CN1233399A - Pesticide contg. nimbin and use therefor - Google Patents
Pesticide contg. nimbin and use therefor Download PDFInfo
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- CN1233399A CN1233399A CN 99114796 CN99114796A CN1233399A CN 1233399 A CN1233399 A CN 1233399A CN 99114796 CN99114796 CN 99114796 CN 99114796 A CN99114796 A CN 99114796A CN 1233399 A CN1233399 A CN 1233399A
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Abstract
The present invention relates fo an insecticide,its raw material contains 0.05%-95.00% nimbin and is diluted into sprays containing 0.05-1000ppm nimbin. It realizes the preventing and treating of pests through food repelling action of harmful insect and regulating the growth and proliferation of insect.
Description
The present invention relates to a kind of natural insecticide technical field.
Nimbin is from the Meliaceae plant, for example print the environment-friendly type natural insecticide of extract in the chinaberry (Azadirachta indica) efficient, low toxicity, low-residual,, non-environmental-pollution nontoxic to the person poultry safety, be one of current biological insecticides of praising highly the most, can be widely used in the control pest.Nimbin belongs to four and falls triterpene compound, and its ISO adopted name is Azadirachtin, and molecular formula is C35H44O16, and the CIPAC digital code for the 11141-17-6 chemical structural formula is:
Nimbin belongs to the broad spectrum activity vegetable insecticide, and its molecular structure complexity can't obtain by synthetic method at present, normally from the Meliaceae plant, as the seed of seal chinaberry, extracts acquisition.The commodity of external existing nimbin emulsion, domestic also do not have mature technique at present from the Meliaceae plant material, produces commercial nimbin insecticide as neem seed.Chinese invention patent application 96119630 discloses a kind of vegetalitas compound pesticide and production technology thereof, it is to adopt the chinaberry fruit, blade or bark, be equipped with Pericarpium Zanthoxyli fruit and the tea seed is prepared from, but relating to, it does not contain the nimbin pesticide product, do not relate to the bulk drug and the preparation that contain nimbin, also not relating to the nimbin is the production technology of standard, and effect test indoor, the field control harmful insect.
The present application people systematically separates and has identified the chemical constitution of 16 kinds of compounds from the seed of seal chinaberry, wherein the biologically active with nimbin is the highest.Inventor and in Chinese invention patent application 96112529.2, disclose the preparation technology of nimbin.
The purpose of this invention is to provide a kind of insecticide that contains nimbin, it realizes the control to different insects by growing, breed and regulate to the food refusal effect of harmful insect and to insect growth.
The present invention also provides the content of the nimbin in accurate mensuration plant material, bulk drug, the preparation, makes it to become the product that quality standard is arranged, and guarantees effective application.
Purpose of the present invention is achieved through the following technical solutions:
One, be raw material with the chinaberry tannin plant, as print the seed of chinaberry or plant benevolence, after the shelling, with organic solvent such as benzinum or after squeezing the grease degreasing, use organic solvent such as ethanol, methanol eddy to extract again, extract is after concentrating, distribute organic facies by chloroform, ethyl ester, reclaim organic solvent, extract is handled with benzinum, perhaps handles through adsorption chromatography again, obtains to be rich in the nimbin raw material medicine of nimbin 0.05%～95.00%, and with the content of high-pressure liquid chromatography nimbin, for the formulation of insecticides of preparing different needs and purposes further.
Two, contain the preparation of the formulation of insecticides of nimbin: according to different purposes and purpose, get the different bulk drug of above-mentioned nimbin content, earlier with 2～50 times 95% dissolve with ethanol, add emulsifier such as tween, glycerine, an amount of dimethylbenzene again, add an amount of water and be mixed with 0.001%～90.00% preparation, and demarcate with the standard sample of nimbin.
Three, described preparation can be diluted to the spray agent that contains nimbin 0.05～1000ppm concentration; The bulk drug that contains nimbin can be mixed with emulsion, aqua, colloidal suspending agent, soluble powder, wetting powder.
Four, high-pressure liquid phase chromatogram therapy determining nimbin content
It is the reversed-phase column RP-18 of standard that the present invention has formulated in plant material, bulk drug and the preparation with the nimbin, with the acetonitrile/water is the assay method of flowing phase: fixing phase RP-18, the flowing phase acetonitrile/water, flow velocity 0.8ml/min, make external standard with nimbin, be mixed with the solution of known content, with this understanding, the nimbin in plant material, bulk drug and the preparation all can reach baseline separation.This assay method has guaranteed the quality of plant material, bulk drug, preparation preparation, helps the monitoring in the production process, for suitability for industrialized production and apply convenience is provided.
Five, the insecticidal effect and the production application thereof of following indoor and field desinsection drug effect explanation nimbin preparation with the preparation that contains nimbin.
