CN1240296C - Method for producing diary riched in conjugation linoleic acid - Google Patents
Method for producing diary riched in conjugation linoleic acid Download PDFInfo
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- CN1240296C CN1240296C CN 02144916 CN02144916A CN1240296C CN 1240296 C CN1240296 C CN 1240296C CN 02144916 CN02144916 CN 02144916 CN 02144916 A CN02144916 A CN 02144916A CN 1240296 C CN1240296 C CN 1240296C
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Abstract
The present invention relates to a method for producing a milk product rich in conjugation linoleic acid. Milk produced by the method is rich in conjugation linoleic acid. The method has the following technical scheme: a. peptone, beef extract, yeast extractive, diammonium citrate, sodium acetate, glucose, tween 80, seven-hydrate containing magnesium sulfate and four-hydrate containing manganese sulfate are mixed with each other, and calcium carbonate is then added; distilled water is added until one liter of mixture is obtained, and the PH value is adjusted to 6.2 to 6.4 so that a culture medium (1) is obtained; b. after fresh milk is sterilized and disinfected, sterilized oxygen ferric protoporphyrin, milk powder, glucose and buffer solution of potassium phosphate are added, and a culture medium (2) is obtained through the processes; and c. a melting fermentation agent is inoculated into the culture medium (1) so as to be cultivated into an inoculum, the inoculum is inoculated into the culture medium (2), and melon seed oil is added before cultivation ends. Consequently, the milk product rich in conjugation linoleic acid is obtained.
Description
Technical field: the present invention relates to the method that the CLA dairy produce is rich in a kind of production.
Background technology: contain in the sour milk that existing manufacturing technique is produced at present along 9, the concentration of anti-11-CLA only is 0-2 milligram/gram fat, can not reach the needed amount of its physiological action of performance.
Summary of the invention: the technical problem to be solved in the present invention provides a kind of method of producing sour milk product, and the milk of being produced by this method is rich in CLA.Technical scheme: a, add calcium carbonate, adding distil water to a liter after peptone 8-12 gram, beef extract 8-12 gram, yeast extract 3-6 gram, dibasic ammonium citrate 1-3 gram, sodium acetate, glucose, Tween 80, the magnesium sulfate that contains seven water, the manganese sulfate that contains four water mixed, the accent pH value is 6.2-6.4, sterilized 15 minutes, and obtained culture medium (1); B, with behind the fresh milk sterilization, the oxygen protoferriheme 1-5 mcg/ml that adds sterilization, milk powder, glucose and buffer solution of potassium phosphate, wherein milk powder account for the culture medium weight ratio be 0.5-3%, glucose account for the culture medium weight ratio be 0.05-0.2%, buffer solution of potassium phosphate 50-100 mM/liter, obtain culture medium (2) through aforementioned process; C, the leavening that melts is inoculated in the culture medium (1) that obtains previously, cultivate 8-12 hour as inoculum in the 35-38 degree, inoculum is inoculated in the aforesaid culture medium (2), inoculum concentration is 7-10%, cultivated 10-12 hour in the 35-38 degree, before cultivate finishing 30-60 minute, adding 0.2-0.5 grams per liter Semen Benincasae oil promptly obtained being rich in the CLA dairy produce.Beneficial effect: contain in the sour milk that production technology of the present invention is simple, with short production cycle, this explained hereafter goes out along 9, anti-11-cla concn is more than 10 milligrams/gram fat, and the composition of the isomer of CLA is similar to natural dairy produce, wherein contain along 9, anti-11-CLA accounts for the 85-90% of total amount, and is nontoxic through the product safety of this technology gained, is of value to healthy, do not influence the nutritional labeling of sour milk, do not influence the sensory trait of sour milk.
The specific embodiment: add calcium carbonate 3 grams, adding distil water to a liter after peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, dibasic ammonium citrate 2 grams, sodium acetate 5 grams, glucose 21 grams, 1 milliliter of Tween 80, magnesium sulfate (MgSO47H2O) 0.58 gram that contains seven water, manganese sulfate (MnSO4 4H2O) 0.25 gram that contains four water mixed, the accent pH value is 6.2-6.4,121 degree sterilizations 15 minutes obtain culture medium (1); Fresh milk is spent the pasteurizations sterilization after 30 seconds in 72, the oxygen protoferriheme 1-5 mcg/ml that adds sterilization, milk powder, glucose and buffer solution of potassium phosphate, wherein milk powder accounts for that the culture medium weight ratio is 1%, glucose accounts for that the culture medium weight ratio is 0.1%, buffer solution of potassium phosphate 100 mMs/liter, oxygen protoferriheme solution concentration is 2%, with the preparation of 0.1%KOH solution, obtain culture medium (2) through aforementioned process; Leavening is stored in bacterium-80 degree with the culture medium (1) that contains 10% glycerine, the leavening that melts is inoculated in the culture medium (1) that obtains previously, said leavening is lactobacillus acidophilus (Lactobacillus acidophilus) or lactobacillus plantarum (Lactobacillus plantarum) or lactobacillus bulgaricus (Lactobacillus bulgaricus sp), cultivate 10 hours as inoculum in the 35-38 degree, inoculum is inoculated in the aforesaid culture medium (2), inoculum concentration is 8%, cultivated 11 hours in the 35-38 degree, cultivating preceding 50 minutes of end, add 0.4 grams per liter Semen Benincasae oil, promptly obtain being rich in the CLA dairy produce.
