CN109125703A - A kind of pharmaceutical composition and preparation method thereof for treating vaginitis - Google Patents
A kind of pharmaceutical composition and preparation method thereof for treating vaginitis Download PDFInfo
- Publication number
- CN109125703A CN109125703A CN201811203980.XA CN201811203980A CN109125703A CN 109125703 A CN109125703 A CN 109125703A CN 201811203980 A CN201811203980 A CN 201811203980A CN 109125703 A CN109125703 A CN 109125703A
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- moringa
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- pharmaceutical composition
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- 206010046914 Vaginal infection Diseases 0.000 title claims abstract description 44
- 201000008100 Vaginitis Diseases 0.000 title claims abstract description 38
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000012530 fluid Substances 0.000 claims abstract description 60
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 44
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- 235000011347 Moringa oleifera Nutrition 0.000 claims abstract description 39
- 238000000605 extraction Methods 0.000 claims abstract description 39
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- 238000000034 method Methods 0.000 claims abstract description 38
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- 229920001184 polypeptide Polymers 0.000 claims abstract description 24
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Classifications
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Abstract
The invention belongs to pharmaceutical technology field, specifically a kind of pharmaceutical composition and preparation method thereof for treating vaginitis.The pharmaceutical composition raw material each group synthetic ingredient and its weight fraction ratio are as follows: rape flower -15 ~ 20 parts of Moringa polypeptide, 20 ~ 25 parts of frutus cnidii, 20 ~ 25 parts of Cortex Phellodendri, 20 ~ 25 parts of the fruit of summer cypress, 20 ~ 25 parts of rhizoma cyperi, 20 ~ 25 parts of kuh-seng, 20 ~ 25 parts of sargentodoxa cuneata, 10 ~ 20 parts of curcuma zedoary, 10 ~ 20 parts of field pennycress, 18 ~ 20 parts of honeysuckle, 12 ~ 15 parts of dried orange peel, 12 ~ 15 parts of the leaf of bamboo, 8 ~ 12 parts of Chinese lantern, 10 ~ 12 parts of hypericum japonicum, 8 ~ 10 parts of peppermint；Preparation method be first using reflux extraction, ultrasonic vibrating method, percolation, decocting method extract medical fluid, reuse chitin modified polymer and gelatin and medical fluid further purified, the medical fluid after purification through antibacterial, preservative treatment to obtain the final product.
Description
Technical field
The invention belongs to pharmaceutical technology field, specifically a kind of pharmaceutical composition and its preparation side for treating vaginitis
Method.
Background technique
Vagina is the channel of menstruation and leukorrhea, is the pathway of childbirth and the main place of sexual intercourse, is female body structure
One of indispensable part is made, its integrality and normal function should be kept.Vaginitis is women common disease, frequently-occurring disease, with
Pruritus vulvue, secretion increase etc. as main clinical manifestation, due to the particularity of female external genital organs device, pole in any case
Vulnerable to some pathogenic bacteria.Infringement, it is strong to the body and mind of women wherein have a considerable amount of patient's obstinates or recurrent exerbation
Health makes a big impact.To generally there are 3 times or 3 times or more breaking-outs to be known as recurrent vaginal in vaginitis patient 1 year scorching.Normal
In the case of vaginal fluid be limpid, transparent and free from extraneous odour, will not cause that vulva is irritating and the feeling of itch, general yin
The clinical manifestation of road inflammation is leucorrhea increasing, is in yellow, yellow green purulence or bean dregs sample, and thin or hemorrhagic secretion has stench
Taste, vulva, pruritus of vagina and burn feeling are likely to occur frequent micturition, urodynia, intercourse pain or dyspareunia when serious, nymphae is glutinous
Film and vaginal mucosa hyperemia, red and swollen, rotten to the corn, ulcer and tenderness etc..Common vaginitis has bacterial vaginitis, fungoid yin
Road inflammation, trichomonas vaginitis, senile vahinitis and infantile vaginitis.
In recent years, the drug of many treatment vaginitis has been studied both at home and abroad, and the treatment of vaginitis, which has, further to be changed
Into and improve.Antibiotic or western medicine are largely used in the past, and toxic side effect is big, is more to combine Chinese medicine and Chinese and Western now
It cures the method combined and carries out treatment vaginitis, have certain effect in treatment vaginitis, but existing Traditional chinese medicine medicament is deposited
Complicated preparing, curative effect is indefinite, and treatment cycle is longer, easily recurs after treatment, fails to reach good therapeutic effect.
Summary of the invention
The present invention provides a kind of pharmaceutical composition and preparation method thereof for treating vaginitis, which carries out science
Property compatibility, colpitis can be effectively prevented and treated by the interaction of various effective components, it is multiple that colpitis is effectively reduced
Hair rate, therapeutic effect is good, and toxic side effect is small.
The scheme of the invention is realize in this way: a kind of for treating the pharmaceutical composition of vaginitis, the pharmaceutical composition
Raw material each group synthetic ingredient and its weight fraction ratio are as follows: rape flower -15 ~ 20 parts of Moringa polypeptide, 20 ~ 25 parts of frutus cnidii, Cortex Phellodendri 20 ~
25 parts, 20 ~ 25 parts of the fruit of summer cypress, 20 ~ 25 parts of rhizoma cyperi, 20 ~ 25 parts of kuh-seng, 20 ~ 25 parts of sargentodoxa cuneata, 10 ~ 20 parts of curcuma zedoary, field pennycress 10
~ 20 parts, 18 ~ 20 parts of honeysuckle, 12 ~ 15 parts of dried orange peel, 12 ~ 15 parts of the leaf of bamboo, 8 ~ 12 parts of Chinese lantern, 10 ~ 12 parts of hypericum japonicum, peppermint 8 ~
10 parts.
As a further improvement of the present invention, the rape flower-Moringa polypeptide the preparation method comprises the following steps:
A. it is dried after taking the leaf of Moringa of fresh no mildew to be cleaned with clear water, the leaf of Moringa dried is then ground into 20 ~ 30 purposes
Moringa powder；It is dried after taking fresh rape flower wash clean, the rape flower after drying is ground into the rape of 10 ~ 20 mesh
Pollen；
B. it is that 2:1 takes moringa powder and rape pollen to be mixed respectively according to mass ratio, pure water is added after mixing and makes
Solid-liquid ratio is 1:5.0 ~ 1:10.0, and after adjusting material liquid pH value is 9.0 and then feed liquid is heated to 50 ~ 60 DEG C again, alkaline egg is added
White enzyme stirs, and carries out 20 ~ 30min of enzymatic hydrolysis, is heated to 100 DEG C of progress destroy the enzyme treatments, then again digests first time
Liquid pH value be adjusted to 6.0 ~ 7.5 after according to substrate solution weight 2% be added neutral proteinase, according to the 2% of substrate solution weight
Trypsase is added, papain is added according to the 3% of substrate solution weight, then is passed through high-pressure pulse electric, in high-voltage pulse
Start microwave under conditions of electric field is normally opened to be handled, the pulse number that high-pressure pulse electric is arranged is 6 ~ 8, and electric field strength is
10~20Kv.cm-1, setting microwave heating temperature be 50 ~ 55 DEG C, microwave power be 400 ~ 800W handle the time be 20 ~ 30min, enzyme
Electromagnetic field is closed after solution, microwave is again started up by temperature and rises to 95 ~ 100 DEG C, and 8 ~ 10min is maintained to carry out destroy the enzyme treatment, it is cold
But Peptides liquid is obtained to room temperature；
C. 8 ~ 10min is centrifuged with the revolving speed of 4000 ~ 6000r/min in Peptides liquid, then takes supernatant 50 ~ 60
It DEG C is concentrated in vacuo, carries out being freeze-dried up to rape flower-Moringa polypeptide at -60 ~ -50 DEG C.
