CN1239669A - Composition of Radix Sophorae Flavescentis and green tea and its application in treating condyloma - Google Patents
Composition of Radix Sophorae Flavescentis and green tea and its application in treating condyloma Download PDFInfo
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- CN1239669A CN1239669A CN 99103108 CN99103108A CN1239669A CN 1239669 A CN1239669 A CN 1239669A CN 99103108 CN99103108 CN 99103108 CN 99103108 A CN99103108 A CN 99103108A CN 1239669 A CN1239669 A CN 1239669A
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- green tea
- crude extract
- radix sophorae
- sophorae flavescentis
- condyloma
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- 235000009569 green tea Nutrition 0.000 title claims description 19
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Abstract
The present invention relates to one kind of composition for treating sharp condyloma caused by papillary virus. Coarse Radix Sophorae Flavescentis extract is first neutralized completely with hydrochloric acid of proper concentration and then mixed with well-mixed coarse green tea extract to obtain brown ointment. Two kinds of coarse extract are tested through color spot reaction of specific spreader and developer on silica gel G sheet. As one kind of new effective medicine for sharp condyloma, the composition is applied through skin administration and has high cure rate and effective rate and no negative reaction.
Description
The present invention relates to the composition of medicine of the condyloma acuminatum that a kind of treatment causes because of human nipple virus (HPV).
Condyloma acuminatum is can detected a kind of wart on human body skin or men and women's genitals mucosa, and (HPV) causes by human nipple virus.This wart shows as distinguished mamillary or cockscomb sample tumor, and accumulation and multiple tendency are arranged.The sickness rate height of condyloma acuminatum, patient's number is in continuous increase.The main method of the condyloma acuminatum that treatment at present causes because of the human papillomavirus has that excision, electricity iron, freezing, laser therapy etc.Drug therapy has Pu Dafeilun fat, five-fluorouracil, podophyllotoxin etc.Yet said method all exists certain limitation and poison to pay effect at present, and therefore, therapeutic effect is also unsatisfactory.
The present invention's purpose is to seek a kind of new medicine, compares with present clinical application, and not only better efficacy, and toxic and side effects is very low, and life-time service can not cause harmful effect to the patient.
Content of the present invention and main points are: the effect that has heat-clearing and toxic substances removing, wind dispelling insecticide based on the Chinese medicine Radix Sophorae Flavescentis, be used for skin ulcer, scabies, tinea disease more, it is gynopathic medicine commonly used, green tea is the beverage that people use always, also have heat-clearing and toxic substances removing, effects such as convergence dehumidifying, and prove after deliberation, green tea has antiviral, antibiotic, antioxidation, anti-proliferative effect, so the effect of the combination treatment condyloma acuminatum of above-mentioned two kinds of extracts is furtherd investigate.
Concrete steps are as follows:
1, the preparation of Radix Sophorae Flavescentis crude extract:
The Chinese crude drug Radix Sophorae Flavescentis powder is broken into coarse powder, weighs, added 0.2%HCl moistening 30 minutes, the percolation bucket of packing into compresses, and slowly the impouring aforesaid liquid flooded 1 hour to not having the medicated powder interface, the beginning percolation, and flow velocity is 800-1000ml/cm
2/ hour, when percolate does not have obvious salmon pink precipitation (a little percolate adds one of Dragendorff's reagent), percolation finishes.Static 24 hours of percolate, the siphon supernatant, by the strong acid type cationic resin post, flow velocity is 500-800ml/cm
2/ hour, when appearring in exchange liquid, the salmon pink precipitation stops exchange.Inclining resin, clean with water rinse, and centrifuge dripping is dried resin below 40 ℃, adds an amount of strong aqua ammonia alkalization, in the apparatus,Soxhlet's of packing into, adds the chloroform reflux, extract,, colourless to chloroform solution.Inclining chloroform solution, filters, and decompression and solvent recovery, concentrated solution add small amount of ethanol and dissolve, and reclaim under reduced pressure gets the Radix Sophorae Flavescentis crude extract to there not being the alcohol flavor.
