CN103202920B - Chinese medicine composition with antibacterial action - Google Patents
Chinese medicine composition with antibacterial action Download PDFInfo
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- CN103202920B CN103202920B CN201310050512.4A CN201310050512A CN103202920B CN 103202920 B CN103202920 B CN 103202920B CN 201310050512 A CN201310050512 A CN 201310050512A CN 103202920 B CN103202920 B CN 103202920B
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Abstract
The present invention relates to a kind of Chinese medicine composition with antibacterial action.Specifically, the present invention relates to a kind of Chinese medicine composition, including shrubby sophora extract and green-tea extract, and optional pharmaceutically acceptable auxiliary material.Chinese medicine composition of the present invention has good antibacterial effect.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, is related to a kind of composition with antibacterial action, is wrapped more particularly to one kind
Include the Chinese medicine composition of shrubby sophora extract and green-tea extract.
Background technology
While microorganism brings tremendous contribution to human progress, also moment prestige assists human health.For example, it is known that intestines
Escherichia (E. coli) is commonly referred to as Escherichia coli, is that Escherich had found in 1885, in considerably long one section
It is interior, the part of normal the gut flora is taken as always, it is believed that is non-pathogenic bacteria.Until 20 middle of century, just recognize
The Escherichia coli of some special serotypes have pathogenicity to humans and animals, especially to baby and cub（Fowl）, often cause serious abdomen
Rush down and septicemia, it is a kind of common prokaryotes, and enteropathogenic E. Coli is divided into 5 according to different biological characteristicses
Class：Enteropathogenic E. Coli (EPEC), enterotoxigenic E.Coli (ETEC), enteroinvasive E.Coli (EIEC), enterorrhagia
Property Escherichia coli (EHEC), intestines adhesion Escherichia coli (EAEC).
Escherichia coli are that a kind of foremost bacterium, main parasitic account for enterobacteriaceae in big enteral in humans and animals enteron aisle
In 1%.It is a kind of both ends blunt circle, can moves, nonspore-bearing Gram-negative brevibacterium.Escherichia coli can synthesize vitamin B and
K, it is not pathogenic under the conditions of normal perch；If inflammation can be caused into gall-bladder, bladder etc..Detected in water and food, it is believed that
It is the index of faecal contamination.Hygienic Standard of the coliform group count frequently as drinking-water, food or medicine.
Escherichia coli O 157：H7 serotype category enterohemorrhagic escherichia colis, from nineteen eighty-two since the U.S. is found first, bag
Include many countries such as China all to have been reported that, and day by day increase.Japan in recent years caused by the food pollution bacterium several break out greatly,
It is especially noticeable.In the pathogenic entero becteria that America ＆ Canada is generally separated, it has come second or the 3rd at present.Greatly
Enterobacteria O 157：H7 causes enterohemorrhagic to be suffered from diarrhoea, and about 2%~7% patient can develop into hemolytic uremic syndrome, Er Tongyu
Old man is easiest to latter event occur.Enteropathogenic E. Coli causes outbreak of disease by polluting drinking-water, food, amusement water body
Prevalence, serious patient can threat to life.
Escherichia coli are Gram-negative brevibacterium, 0.5 × 1~3 microns of size.Whole body flagellum, it can move, no gemma.
The various saccharides that can ferment production acid, aerogenesis, it is the normal perch bacterium in humans and animals enteron aisle, enters intestines with lactation after baby due
Road, accompany all the life with people, its metabolic activity can suppress the microorganism growth of decomposing protein in enteron aisle, reduce breaks down proteins production
Harm of the thing to human body, moreover it is possible to synthesize vitamin B and K, and have the colicine of bactericidal action.Under the conditions of normal perch not
Cause a disease.If but inflammation can be caused into gall-bladder, bladder etc..The amount reproduction in enteron aisle, it is several to account for the 1/3 of excrement dry weight.In ring
In the case that border health is bad, often it is dispersed in excrement in surrounding environment.If this bacterium is detected in water and food, it is believed that be by
The index of fecal pollution, so as to there is the presence of enteric pathogenic bacteria.Therefore, coliform group count（Or colititer）Frequently as drink
Water and food（Or medicine）Hygienic Standard.National regulation, coliform count is not to be exceeded 3 in every liter of drinking water）Large intestine
The antigenic component of bacillus is complicated, can be divided into somatic antigen（O）, flagellar antigen（H）And surface antigen（K）, the latter has anti-body to gulp down
Bite the ability with anticomplement.According to the difference of somatic antigen, Escherichia coli can be divided into it is 150 many types of, wherein there is 16 serotypes
For enteropathogenic E. Coli, often cause epidemic infantile diarrhea and adult's pleurisy.
