CN103444784B - A kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention - Google Patents
A kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention Download PDFInfo
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Abstract
The invention provides a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention, the concrete steps of its preparation method: the Lactobacterium acidophilum in freezing pipe, bacillus aceticus, Rhodopseudomonas palustris, yeast saccharomyces cerevisiae are activated respectively in inclined-plane, purifying obtains each slant strains afterwards, for subsequent use; Each slant strains is utilized to prepare corresponding original bacteria liquid; Each original bacteria liquid is inoculated in sterilized fermention medium I by the bacterium amount ratio of 1:1:1:1 and cultivates, obtain the first order seed of compound micro-ecological preparation; By first order seed by 5% inoculum size be inoculated in sterilized fermention medium II and cultivate to obtain the secondary seed of compound micro-ecological preparation; Finally by secondary seed by 5% inoculum size be seeded to the finished product that sterilized fermention medium III cultivates to obtain compound micro-ecological preparation.Utilize compound micro-ecological preparation of the present invention can play at pig breeding farm the effect intercepting pathogen transmission, thus improve pig-breeding resistance against diseases.
Description
[technical field]
The present invention relates to a kind of compound micro-ecological preparation cultivating field, particularly relate to a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention.
[background technology]
Along with centralization day by day, the large-scale development of pig-breeding, the requirement that the epidemic prevention and control of live pig controls is improved day by day.At present, the epidemic prevention and control on pig farm mainly still uses the traditional means such as vaccine and microbiotic, and long-term antibiotic usage also brings obvious drawback, not only aquaculture cost improve, and bring live pig drug resistance problems and because of pork antibiotic remains bring to health of people threaten problem.Therefore, the live pig epidemic prevention technology studying antibiotic-free type becomes one of topic of current cultivation industry hottest point.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention, utilizes it can play at pig breeding farm the effect intercepting germ and pass, thus improves the resistance against diseases of pig-breeding.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention, and the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Lactobacterium acidophilum in freezing pipe, bacillus aceticus, Rhodopseudomonas palustris, yeast saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain Lactobacterium acidophilum slant strains, bacillus aceticus slant strains, Rhodopseudomonas palustris slant strains and yeast saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: Lactobacterium acidophilum slant strains be inoculated in sterilized liquid nutrient medium I, be placed on 37 DEG C at Anaerobic culturel 1-2 days obtain Lactobacterium acidophilum original bacteria liquid; Bacillus aceticus slant strains be inoculated in sterilized liquid nutrient medium II, then at 30 DEG C, aerobic cultivation obtains bacillus aceticus original bacteria liquid in 1-2 days; Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium III, then within illumination cultivation 5-7 days, obtains Rhodopseudomonas palustris original bacteria liquid in 28 DEG C-30 DEG C anaerobism tengsten lamps; Yeast saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium IV, at 28 DEG C-30 DEG C, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: Lactobacterium acidophilum original bacteria liquid, bacillus aceticus original bacteria liquid, Rhodopseudomonas palustris original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermention medium I by the bacterium amount ratio of 1:1:1:1 and cultivate, particularly: first inoculate bacillus aceticus original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days at 30 DEG C, inoculate Lactobacterium acidophilum original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, at 30 DEG C, Anaerobic culturel 3 days, obtains the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum size be inoculated in sterilized fermention medium II, and at 30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum size be seeded in sterilized fermention medium III, and at 30 DEG C first aerobic 3-5 days Anaerobic culturel 3-5 days again, obtain the finished product of compound micro-ecological preparation;
Wherein, the component of described liquid nutrient medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, 0.2 gram, magnesium sulfate, manganous sulfate 0.05 gram, 20 grams, calcium carbonate, 15 grams, agar, water 1000ml, pH6.8; The component of described liquid nutrient medium II: yeast powder 10 grams, glucose 10, dipotassium hydrogen phosphate 0.5 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH5.5; The component of described liquid nutrient medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH 7.0-7.2; The component of described liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, pH nature; The component of described fermention medium I: tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, excess water; The component of described fermention medium II: tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water; The component of described fermention medium III: tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the concrete operations that in described step (1), Lactobacterium acidophilum activates in inclined-plane are: be inoculated on sterilized slant medium I with transfering loop by Lactobacterium acidophilum, afterwards Anaerobic culturel 1-2 days at 37 DEG C; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, 0.2 gram, magnesium sulfate, manganous sulfate 0.05 gram, 20 grams, calcium carbonate, 15 grams, agar, water 1000ml, pH6.8.
Further, the concrete operations that in described step (1), bacillus aceticus activates in inclined-plane are: prepare slant medium II, and sterilizing 20min at slant medium II is placed in 121 DEG C, then be cooled to less than 60 DEG C and add dehydrated alcohol, and every 1000ml slant medium II need add 100ml dehydrated alcohol, with transfering loop, bacillus aceticus is inoculated on slant medium II afterwards, then aerobic cultivation 1-2 days at 30 DEG C; The component of described slant medium II: yeast powder 10 grams, glucose 10, dipotassium hydrogen phosphate 0.5 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH5.5.