Indoor insect toxicological test
Embodiment 1: the bulk drug that contains 91.72% nimbin with the present invention's preparation, be mixed with the preparation that contains nimbin 90%, and water is by doubly being diluted to the test solution drop cabbage caterpillar in 3 age that contains nimbin 1ppm concentration, and lethality reaches 90% after 18 days; Drop cabbage caterpillar food refusal in 4 age effect reaches 94.2%.
Embodiment 2: will contain 0.5% nimbin preparation water and be diluted to 800 times respectively, 600 times, 400 times, 300 times, making five concentration for 150 times handles, with blank, for the examination pest species be three age diamond-back moth (Plutellaxylstella L.) larva and three age the cabbage caterpillar larva, the broccoli leaf is edible for insect, adopt blade dipping and spray testing method, investigated back 1 day, 3 days, 7 days, 10 days, 15 days, 20 days larval mortality, larva to survival goes ahead with one's investigation that it is pupated, sprout wings, the egg laying amount of adult post-coitum and ovum hatching situation etc., the result proves that 0.5% nimbin preparation has certain food refusal effect to diamond-back moth and imported cabbageworm (Piers rapae L.) larva, reduce food ingestion, cause larva to postpone to cast off a skin, the deformity of casting off a skin is to dead, each concentration of treatment 15～20 days, diamondback moth larvae lethality 60%～90%; Cabbage caterpillar lethality 96%～100%.This preparation is handled the back to diamondback moth larvae and cabbage caterpillar influence pupation rate and eclosion rate, and not only egg laying amount significantly reduces, and lay eggs all can not ovumization, diamond-back moth, cabbage caterpillar are had tangible virulence effect.
Embodiment 3: contain the toxicity of the preparation of 45% nimbin to liriomyza bryoniae
Liriomyza bryoniae is to crops, harm as vegetables, flowers, legume crop etc. is serious, has north expanded from southern area in China, does not also have the prophylactico-therapeutic measures of special efficacy at present both at home and abroad, the preparation that contains 45% nimbin has preventive and therapeutic effect through the indoor and outdoor test to liriomyza bryoniae.
The preparation that will contain 45% nimbin is diluted with water to the spray agent that contains nimbin 25ppm concentration, is material with the broad bean, south american leaf miner is had significant food refusal effect, and lethality 31.2 in 24 hours, is higher than contrast 0% significantly.
Embodiment 4: contain the toxicological effect of the preparation of 45% nimbin to big white butterfly
The preparation that will contain 45% nimbin is diluted with water to the solution that contains 0.5% nimbin, be diluted with water to again during use and contain nimbin 50,125,250,500, the concentration of 1000ppm is observed the toxicological effect of the large white butterfly Nepal subspecies (Pieris brassicae neplensis) of picking up from the field, the concentration of 50ppm reduces the food ingestion that occurs in 3～4 rheological processes, slow in action, food ingestion is littler during to 4 ages, almost motionless, at last all in this death in length of time, its main cause is the effect that has influenced the moulting hormone in the polypide, causes larva not cast off a skin and death.
Field trial is to the drug effect of harmful insect
Embodiment 5: the field efficacy of control cabbage caterpillar
To contain 0.5% nimbin preparation water and be diluted to 150 times respectively, 300 times, 400 times, making concentration for 600 times handles, with blank, the object crop is a cauliflower, dispenser period is that cabbage caterpillar takes place to contain the phase for 3 ages, thinner evenly is sprayed on the cauliflower plant, the sampling of employing five-point type, the every strain worm radix of investigation before the dispenser, after the dispenser 3 days, 7 days, 10 days, respectively investigate the borer population alive in every strain after 15 days, calculate insect population go down rate and correcting controling effect then, drug effect result shows: contain after 150 times of dispensers of 0.5% nimbin preparation 3 days, 7 days, 10 days, proofreaied and correct preventive effect in 15 days between 12.6%～70.2%; Proofreaied and correct preventive effect between 6.1%～67.6% in 3 days, 7 days, 10 days, 15 days after 300 times of dispensers; Proofreaied and correct preventive effect between 5.4%～56.0% in 3 days, 7 days, 10 days, 15 days after 400 times of dispensers; After 600 times of dispensers 3 days, 7 days, 10 big, proofreaied and correct preventive effect in 15 days between 2.6%～37.3%; 4 concentration of treatment preventive effects of 0.5% nimbin preparation all increase with passage of time behind the medicine, and wherein 150 times, 300 times increase amplitudes are bigger, and the preventive effect performance behind medicine 10 days, 15 days the time better.
