprising MRSA), 19 kinds of funguses (comprising thrush, tinea and tinea pedis) and 3 kinds of viruses at least.Propolis is anticancer; the anti-peroxidation agent; aspects such as skin and tissue injury's rehabilitation have demonstrated excellent curative; because flavone with high concentration; removing human body endoperoxides free radical, the protection vitamin C is exempted from aspects such as oxidized destruction, enhancing and adjusting human immunologic function and raising nucleic acid metabolism level and is had high medical value.Propolis is used for bronchitis clinically; Influenza and herpes; Tinea and cutaneous fungal infection; Various odontopathy illness; Skin ulcer, burn and pus are swollen; Ear's inflammation; Gastric ulcer, gastritis and colitis; The treatment of multiple illness such as coxitis and colpitic and scapulohumeral periarthritis.

Herba Epimedii is the dry aerial parts of XIAONIE section herbaceos perennial Herba Epimedii.Contain Flavonoid substances such as icariine and noricariin.And contain volatile oil, sterin, alkaloid etc.Have the effect of androgen sample, can promote gonepoiesis.Blood pressure lowering is arranged and increase coronary flow and raising hypoxia-bearing capability, expansion peripheral blood vessel, increase the acra blood flow, microcirculation improvement, and expansion of cerebral vascular, have body's immunity and to promote and dual regulation cerebral blood flow increasing amount, blood fat reducing and blood sugar lowering.Certain antitussive arranged, eliminate the phlegm, relieving asthma, antibacterial, antiinflammatory and obvious sedation.

Fructus Hippophae (Hippophae rhamnoides L.SP.PL) is a defoliation bush plant, belongs to Elaeangnaceae.There is distribution in Yunnan Province of China, Sichuan, deserted mountain, ground such as weak.China is the medical record of Fructus Hippophae country the earliest in the world, is the common drug that Ancient Times in China Tibetan medicine, Mongolian medicine are used for curing the disease.1977, China's Ministry of Public Health was formally listed Fructus Hippophae in " Chinese pharmacopoeia, and be published as the medicine-food two-purpose kind in succession first.A series of research tentative confirmations such as modern nutrition analysis, chemical analysis analysis, pharmacodynamics and toxicology Fructus Hippophae be rich in multivitamin and various trace elements, several amino acids and other multiple biological activity.

Sea buckthorn fruit is sour-sweet, contains rich in protein, in its 20 contained several amino acids, comprises 8 kinds of essential amino acids.Ascorbic content is 3 times of A.chinensis Planch., 20 times of Fructus Crataegi, 100 times of Fructus Mali pumilae.The content of yarn E of supporting one's family is 30 times of Oleum Glycines.The content of vitamin B1 is 2 times of Fructus Fragariae Ananssae; Other also contain vitamin B2, Citrin, folic acid, leaf amide and trace element and unsaturated fatty acid.5-hydroxy tryptamine in the Fructus Hippophae has the notable antitumor activity effect.Unsaturated fatty acid high this 80% (wherein linolenic acid reaches 30%) in the Fructrs Hippophae seed oil.Fructus Hippophae contains 12 kinds of trace element that human body is necessary, and wherein calcium, ferrum, zinc, potassium, selenic content are higher.

Fructus Hippophae has nourishing the liver and kidney, and spleen reinforcing is good for the stomach, the effect of warming the meridian and activating blood circulation.Be rich in the bioactive ingredients of antiinflammatory granulation promoting, radioprotective, antitumor, defying age, arteriosclerosis, anti-multiple senile disease, confirmed by domestic and international expert.To cardiovascular and cerebrovascular disease, cerebral arteriosclerosis, chronic gastritis, gastric ulcer, chronic hepatitis, liver cirrhosis, all have its significant curative effect and aspects such as damage that radiotherapy, chemotherapy are caused and beauty treatment that special role is arranged, be particularly suitable for the old man, women, child are edible.The title that " long-lived precious fruit " arranged.