A method of preparing the pharmaceutical composition for treating vaginitis, comprising the following steps:
(1) preparation of chitin modified polymer flocculants: the chitosan by deacetylation higher than 90% is washed, is air-dried, constant weight
Afterwards, which is dissolved in the acetum that concentration is 0.1mol/L, magnetic agitation is made into the chitosan that mass concentration is 2%
Acetum；It takes the 150mL solution to be fitted into the three-necked flask with thermometer and stirring rod, citric acid solid is added, lead to nitrogen
It is started to warm up after stirring to dissolve to 65 ~ 75 °C, ammonium ceric nitrate 0.2g is added three times in 30min, is continued in nitrogen atmosphere
Magnetic agitation reacts 3h, stops logical nitrogen, is cooled to room temperature to reaction system and obtains thick graft product, settled using acetone, second
Alcohol extracts 2 ~ 3h, and vacuum is dried overnight, up to chitin modified polymer flocculants after grinding；
(2) separating out alcohol method extracts: taking 20 ~ 25 parts of frutus cnidii, 20 ~ 25 parts of Cortex Phellodendri, 20 ~ 25 parts of the fruit of summer cypress, 20 ~ 25 parts of rhizoma cyperi, kuh-seng
20 ~ 25 parts, 10 ~ 20 parts of curcuma zedoary, 20 ~ 35 parts of sargentodoxa cuneata, 10 ~ 20 parts of field pennycress mixing after carry out the mixed powder for being ground into 20 mesh
End, ethyl alcohol of the addition relative to 4 ~ 5 times of mixed-powder quality of 80% ~ 85%v/v, heating and refluxing extraction 1 ~ 2 hour, refluxing extraction
Twice, ultrasonic vibrating 30min is used after reflux every time, the solution that refluxing extraction obtains respectively twice is merged
To mixing phegma；The remaining filter residue powder of refluxing extraction is extracted using percolation, does solvent, dipping 24 with the ethyl alcohol of 50%v/v
Hour, with the speed of 2 ~ 3ml per minute slowly diacolation, collect the percolates of 5 times of amounts；After mixing phegma is merged with percolate
It is filtered using vacuum pump nutsch filter, medical fluid A is obtained after filtering, storage is spare, and filter paper specification used in vacuum pump nutsch filter is
Middling speed quantitative filter paper, aperture are 30 ~ 50 microns；
(3) decocting method extracts: 18 ~ 20 parts of honeysuckle, 12 ~ 15 parts of dried orange peel, 12 ~ 15 parts of the leaf of bamboo, 8 ~ 12 parts of Chinese lantern, hypericum japonicum 10
~ 12 parts, 8 ~ 10 parts of peppermint mixing after be ground into 10 mesh mixed-powders, then press 2 ~ 3 times of addition distilled water of mixed-powder total weight,
Warm fire decocts 2 ~ 3 hours, uses ultrasonic vibrating 30min after decocting every time, decocts and ultrasonic vibrating merges afterwards twice
Extracting solution twice is filtered extracting solution twice using vacuum pump nutsch filter, and medical liquid B is obtained after filtering, stores spare；
(4) flocculence purifies: merging medical liquid B obtained by medical fluid A obtained by step 1) and step 2), is adjusted after merging with dilute hydrochloric acid solution
Medical fluid pH value to 5 ~ 7, fluid temperature is risen to 50 ~ 70 DEG C, is 60 ~ 80r/min stirring with speed, heating stirring while adds
Enter to account for the chitin modified polymer flocculants and account for the gelatin that medical fluid weight is 0.05% that medical fluid weight is 0.1%, then is mentioned
Pure 2h is filtered after purification using vacuum pump nutsch filter, and rape flower -15 ~ 20 parts of Moringa polypeptide, nipalgin is added in filtered medical fluid
It 5 ~ 6 parts of ester, is mixed evenly up to the pharmaceutical composition for treating vaginitis.
As a further improvement of the present invention, the pharmaceutical composition can be made into lotion, spraying according to conventional dose technique
Agent, cream, suppository, tablet.
As a further improvement of the present invention, the paraben esters are one of ethylparaben, propylben
Or two kinds of combination.
The technical principle that the present invention realizes is:
The present invention can using integrated processes such as enzymatic hydrolysis, electric field and ultrasonic waves to rape flower, leaf of Moringa progress peptides extraction
Peptide constituents in extraction rape flower, peptides, while keeping the activity of peptide constituents.The rape flower of extraction-Moringa peptide, which has, to disappear
Inflammation, antibacterial, the effect for improving immunity of organisms, rape flower-Moringa peptide is added in the pharmaceutical composition for being used to treat vaginitis
Treatment colpitis medicine antibacterial and anti-inflammation functions can be mentioned, are improved the immunity of the human body simultaneously.The frutus cnidii of addition, Cortex Phellodendri, the fruit of summer cypress,
The Chinese herbal medicines such as rhizoma cyperi, kuh-seng have clearing heat and detoxicating, dispelling wind and eliminating dampness, and desinsection is antipruritic and other effects, can effectively treat by wet poison institute
The bacillary of cause, fungoid, trichomonas vaginitis and cutaneous pruritus.Curcuma zedoary, sargentodoxa cuneata, field pennycress have analgesic, dispersing blood stasis
And other effects, the pain sense due to caused by vaginitis can be improved, while also having effects that sterilizing and itch-relieving.Chinese medicine through the invention
The interaction of each ingredient of side, can effectively treat and prevent vaginitis, be conducive to vagina health.
Flocculant chitosan is modified, since chitosan side chain contains the functional group of amino, hydroxyl isoreactivity, easily
Macromolecular radical is generated on the strand of chitosan in progress chemical modification, therefore by initiator ammonium ceric nitrate, in turn
Cause vinyl monomer and sodium citrate carries out graft copolymerization.Sodium citrate as common stabilizer, pH adjusting agent and
Stabilizer has feature soluble easily in water, is used for the modification of chitosan to solve chitosan dissolubility is poor, molecular weight is low etc.