Embodiment 6: the field efficacy of control diamond-back moth
0.5% nimbin preparation water is diluted to 100 times respectively, 200 times, 300 times, making concentration for 600 times handles, with blank, the object crop is a cauliflower, dispenser is that diamond-back moth takes place to contain the phase for 2～3 ages period, thinner evenly is sprayed on the cauliflower plant, the sampling of employing five-point type, the every strain worm radix of investigation before the dispenser, after the dispenser 3 days, 7 days, 10 days, respectively investigate the borer population alive in every strain after 15 days, calculate insect population go down rate and correcting controling effect then, drug effect result shows: contain after 100 times of dispensers of 0.5% nimbin preparation 3 days, 7 days, 10 days, proofreaied and correct preventive effect in 15 days between 71.0%～88.4%; 3 days, 7 days, 10 days, 15 days correction preventive effects are between 57.5%～80.7% after diluting 200 times of dispensers; 3 days, 7 days, 10 days, 15 days correction preventive effects are between 54.8%～75.1% after diluting 300 times of dispensers; Proofreaied and correct preventive effect between 44.3%～57.5% in 3 days, 7 days, 10 days, 15 days after 600 times of dispensers; And control experiment district insect population is in the growth situation always, 100 times in 0.5% nimbin preparation all had higher preventive effect to diamond-back moth in 3 days, 5 days, 7 days, 10 days behind medicine, after diluting 200 times, 300 times, though the 3rd day preventive effect is lower behind medicine, but had higher preventive effect in 5 days, 7 days, 10 days behind the medicine, dilute 600 times, 3 days, 5 days, 7 days, 10 days preventive effects are lower behind the medicine, in general, 100 times, 200 times, 300 times of 0.5% nimbin preparation dilutions have higher control efficiency to diamond-back moth.
The advantage that the present invention has or good effect: because the nimbin preparation is to Antifeedant Effects and the life of harmful insect Long hair educates, breed to have and suppress and regulating action, and it is the crops that produce pollution-free source, as vegetables, fruit, The important biomolecule pesticide of the crop such as flowers, grain. The comparative chemistry agricultural chemicals, the general quick-acting of biological pesticide Relatively poor, because mechanism of action difference, its drug effect often obviously strengthens with the time behind the medicine, from 4 concentration ratios of test , 0.5% nimbin through 150 times, 300 times cabbage caterpillar is had preferably prevention effect, dispenser preferably rests in The low instar larvae phase.
Claims (4)
1, a kind of insecticide that contains nimbin extracts from the Meliaceae plant, it is characterized in that: contain 0.05%～95.00% nimbin in the bulk drug of extraction.
2, insecticide according to claim 1 is characterized in that: bulk drug is mixed with the preparation that contains 0.001%～90.00% nimbin.
3, insecticide according to claim 2 is characterized in that: preparation is diluted to the spray agent that contains nimbin 0.05～1000ppm concentration.
4, insecticide according to claim 2 is characterized in that: the bulk drug that contains nimbin can be mixed with emulsion, aqua, colloidal suspending agent, soluble powder, wetting powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99114796 CN1233399A (en) | 1999-04-14 | 1999-04-14 | Pesticide contg. nimbin and use therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99114796 CN1233399A (en) | 1999-04-14 | 1999-04-14 | Pesticide contg. nimbin and use therefor |
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| Publication Number | Publication Date |
|---|---|
| CN1233399A true CN1233399A (en) | 1999-11-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 99114796 Pending CN1233399A (en) | 1999-04-14 | 1999-04-14 | Pesticide contg. nimbin and use therefor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112106C (en) * | 2000-04-27 | 2003-06-25 | 华南农业大学 | Plant-type nimbin insecticide |
| CN102007925A (en) * | 2010-12-10 | 2011-04-13 | 明光市天骄科技发展有限公司 | Azadirachtin-attapulgite compound pesticidal granule |
| CN104094980A (en) * | 2014-08-06 | 2014-10-15 | 辽宁绿禾农业有限公司 | Azadirachtin water aqua for treating Chinese chive root maggot and preparation method thereof |
| CN106900756A (en) * | 2017-04-11 | 2017-06-30 | 四川农业大学 | Insecticidal composition of pueraria peduncularis extract and azadirachtin for preventing and treating agricultural pests and application thereof |
-
1999
- 1999-04-14 CN CN 99114796 patent/CN1233399A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112106C (en) * | 2000-04-27 | 2003-06-25 | 华南农业大学 | Plant-type nimbin insecticide |
| CN102007925A (en) * | 2010-12-10 | 2011-04-13 | 明光市天骄科技发展有限公司 | Azadirachtin-attapulgite compound pesticidal granule |
| CN102007925B (en) * | 2010-12-10 | 2013-05-29 | 明光市天骄科技发展有限公司 | Azadirachtin-attapulgite compound pesticidal granule |
| CN104094980A (en) * | 2014-08-06 | 2014-10-15 | 辽宁绿禾农业有限公司 | Azadirachtin water aqua for treating Chinese chive root maggot and preparation method thereof |
| CN106900756A (en) * | 2017-04-11 | 2017-06-30 | 四川农业大学 | Insecticidal composition of pueraria peduncularis extract and azadirachtin for preventing and treating agricultural pests and application thereof |
| CN106900756B (en) * | 2017-04-11 | 2019-06-11 | 四川农业大学 | Insecticidal composition of pueraria peduncularis extract and azadirachtin for preventing and treating agricultural pests and application thereof |
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