The antibacterial action of some chemicalses is obvious to all, but substantial amounts of antibiotic application, is either applied to
Clinic is still used for other industries such as livestock culture, aquaculture, may trigger a series of social concern such as resistance
Sex chromosome mosaicism.Therefore people expect to find effective antibacterial substance from natural products.
The content of the invention
It is an object of the invention to provide a kind of natural Chinese medicine composition with antibacterial activity.The inventors discovered that kuh-seng
Extract can effectively play antibacterial activity after being combined with other plant extracts.
Therefore, one side of the invention provides a kind of Chinese medicine composition with antibacterial activity, including kuh-seng
Extract and green-tea extract, and optional pharmaceutically acceptable auxiliary material.
Chinese medicine composition according to a first aspect of the present invention, wherein the shrubby sophora extract is to be prepared as follows
's：After kuh-seng is crushed with 60% ethanol be extract solution soak, then heating and refluxing extraction 1-3 times；The extract solution of merging is concentrated
To without use watery hydrochloric acid after alcohol taste, until solution ph is 1-2, after elimination insoluble matter, filtrate chloroform extraction, by merging
Aqueous phase adjusts pH value to 9-11 with ammoniacal liquor, then with chloroform extraction, merges organic layer, evaporation of solvent, is dried to obtain
Shrubby sophora extract.
Chinese medicine composition according to a first aspect of the present invention, wherein the shrubby sophora extract is to be prepared as follows
's：Kuh-seng beats powder, sieving, after soaking 2 h as extract solution using 60% ethanol, the h of heating and refluxing extraction 1.5, after extracting 2 times, merges
Extract solution；After extract solution is concentrated into no alcohol taste, add 0.5% hydrochloric acid until solution ph be 1-2, elimination insoluble matter after, filtrate
After chloroform extraction 3 times, aqueous phase adjusts pH value to 9.5-10.5 (such as adjusting pH value to 10), chloroform extraction with ammoniacal liquor
Take 3 times, merge organic layer, evaporation of solvent, be dried to obtain shrubby sophora extract.
Chinese medicine composition according to a first aspect of the present invention, wherein the green-tea extract is to be prepared as follows
's：Green tea is soaked in water, then heating extraction, merges extract solution, concentration, dry, produce.
Chinese medicine composition according to a first aspect of the present invention, wherein the green-tea extract is to be prepared as follows
's：The water that green tea is measured with 5-10 times (such as 8 times) is soaked into 10-15 hours (e.g., from about 12 hours), then in 70 ~ 90 DEG C of (examples
Such as from about 80 DEG C) heating extraction 1-3 times (such as 2 times), each 1-3 hours (e.g., from about 1.5 hours), merge extract solution, filter, it is dense
Contracting, dry, produce.
Chinese medicine composition according to a first aspect of the present invention, wherein the weight of the shrubby sophora extract and green-tea extract ratio
For 1：1-5, preferably 1：2-3, such as 1：2.5.
Chinese medicine composition according to a first aspect of the present invention, wherein the pharmaceutically acceptable auxiliary material is included selected from following
It is one or more：Diluent, adhesive, disintegrant, lubricant etc..These auxiliary materials can use conventional pharmaceutic adjuvant, such as
Diluent can use starch, microcrystalline cellulose etc.；Adhesive can use hydroxypropyl methyl cellulose, polyvinylpyrrolidone
Deng；Disintegrant is with use sodium carboxymethyl starch, PVPP etc.；Lubricant is with use magnesium stearate, talcum powder, superfine silica gel powder
Deng.The ratio of these auxiliary materials in the compositions of the present invention can be not particularly limited, for example, can account for composition total weight 0 ~
99%.Essence of the invention is that finding the combination of Chinese medical extract of the present invention has unexpected antibacterial effect, therefore
Can not particularly it be limited for supplementary product kind therein and its dosage in the compositions of the present invention.
Chinese medicine composition according to a first aspect of the present invention, it is in shapes such as tablet, granule, powder, capsule, pills
Formula.The concrete form of Chinese medicine composition of the present invention should not be particularly limited.