Problem, to improve impurity-eliminating effect of the chitosan as flocculant.Simultaneously with the chitin modified poly- of quality of liquid medicine score 0.1%
The gelatin combination purification of object and 0.05% is closed, the two mass fraction can be reinforced solidifying to tiny particles content than the best compatibility for 2:1
Collect effect, improves the purity of medical fluid.The selection of Chinese medicine preparation is wet according to vaginitis, the interpretation of the cause, onset and process of an illness feature such as hot and the course of disease are long, disease
The characteristics of feelings are obstinate, refractory, high recurrence rate, according to pharmacological effect organic assembling, the dialectical compatibility of medicinal material, dispels to reach heat-clearing
Wet, blood-activating analgetic effectively treats the purpose of vaginitis.
In preparation process, frutus cnidii, Cortex Phellodendri, the fruit of summer cypress, rhizoma cyperi, kuh-seng, curcuma zedoary, big blood are extracted using separating out alcohol method first
Effective component in rattan, field pennycress, wherein with alcohol solvent, the active constituent that can be effectively retained in medicinal material, and it is conducive to vagina pair
The absorption of drug.Specially reflux extraction extracts the volatile oil in medicinal material, is mentioned every time using ultrasonic vibrating after reflux
It takes, filter residue further uses percolation extraction, it is ensured that the effective component that can be sufficiently totally separated from out in medicinal material；Secondly it uses
Decocting method extracts the effective component in honeysuckle, dried orange peel, the leaf of bamboo, Chinese lantern, hypericum japonicum, peppermint, after decocting every time
It plus with ultrasonic vibrating extracts, to improve decocting method, time-consuming, ineffective in medical separation effective component extracting
Problem；Finally using flocculence to medical fluid carry out separating-purifying, the more traditional ultrafiltration of this method and enzyme process it is more simple and easy to do,
It is economical and practical, the paraben esters with anti-corrosion, antibacterial effect are added, stability when to very high medical fluid deposit.Medical fluid extracts
The rate of filtration is accelerated using the filtering of vacuum pump nutsch filter in the process, improves pharmacy efficiency.
The present invention has following good result:
1. the present invention follows the feature of vaginitis, feature, reasonable compatibility is carried out to medicinal material, the medical fluid arrived produced is compared with traditional decoction
The active constituent of method more drug effect remained in medicinal material, makes it play better therapeutic effect, and toxic side effect is small.
2. medicinal material mentioned by the present invention is Common Chinese Herbs, cheap, the burden of patient is alleviated.
3. the present invention carries out chemical modification to chitosan, its water solubility is enhanced, active group is introduced, adds its molecular weight
Greatly, flocculating property of the booster as medical fluid inorganic agent, the more unmodified more efficiently impurity eliminated in medical fluid before.
4. the made pharmaceutical composition Clinical Practice obtained of the present invention, the effective percentage for treating vaginitis is 94 ~ 96%,
It is counted after carrying out random access to patient after treatment, recurrence rate is 2 ~ 4%, and therapeutic effect is preferable.
Specific embodiment
A kind of pharmaceutical composition and preparation method thereof for treating vaginitis of the description present invention with reference to embodiments, these
Description is not to be further limited to the content of present invention.
Embodiment 1
The preparation of rape flower-Moringa polypeptide:
It is dried after taking the leaf of Moringa of fresh no mildew to be cleaned with clear water, then the leaf of Moringa dried is ground into the Moringa of 20 mesh
Powder；It is dried after taking fresh rape flower wash clean, the rape flower after drying is ground into the rape pollen of 10 mesh；
It is that 2:1 takes moringa powder and rape pollen to be mixed respectively according to mass ratio, pure water is added after mixing and to expect
Liquor ratio is 1:10.0, after adjusting material liquid pH value is 9.0 and then feed liquid is heated to 58 DEG C again, alkali protease is added and is sufficiently stirred
Uniformly, enzymatic hydrolysis 25min is carried out, 100 DEG C of progress destroy the enzyme treatments is heated to, first time enzymolysis liquid pH value is then adjusted to 7.0 again
Neutral proteinase is added according to the 2% of substrate solution weight afterwards, trypsase is added, the bottom of according to according to the 2% of substrate solution weight
Papain is added in the 3% of object solution weight, then is passed through high-pressure pulse electric, opens under conditions of high-pressure pulse electric is normally opened
Dynamic microwave is handled, and the pulse number that high-pressure pulse electric is arranged is 6, electric field strength 18Kv.cm-1, microwave is set and is added
Hot temperature is 52 DEG C, microwave power be 800W processing the time be 25min, close electromagnetic field after enzymatic hydrolysis, be again started up microwave
Temperature is risen to 98 DEG C, maintains 10min to carry out destroy the enzyme treatment, is cooled to room temperature to obtain Peptides liquid；
9.5min is centrifuged with the revolving speed of 4000r/min in Peptides liquid, then takes supernatant in 55 DEG C of progress vacuum
Concentration carries out being freeze-dried up to rape flower-Moringa polypeptide at -53 DEG C；
The preparation of chitin modified polymer flocculants: the chitosan by deacetylation higher than 90% is washed, is air-dried, after constant weight,
The chitosan is dissolved in the acetum that concentration is 0.1mol/L, magnetic agitation is made into the chitosan acetic acid that mass concentration is 2%
Solution；It takes the 150mL solution to be fitted into the three-necked flask with thermometer and stirring rod, citric acid solid is added, lead to nitrogen gas stirring
It is started to warm up after making it dissolve to 73 °C, ammonium ceric nitrate 0.2g is added three times in 30min, continues magnetic force in nitrogen atmosphere and stirs
Reaction 3h is mixed, stops logical nitrogen, is cooled to room temperature to reaction system and obtains thick graft product, settled using acetone, ethanolic extraction
3h, vacuum are dried overnight, up to chitin modified polymer flocculants after grinding；
Separating out alcohol method extract: take 21 parts of frutus cnidii, 23 parts of Cortex Phellodendri, 20 parts of the fruit of summer cypress, 22 parts of rhizoma cyperi, 24 parts of kuh-seng, 21 parts of sargentodoxa cuneata,
The mixed-powder for being ground into 20 mesh is carried out after 12 parts of curcuma zedoary, 13 parts of field pennycress mixing, is added relative to 4.5 times of mixed-powder quality
85%v/v ethyl alcohol, heating and refluxing extraction 2 hours, refluxing extraction twice, every time reflux after use ultrasonic vibrating
30min merges the solution that refluxing extraction obtains respectively twice to obtain mixing phegma；The remaining filter residue powder of refluxing extraction
It is extracted using percolation, makees solvent with the ethyl alcohol of 50%v/v, impregnate 24 hours, with the speed of 2.5ml per minute slowly diacolation,
Collect the percolate of 5 times of amounts；It is filtered after mixing phegma is merged with percolate using vacuum pump nutsch filter, medicine is obtained after filtering
Liquid A, storage is spare, and filter paper specification used in vacuum pump nutsch filter is middling speed quantitative filter paper, and aperture is 35 microns；
Decocting method extracts: 18 parts of extracting honeysuckle, 13 parts of dried orange peel, 12 parts of the leaf of bamboo, 9 parts of Chinese lantern, 12 parts of hypericum japonicum, 10 parts of peppermint it is mixed
10 mesh mixed-powders are ground into after conjunction, then press 2.2 times of additions distilled water of mixed-powder total weight, warm fire decoction 2.8 hours, often
Ultrasonic vibrating 30min is used after secondary decoction, decocts and ultrasonic vibrating merges extracting solution twice afterwards twice, use vacuum
Pumping filter is filtered extracting solution twice, and medical liquid B is obtained after filtering, stores spare；
Flocculence purification: merging gained medical fluid A and gained medical liquid B, with dilute hydrochloric acid solution adjust the medical fluid pH value after merging to
5.5, fluid temperature is risen to 55 °C, is 75r/min stirring with speed, being added while heating stirring and accounting for medical fluid weight is 0.1%
Chitin modified polymer flocculants and account for the gelatin that medical fluid weight is 0.05%, then carry out purification 2h, vacuum used after purification
The filtering of pumping filter, filtered medical fluid are added rape flower -19 parts of Moringa polypeptide, 5.2 parts of ethylparaben, are mixed evenly
Up to the pharmaceutical composition for treating vaginitis.