This oral liquid adopts above-mentioned raw materials, screen its proportioning through comparative study repeatedly, the extraction means, experiment at last shows that they are at defying age, suppress oxidase such as MAO, anti-peroxidation, anticancer, resisting pathogenic microbes, antiinflammatory and antiallergic action strengthen and the adjustment immunologic function, aspect face effects such as raising nucleic acid metabolism level all surpass or reach the effect of former single raw material, in prevention and treatment old common disease hypertension and cardiovascular disease, diabetes, bronchitis, pneumonia, gastric ulcer and hepatic injury, disease such as tumor and multiple cause pathogeny imcrobe infection aspect has high integrated application and is worth.And the primary raw material of this oral liquid mostly is the medicine-food two-purpose kind greatly, thereby has more higher safe and practical value.Therefore say that the significance of this oral liquid is to have proposed a kind of safe and reliable anti-ageing disease-prevention health product new way.Fully absorbing, using on existing various defying age theories and the empirical basis, solving the biochemical synthetic drug in present antisenescence health product market brings potential danger to people a difficult problem.Simultaneously also will improve focusing on of senior health and fitness, life lengthening prevents and cures diseases up.

Embodiment one: immunization of this oral liquid and promoting healing Mechanism Study.Method: give this oral liquid of ip in mice variable concentrations, measure the activation rate of peritoneal macrophage; This oral liquid extracting solution with variable concentrations is cultivated macrophage, measures the quantity of its ability of engulfing dimethyl diaminophenazine chloride and Fc receptor activation.The result: this oral liquid can improve the macrophage activation rate significantly, cell surface Fc function of receptors strengthens, improves the ability that it engulfs dimethyl diaminophenazine chloride.Conclusion: this oral liquid can strengthen the immunocompetence of macrophage significantly, may be to shortening the inflammation cycle, promoting that wound healing plays an important role.

52 of 1 laboratory animals, reagent, instrument Kunming mouse, body weight (22.5 ± 2.5) g, Mus 6 week～8 weeks of age all are ♂.This oral liquid: raw material: get 10 kilograms of (1) Radix Scutellariaes; (2) Pheretima (Lumbricus) is 10 kilograms; (3) Herba Epimedii is 10 kilograms; (4) Radix Glycyrrhizae is 10 kilograms; (5) Fructus Hippophae is 10 kilograms; (6) propolis liquid is 30 kilograms; (7) ethanol is 60 liters; (8) sodium benzoate 100-300g; (9) distilled water adds to 100 liters; Method is: get earlier (1)-after (5) number raw material is cleaned and pulverized, with water retting 30 minutes, adding 5 times of water gagings earlier, decocted 1 hour, totally 2 times, collecting decoction, cross 100 mesh sieves, filtrate has descended concentrating under reduced pressure to become 30 liters in 70 degree, puts cold, under agitation, slowly add ethanol, make the alcohol amount of containing reach 60% (volume fraction), leave standstill cold preservation 48 hours, and filtered, reclaim ethanol, be concentrated into 1: 1, medicinal liquid cold preservation 24 hours is filtered, filtrate under agitation adds strong aqua ammonia and adjusts pH value to 7.0～8.0, and cold preservation 24 hours is filtered, the deammoniation of filtrate heated and stirred, medicinal liquid remain on 100～102 the degree, 2～2.5 hours, to the medicinal liquid pH value be 5.8, cold preservation is spent the night, and filters standby.Get above-mentioned medicinal liquid, adjust pH to 5～7, add 30 liters of sodium benzoate 100 grams and propolis liquid with the 100g/L sodium carbonate liquor, stir, filter, measure content by the G3 sintered glass funnel, add water to 100L again, stir evenly fill, plug plug, roll easy-open lid, sterilization, through quality examination, labeling promptly.Corynebacter Parvum (excellent vaccine detested in abbreviation, the Shanghai institute of Biological Products); Sheep red blood cell (SRBC) (self-control); The anti-sheep red blood cell antibody of rabbit (Sigma).

511 type enzyme micro-plate readers (Shanghai the 3rd analytical tool factory).

2 experimental techniques

2.1 macrophage activation rate

24 of mices are divided into 4 groups at random, this oral liquid 2ml of difference ip variable concentrations, duplicate injection next day, the next day adopt the eye socket depletion method to put to death mice, cut the abdominal cavity open, do the peritoneal fluid sheet, every mice is printed 3 of left, center, right, naturally dry, treat cell fixation after, be Wright-Giemsa dyed blended [4], 100 macrophages of each sample view, counting activating cell and activating cell quantity are not done mathematical statistics, with matched group (is 7.4 phosphate buffers with the 0.02molL-1 pH value) comparison.