Second aspect of the present invention provides the method for preparing any one of the first aspect present invention Chinese medicine composition, and it is wrapped
Include following steps：
(1) shrubby sophora extract is provided；
(2) green-tea extract is provided；
(3) shrubby sophora extract is mixed with the green-tea extract, wherein being optionally added into pharmaceutically acceptable auxiliary
Material, gained mixture is prepared into appropriate form, produced.
Method according to a second aspect of the present invention, wherein the shrubby sophora extract is prepared as follows：Will
Kuh-seng crush after with 60% ethanol be extract solution immersion, then heating and refluxing extraction 1-3 times；The extract solution of merging is concentrated into nothing
Watery hydrochloric acid is used after alcohol taste, until solution ph is 1-2, after filtering off insoluble matter, filtrate chloroform extraction, by the aqueous phase of merging
PH value is adjusted to 9-11 with ammoniacal liquor, then with chloroform extraction, is merged organic layer, evaporation of solvent, is dried to obtain kuh-seng
Extract.
Method according to a second aspect of the present invention, wherein the shrubby sophora extract is prepared as follows：It is bitter
Ginseng beats powder, sieves, and after soaking 2 h as extract solution using 60% ethanol, the h of heating and refluxing extraction 1.5, after extracting 2 times, merges extraction
Liquid；After extract solution is concentrated into no alcohol taste, add 0.5% hydrochloric acid until solution ph be 1-2 (such as 1.5), elimination insoluble matter
Afterwards, for filtrate with after chloroform extraction 3 times, aqueous phase adjusts pH value to 9.5-10.5 (such as 10), chloroform extraction 3 with ammoniacal liquor
It is secondary, merge organic layer, evaporation of solvent, be dried to obtain shrubby sophora extract.
Method according to a second aspect of the present invention, wherein the green-tea extract is prepared as follows：Will
Green tea is soaked in water, then heating extraction, merges extract solution, concentration, dries, produce.
Method according to a second aspect of the present invention, wherein the green-tea extract is prepared as follows：Will
The water that green tea is measured with 5-10 times (such as 8 times) soaks 10-15 hours (e.g., from about 12 hours), then at 70 ~ 90 DEG C (e.g., from about 80
DEG C) heating extraction 1-3 times (such as 2 times), each 1-3 hours (e.g., from about 1.5 hours), merge extract solution, filtering, concentration, do
It is dry, produce.
Method according to a second aspect of the present invention, wherein the weight of the shrubby sophora extract and green-tea extract ratio is 1：1-
5, preferably 1：2-3, such as 1：2.5.
Appropriate form described in method according to a second aspect of the present invention, wherein step (3) is to be selected from following form：
Tablet, granule, powder, capsule, pill etc..
According to the either side of the present invention, wherein described composition can be used for anti-coli-infection.According to the present invention,
The composition of the present invention can be used for all polymicrobic infections in itself.Therefore, in the third aspect of the invention, there is provided this hair
The purposes of Chinese medicine composition described in bright first aspect, it is used for anti-microbial infection, can be especially useful for anti-coli-infection.
Further, in the fourth aspect of the invention, there is provided Chinese medicine composition described in first aspect present invention is micro- for resisting in preparation
Purposes in the product of biological (such as anti-Escherichia coli)；In one embodiment, there is provided described in first aspect present invention
Chinese medicine composition is being prepared for the purposes in the product of antimicrobial (such as anti-Escherichia coli) infection.
Any technical characteristic possessed by any embodiment of either side or the either side of the present invention is equally applicable
Any embodiment of other any embodiments or other either sides, as long as they will not be conflicting, certainly mutual
Between where applicable, if necessary can individual features be made with appropriate modification.Make to various aspects of the present invention with feature into one below
The description of step.
All documents recited in the present invention, their full content are incorporated herein by reference, and if these are literary
When offering expressed implication and the inconsistent present invention, it is defined by the statement of the present invention.In addition, the various terms that use of the present invention and
Phrase has well known to a person skilled in the art general sense, nonetheless, the present invention remain desirable at this to these terms and
Phrase is described in more detail and explains, the term and phrase referred to is if any inconsistent with common art-recognized meanings, with institute's table of the present invention
The implication stated is defined.
Present inventors have surprisingly discovered that Chinese medicine composition of the present invention has unexpected antibacterial effect.
Embodiment
The present invention is further elaborated with by following instance, further describes various aspects of the invention, but should
The understanding, the scope that following instance is not intended to limit the invention.