The pretreatment and the regeneration step of strong acid type cationic resin are as follows: resin is used warm water soaking 2 hours, and the dress post flooded 10 hours with 5-7%HCl, and the above-mentioned hydrochloric acid that reuse 3-5 doubly measures makes the transition, and flow velocity is 500-800ml/cm
2/ hour, be washed till PH6-7 with distilled water then and get final product.(see figure 1)
2, the preparation of green tea crude extract:
Get the green tea end and weigh, the water logging that adds 80 ℃ of 10 times of amounts was steeped 2 hours, filter pressing, and residue adds the water loggings of 80 ℃ of 5 times of amounts again and steeped 1 hour, and filter pressing discards residue, merging filtrate.Add one times of amount chloroform extraction in the filtrate 30 minutes, and left standstill, discard chloroform layer, divide water outlet liquid, added one times of amount ethyl acetate extraction in the water liquid 30 minutes, leave standstill, collect acetic acid ethyl fluid, the same extraction again of water liquid once discards water liquid, combined ethyl acetate liquid, decompression and solvent recovery, concentrate adds the little water dissolving, and vacuum drying below 50 ℃ gets the green tea crude extract.(see figure 2)
3, Radix Sophorae Flavescentis crude extract and green tea crude extract preparation of compositions:
Get Radix Sophorae Flavescentis crude extract 14 gram, add 8N hydrochloric acid 5-8ml, fully stir, make neutralization fully, to avoid and the chemical action of green tea crude extract (this effect can cause compositions to form new chemical compound and lose therapeutical effect).Get Yellow Vaselin 40 grams, anhydrous lanolin 20 grams, white oil 5 grams, 70 ℃ of water-bath fusings, add 10 gram green tea crude extracts (crossing 100 mesh sieves), stir, slowly add above-mentioned Radix Sophorae Flavescentis crude extract, the limit edged stirs, after treating fully evenly, stop heating, continue to be stirred to room temperature, the packing (see figure 3).
The said Radix Sophorae Flavescentis of the present invention, green tea compositions also can be made into suppository or other dosage form, to satisfy clinical different needs.
4, the detection of compositions and discriminating:
Get compositions ointment 1 gram, add dehydrated alcohol 5ml, 3 of enriching ammonia, heating in water bath is put to room temperature to fusing, filters, and draws filtrate 20 μ l points on the silica gel g thin-layer plate with the 2%NaOH formulations prepared from solutions; Other gets the matrine reference substance, is made into the ethanol solution that every 1ml contains 0.1mg, draws 10 μ l points on same silica gel g thin-layer plate.
Developing solvent: toluene: acetone: ethanol: strong aqua ammonia
(20∶20∶3∶1)
Developer: improvement Dragendorff's reagent liquid
Conclusion: in the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show identical orange red speckle (accompanying drawing 5)
Get compositions ointment 1 gram, add dehydrated alcohol 5ml, heating in water bath is put to room temperature to fusing, filters, and draws filtrate 20-40 μ l, puts on silica gel g thin-layer plate; Other gets green tea reference substance 2 grams, adds the 10ml soaked in absolute ethyl alcohol 2 hours, filters, and draws filtrate 20-40 μ l, puts on same silica gel g thin-layer plate.
Developing solvent: chloroform: methanol: formic acid (8: 20: 0.1)
Developer: put and observe under the uviol lamp or room temperature placement speckle to be showed.
Conclusion: in the test sample chromatograph, with reference substance chromatograph relevant position on, show identical aubergine speckle (Fig. 4).
5, the animal irritation test of compositions and clinical efficacy are judged:
In view of compositions involved in the present invention is to be made of Chinese crude drug Radix Sophorae Flavescentis commonly used and two kinds of extracts of beverage green tea, so except that irritation test adopts animal model, can observe the effect of its treatment condyloma acuminatum directly by clinical trial.
1) mucous membrane of animal irritation test;
Medicine: condyloma latum spirit; Reference substance: blank substrate.
Animal: 4 of rabbit, body weight is 1800-2000g, purchases the animal center in Nat'l Pharmaceutical ﹠ Biological Products Control Institute.
Medication and dosage:
Be coated with an amount of ointment in the rabbit eyelid with disinfecting cotton swab.1st, the right eye of 2,3,4 rabbit all is coated with the condyloma latum spirit, and its left eye all is coated with blank substrate and compares.