The grade scale I type of cell activation degree: the diameter of cell has pseudopodium more than 10 μ m, the granule of engulfing is arranged in the endochylema or phagocytic vacuole is arranged, and this class cell is a utmost point activating cell.The II type: 1. the diameter of cell is more than 10 μ m.2. pseudopodium is arranged.3. the granule of engulfing is arranged in the endochylema or phagocytic vacuole is arranged.All possess last two persons for activating cell.The III type: cell is little, does not have pseudopodium, does not contain and engulfs granule or phagocytic vacuole is arranged, and this class cell is activating cell not.100 macrophages of each sample statistics, it is thin by the third to tell I type, II type, III type, calculates the ratio of I type, II type cell sum and III type cell, does statistical procedures.

2.2 macrophage is to the phagocytosis of dimethyl diaminophenazine chloride

2.2.1 10 ♂ Kunming mouses are selected in the preparation of Turnover of Mouse Peritoneal Macrophages [5] for use, are dipped in volume fraction after disconnected neck marrow is put to death and are in 75% the alcoholic solution to sterilize.Cut off the mouse web portion outer skin,, with syringe peritoneal fluid is extracted out after gently rubbing little shuttle abdominal part to the about 8ml of ip sterilization Hanks liquid, place sterilized test tube, centrifugal, counting, make cell suspension (2 * 106 cells-1) with RPMI 1640 liquid that contain the 100mlL-1 calf serum, this cell suspension is added in 96 culture plates every hole 0.1ml, 37 ℃, 5% carbon dioxide is cultivated 2h, the supernatant that inclines, washed cell is to remove non-adherent cell.

2.2.2 the mensuration of macrophage phagocytic dimethyl diaminophenazine chloride [6] is got cell culture in 96 culture plates, every hole 0.1ml contains 2 * 105 macrophages, through adherent remove non-adherent cell after, add RPMI 1640 culture medium that contain this oral liquid of variable concentrations, 100mlL-1 calf serum, 37 ℃, 5% carbonoxide cultivation 24h, to the dimethyl diaminophenazine chloride physiological salt liquid 100 μ l of every hole adding 0.1%, continue to cultivate 0.5h then.The supernatant that inclines, with potassium phosphate glucose buffer flushing 3 times, every hole adds cytolysate (volume fraction is that 50% acetic acid and volume fraction are 50% dehydrated alcohol) 200 μ l, makes cytolysis.Survey absorbance (A) value at wavelength 550nm place with microplate reader, medication group and matched group are relatively.

2.3 this oral liquid is to the effect of macrophage Fc receptor

2.3.1 18 of the collection mices of macrophage, 5% oral liquid 2ml of lumbar injection, the next day, inject, and adopts the eye socket depletion method to put to death mice behind the 4d, and ip 2ml RPMI 1640 flushing mouse peritoneals are gently rubbed mouse web portion, extract peritoneal fluid out with syringe.Matched group is injected 2ml respectively and is detested excellent vaccine and 2ml 0.02molL-1 pH7.4 phosphate buffer.

2.3.2 the preparation of monolayer macrophage places mouse peritoneal flushing liquor 0.5ml on the coverslip of cleaning, horizontal is cultivated 2h in 37 ℃ of water-bath incubators, macrophage with adhesive capacity is attached on the slide, uses normal saline flushing, remove non-adherent cell and impurity.

2.3.3 the preparation of 80mlL-1 sheep red blood cell (SRBC) is centrifugal in the aseptic bottle of packing into from the blood sample collection from external jugular vein of healthy sheep under aseptic condition, with normal saline cell dilution is formed.

2.3.4 the EA-garland forms test and gets sheep erythrocyte suspension, adds 1: 600 lgG of equivalent, hatches 30min in 37 ℃, removes free lgG 3 times with the normal saline washing, makes the sheep red blood cell (EA) of antibody sandwich.Be made into 5% EA suspension at last, drop on the Cell sheet glass of monolayer macrophage, every agreement that contracts a film or TV play to an actor or actress 0.2ml leaves standstill 2h in 4 ℃ of refrigerators, wash slide then gently to remove free EA.Through fixing, HE dyeing, the EA-garland.Mirror checks down, in around be stained with garland of macrophage work of 5 or 5 above EA.Count 200 macrophages at random, calculate garland and form percentage rate.

3 experimental results

The quantity of I type, II type cell is obviously more than matched group in the experimental group macrophage, and cell bigger has pseudopodium, and born of the same parents include phagocytic vacuole or engulf granule, illustrate that this oral liquid can promote the macrophage activation effect well.