First, extract preparation example part
Preparation example 1：Prepare shrubby sophora extract
Kuh-seng is beaten into powder (20-40 mesh), sieved, after soaking 2 h as extract solution using 60% ethanol, heating and refluxing extraction 1.5
H, after extracting 2 times, merge extract solution；After extract solution is concentrated into no alcohol taste, add 0.5% hydrochloric acid until solution ph be 1.5,
After filtering off insoluble matter, filtrate is with after chloroform extraction 3 times, and aqueous phase adjusts pH value to 10 with ammoniacal liquor, chloroform extraction 3 times,
Merge organic layer, evaporation of solvent, dry, obtain shrubby sophora extract.If not otherwise indicated, the extract is used for following reality
Test.
Preparation example 2：Prepare green-tea extract
Green tea is soaked 12 hours with the water of 8 times of amounts, then heating extraction 2 times at 80 DEG C, 1.5 hours every time, merged
Extract solution, filtering, concentration, dry, produce green-tea extract, yield (in terms of the medicinal material that feeds intake) 34.44%.If not otherwise indicated, should
Extract is used for following experiment.
2nd, biological test example part
This test example part comprehensively utilizes agar dilution and broth dilution method bacteriostatic activity detection method measure Chinese medicine carries
Take the antibacterial activity of thing or composition.Reagent be preparation example part obtain extract (single extract either they
Mixture by a certain percentage).In the present invention, when extract combination is using its MIC is determined, for mixing gained to scale
The MIC value that composition measuring obtains；Extract is used alone when determining its MIC, the MIC that determines to obtain for the single extract
Value.
1st, the antibacterial experiment method of single extract sample：Agar dilution
Representative microorganism of the suitable experimental microbial as bacteriostatic activity is selected, determines shrubby sophora extract respectively to golden yellow
Color staphylococcus (Staphylococcus aureus) ATCC 6538 and Escherichia coli (Escherichia coli) 8099
Fungistatic effect, determine minimal inhibitory concentration (MIC).
The preparation of nutrient broth medium：Nutrient broth medium is taken to add the ratio of 1L water to be matched somebody with somebody according to 18g culture mediums
System.Heating water bath is until culture medium is completely dissolved.By the culture medium after being completely dissolved, 9ml is taken as in test tube with pipette.
103.3Pa high pressure steam sterilizations 20-25min.After culture medium constant temp after sterilizing preserves 48-72h, no muddy produce can then make
With.
Fluid nutrient medium is inoculated with：By 2005 editions《Pharmacopeia》Annex XIII C bacteriums, mould and saccharomycete count bacterium solution system
It is standby：Bacterium to be measured is inoculated with into nutrient broth medium, cultivates 18 ~ 24 h.
Make bacterial suspension：By bacterium solution (about 108 cfu/ml) of the concentration equivalent to 0.5 Maxwell opacity tube, with 0.9% nothing
Bacterium sodium chloride solution is configured to concentration equivalent to 107The suspension of cfu/ml bacterium solution.
The preparation of the making nutrient agar of drug containing flat board：The desired amount of nutrient agar is weighed, according to every
The ratio that 32 g nutrient agars add 1 L water adds distilled water, and agitating and heating is boiled to being completely dissolved, and is sub-packed in test tube
In, every mL of test tube 19.121 DEG C of min of autoclaving 30, it is placed in standby in 48 ~ 50 DEG C of water-baths.Doubling dilution will be used in advance
Required concentration liquid prepared by method is sequentially added in nutrient agar by label, is configured to drug containing nutrient agar,
Inject in plate, it is standby after solidification.
Microbionation：It is seeded to and is prepared using 0.001 ~ 0.002 mL of the micro inoculator absorption bacterial suspensions configured
Drug containing nutrient agar surface.
2nd, the joint antibacterial experiment method of combined extracts sample：Micro broth dilution method
Using drug sensitive experiment is combined, using micro checkerboard method, research shrubby sophora extract is combined with tea extract
Bacteriostatic experiment effect, its FIC is established, specify the pattern of its interaction and the rational proportion of compatibility.
The preparation of nutrient broth medium：Nutrient broth medium is taken to add the ratio of 1L water to be matched somebody with somebody according to 18g culture mediums
System.Heating water bath is until culture medium is completely dissolved.By the culture medium after being completely dissolved, 9ml is taken as in test tube with pipette.
103.3Pa high pressure steam sterilizations 20-25min.After culture medium constant temp after sterilizing preserves 48-72h, no muddy produce can then make
With.