Observe before the administration:
Every rabbit eyes are all normal, and conjunctive bulbi does not have hyperemia, and the corneal light reflection is normal, and eyelid opens and closes normal, and diet and activity are all normal.
Observe after the administration: (administration for the first time)
Observe after 1 hour:
1st, 2,3,4 rabbit right eyes close more; Left eye is all normal.Nonsystemic reaction.
Observe after 3 hours
1st, 2,3,4 still closing of rabbit right eye more, the 2nd, 4 rabbit right conjunctive bulbi is rubescent slightly; Left eye is normal, nonsystemic reaction.
Observe after 6 hours:
4 rabbit right eyes before take a favorable turn.Left eye is normal, nonsystemic reaction.
Observe after 20 hours
The right eye of 4 rabbit all recovers normally, whole body movable normal (table 1)
Observe after table 1 administration for the first time
*Refer to conjunctive bulbi
| The rabbit ordinal number time | The 1st | The 2nd | The 3rd | The 4th | Remarks | ||||
| Left eye | Right eye | Left eye | Right eye | Left eye | Right eye | Left eye | Right eye | ||
| After 1 hour | Normally | Close more | Normally | Close more | Normally | Close more | Normally | Close more | Nonsystemic reaction |
| After 3 hours | Normally | Close more | Normally | To close * rubescent slightly more | Normally | Close more | Normally | To close * rubescent slightly more | Nonsystemic reaction |
| After 6 hours | Normally | Take a favorable turn | Normally | Take a favorable turn | Normally | Take a favorable turn | Normally | Take a favorable turn | Nonsystemic reaction |
| After 20 hours | Normally | Normally | Normally | Normally | Normally | Normally | Normally | Normally | Normally |
Observe (administration for the second time after 7 days) after the administration
Observe after 1 hour:
1st, 2 rabbit right conjunctive bulbis are rubescent slightly, and eye closes more; 3rd, 4 rabbit right eyes are more normal.Left eye is all normal, nonsystemic reaction.
Observe after 3 hours
The 1st rabbit right conjunctive bulbi is rubescent slightly, and eye closes more, and the 2nd rabbit right eye take a favorable turn still closes the 3rd, 4 rabbit right emmetropia more.Left eye is all normal, nonsystemic reaction.
Observe after 6 hours
The 1st rabbit right eye take a favorable turn, and the 2nd rabbit right eye is normal substantially, the 3rd, 4 rabbit right emmetropia.Left eye is all normal, nonsystemic reaction.
Observe after 20 hours:
1st, 2,3,4 rabbit right eyes all recover normally, and left eye is all normal, whole body movable normal (table 2).
Observe after table 2 administration for the second time (administration for the first time is after 7 days)
*Refer to the conjunctive bulbi conclusion: condyloma latum spirit nonirritant.2) clinical observation on the therapeutic effect; Treatment target is condyloma acuminatum patient 49 examples of making a definite diagnosis; Route of administration is external, is coated on patient part; Dosage is grasped according to affected part area and degree adjustment.The treatment standard is divided into healing (condyloma latum all disappears); Produce effects (condyloma latum disappearance rate＞50%); Invalid (condyloma latum disappearance rate＜50%), the course of treatment, average out to was about one month.
| The rabbit ordinal number time | The 1st | The 2nd | The 3rd | The 4th | Remarks | ||||
| Left eye | Right eye | Left eye | Right eye | Left eye | Right eye | Left eye | Right eye | ||
| After 1 hour | Normally | To close * rubescent slightly more | To close * rubescent slightly more | Normally | More normal | Normally | More normal | Nonsystemic reaction | |
| After 3 hours | Normally | To close * rubescent slightly more | Normally | Close more | Normally | More normal | Normally | More normal | Nonsystemic reaction |
| After 6 hours | Normally | Take a favorable turn | Normally | Substantially normal | Normally | Normally | Normally | Normally | Nonsystemic reaction |
| After 20 hours | Normally | Normally | Normally | Normally | Normally | Normally | Normally | Normally | Normally |
Through the clinic trial result, total effective rate reaches 90% (table 3):
| The example number | Standard | The course of treatment | Ratio | Side effect | |
| Cure | ????37 | All disappear | 35 days ± | 76％ | Indivedual case idols have gargalesthesia |
| Produce effects | ????7 | ???＞50％ | 30 days ± | 14％ | ?????、、 |
| Invalid | ????5 | ???＜50％ | 20 days ± | 10％ | ?????、、 |
According to document announcement, use green tea extract (tea polyphenols) treatment condyloma acuminatum separately, its cure rate is 54%, obvious effective rate is 32%, total effective rate is 86%, show that Radix Sophorae Flavescentis and green tea extract are through the special handling composition prepared, therapeutic effect obviously is better than using separately the green tea extract tea polyphenols.