Embodiment 2
The preparation of rape flower-Moringa polypeptide:
It is dried after taking the leaf of Moringa of fresh no mildew to be cleaned with clear water, then the leaf of Moringa dried is ground into the Moringa of 30 mesh
Powder；It is dried after taking fresh rape flower wash clean, the rape flower after drying is ground into the rape pollen of 10 mesh；
It is that 2:1 takes moringa powder and rape pollen to be mixed respectively according to mass ratio, pure water is added after mixing and to expect
Liquor ratio is 1:5.0, after adjusting material liquid pH value is 9.0 and then feed liquid is heated to 50 DEG C again, alkali protease is added and is sufficiently stirred
Uniformly, enzymatic hydrolysis 30min is carried out, 100 DEG C of progress destroy the enzyme treatments is heated to, first time enzymolysis liquid pH value is then adjusted to 6.5 again
Neutral proteinase is added according to the 2% of substrate solution weight afterwards, trypsase is added, the bottom of according to according to the 2% of substrate solution weight
Papain is added in the 3% of object solution weight, then is passed through high-pressure pulse electric, opens under conditions of high-pressure pulse electric is normally opened
Dynamic microwave is handled, and the pulse number that high-pressure pulse electric is arranged is 8, electric field strength 10Kv.cm-1, microwave is set and is added
Hot temperature is 55 DEG C, microwave power be 700W processing the time be 30min, close electromagnetic field after enzymatic hydrolysis, be again started up microwave
Temperature is risen to 95 DEG C, maintains 8min to carry out destroy the enzyme treatment, is cooled to room temperature to obtain Peptides liquid；
8.5min is centrifuged with the revolving speed of 5500r/min in Peptides liquid, then takes supernatant in 60 DEG C of progress vacuum
Concentration carries out being freeze-dried up to rape flower-Moringa polypeptide at -55 DEG C；
The preparation of chitin modified polymer flocculants: the chitosan by deacetylation higher than 90% is washed, is air-dried, after constant weight,
The chitosan is dissolved in the acetum that concentration is 0.1mol/L, magnetic agitation is made into the chitosan acetic acid that mass concentration is 2%
Solution；It takes the 150mL solution to be fitted into the three-necked flask with thermometer and stirring rod, citric acid solid is added, lead to nitrogen gas stirring
It is started to warm up after making it dissolve to 65 °C, ammonium ceric nitrate 0.2g is added three times in 30min, continues magnetic force in nitrogen atmosphere and stirs
Reaction 3h is mixed, stops logical nitrogen, is cooled to room temperature to reaction system and obtains thick graft product, settled using acetone, ethanolic extraction
2h, vacuum are dried overnight, up to chitin modified polymer flocculants after grinding；
Separating out alcohol method extract: take 22 parts of frutus cnidii, 25 parts of Cortex Phellodendri, 23 parts of the fruit of summer cypress, 21 parts of rhizoma cyperi, 20 parts of kuh-seng, 25 parts of sargentodoxa cuneata,
The mixed-powder for being ground into 20 mesh is carried out after 10 parts of curcuma zedoary, 18 parts of field pennycress mixing, is added relative to 4.2 times of mixed-powder quality
83%v/v ethyl alcohol, heating and refluxing extraction 1 hour, refluxing extraction twice, every time reflux after use ultrasonic vibrating
30min merges the solution that refluxing extraction obtains respectively twice to obtain mixing phegma；The remaining filter residue powder of refluxing extraction
It is extracted using percolation, makees solvent with the ethyl alcohol of 50%v/v, impregnate 24 hours, with the speed of 2.2ml per minute slowly diacolation,
Collect the percolate of 5 times of amounts；It is filtered after mixing phegma is merged with percolate using vacuum pump nutsch filter, medicine is obtained after filtering
Liquid A, storage is spare, and filter paper specification used in vacuum pump nutsch filter is middling speed quantitative filter paper, and aperture is 30 microns；
Decocting method extracts: 19.5 parts of extracting honeysuckle, 14 parts of dried orange peel, 15 parts of the leaf of bamboo, 10 parts of Chinese lantern, 10.5 parts of hypericum japonicum, peppermint
10 mesh mixed-powders are ground into after 9.5 parts of mixing, then press 2 times of addition distilled water of mixed-powder total weight, it is small that warm fire decocts 2.2
When, ultrasonic vibrating 30min is used after decocting every time, decocts and ultrasonic vibrating merges extracting solution twice afterwards twice, make
Extracting solution twice is filtered with vacuum pump nutsch filter, medical liquid B is obtained after filtering, is stored spare；
Flocculence purification: merging gained medical fluid A and gained medical liquid B, the medical fluid pH value after being merged with dilute hydrochloric acid solution adjusting to 7,
Fluid temperature is risen to 50 °C, is 65r/min stirring with speed, is added while heating stirring and accounts for the shell that medical fluid weight is 0.1%
The polymer-modified flocculant of glycan and the gelatin that medical fluid weight is 0.05% is accounted for, then carries out purification 2h, vacuum pumping is used after purification
Rape flower-totally 5.5 parts of 15 parts of Moringa polypeptide, ethylparaben and propylben is added in filter filtering, filtered medical fluid, mixes
Close the pharmaceutical composition stirred evenly up to for treating vaginitis.