To detest excellent vaccine group similar, the garland of formation is bigger to the influence of Turnover of Mouse Peritoneal Macrophages Fc receptor for this oral liquid extracting solution group, and the EA on the garland is more, with matched group tangible difference is arranged, the percentage rate that the EA garland forms increases significantly, and garland is big, and excellent bacillus is close with detesting.

This oral liquid can promote the macrophage phagocytic dimethyl diaminophenazine chloride significantly.The A value of this oral liquid group is apparently higher than the blank group, even the effect of stronger short macrophage phagocytic dimethyl diaminophenazine chloride is also arranged at very little concentration 1.0mgml-1.

4 discuss

Macrophage is a kind of main immunocyte, and its tool is engulfed foreign body, killed and wounded the effect of antibacterial, also has chemotaxis simultaneously, secrete the multiple factor, make other immunocyte, as activation such as lymphocyte, granulocyte, trend wound location, healing [7] is accelerated in the fellowship inflammatory reaction.After the macrophage activation, cell volume increases, and the acidic hydrolysis enzymatic activity increases, the digestion power of engulfing of killing and wounding with foreign body to antibacterial increases greatly, and can promote neutrophilic granulocyte etc. to the wound location chemotactic, common killer cell, engulfs foreign body, shorten inflammatory phase, promote healing.We select for use the activation rate of macrophage to estimate the immunization of this oral liquid, and the result shows that this oral liquid has the effect that promotes immunocyte-macrophage activation significantly.The action intensity of medicine is followed successively by 50gL-1＞100gL-1＞200gL-1, wherein, preceding two concentration and matched group have significant difference (50gL-1 P＜0.01,100gL-1 P＜0.05), and concentration is that the 200gL-1 medicinal liquid also has activation (data does not show) to macrophage.Drug level is too high or too low all can not to be promoted only could promote the activation of macrophage a little less than the activation or effect of macrophage when concentration is moderate, and test and point out, this oral liquid has dual regulation to macrophage.

4.2 the Fc receptor is one group of most important surface marker of macrophage, its functional status can change with the immune level of body.As indicator cells, make itself and the anti-sheep red blood cell (SRBC) antibodies that adapts form EA with sheep red blood cell (SRBC).Macrophage can combine with EA by the Fc receptor and form garland.The immune level that quantity that its garland forms and activity change can be reacted body to a certain extent.Macrophage Fc receptor binding antibodies forms antigen antibody complex, borrows the Fab section of antibody to combine with antibacterial again, around macrophage, makes antibacterial-macrophage-antibody " bridging ".Working in coordination with down of C3b receptor, make macrophage activation and bring into play powerful opsonic action, suppress or killing bacteria.Therefore, the quantity of macrophage Fc receptor and activity change can be used as an index that detects the macrophage vigor.Experiment shows: this oral liquid can promote the activation of macrophage Fc receptor significantly, strengthens the ability that it engulfs dimethyl diaminophenazine chloride.Embodiment two: this oral liquid treatment old people respiratory tract bacterial infection purpose: estimate the clinical efficacy and the safety thereof of this oral liquid sheet treatment respiratory tract infection.Method: adopt single blind contrast method, 104 routine respiratory tract bacterial infection patients are matched and are randomized into test and matched group.Test group gives 20 milliliters of this oral liquids, every day secondary, matched group gives erythromycin ethylsuccinate 0.375g, tid, po.Be 7～10d the course of treatment.The result: the comprehensive clinical efficacy of test group significantly is better than matched group (P＜0.01); The test group cure rate is 71.2%, and effective percentage is 94.2%, all is significantly higher than matched group (P＜0.05); Test group is not seen untoward reaction, and matched group untoward reaction rate is 15.4%.Conclusion: the treatment that this oral liquid is used for respiratory tract bacterial infection is effective, safe.Materials and methods 1 case is selected