Advantage of the present invention and effect are, compare with the medicine of present clinical use treatment condyloma acuminatum, this compositions (condyloma latum spirit) good effect, non-stimulated, there is not allergy, have no side effect, but life-time service, and easy and simple to handle, the patient can be about to medicine certainly and be applied to the affected part, so a kind of safe and reliable active drug of can yet be regarded as.
Description of drawings:
Fig. 1: Radix Sophorae Flavescentis crude extract preparation flow figure.
Fig. 2: green tea crude extract preparation flow figure.
Fig. 3: Radix Sophorae Flavescentis, green tea crude extract preparation of compositions flow chart.
Fig. 4: the thin layer of green tea extract is differentiated collection of illustrative plates
Wherein: 1---the condyloma latum spirit
2---the green tea extract reference substance
Fig. 5: the thin layer of Radix Sophorae Flavescentis extract is differentiated collection of illustrative plates
Wherein: 1---the Radix Sophorae Flavescentis crude extract
2,3---the condyloma latum spirit
4---the matrine reference substance
Claims (4)
1, a kind of condyloma latum spirit and manufacture method thereof is characterized in that said composition system is formulated and have an effect for the treatment of condyloma acuminatum preferably through special handling by Radix Sophorae Flavescentis crude extract and green tea crude extract.
2,, it is characterized in that basic process steps is according to described condyloma latum spirit of claim 1 and manufacture method thereof:
A) the Radix Sophorae Flavescentis crude extract is stirred evenly to neutralization fully with 8N hydrochloric acid earlier, place standby;
B) Yellow Vaselin, anhydrous lanolin and white oil (70 ℃ of water-bath fusings) are added the green tea crude extract;
C) Radix Sophorae Flavescentis crude extract that pretreatment is good mixes with B under proper temperature and stirs evenly, sepia ointment.
3, according to claim 1 or 2 described condyloma latum spirit and manufacture methods thereof, the detection judging standard that it is characterized in that resulting composition is: Radix Sophorae Flavescentis crude extract and matrine reference substance are on same silica gel g thin-layer plate, after specific developing solvent and developer processing, its chromatograph relevant position should show identical orange red speckle; Green tea crude extract and green tea reference substance are on same silica gel g thin-layer plate, and after specific developing solvent and developer processing, its chromatograph relevant position should show identical aubergine speckle.
4, according to described condyloma latum spirit of claim 1 and manufacture method thereof, it is characterized in that the clinical treatment condyloma acuminatum,, grasp dosage according to pathological changes area and degree through skin topical administration approach, average course of treatment be 30 days ±, according to healing, produce effects, invalid and side effect standard determination curative effect and safety.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99103108A CN1093408C (en) | 1999-03-24 | 1999-03-24 | Composition of Radix Sophorae Flavescentis and green tea and its application in treating condyloma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99103108A CN1093408C (en) | 1999-03-24 | 1999-03-24 | Composition of Radix Sophorae Flavescentis and green tea and its application in treating condyloma |
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| Publication Number | Publication Date |
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| CN1239669A true CN1239669A (en) | 1999-12-29 |
| CN1093408C CN1093408C (en) | 2002-10-30 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425986C (en) * | 2004-08-27 | 2008-10-15 | 广州中一药业有限公司 | Quality control method for Wushezhiyang pill |