Embodiment 3
The preparation of rape flower-Moringa polypeptide:
It is dried after taking the leaf of Moringa of fresh no mildew to be cleaned with clear water, then the leaf of Moringa dried is ground into the Moringa of 20 mesh
Powder；It is dried after taking fresh rape flower wash clean, the rape flower after drying is ground into the rape pollen of 20 mesh；
It is that 2:1 takes moringa powder and rape pollen to be mixed respectively according to mass ratio, pure water is added after mixing and to expect
Liquor ratio is 1:7.0, after adjusting material liquid pH value is 9.0 and then feed liquid is heated to 53 DEG C again, alkali protease is added and is sufficiently stirred
Uniformly, enzymatic hydrolysis 22min is carried out, 100 DEG C of progress destroy the enzyme treatments is heated to, first time enzymolysis liquid pH value is then adjusted to 6.0 again
Neutral proteinase is added according to the 2% of substrate solution weight afterwards, trypsase is added, the bottom of according to according to the 2% of substrate solution weight
Papain is added in the 3% of object solution weight, then is passed through high-pressure pulse electric, opens under conditions of high-pressure pulse electric is normally opened
Dynamic microwave is handled, and the pulse number that high-pressure pulse electric is arranged is 7, electric field strength 13Kv.cm-1, microwave is set and is added
Hot temperature is 54 DEG C, microwave power be 500W processing the time be 23min, close electromagnetic field after enzymatic hydrolysis, be again started up microwave
Temperature is risen to 100 DEG C, maintains 9.5min to carry out destroy the enzyme treatment, is cooled to room temperature to obtain Peptides liquid；
8min is centrifuged with the revolving speed of 6000r/min in Peptides liquid, then takes supernatant dense in 53 DEG C of progress vacuum
Contracting carries out being freeze-dried up to rape flower-Moringa polypeptide at -50 DEG C；
The preparation of chitin modified polymer flocculants: the chitosan by deacetylation higher than 90% is washed, is air-dried, after constant weight,
The chitosan is dissolved in the acetum that concentration is 0.1mol/L, magnetic agitation is made into the chitosan acetic acid that mass concentration is 2%
Solution；It takes the 150mL solution to be fitted into the three-necked flask with thermometer and stirring rod, citric acid solid is added, lead to nitrogen gas stirring
It is started to warm up after making it dissolve to 68 °C, ammonium ceric nitrate 0.2g is added three times in 30min, continues magnetic force in nitrogen atmosphere and stirs
Reaction 3h is mixed, stops logical nitrogen, is cooled to room temperature to reaction system and obtains thick graft product, settled using acetone, ethanolic extraction
2.5h, vacuum are dried overnight, up to chitin modified polymer flocculants after grinding；
Separating out alcohol method extract: take 20 parts of frutus cnidii, 21 parts of Cortex Phellodendri, 25 parts of the fruit of summer cypress, 24 parts of rhizoma cyperi, 23 parts of kuh-seng, 24 parts of sargentodoxa cuneata,
The mixed-powder for being ground into 20 mesh is carried out after 15 parts of curcuma zedoary, 10 parts of field pennycress mixing, is added relative to 4 times of mixed-powder quality
The ethyl alcohol of 82%v/v, heating and refluxing extraction 1.5 hours, refluxing extraction twice, used ultrasonic vibrating after reflux every time
30min merges the solution that refluxing extraction obtains respectively twice to obtain mixing phegma；The remaining filter residue powder of refluxing extraction
It is extracted using percolation, makees solvent with the ethyl alcohol of 50%v/v, impregnate 24 hours, with the speed of 2.8ml per minute slowly diacolation,
Collect the percolate of 5 times of amounts；It is filtered after mixing phegma is merged with percolate using vacuum pump nutsch filter, medicine is obtained after filtering
Liquid A, storage is spare, and filter paper specification used in vacuum pump nutsch filter is middling speed quantitative filter paper, and aperture is 50 microns；
Decocting method extracts: 18.5 parts of extracting honeysuckle, 12 parts of dried orange peel, 13.5 parts of the leaf of bamboo, 12 parts of Chinese lantern, 11 parts of hypericum japonicum, peppermint 8
10 mesh mixed-powders are ground into after part mixing, then press 2.8 times of addition distilled water of mixed-powder total weight, warm fire decocts 2 hours,
Ultrasonic vibrating 30min is used after decocting every time, decocts and ultrasonic vibrating merges extracting solution twice afterwards twice, using true
Empty pumping filter is filtered extracting solution twice, and medical liquid B is obtained after filtering, stores spare；
Flocculence purification: merging gained medical fluid A and gained medical liquid B, with dilute hydrochloric acid solution adjust the medical fluid pH value after merging to
6.5, fluid temperature is risen to 65 °C, is 80r/min stirring with speed, being added while heating stirring and accounting for medical fluid weight is 0.1%
Chitin modified polymer flocculants and account for the gelatin that medical fluid weight is 0.05%, then carry out purification 2h, vacuum used after purification
The filtering of pumping filter, filtered medical fluid are added rape flower -16 parts of Moringa polypeptide, 5.8 parts of propylben, are mixed evenly
Up to the pharmaceutical composition for treating vaginitis.
Embodiment 4
The preparation of rape flower-Moringa polypeptide:
It is dried after taking the leaf of Moringa of fresh no mildew to be cleaned with clear water, then the leaf of Moringa dried is ground into the Moringa of 30 mesh
Powder；It is dried after taking fresh rape flower wash clean, the rape flower after drying is ground into the rape pollen of 20 mesh；
It is that 2:1 takes moringa powder and rape pollen to be mixed respectively according to mass ratio, pure water is added after mixing and to expect
Liquor ratio is 1:6.0, after adjusting material liquid pH value is 9.0 and then feed liquid is heated to 60 DEG C again, alkali protease is added and is sufficiently stirred
Uniformly, enzymatic hydrolysis 20min is carried out, 100 DEG C of progress destroy the enzyme treatments is heated to, first time enzymolysis liquid pH value is then adjusted to 7.0 again
Neutral proteinase is added according to the 2% of substrate solution weight afterwards, trypsase is added, the bottom of according to according to the 2% of substrate solution weight
Papain is added in the 3% of object solution weight, then is passed through high-pressure pulse electric, opens under conditions of high-pressure pulse electric is normally opened
Dynamic microwave is handled, and the pulse number that high-pressure pulse electric is arranged is 6, electric field strength 15Kv.cm-1, microwave is set and is added
Hot temperature is 50 DEG C, microwave power be 600W processing the time be 20min, close electromagnetic field after enzymatic hydrolysis, be again started up microwave
Temperature is risen to 96 DEG C, maintains 8.5min to carry out destroy the enzyme treatment, is cooled to room temperature to obtain Peptides liquid；
10min is centrifuged with the revolving speed of 4500r/min in Peptides liquid, then takes supernatant dense in 50 DEG C of progress vacuum
Contracting carries out being freeze-dried up to rape flower-Moringa polypeptide at -58 DEG C；
The preparation of chitin modified polymer flocculants: the chitosan by deacetylation higher than 90% is washed, is air-dried, after constant weight,
The chitosan is dissolved in the acetum that concentration is 0.1mol/L, magnetic agitation is made into the chitosan acetic acid that mass concentration is 2%
Solution；It takes the 150mL solution to be fitted into the three-necked flask with thermometer and stirring rod, citric acid solid is added, lead to nitrogen gas stirring
It is started to warm up after making it dissolve to 70 °C, ammonium ceric nitrate 0.2g is added three times in 30min, continues magnetic force in nitrogen atmosphere and stirs
Reaction 3h is mixed, stops logical nitrogen, is cooled to room temperature to reaction system and obtains thick graft product, settled using acetone, ethanolic extraction