Observed object is through acute respiratory bacterial infection patients clinical and that bacteriology checking is made a definite diagnosis.48～70 years old age, male or female.Accepting effective antibacterial drug therapy person will not be selected in.MethodsThe cases enrolled 104 examples.In test group (T) 52 examples, male 26 examples, women 26 examples, year mean age (55.48 ± 14.52); The state of an illness is light, in, severe person is respectively 9,31 and 12 examples, mean body temperature is (37.64 ± 0.69) ℃, average weight (59.60 ± 8.50) kg; The sick kind: upper respiratory tract infection 28 examples, lower respiratory infection 11 examples, bacterial pneumonia 13 examples.Among matched group (C) the 52 routine patients, male 23 examples, women 29 examples, mean age (56.07 ± 14.04) year, the state of an illness is light, in, heavy person is respectively 14,28 and 10 examples, mean body temperature (37.73 ± 0.82) ℃, average weight (57.72 ± 6.59) kg; The sick kind: upper respiratory tract infection 28 examples, lower respiratory infection 29 examples, bacteriology's pneumonia 4 examples.Learn check by statistics, relevant symptom, sign before the patient treatment are as there was no significant difference between the average score value of single indexs such as coughing, cough up phlegm, dyspnea, pulmonary rale, pharyngalgia, antiadoncus, submandibular lymph nodes swell and ache.2 medications

T group patient gives this oral liquid, 20 milliliters, every day secondary, its preparation method is with embodiment one.C group patient give the erythromycin ethylsuccinate that Xi'an pharmaceutical factory produces (the 0.125g/ sheet, trade name: erythromycin ethylsuccinate) 3, tid, po.3 observation items

3.1 all are tried forward and backward blood, the routine urinalysis all done of patient treatment, liver, renal function and chest X-ray lab testing.

3.2 all are tried that patient treatment is forward and backward gathers all that the deep sputum specimen is made antibacterial culturing and kind is identified bacteriology checking.Adopt paper disk method to make the sensitization test of medicines such as this oral liquid, erythromycin, norfloxacin, cefazolin sodium, gentamycin and benzylpencilline.The result presses the NCCLS standard and judges.And this oral liquid, MIC50 and the MIC90 value of erythromycin have been detected to isolating pathogenic bacterium.

3.3 all situations of change of being tried the forward and backward clinical symptoms of patient treatment, sign are observed and write down to clinical indexes day by day.Cough, cough up phlegm, the record of dyspnea and pulmonary rale adopts scoring method: do not have-, slight+, moderate, severe.All untoward reaction that tried to occur among the patient of observed and recorded, comprise its clinical manifestation, degree, generation and persistent period, handle and lapse to, and judge which kind of situation it is listed as with being subjected to the subordinate that concerns between the reagent thing: 1. relevant certainly, 2. may be relevant, 3. irrelevant certainly, 4. may have nothing to do, 5. be difficult to judge.Calculate adverse reaction rate according to the 1. and 2. case load of two kinds of situations.4 curative effect judging standards

" the antibacterials clinical research guideline " that clinical efficacy was issued by Ministry of Public Health in 1993 evaluated by recovery from illness, produce effects, progress, invalid 4 grades.Add up to effectively with recovery from illness and produce effects, calculate effective percentage in view of the above.

Bacteriology's curative effect divides antibacterial to remove, remove, part is removed, replace, infect (treatment is separated to a kind of new pathogenic bacterium when finishing, and the sings and symptoms that infects occurs, need give therapist) 5 grades.5 statistical methods

Adopt χ 2 or accurately probabilistic method check, the sxemiquantitative data adopts rank test, and enumeration data adopts the t check.The comprehensive clinical efficacy of 1 clinical efficacy sees attached list 1 as a result.Through rank test, T group patient's comprehensive clinical efficacy is higher than C group (P＜0.01) significantly.The chi-square criterion result shows that the cure rate (71.2%) of T group also is higher than C group (40.4%), P＜0.01 significantly.

1.2 the curative effect of single index 1. behind the T group patient treatment the average decline branch (2.09) of cough symptom significantly greater than C group (1.60), P＜0.05; 2. the cough disappearance rate (75.0%) of symptom and the disappearance rate (88.0%) of pulmonary rale of T group patient is significantly higher than C group (42.4% and 40.0%) respectively, and the P value is equal＜and 0.01; 3. the T group patient average disappearance natural law of coughing up phlegm be (5.87 ± 2.34) d, significantly is less than C and organizes (8.07 ± 2.75) d, P＜0.05.2 bacteriology's curative effects

104 examples are tried to isolate altogether among the patient 105 strain pathogenic bacterium.These bacterial strains are respectively 98.8%, 90.1%, 84.0%, 85.2%, 72.8% and 49.4% to the responsive rate of this oral liquid, erythromycin, norfloxacin, cefazolin sodium, gentamycin and benzylpencilline.