Fluid nutrient medium is inoculated with：By 2005 editions《Pharmacopeia》Annex XIII C bacteriums, mould and saccharomycete count bacterium solution system
It is standby：Bacterium to be measured is inoculated with into nutrient broth medium, cultivates 18 ~ 24 h.
Make bacterial suspension：By bacterium solution of the concentration equivalent to 0.5 Maxwell opacity tube, prepared with 0.9% aseptic sodium chloride solution
Into concentration equivalent to 5 × 105Cfu/ml~5 × 106The suspension of cfu/ml bacterium solution.
Combination is tested：Measure intends MIC of the united antibacterials to detection bacterium respectively first.With maximum drug concentration
2 times for MIC are maximum concentration, two-fold dilution.The dilution of two kinds of medicines is carried out in the file and row of square formation respectively, is so existed
Often manage two kinds of medicine mixed liquors that various concentrations combination is can obtain in (hole).Inoculated bacteria suspension, 35 DEG C are incubated observation knot after 18h
Fruit.Calculating section Mlc (FIC) index.
3rd, result of the test
Known in pharmaceutical field technical staff, typically, minimum inhibitory concentration (MIC) is in below 1mg/mL, it is believed that thing
The fungistatic effect of matter is affirmative, and when MIC is more than 10mg/mL, the fungistatic effect of material can not receive, or generally recognize
For no fungistatic effect.
For staphylococcus aureus, both shrubby sophora extract and green-tea extract, which are applied in combination, shows obvious synergy
Effect, the MIC difference of each material are as follows：The mg/mL of shrubby sophora extract MIC=0.025；The mg/mL of green-tea extract MIC=0.25；
Shrubby sophora extract+green-tea extract (1:2.5)=0.01mg/mL；Shrubby sophora extract+green-tea extract (1:2)=0.0125mg/
mL；Shrubby sophora extract+green-tea extract (1:3)=0.0125 mg/mL.
For Escherichia coli, both shrubby sophora extract and green-tea extract, which are applied in combination, shows obvious bacteriostasis,
The MIC difference of each material is as follows：Shrubby sophora extract exists>Obvious fungistatic effect is had not yet to see under 10mg/mL concentration；Green-tea extract
>Obvious fungistatic effect is had not yet to see under 20mg/mL concentration；Shrubby sophora extract+green-tea extract (1:2.5)=0.0125mg/
ML, show obvious fungistatic effect；Shrubby sophora extract+green-tea extract (1:2)=0.025mg/mL, obvious suppression is shown
Bacterium effect；Shrubby sophora extract+green-tea extract (1:3)=0.025 mg/mL, obvious fungistatic effect is shown.
The above-mentioned fungistatic effect for Escherichia coli both non-classical synergy, also non-classical synergistic effect, goes out people
Discovery shrubby sophora extract in expectation ground combines to use than independent extract with green-tea extract has significantly stronger effect.
Inventor is in the experiment of supplement, with reference to the method for preparation example 1, but respectively using 30%, 40%, 50%, 70%,
80% or 90% ethanol obtains 6 batches of shrubby sophora extracts and carries out fungistatic effect according to bacteriostatic test method above respectively as Extraction solvent
Test, as a result each shrubby sophora extract and green-tea extract are with 1:When 2.5 ratios combine, suppression is had not yet to see in the range of 10 ~ 40mg/mL
Bacterium acts on；Each shrubby sophora extract is with green-tea extract with 1:When 1 ratio combines, antibacterial work is had not yet to see in the range of 10 ~ 25mg/mL
With；Each shrubby sophora extract is with green-tea extract with 1:When 4 ratios combine, bacteriostasis is had not yet to see in the range of 10 ~ 20mg/mL.
Inventor with reference to the method for preparation example 1, but adjusts pH value in alkalinization respectively in the experiment of supplement
To 8,9,11,12, obtain 4 batches of shrubby sophora extracts and carry out fungistatic effect test according to bacteriostatic test method above respectively, as a result each hardship
Conopsea extraction is with green-tea extract with 1:When 2.5 ratios combine, bacteriostasis is had not yet to see in the range of 10 ~ 20mg/mL；Each kuh-seng
Extract is with green-tea extract with 1:When 1 ratio combines, bacteriostasis is had not yet to see in the range of 10 ~ 25mg/mL；Each kuh-seng extraction
Thing is with green-tea extract with 1:When 4 ratios combine, bacteriostasis is had not yet to see in the range of 10 ~ 50mg/mL.