Claims (2)
1, the method for CLA dairy produce is rich in a kind of production, it is characterized in that: a, add calcium carbonate 3 grams, adding distil water to a liter after peptone 8-12 gram, beef extract 8-12 gram, yeast extract 3-6 gram, dibasic ammonium citrate 1-3 gram, sodium acetate 5 grams, glucose 21 grams, 1 milliliter of Tween 80, magnesium sulfate 0.58 gram that contains seven water, manganese sulfate 0.25 gram that contains four water mixed, the accent pH value is 6.2-6.4, sterilized 15 minutes, and obtained culture medium (1); B, with behind the fresh milk sterilization, the oxygen protoferriheme 1-5 mcg/ml that adds sterilization, milk powder, glucose, buffer solution of potassium phosphate, wherein milk powder account for the culture medium weight ratio be 0.5-3%, glucose account for the culture medium weight ratio be 0.05-0.2%, buffer solution of potassium phosphate 50-110 mM/liter, obtain culture medium (2) through aforementioned process; C, the leavening that melts is inoculated in the culture medium (1) that obtains previously, cultivate 8-12 hour as inoculum in the 35-38 degree, inoculum is inoculated in the aforesaid culture medium (2), inoculum concentration is 7-10%, cultivated 10-12 hour in the 35-38 degree, before cultivate finishing 30-60 minute, adding 0.2-0.5 grams per liter Semen Benincasae oil promptly obtained being rich in the CLA dairy produce.
2, the method for CLA dairy produce is rich in production according to claim 1, it is characterized in that: said leavening is lactobacillus acidophilus or lactobacillus plantarum or lactobacillus bulgaricus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02144916 CN1240296C (en) | 2002-09-03 | 2002-12-12 | Method for producing diary riched in conjugation linoleic acid |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02132878 | 2002-09-03 | ||
| CN02132878.1 | 2002-09-03 | ||
| CN 02144916 CN1240296C (en) | 2002-09-03 | 2002-12-12 | Method for producing diary riched in conjugation linoleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1480052A CN1480052A (en) | 2004-03-10 |
| CN1240296C true CN1240296C (en) | 2006-02-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02144916 Expired - Fee Related CN1240296C (en) | 2002-09-03 | 2002-12-12 | Method for producing diary riched in conjugation linoleic acid |
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| CN (1) | CN1240296C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100527966C (en) * | 2006-09-07 | 2009-08-19 | 广州风行牛奶有限公司 | Method for adding polyunsaturated fatty acid in animal milk |
| CN101849933A (en) * | 2010-04-15 | 2010-10-06 | 广东海洋大学 | Conjugated linoleic acid ferrite preparation and preparation method thereof |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100448356C (en) * | 2006-07-05 | 2009-01-07 | 内蒙古蒙牛乳业（集团）股份有限公司 | A cow milk containing conjugated linoleic acid |
| CN101057611B (en) * | 2007-05-25 | 2014-11-05 | 中国农业科学院农产品加工研究所 | Cheese and its preparing process |
| CN101755911B (en) * | 2009-12-23 | 2014-04-23 | 大连紫宁科技发展有限公司 | Production of yogurt rich in conjugated linoleic acid by using immobilization technology and method thereof |
| CN106753752A (en) * | 2016-12-13 | 2017-05-31 | 青岛嘉瑞生物技术有限公司 | A kind of health-care salicorne oil beneficial to hypotensive |
| CN106753751A (en) * | 2016-12-13 | 2017-05-31 | 青岛嘉瑞生物技术有限公司 | A kind of glasswort nutrition oil for alleviating fatty liver |
-
2002
- 2002-12-12 CN CN 02144916 patent/CN1240296C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100527966C (en) * | 2006-09-07 | 2009-08-19 | 广州风行牛奶有限公司 | Method for adding polyunsaturated fatty acid in animal milk |
| CN101849933A (en) * | 2010-04-15 | 2010-10-06 | 广东海洋大学 | Conjugated linoleic acid ferrite preparation and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1480052A (en) | 2004-03-10 |
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