2.8h, vacuum are dried overnight, up to chitin modified polymer flocculants after grinding；
Separating out alcohol method extract: take 25 parts of frutus cnidii, 20 parts of Cortex Phellodendri, 22 parts of the fruit of summer cypress, 23 parts of rhizoma cyperi, 21 parts of kuh-seng, 20 parts of sargentodoxa cuneata,
The mixed-powder for being ground into 20 mesh is carried out after 18 parts of curcuma zedoary, 16 parts of field pennycress mixing, is added relative to 4.8 times of mixed-powder quality
80%v/v ethyl alcohol, heating and refluxing extraction 1.2 hours, refluxing extraction twice, every time reflux after use ultrasonic vibrating
30min merges the solution that refluxing extraction obtains respectively twice to obtain mixing phegma；The remaining filter residue powder of refluxing extraction
It is extracted using percolation, makees solvent with the ethyl alcohol of 50%v/v, impregnate 24 hours, with the speed of 3ml per minute slowly diacolation, received
Collect the percolate of 5 times of amounts；It is filtered after mixing phegma is merged with percolate using vacuum pump nutsch filter, medical fluid is obtained after filtering
A, storage is spare, and filter paper specification used in vacuum pump nutsch filter is middling speed quantitative filter paper, and aperture is 40 microns；
Decocting method extracts: 20 parts of extracting honeysuckle, 13.5 parts of dried orange peel, 14 parts of the leaf of bamboo, 8 parts of Chinese lantern, 10 parts of hypericum japonicum, 9 parts of peppermint
10 mesh mixed-powders are ground into after mixing, then press 2.5 times of additions distilled water of mixed-powder total weight, warm fire decoction 3 hours, often
Ultrasonic vibrating 30min is used after secondary decoction, decocts and ultrasonic vibrating merges extracting solution twice afterwards twice, use vacuum
Pumping filter is filtered extracting solution twice, and medical liquid B is obtained after filtering, stores spare；
Flocculence purification: merging gained medical fluid A and gained medical liquid B, the medical fluid pH value after being merged with dilute hydrochloric acid solution adjusting to 5,
Fluid temperature is risen to 60 °C, is 70r/min stirring with speed, is added while heating stirring and accounts for the shell that medical fluid weight is 0.1%
The polymer-modified flocculant of glycan and the gelatin that medical fluid weight is 0.05% is accounted for, then carries out purification 2h, vacuum pumping is used after purification
Filter filtering, filtered medical fluid are added rape flower -17 parts of Moringa polypeptide, 5 parts of propylben, are mixed evenly i.e. handy
In the pharmaceutical composition for the treatment of vaginitis.
Embodiment 5
The preparation of rape flower-Moringa polypeptide:
It is dried after taking the leaf of Moringa of fresh no mildew to be cleaned with clear water, then the leaf of Moringa dried is ground into the Moringa of 20 mesh
Powder；It is dried after taking fresh rape flower wash clean, the rape flower after drying is ground into the rape pollen of 10 mesh；
It is that 2:1 takes moringa powder and rape pollen to be mixed respectively according to mass ratio, pure water is added after mixing and to expect
Liquor ratio is 1:8.0, after adjusting material liquid pH value is 9.0 and then feed liquid is heated to 56 DEG C again, alkali protease is added and is sufficiently stirred
Uniformly, enzymatic hydrolysis 28min is carried out, 100 DEG C of progress destroy the enzyme treatments is heated to, first time enzymolysis liquid pH value is then adjusted to 7.5 again
Neutral proteinase is added according to the 2% of substrate solution weight afterwards, trypsase is added, the bottom of according to according to the 2% of substrate solution weight
Papain is added in the 3% of object solution weight, then is passed through high-pressure pulse electric, opens under conditions of high-pressure pulse electric is normally opened
Dynamic microwave is handled, and the pulse number that high-pressure pulse electric is arranged is 8, electric field strength 20Kv.cm-1, microwave is set and is added
Hot temperature is 53 DEG C, microwave power be 400W processing the time be 28min, close electromagnetic field after enzymatic hydrolysis, be again started up microwave
Temperature is risen to 97 DEG C, maintains 9min to carry out destroy the enzyme treatment, is cooled to room temperature to obtain Peptides liquid；
9min is centrifuged with the revolving speed of 5000r/min in Peptides liquid, then takes supernatant dense in 58 DEG C of progress vacuum
Contracting carries out being freeze-dried up to rape flower-Moringa polypeptide at -60 DEG C；
The preparation of chitin modified polymer flocculants: the chitosan by deacetylation higher than 90% is washed, is air-dried, after constant weight,
The chitosan is dissolved in the acetum that concentration is 0.1mol/L, magnetic agitation is made into the chitosan acetic acid that mass concentration is 2%
Solution；It takes the 150mL solution to be fitted into the three-necked flask with thermometer and stirring rod, citric acid solid is added, lead to nitrogen gas stirring
It is started to warm up after making it dissolve to 75 °C, ammonium ceric nitrate 0.2g is added three times in 30min, continues magnetic force in nitrogen atmosphere and stirs
Reaction 3h is mixed, stops logical nitrogen, is cooled to room temperature to reaction system and obtains thick graft product, settled using acetone, ethanolic extraction
2.2h, vacuum are dried overnight, up to chitin modified polymer flocculants after grinding；
Separating out alcohol method extract: take 23 parts of frutus cnidii, 24 parts of Cortex Phellodendri, 21 parts of the fruit of summer cypress, 20 parts of rhizoma cyperi, 22 parts of kuh-seng, 23 parts of sargentodoxa cuneata,
The mixed-powder for being ground into 20 mesh is carried out after 20 parts of curcuma zedoary, 20 parts of field pennycress mixing, is added relative to 5 times of mixed-powder quality
The ethyl alcohol of 84%v/v, heating and refluxing extraction 1.8 hours, refluxing extraction twice, used ultrasonic vibrating after reflux every time
30min merges the solution that refluxing extraction obtains respectively twice to obtain mixing phegma；The remaining filter residue powder of refluxing extraction
It is extracted using percolation, makees solvent with the ethyl alcohol of 50%v/v, impregnate 24 hours, with the speed of 2ml per minute slowly diacolation, received
Collect the percolate of 5 times of amounts；It is filtered after mixing phegma is merged with percolate using vacuum pump nutsch filter, medical fluid is obtained after filtering
A, storage is spare, and filter paper specification used in vacuum pump nutsch filter is middling speed quantitative filter paper, and aperture is 45 microns；
Decocting method extracts: 19 parts of extracting honeysuckle, 15 parts of dried orange peel, 13 parts of the leaf of bamboo, 11 parts of Chinese lantern, 11.5 parts of hypericum japonicum, peppermint 8.5
10 mesh mixed-powders are ground into after part mixing, then press 3 times of addition distilled water of mixed-powder total weight, warm fire decocts 2.5 hours,
Ultrasonic vibrating 30min is used after decocting every time, decocts and ultrasonic vibrating merges extracting solution twice afterwards twice, using true
Empty pumping filter is filtered extracting solution twice, and medical liquid B is obtained after filtering, stores spare；
Flocculence purification: merging gained medical fluid A and gained medical liquid B, the medical fluid pH value after being merged with dilute hydrochloric acid solution adjusting to 6,
Fluid temperature is risen to 70 °C, is 60r/min stirring with speed, is added while heating stirring and accounts for the shell that medical fluid weight is 0.1%
The polymer-modified flocculant of glycan and the gelatin that medical fluid weight is 0.05% is accounted for, then carries out purification 2h, vacuum pumping is used after purification
Rape flower-totally 6 parts of 20 parts of Moringa polypeptide, ethylparaben and propylben, mixing is added in filter filtering, filtered medical fluid
It stirs evenly up to the pharmaceutical composition for treating vaginitis.