3rd, Chinese medicine composition example part
Composition example 1：
By shrubby sophora extract and green-tea extract with 1:2.5 weight proportion is well mixed, and is ground into fine powder, with aluminium plastic bag bag
Powder, every bag of content weight 5g is made in dress.
Composition example 2：
By shrubby sophora extract and green-tea extract with 1:2 weight proportion is well mixed, and is ground into fine powder, adds 1 times of weight
Cornstarch, be well mixed, be adhesive wet granular with the 3%PVP K30 aqueous solution, dry, packed and be made with aluminium plastic bag
Granula, every bag of content weight 10g.
Composition example 3：
By shrubby sophora extract and green-tea extract with 1:3 weight proportion is well mixed, and is ground into fine powder, adds 1 times of weight
Cornstarch, be well mixed, be wetting agent wet granular with water, dry add 0.1 times of weight of cornstarch carboxymethyl form sediment
Powder sodium, it is well mixed, adds the magnesium stearate of 0.01 times of weight of cornstarch, is well mixed, tabletted, every piece weight
1.5g, tablet is made.
Claims (9)
1. a kind of Chinese medicine composition, including shrubby sophora extract and green-tea extract, and optionally pharmaceutically acceptable auxiliary
Material, wherein the weight of the shrubby sophora extract and green-tea extract ratio is 1：1-5.
2. Chinese medicine composition according to claim 1, wherein the shrubby sophora extract is prepared as follows：By hardship
Ginseng crush after with 60% ethanol be extract solution immersion, then heating and refluxing extraction 1-3 times；The extract solution of merging is concentrated into no alcohol
Watery hydrochloric acid is used after taste, until solution ph is 1-2, after filtering off insoluble matter, filtrate chloroform extraction, the aqueous phase of merging is used
Ammoniacal liquor adjusts pH value to 9-11, then with chloroform extraction, merges organic layer, evaporation of solvent, is dried to obtain kuh-seng and carries
Take thing.
3. Chinese medicine composition according to claim 1, wherein the shrubby sophora extract is prepared as follows：Kuh-seng
Powder is beaten, sieving, after soaking 2h as extract solution using 60% ethanol, heating and refluxing extraction 1.5h, after extracting 2 times, merges extract solution；Carry
After taking liquid to be concentrated into no alcohol taste, add 0.5% hydrochloric acid until solution ph be 1-2, after elimination insoluble matter, filtrate is with three chloromethanes
After alkane extracts 3 times, aqueous phase adjusts pH value with ammoniacal liquor and merges organic layer to 9.5-10.5, chloroform extraction 3 times, and evaporation removes
Solvent, it is dried to obtain shrubby sophora extract.
4. Chinese medicine composition according to claim 1, wherein the green-tea extract is prepared as follows：Will be green
Tea is soaked in water, then heating extraction, merges extract solution, concentration, dries, produce.
5. Chinese medicine composition according to claim 1, wherein the green-tea extract is prepared as follows：Will be green
Tea soaks 10-15 hours with 5-10 times of water measured, and then in 70~90 DEG C of heating extractions 1-3 times, each 1-3 hours, merging carries
Liquid is taken, is filtered, concentration, dries, produces.
6. Chinese medicine composition according to claim 1, wherein the pharmaceutically acceptable auxiliary material include selected from following one kind or
It is a variety of：Diluent, adhesive, disintegrant, lubricant.
7. Chinese medicine composition according to claim 1, it is in forms such as tablet, granule, powder, capsule, pills.
8. preparing the method for any one of the claim 1-7 Chinese medicine composition, it comprises the following steps：
(1) shrubby sophora extract is provided；
(2) green-tea extract is provided；
(3) shrubby sophora extract is mixed with the green-tea extract, wherein pharmaceutically acceptable auxiliary material is optionally added into, will
Gained mixture is prepared into appropriate form, produces, wherein the weight of the shrubby sophora extract and green-tea extract ratio is 1：1-
5。
9. purposes of any one of the claim 1-7 Chinese medicine compositions in preparing for antimicrobial product.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1239669A (en) * | 1999-03-24 | 1999-12-29 | 中国医学科学院肿瘤医院、肿瘤研究所 | Composition of Radix Sophorae Flavescentis and green tea and its application in treating condyloma |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1239669A (en) * | 1999-03-24 | 1999-12-29 | 中国医学科学院肿瘤医院、肿瘤研究所 | Composition of Radix Sophorae Flavescentis and green tea and its application in treating condyloma |
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