This oral liquid MIC50 and MIC90 value are respectively 0.25mg/L and 8.00mg/L, and erythromycin is respectively 0.50mg/L and 16.0mg/L to MIC50 and the MIC90 value of these group pathogenic bacterium.The former only is the latter half.

This oral liquid sheet is 94.2% to the clearance rate of 52 routine pathogenic bacterium, and the erythromycin ethylsuccinate sheet is 88.7% to the clearance rate of 53 strain pathogenic bacterium.Do not see the case that flora is replaced and infected among this group patient.Antibacterial is on average removed natural law and is respectively (5.41 ± 2.14) d and (5.39 ± 1.90) d, P＞0.05 after two groups of patient's medications.3 untoward reaction

T organizes and untoward reaction do not occur after 52 routine patients take medicine.After C organized 52 routine patients and takes medicine, 9 example time untoward reaction, adverse reaction rate 17.3% appearred in 8 people.These untoward reaction are all slight, of short duration, need not special handling, and also influence does not continue medication.The unusual of blood, routine urinalysis regulating liver-QI, kidney function test all do not appear after two groups of patients of T, C take medicine.

Erythromycin ethylsuccinate is the ethyl succinate of erythromycin, is not destroyed by gastric acid, and it is its advantage that liver decreases incidence rate low.Therefore, we select the contrast medicine of this medicine as this oral liquid.

This result of the test demonstrates this oral liquid clinical efficacy and is better than erythromycin ethylsuccinate.This is consistent with bacteriology's index testing result.This oral liquid all has definite curative effect to upper and lower respiratory tract infection and pneumonia, and this all has powerful inhibitory action relevant with this oral liquid to the 50s subunits of most of pathogenic bacterium of the acquired respiratory tract infection of community.

This result of the test shows that also this oral liquid has safety preferably.This medicine is to liver, renal function, and blood, routine urianlysis result do not produce harmful effect.Embodiment three: this oral liquid is regulated the clinical research object and method 1 case of old people's blood fat and is selected

In JIUYUE, 1996～1997 year June, check serum total cholesterol (TC) 〉=5.69mol/L and (or) triglyceride (TG) 〉=1.76mmol/L and (or) HDL-C (HDL-C) male≤1.04mmol/L, in the hyperlipidemia patient of women≤1.17mmol/L, through the inquiry medical history, health check-up and relevant check, remove nephropathy, liver, gallbladder, pancreas and thyroid illness, unsteered diabetes, the medicine hyperlipemia, once suffered from myocardial infarction in half a year, cerebrovascular, severe trauma, capital operation and once taking in 8 weeks influences outside the medicine of the blood lipid level person, and totally 114 examples enter this research.Wherein male 69 examples, women 45 examples, 47～73 years old age, average 57 years old.Accompanied with hypertension person 57 examples, coronary disease patient 66 examples, glycosuria patient 4 examples.2 medications

Be divided into 2 groups at random by serum TC and TG level: 57 examples are organized in treatment, obey this oral liquid, and 20 milliliters/time, each 1 time sooner or later.Matched group 57 examples, clothes Cebo-Caps, 1/time, bid.Be for 8 weeks the course of treatment.

Take medicine preceding and take medicine the back 4 and 8 weekends, all inquire symptom, detect body weight, blood pressure, heart rate, the rhythm of the heart, liver palpation of spleen, look into electrocardiogram, glucose in urine, blood and routine urinalysis, platelet count and empty stomach extracting vein blood and survey blood fat, apolipoprotein, lipoprotein (a) (Lp (a)), the red matter of gallbladder, ALT, blood glucose, BUN or creatinine.3 blood fat, apolipoprotein and Lp (a) measure

Get the preceding last a meal alcohol prohibition of blood and avoid high fat diet, the above extracting vein blood of empty stomach 12h, timely separation of serum is got behind the blood in the 6h with speed immunochemistry turbidimetry, finishes apolipoprotein A-1 (ApoA-I) and apolipoprotein B (ApoB) mensuration with Beckman company " ICS " instrument and matched reagent.Residue serum places film seal in vitro, stores in-20 ℃ of refrigerators, and enzyme reagent and method with Shanghai biochemical reagents institute supply in the 72h are carried out TC, TG and HDL-C mensuration.