In order to verify the curative effect for the pharmaceutical composition for treating vaginitis that the present invention is prepared, by the embodiment of the present invention
1 ~ 5 pharmaceutical composition being prepared is prepared into lotion for clinical testing treatment.It is with commercially available similar drug lotion simultaneously
Control group, clinical testing treatment situation are as follows.
1 data and method
1.1 general information
The gynecology colpitis Patients with 300 Cases that in January, 2017 in January, 2018 is chosen in the receiving treatment of identical hospital is analysis pair
As being randomly divided into 6 groups every group 50 after carrying out serial number to 300 patients, 1 ~ 5 group is respectively adopted the embodiment of the present invention 1 ~ 5
The pharmaceutical composition lotion that method is prepared is treated, and the 6th group is treated using the drug lotion of control group.1st ~ 5 group
Age is 18 ~ 50 years old, average (32.71 ± 2.81) year, 18 ~ 47 years old control group age, average (33.56 ± 2.32) year, two groups
The age, the course of disease, symptom, coincident with severity degree of condition and in terms of through statistical analysis there was no significant difference (p
>0.05)。
1.2 clinical symptoms, selection criteria
There are following symptoms when patient's admission: pruritus vulvue, urination process consciously obvious burning heat sensation, leukorrhea diacrisis,
There is swelling sense, degree is different.
It is included in standard: being confirmed as vaginitis (monilial vaginitis, trichomonas vaginitis, bacterial vaginitis) on inspection
；Sexual life is normal；It has gained some understanding to received therapeutic scheme difference and voluntarily cooperates.
Exclusion criteria: there are serious hepatic renal dysfunctions；Complication with diabetes；The prolonged application antimicrobial before this is treated
Object, immune suppressant drug；In nursing period or the gestational period；It is reluctant partner treatment.
1.3 treatment method
It takes the pharmaceutical composition lotion 10ml warm water of Example 1 ~ 5 to mix to 100ml respectively, clean vulva and rinses yin
Road, 1 time a day.It takes control group drug lotion 10ml warm water to mix to 100ml, cleans vulva and washing vagina, daily 1
It is secondary.Every group, with 7 days as a course for the treatment of, is continuously treated 3 courses for the treatment of.Prohibit sexual life during treatment and with other drugs, pays attention to vulva
Health, underpants, bath towel are sterilized with boiling water, the pungent stimulation food of fasting.The forbidden vaginadouche of intermenstrual period, after menstruation 3d after
Continuous treatment.
1.4 efficacy assessment standard
After treatment end, 3~5d check after each clean period in continuous 3 months is evaluated patient clinical curative effect, is divided into
It cures, effective and invalid 3 grades.Cure: clinical symptoms completely disappear after drug withdrawal, no pruritus vulvue, pain, frequent micturition etc., stick
Film checks that normally 3 menstrual cycles of being discontinued are without recurrence without redness, blocky erythema fading, vaginal fluid；It is effective: clinical symptoms
It is obviously improved, the symptoms such as pruritus vulvue, pain, frequent micturition are obviously controlled, and mucous membrane redness and blocky erythema are substantially reduced, vagina
Secretion check that normal and other symptoms exist or other symptoms disappear, mitigate and vaginal fluid check it is abnormal；It is invalid: on
It states to be not improved before clinical symptoms and sign are relatively treated and even aggravate, or recurrent exerbation, vaginal secretion in the short time after improving
Quality testing, which is looked into, not to improve.Total effective rate=(cure+effective+effectively)/total number of cases × 100%.
1.5 result
Curative effect after 1 patient of table treatment compares
1.6 recurrence
Random access is carried out to patient after the course for the treatment of, counts the recurrence rate occurred during the visit.Statistical result is as shown in table 2 below.
Gynecology colpitis recurrence compares after 2 different pharmaceutical preparation for treating of table
Specific case 1:
Mrs Huang, 25 years old, patient leukorrhea itching of vulva was remaining more than a year, and vaginal fluid homogeneous lean, secretion increase on inspection, have
Fishlike smell, while with burning heat sensation.Using 1 pharmaceutical composition lotion of the embodiment of the present invention, one time a day, after continuous use 12 days, fully recover from an illness
More, follow-up later six months does not recur.
Specific case 2:
Granddaughter scholar, 32 years old, patient's pruritus vulvue discovery vaginal mucosa when checking was congested, was dispersed in bleeding spot, leukorrhea is in yellowish-white
Color wash or yellow green purulent secretion, normal show bubble, patient have abdominal pain when telling sexual intercourse.It gets married 2 years unpregnancies always.
Implement the pharmaceutical composition lotion in 3 using the present invention, daily 1 cleans 1 time, after a course for the treatment of is used continuously, by gynecologial examination
Leukorrhea significantly reduces, and without pruritis, the course for the treatment of is pregnant vulva after terminating 6 months.
The above embodiment of the present invention scheme is only the description of the invention and cannot limit the present invention, is pointed out in claim
Product of the present invention constituent, component ratio, the range of preparation method parameter, and above-mentioned explanation does not point out ginseng of the present invention
Therefore several ranges any change within the meaning and scope equivalent to the claims of the present invention, is all considered to be
It is included within the scope of the claims.
The present invention is and to pass through creative work by multidigit vaginitis treatment and nursing staff's long-term work experience accumulation
It creates and goes out, the present invention can effectively extract Chinese herb medicine, the active constituent being able to maintain in Chinese herbal medicine, the medicine group
Conjunction is significant to vaginitis disease therapeutic effect, and the recurrence rate of vaginitis is effectively reduced, mitigates the pain of patient.