Further, the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: be inoculated on sterilized slant medium III with transfering loop by Rhodopseudomonas palustris, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 15min; And the component of this slant medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH 7.0-7.2.
Further, the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: be inoculated on sterilized slant medium IV with transfering loop by yeast saccharomyces cerevisiae, aerobic cultivation 3 days at 28-30 DEG C afterwards; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, pH nature.
The beneficial effect of a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention of the present invention is: adopt compound micro-ecological preparation of the present invention can form a microenvironment based on Benign microbes at plant's periphery, obstruct pathogen transmission can be played, thus improve the effect of live pig resistance against diseases, also have easy to use, act on obvious feature; And this compound micro-ecological preparation also has the advantage that technique is comparatively simple, cost is low, thus compound micro-ecological preparation of the present invention can be applied comparatively widely.
[embodiment]
A kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention of the present invention, the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Lactobacterium acidophilum in freezing pipe, bacillus aceticus, Rhodopseudomonas palustris, yeast saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain Lactobacterium acidophilum slant strains, bacillus aceticus slant strains, Rhodopseudomonas palustris slant strains and yeast saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: Lactobacterium acidophilum slant strains be inoculated in sterilized liquid nutrient medium I, be placed on 37 DEG C at Anaerobic culturel 1-2 days obtain Lactobacterium acidophilum original bacteria liquid; Bacillus aceticus slant strains be inoculated in sterilized liquid nutrient medium II, then at 30 DEG C, aerobic cultivation obtains bacillus aceticus original bacteria liquid in 1-2 days; Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium III, then within illumination cultivation 5-7 days, obtains Rhodopseudomonas palustris original bacteria liquid in 28 DEG C-30 DEG C anaerobism tengsten lamps; Yeast saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium IV, at 28 DEG C-30 DEG C, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: Lactobacterium acidophilum original bacteria liquid, bacillus aceticus original bacteria liquid, Rhodopseudomonas palustris original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermention medium I by the bacterium amount ratio of 1:1:1:1 and cultivate, particularly: first inoculate bacillus aceticus original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days at 30 DEG C, inoculate Lactobacterium acidophilum original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, at 30 DEG C, Anaerobic culturel 3 days, obtains the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum size be inoculated in sterilized fermention medium II, and at 30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum size be seeded in sterilized fermention medium III, and at 30 DEG C first aerobic 3-5 days Anaerobic culturel 3-5 days again, obtain the finished product of compound micro-ecological preparation.
Wherein: the concrete operations that in step (1), Lactobacterium acidophilum activates in inclined-plane are: be inoculated on sterilized slant medium I with transfering loop by Lactobacterium acidophilum, afterwards Anaerobic culturel 1-2 days at 37 DEG C; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, 0.2 gram, magnesium sulfate, manganous sulfate 0.05,20 grams, calcium carbonate, 15 grams, agar, water 1000ml, pH6.8.The concrete operations that in step (1), bacillus aceticus activates in inclined-plane are: prepare slant medium II, and sterilizing 20min at slant medium II is placed in 121 DEG C, then be cooled to less than 60 DEG C and add dehydrated alcohol, and every 1000ml slant medium II need add 100ml dehydrated alcohol, with transfering loop, bacillus aceticus is inoculated on slant medium II afterwards, then aerobic cultivation 1-2 days at 30 DEG C; The component of described slant medium II: yeast powder 10 grams, glucose 10, dipotassium hydrogen phosphate 0.5 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH5.5.The concrete operations that in step (1), Rhodopseudomonas palustris activates in inclined-plane are: be inoculated on sterilized slant medium III with transfering loop by Rhodopseudomonas palustris, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 15min; And the component of this slant medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH 7.0-7.2.The concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in step (1) are: be inoculated on sterilized slant medium IV with transfering loop by yeast saccharomyces cerevisiae, aerobic cultivation 3 days at 28-30 DEG C afterwards; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, pH nature.
The component of liquid nutrient medium I in step (2): peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, 0.2 gram, magnesium sulfate, manganous sulfate 0.05 gram, 20 grams, calcium carbonate, water 1000ml, pH6.8; The component of liquid nutrient medium II: yeast powder 10 grams, glucose 10, dipotassium hydrogen phosphate 0.5 gram, 0.5 gram, magnesium sulfate, agar 2%, water 1000ml, pH5.5; The component of liquid nutrient medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH 7.0-7.2; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, pH nature.
The component of fermention medium I in step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium II in step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium III in step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
It should be noted that, in the present invention, the per-cent of each substratum is mass percent.