The CV value of relevant mensuration all＜5%, Lp (a) measures with the double-antibody sandwich elisa method, CV value is 5.2% in batch, the CV value is 9.9% between criticizing.LDL-C computing formula following (unit is mmol/L): LDL-C=TC-HDL-C-TG/2.24 statistical analysis

With t check, chi-square criterion and variance analysis.Blood fat, lipoprotein, apolipoprotein and Lp (a) level see attached list 2 before and after 1 treatment as a result.The numerical value of every index before the treatment does not all have significant difference (P＞0.05) in the table between two groups.Thus, the numerical value between two groups has comparability after the treatment.2 individual efficacy analysis see attached list 3.The prompting of this result of study is discussed, and this oral liquid has good and blood lipid regulation effect reliably, and the pantethin of its regulating lipid and report, etofylline clofibrate and olbetam are suitable.Efficacy analysis after two groups of patient's medications of subordinate list 1T, C

The sick kind	Grouping	(comprehensive clinical efficacy)/(the invalid total of recovery from illness produce effects progress)					(cure rate)/((%))	(effective percentage)/((%))
									Acute pharyngitis	?T	????4	????2	????0	????0	????6	????66.7	?????100.0
	?C	????7	????3	????0	????0	????10	????70.0	?????100.0
									Acute tonsillitis	?T	????9	????3	????0	????0	????12	????75.0	?????100.0
	?C	????7	????3	????1	????0	????11	????63.6	?????90.9
									Acute bronchitis	?T	????9	????0	????0	????1	????10	????90.0	?????90.0
	?C	????4	????3	????0	????0	????7	????57.1	?????100.0
									The chronic bronchitis acute attack	?T	????3	????5	????1	????0	????9	????33.3	?????88.9
	?C	????1	????7	????6	????0	????14	????7.10	?????57.1
									Prop up and expand concurrent infection	?T	????0	????1	????1	????0	????2	????0	?????50
	?C	????0	????3	????3	????0	????6	????0	?????50
									Pneumonia	?T	????12	????1	????0	????0	????13	????92.3	?????100.0
	?C	????2	????2	????0	????0	????4	????50	?????100.0
									Add up to	?T	????37	????12	????2	????1	????52	????71.2	?????94.2
	?C	????21	????21	????10	????0	????52	????40.4	?????80.8

Serum lipids before and after subordinate list 2 treatments, lipoprotein, apolipoprotein and Lp (a) level

Index	Group	The example number	Before the treatment (± S)	Difference (%) before and after the treatment
						4 weeks	8 weeks
				TC(mmol/L)	Treatment			38	??7.00±1.10	?????-0.53(-7.6) b	????-0.89(-12.7) cf
	Contrast	38	??6.88±1.00			?????-0.18(-2.6)	????+0.31(+4.5) a
				LDL-C(mmol/L)	Treatment			38	??4.42±1.16	?????-0.28(-6.3) a	????-0.45(-10.1) a
	Contrast	38	??4.41±1.14			?????-0.19(-4.4)	????-0.07(-1.6)
				HDL-C(mmol/L)	Treatment			40	??0.91±0.14	?????+0.07(+7.5) b	????+0.11(+11.7) b
	Contrast	36	??0.91±0.13			?????-0.02(-2.4)	????+0.01(+0.7)
				TC/HDL-C	Treatment			57	??6.30±1.45	?????-0.53(-8.3) be	????-1.00(-15.9) cf
	Contrast	57	??6.26±1.57			?????+0.49(+7.8) a	????0.71(+11.3) b
				TG(mmol/L)	Treatment			50	??8.19±3.05	?????-1.43(-17.4) ε	????-2.83(-34.7) cf
	Contrast	50	??7.44±3.28			?????+0.68(+9.2)	????+2.43(+32.7) c
				ApoA-I(mg/dl)	Treatment			57	??103.9±15.2		????+4.5(+4.3)
	Contrast	57	??102.3±16.6				????+0.4(+0.4)
				ApoB(mg/dl)	Treatment			57	??123.3±25.0		????-8.1(-6.6) a
	Contrast	57	??129.8±28.8				????-8.1(-6.2) b
				ApoA-I	Treatment			57	??0.87±0.18		????+0.12(+13.3) c
ApoB	Treatment	57	??0.82±0.21				????+0.05(+6.1) a
				Lp(a)(mg/dl)	Treatment			33	??220.0±160.9		????+9.6(+4.4)
	Contrast	34	??241.7±182.3				????-8.6(-3.6)