Claims (4)
1. a kind of for treating the pharmaceutical composition of vaginitis, characterized in that the pharmaceutical composition raw material each group synthetic ingredient and its again
Measure portion rate are as follows: rape flower -15 ~ 20 parts of Moringa polypeptide, 20 ~ 25 parts of frutus cnidii, 20 ~ 25 parts of Cortex Phellodendri, 20 ~ 25 parts of the fruit of summer cypress, perfume
Attached 20 ~ 25 parts, 20 ~ 25 parts of kuh-seng, 20 ~ 25 parts of sargentodoxa cuneata, 10 ~ 20 parts of curcuma zedoary, 10 ~ 20 parts of field pennycress, 18 ~ 20 parts of honeysuckle,
12 ~ 15 parts of dried orange peel, 12 ~ 15 parts of the leaf of bamboo, 8 ~ 12 parts of Chinese lantern, 10 ~ 12 parts of hypericum japonicum, 8 ~ 10 parts of peppermint；
The rape flower-Moringa polypeptide the preparation method comprises the following steps:
A. it is dried after taking the leaf of Moringa of fresh no mildew to be cleaned with clear water, the leaf of Moringa dried is then ground into 20 ~ 30 purposes
Moringa powder；It is dried after taking fresh rape flower wash clean, the rape flower after drying is ground into the rape of 10 ~ 20 mesh
Pollen；
B. it is that 2:1 takes moringa powder and rape pollen to be mixed respectively according to mass ratio, pure water is added after mixing and makes
Solid-liquid ratio is 1:5.0 ~ 1:10.0, and after adjusting material liquid pH value is 9.0 and then feed liquid is heated to 50 ~ 60 DEG C again, alkaline egg is added
White enzyme stirs, and carries out 20 ~ 30min of enzymatic hydrolysis, is heated to 100 DEG C of progress destroy the enzyme treatments, then again digests first time
Liquid pH value be adjusted to 6.0 ~ 7.5 after according to substrate solution weight 2% be added neutral proteinase, according to the 2% of substrate solution weight
Trypsase is added, papain is added according to the 3% of substrate solution weight, then is passed through high-pressure pulse electric, in high-voltage pulse
Start microwave under conditions of electric field is normally opened to be handled, the pulse number that high-pressure pulse electric is arranged is 6 ~ 8, and electric field strength is
10~20Kv.cm-1, setting microwave heating temperature be 50 ~ 55 DEG C, microwave power be 400 ~ 800W handle the time be 20 ~ 30min, enzyme
Electromagnetic field is closed after solution, microwave is again started up by temperature and rises to 95 ~ 100 DEG C, and 8 ~ 10min is maintained to carry out destroy the enzyme treatment, it is cold
But Peptides liquid is obtained to room temperature；
C. 8 ~ 10min is centrifuged with the revolving speed of 4000 ~ 6000r/min in Peptides liquid, then takes supernatant 50 ~ 60
It DEG C is concentrated in vacuo, carries out being freeze-dried up to rape flower-Moringa polypeptide at -60 ~ -50 DEG C.
2. a kind of prepare the method described in claim 1 for treating the pharmaceutical composition of vaginitis, characterized in that including following
Step:
The preparation of the chitin modified polymer flocculants of step 1): the chitosan that deacetylation is higher than 90% is washed, is air-dried,
After constant weight, which is dissolved in the acetum that concentration is 0.1mol/L, magnetic agitation is made into the shell that mass concentration is 2%
Glycan acetum；It takes the 150mL solution to be fitted into the three-necked flask with thermometer and stirring rod, citric acid solid is added, lead to
Nitrogen gas stirring is started to warm up after making it dissolve to 65 ~ 75 °C, is added three times ammonium ceric nitrate 0.2g in 30min, in nitrogen atmosphere
Continue magnetic agitation and react 3h, stops logical nitrogen, be cooled to room temperature to reaction system and obtain thick graft product, it is heavy using acetone
Drop, 2 ~ 3h of ethanolic extraction, vacuum are dried overnight, up to chitin modified polymer flocculants after grinding；
Step 2) separating out alcohol method extracts: taking 20 ~ 25 parts of frutus cnidii, 20 ~ 25 parts of Cortex Phellodendri, 20 ~ 25 parts of the fruit of summer cypress, 20 ~ 25 parts of rhizoma cyperi, hardship
The mixed powder for being ground into 20 mesh is carried out after 20 ~ 25 parts of ginseng, 20 ~ 25 parts of sargentodoxa cuneata, 10 ~ 20 parts of curcuma zedoary, 10 ~ 20 parts of field pennycress mixing
End, ethyl alcohol of the addition relative to 4 ~ 5 times of mixed-powder quality of 80% ~ 85%v/v, heating and refluxing extraction 1 ~ 2 hour, refluxing extraction
Twice, ultrasonic vibrating 30min is used after reflux every time, the solution that refluxing extraction obtains respectively twice is merged
To mixing phegma；The remaining filter residue powder of refluxing extraction is extracted using percolation, does solvent, dipping 24 with the ethyl alcohol of 50%v/v
Hour, with the speed of 2 ~ 3ml per minute slowly diacolation, collect the percolates of 5 times of amounts；After mixing phegma is merged with percolate
It is filtered using vacuum pump nutsch filter, medical fluid A is obtained after filtering, storage is spare, and filter paper specification used in vacuum pump nutsch filter is
Middling speed quantitative filter paper, aperture are 30 ~ 50 microns；
Step 3) decocting method extracts: 18 ~ 20 parts of honeysuckle, 12 ~ 15 parts of dried orange peel, 12 ~ 15 parts of the leaf of bamboo, 8 ~ 12 parts of Chinese lantern, nostoc commune
10 mesh mixed-powders are ground into after 10 ~ 12 parts of grass, 8 ~ 10 parts of peppermint mixing, are then steamed by 2 ~ 3 times of additions of mixed-powder total weight
Distilled water, warm fire decoct 2 ~ 3 hours, every time decoct after use ultrasonic vibrating 30min, decoct and ultrasonic vibrating twice after
Merge extracting solution twice, extracting solution twice is filtered using vacuum pump nutsch filter, medical liquid B is obtained after filtering, stores spare；
The purification of step 4) flocculence: merging medical liquid B obtained by medical fluid A obtained by step 1) and step 2), is adjusted and is closed with dilute hydrochloric acid solution
Fluid temperature is risen to 50 ~ 70 DEG C to 5 ~ 7 by the medical fluid pH value after and, with speed be 60 ~ 80r/min stirring, heating stirring it is same
When be added and account for chitin modified polymer flocculants that medical fluid weight is 0.1% and account for the gelatin that medical fluid weight is 0.05%, then into
Row purification 2h, is filtered after purification using vacuum pump nutsch filter, rape flower -15 ~ 20 parts of Moringa polypeptide, Buddhist nun is added in filtered medical fluid
Golden 5 ~ 6 parts of ester is moored, is mixed evenly up to the pharmaceutical composition for treating vaginitis.
3. a kind of method for treating the pharmaceutical composition of vaginitis according to claim 1, characterized in that the medicine
Object combination can be made into lotion, spray, cream, suppository, tablet according to conventional dose technique.
4. a kind of method for preparing the pharmaceutical composition for treating vaginitis according to claim 2, characterized in that described
Paraben esters be one or both of ethylparaben, propylben combination.
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Application publication date: 20190104 |
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| WW01 | Invention patent application withdrawn after publication |