Compound micro-ecological preparation of the present invention is used for preventing epidemic to live pig, and applicant is applied to pig breeding farm for this compound micro-ecological preparation and carries out elaboration explanation, particularly:
Test group and blank group are set simultaneously: in test group, cultivating live pig 67, compound micro-ecological preparation the present invention prepared and water are mixed with water diluent according to the volume ratio of 1:100, this water diluent is sprayed on equably the surrounding of plant and live pig column home, and every square metre of each spray water diluent 0.1 kilogram; Start to spray 2-3 time weekly, spray weekly 1 time after one month, then progressively extend to 10 days once, monthly 1-2 time.Blank group does not spray the diluent of compound micro-ecological preparation.Experimental result is: test group live pig is all normally raised and delivers for sale, and namely mortality is 0%; Two live pig is had due to illness to exit experiment in blank group in feeding process, mortality 5.9%; And test group live pig whole process treats 36 times, treatment rate is 56.25%; Blank group live pig whole process treats 30 times, treatment rate 88.24%.Therefore, from experimental result, the epidemic prevention successful of test group.
To sum up, adopt compound micro-ecological preparation of the present invention can form a microenvironment based on Benign microbes at plant's periphery, obstruct pathogen transmission can be played, thus improve the effect of pig-breeding resistance against diseases, also have easy to use, act on obvious feature; And this compound micro-ecological preparation also has the advantage that technique is comparatively simple, cost is low, thus compound micro-ecological preparation of the present invention can be applied comparatively widely.
Claims (5)
1. for a compound micro-ecological preparation for live pig auxiliary epidemic prevention, it is characterized in that: the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Lactobacterium acidophilum in freezing pipe, bacillus aceticus, Rhodopseudomonas palustris, yeast saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain Lactobacterium acidophilum slant strains, bacillus aceticus slant strains, Rhodopseudomonas palustris slant strains and yeast saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: Lactobacterium acidophilum slant strains be inoculated in sterilized liquid nutrient medium I, be placed on 37 DEG C at Anaerobic culturel 1-2 days obtain Lactobacterium acidophilum original bacteria liquid; Bacillus aceticus slant strains be inoculated in sterilized liquid nutrient medium II, then at 30 DEG C, aerobic cultivation obtains bacillus aceticus original bacteria liquid in 1-2 days; Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium III, then within illumination cultivation 5-7 days, obtains Rhodopseudomonas palustris original bacteria liquid in 28 DEG C-30 DEG C anaerobism tengsten lamps; Yeast saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium IV, at 28 DEG C-30 DEG C, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: Lactobacterium acidophilum original bacteria liquid, bacillus aceticus original bacteria liquid, Rhodopseudomonas palustris original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermention medium I by the bacterium amount ratio of 1:1:1:1 and cultivate, particularly: first inoculate bacillus aceticus original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days at 30 DEG C, inoculate Lactobacterium acidophilum original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, at 30 DEG C, Anaerobic culturel 3 days, obtains the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum size be inoculated in sterilized fermention medium II, and at 30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum size be seeded in sterilized fermention medium III, and at 30 DEG C first aerobic 3-5 days Anaerobic culturel 3-5 days again, obtain the finished product of compound micro-ecological preparation;
Wherein, the component of described liquid nutrient medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, 0.2 gram, magnesium sulfate, manganous sulfate 0.05 gram, 20 grams, calcium carbonate, 15 grams, agar, water 1000ml, pH6.8; The component of described liquid nutrient medium II: yeast powder 10 grams, glucose 10, dipotassium hydrogen phosphate 0.5 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH5.5; The component of described liquid nutrient medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH 7.0-7.2; The component of described liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, pH nature; The component of described fermention medium I: tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, excess water; The component of described fermention medium II: tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water; The component of described fermention medium III: tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
2. a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention according to claim 1, it is characterized in that: the concrete operations that in described step (1), Lactobacterium acidophilum activates in inclined-plane are: be inoculated on sterilized slant medium I with transfering loop by Lactobacterium acidophilum, afterwards Anaerobic culturel 1-2 days at 37 DEG C; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, 0.2 gram, magnesium sulfate, manganous sulfate 0.05 gram, 20 grams, calcium carbonate, 15 grams, agar, water 1000ml, pH6.8.
3. a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention according to claim 1, it is characterized in that: the concrete operations that in described step (1), bacillus aceticus activates in inclined-plane are: prepare slant medium II, and sterilizing 20min at slant medium II is placed in 121 DEG C, then be cooled to less than 60 DEG C and add dehydrated alcohol, and every 1000ml slant medium II need add 100ml dehydrated alcohol, with transfering loop, bacillus aceticus is inoculated on slant medium II afterwards, then aerobic cultivation 1-2 days at 30 DEG C; The component of described slant medium II: yeast powder 10 grams, glucose 10 grams, dipotassium hydrogen phosphate 0.5 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH5.5.
4. a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention according to claim 1, it is characterized in that: the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: be inoculated on sterilized slant medium III with transfering loop by Rhodopseudomonas palustris, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 15min; And the component of this slant medium III: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH 7.0-7.2.
5. a kind of compound micro-ecological preparation for live pig auxiliary epidemic prevention according to claim 1, it is characterized in that: the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: be inoculated on sterilized slant medium IV with transfering loop by yeast saccharomyces cerevisiae, aerobic cultivation 3 days at 28-30 DEG C afterwards; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, pH nature.
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150909 |