With control before than a: P＜0.05; B: P＜0.01; C: P＜0.001.Compare between group: d: P＜0.05; E: P＜0.01; F: P＜0.001.+ show rising ,-decline shown.The individuality of subordinate list 3 blood lipid regulation is imitated and is analyzed

Index	Take medicine (week)	Group	Sum	Example number (%)					P
										Produce effects	The middle effect	Effectively	Invalid	Effectively total
								??(≥15％)							??(10％～＜15％)	???(5％～＜10％)	??(＜5％)	??(≥5％)
	??4	Treatment	38						??8(21.1)	??4(10.5)	???7(18.4)	??19(50.0)	??19(50.0)	NS
				?TC	??4	Contrast	38	??4(10.5)							??4(10.5)	???6(15.8)	??24(63.2)	??14(36.8)
	??8	Treatment	38						??12(31.6)	??5(13.2)	???7(18.4)	??14(36.8)	??24(63.2)	＜0.001
					??8	Contrast	38								??2(5.3)	???6(15.8)	??30(78.9)	??8(21.1)
									??(≥30％)	??(20％～＜30％)	???(10％～＜20％)	??(＜10％)	??(≥10％)
					??4	Treatment	50	??17(34.0)							??6(12.0)	???7(14.0)	??20(40.0)	??30(60.0)	＜0.01
?TG	??4	Contrast	50						??5(10.0)	??3(6.0)	???9(18.0)	??33(66.0)	??17(34.0)
					??8	Treatment	50	??24(48.0)							??10(20.0)	???8(16.0)	??8(16.0)	??42(84.0)	＜0.001
	??8	Contrast	50							??2(4.0)	???2(4.0)	??46(92.0)	??4(8.0)
								??(≥0.26) a							??(0.18～＜0.26) a	???(0.10～＜0.18) a	??(＜0.10) a	??(≥0.10) a
	??4	Treatment	40						??3(7.5)	??5(12.5)	???6(15.0)	??26(55.0)	??14(35.0)	＜0.05
				?HDL-C	??4	Contrast	36	??2(5.6)							??2(5.6)	???4(11.1)	??28(77.7)	??8(22.3)
	??8	Treatment	40						??7(17.5)	??6(15.0)	???5(12.5)	??22(55.0)	??18(45.0)	＜0.05
					??8	Contrast	36								??2(5.6)	???6(16.7)	??28(77.7)	??8(22.3)

A: unit: mmol/L, () bracket inner digital is the evaluation criterion of therapeutical effect.The NS not statistically significant.

Claims (2)

1, use health care oral liquid a kind of old age, it is characterized in that it is the externally-applied ointment of being made by the following weight proportion raw material:

(1) Radix Scutellariae is 10～20 kilograms

(2) Pheretima (Lumbricus) is 10～20 kilograms

(3) Herba Epimedii is 10～20 kilograms

(4) Radix Glycyrrhizae is 10～20 kilograms

(5) Fructus Hippophae is 10～20 kilograms

(6) propolis liquid is 30～60 kilograms

(7) ethanol is 60～120 liters;

(8) sodium benzoate 100-300g;

(9) distilled water adds to 100～200 liters;

2, preparation method according to claim 1 is characterized in that: get earlier (1)-after (5) number raw material is cleaned and pulverized, earlier with water retting 30 minutes, add 5 times of water gagings, decocting totally 2 times 1 hour, collecting decoction is crossed 100 mesh sieves, and filtrate has descended concentrating under reduced pressure to become 30 liters in 70 degree, put coldly, under agitation, slowly add ethanol, make the alcohol amount of containing reach 60% (volume fraction), leave standstill cold preservation 48 hours, and filtered, reclaim ethanol, be concentrated into 1: 1, medicinal liquid cold preservation 24 hours is filtered, and filtrate under agitation adds strong aqua ammonia and adjusts pH value to 7.0～8.0, cold preservation 24 hours, filter, the deammoniation of filtrate heated and stirred, medicinal liquid remains on 100～102 degree, 2～2.5 hours, to the medicinal liquid pH value be 5.8, cold preservation is spent the night, and filters standby.Get above-mentioned medicinal liquid, adjust pH to 5～7, add 30 liters of sodium benzoate 100 grams and propolis liquid with the 100g/L sodium carbonate liquor, stir, filter, measure content by the G3 sintered glass funnel, add water to 100L again, stir evenly fill, plug plug, roll easy-open lid, sterilization, through quality examination, labeling promptly.