| CN102636585A (en) * | 2012-03-29 | 2012-08-15 | 武汉华大药业有限公司 | Method for detecting a compound preparation of green tea of radix sophorae flavescentis |
| CN102641386A (en) * | 2012-03-29 | 2012-08-22 | 武汉华大药业有限公司 | Vaginal effervescent tablet prepared from radix sophorae flavescentis and green tea and preparation method thereof |
| CN102652778A (en) * | 2012-03-29 | 2012-09-05 | 武汉华大药业有限公司 | Medicinal composition |
| CN102652777A (en) * | 2012-03-29 | 2012-09-05 | 武汉华大药业有限公司 | Kuh-seng and green tea suppository and preparation method thereof |
| CN102652730A (en) * | 2012-03-29 | 2012-09-05 | 武汉华大药业有限公司 | Ointment base, kuh-seng and green tea ointment and preparation method of ointment |
| CN102824448A (en) * | 2011-06-15 | 2012-12-19 | 福建明龙制药有限公司 | Drug composition for preventing and/or treating gynecological diseases |
| CN103110746A (en) * | 2013-02-15 | 2013-05-22 | 天津中医药大学 | Effective antibacterial traditional chinese medicine composition |
| CN103202920A (en) * | 2013-02-15 | 2013-07-17 | 天津中医药大学 | Traditional Chinese medicine composition with antibacterial function |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066047C (en) * | 1996-12-25 | 2001-05-23 | 藏镜浩 | Traditional Chinese medicine preparation for treatment of sharp-pointed condyloma |
| CN1070376C (en) * | 1997-12-26 | 2001-09-05 | 穆永森 | External-use medicinal liquor for treatment of acute moist wart and preparation method therefor |
-
1999
- 1999-03-24 CN CN99103108A patent/CN1093408C/en not_active Expired - Lifetime
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425986C (en) * | 2004-08-27 | 2008-10-15 | 广州中一药业有限公司 | Quality control method for Wushezhiyang pill |
| CN102824448A (en) * | 2011-06-15 | 2012-12-19 | 福建明龙制药有限公司 | Drug composition for preventing and/or treating gynecological diseases |
| CN102824448B (en) * | 2011-06-15 | 2016-08-10 | 福建明龙制药有限公司 | A kind of prevention and/or the pharmaceutical composition for the treatment of gynaecopathia |
| CN102652730B (en) * | 2012-03-29 | 2014-04-16 | 武汉华大药业有限公司 | Ointment base, kuh-seng and green tea ointment and preparation method of ointment |
| CN102652777A (en) * | 2012-03-29 | 2012-09-05 | 武汉华大药业有限公司 | Kuh-seng and green tea suppository and preparation method thereof |
| CN102652730A (en) * | 2012-03-29 | 2012-09-05 | 武汉华大药业有限公司 | Ointment base, kuh-seng and green tea ointment and preparation method of ointment |
| CN102652778A (en) * | 2012-03-29 | 2012-09-05 | 武汉华大药业有限公司 | Medicinal composition |
| WO2013143412A1 (en) * | 2012-03-29 | 2013-10-03 | 武汉华大药业有限公司 | Pharmaceutical compositions |
| CN102641386B (en) * | 2012-03-29 | 2014-02-19 | 武汉华大药业有限公司 | Vaginal effervescent tablet prepared from radix sophorae flavescentis and green tea and preparation method thereof |
| CN102636585B (en) * | 2012-03-29 | 2014-02-19 | 武汉华大药业有限公司 | Method for detecting a compound preparation of green tea of radix sophorae flavescentis |
| CN102641386A (en) * | 2012-03-29 | 2012-08-22 | 武汉华大药业有限公司 | Vaginal effervescent tablet prepared from radix sophorae flavescentis and green tea and preparation method thereof |
| CN102636585A (en) * | 2012-03-29 | 2012-08-15 | 武汉华大药业有限公司 | Method for detecting a compound preparation of green tea of radix sophorae flavescentis |
| CN103110746A (en) * | 2013-02-15 | 2013-05-22 | 天津中医药大学 | Effective antibacterial traditional chinese medicine composition |
| CN103202920A (en) * | 2013-02-15 | 2013-07-17 | 天津中医药大学 | Traditional Chinese medicine composition with antibacterial function |
| CN103110746B (en) * | 2013-02-15 | 2017-05-03 | 天津中医药大学 | Effective antibacterial traditional chinese medicine composition |
| CN103202920B (en) * | 2013-02-15 | 2017-11-24 | 天津中医药大学 | Chinese medicine composition with antibacterial action |
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