CN1229086A - Method for preparing rare ginsengoside using enzymatic method to modify ginsenoside glycoside - Google Patents
Method for preparing rare ginsengoside using enzymatic method to modify ginsenoside glycoside Download PDFInfo
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- CN1229086A CN1229086A CN99112764A CN99112764A CN1229086A CN 1229086 A CN1229086 A CN 1229086A CN 99112764 A CN99112764 A CN 99112764A CN 99112764 A CN99112764 A CN 99112764A CN 1229086 A CN1229086 A CN 1229086A
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- saponin
- ginsenoside
- glycosyl
- scarce
- enzyme
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- 229930182494 ginsenoside Natural products 0.000 title claims description 29
- 229940089161 ginsenoside Drugs 0.000 title claims description 29
- 238000000034 method Methods 0.000 title claims description 18
- 238000006911 enzymatic reaction Methods 0.000 title 1
- 229930182470 glycoside Natural products 0.000 title 1
- 150000002338 glycosides Chemical class 0.000 title 1
- 229930182490 saponin Natural products 0.000 claims abstract description 64
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims abstract description 62
- 150000007949 saponins Chemical class 0.000 claims abstract description 49
- 125000003147 glycosyl group Chemical group 0.000 claims abstract description 14
- 235000008434 ginseng Nutrition 0.000 claims abstract description 13
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 11
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 11
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 5
- 108090000790 Enzymes Proteins 0.000 claims description 23
- 102000004190 Enzymes Human genes 0.000 claims description 23
- -1 β-glucose saponin Chemical class 0.000 claims description 13
- 240000004371 Panax ginseng Species 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 150000008131 glucosides Chemical group 0.000 claims description 4
- 235000002789 Panax ginseng Nutrition 0.000 claims description 3
- CKUVNOCSBYYHIS-IRFFNABBSA-N (20S)-ginsenoside Rh2 Chemical compound O([C@H]1CC[C@]2(C)[C@H]3C[C@@H](O)[C@H]4[C@@]([C@@]3(CC[C@H]2C1(C)C)C)(C)CC[C@@H]4[C@@](C)(O)CCC=C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O CKUVNOCSBYYHIS-IRFFNABBSA-N 0.000 claims description 2
- 241000221198 Basidiomycota Species 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 241001655322 Streptomycetales Species 0.000 claims description 2
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 2
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 2
- 108010047754 beta-Glucosidase Proteins 0.000 claims description 2
- 102000006995 beta-Glucosidase Human genes 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- RAQNTCRNSXYLAH-RFCGZQMISA-N (20S)-ginsenoside Rh1 Chemical compound O([C@@H]1[C@H]2C(C)(C)[C@@H](O)CC[C@]2(C)[C@H]2C[C@@H](O)[C@H]3[C@@]([C@@]2(C1)C)(C)CC[C@@H]3[C@@](C)(O)CCC=C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RAQNTCRNSXYLAH-RFCGZQMISA-N 0.000 claims 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims 1
- 235000017709 saponins Nutrition 0.000 abstract description 56
- 241000208340 Araliaceae Species 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 26
- 229940088598 enzyme Drugs 0.000 description 18
- 235000019441 ethanol Nutrition 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 230000001093 anti-cancer Effects 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 239000007974 sodium acetate buffer Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241000228232 Aspergillus tubingensis Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- WQLVFSAGQJTQCK-UHFFFAOYSA-N diosgenin Natural products CC1C(C2(CCC3C4(C)CCC(O)CC4=CCC3C2C2)C)C2OC11CCC(C)CO1 WQLVFSAGQJTQCK-UHFFFAOYSA-N 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- NWMIYTWHUDFRPL-UHFFFAOYSA-N sapogenin Natural products COC(=O)C1(CO)C(O)CCC2(C)C1CCC3(C)C2CC=C4C5C(C)(O)C(C)CCC5(CCC34C)C(=O)O NWMIYTWHUDFRPL-UHFFFAOYSA-N 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 235000015961 tonic Nutrition 0.000 description 2
- 230000001256 tonic effect Effects 0.000 description 2
- CKUVNOCSBYYHIS-UHFFFAOYSA-N (20R)-ginsenoside Rg3 Natural products CC(C)=CCCC(C)(O)C1CCC(C2(CCC3C4(C)C)C)(C)C1C(O)CC2C3(C)CCC4OC1OC(CO)C(O)C(O)C1O CKUVNOCSBYYHIS-UHFFFAOYSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical class C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- CKUVNOCSBYYHIS-LGYUXIIVSA-N 20(R)-Ginsenoside Rh2 Natural products O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@H]1C(C)(C)[C@H]2[C@@](C)([C@H]3[C@](C)([C@@]4(C)[C@H]([C@H](O)C3)[C@@H]([C@](O)(CC/C=C(\C)/C)C)CC4)CC2)CC1 CKUVNOCSBYYHIS-LGYUXIIVSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 101100140896 Caenorhabditis elegans rgs-2 gene Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 235000005769 Japanese ginseng Nutrition 0.000 description 1
- 241000795633 Olea <sea slug> Species 0.000 description 1
- 235000002791 Panax Nutrition 0.000 description 1
- 241000208343 Panax Species 0.000 description 1
- 241000168720 Panax japonicus Species 0.000 description 1
- 235000003174 Panax japonicus Nutrition 0.000 description 1
- 241000180649 Panax notoginseng Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 125000000188 beta-D-glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000003544 deproteinization Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229940059442 hemicellulase Drugs 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229960001866 silicon dioxide Drugs 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/58—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/308—Foods, ingredients or supplements having a functional effect on health having an effect on cancer prevention
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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Abstract
An enzymolysis process for preparing rare ginseng saponin by modifying the glycosyl of ginseng saponin hydrolyzing the ginseng saponins with the saponinases including beta-glucosidease, alpha- or beta-arabinosylase, alpha-rhamnosidease, etc to hydrolyze out part of glycosyl radicals on moleculae of saponin, resulting in new rare saponin. Its advantages are low cost and high economic value.
Description
The present invention relates to a kind of biological method that changes the ginsenoside glycosyl, particularly adopt enzyme process to change the method that the ginsenoside glycosyl prepares scarce ginsenoside.
Genseng is that the genseng (Panax) in the plant Araliaceae (araliaceae) belongs to, four not of the same race and other mutation are arranged, as genseng (P.ginseng), Radix Panacis Quinquefolii (P.quenquefolinus), pseudo-ginseng ginseng (P.notoginseng), rhizome of Japanese Ginseng (P.uaponicus) etc. and some other mutation.Genseng is the valuable tonic of the strengthening by means of tonics that has won fame both at home and abroad, and the effective constituent that mainly contains of genseng is a saponin.The ginsenoside of having found has 34 kinds; Ginsenoside can be divided into three kinds according to the difference of sapogenin, i.e. glycols saponin class (protopanaxdill-type Gisenosides), triol saponin class (Protopanaxtriol-typeGisenosides).Wherein the glycols saponin accounts for 50～60% of whole saponin, secondly is three alcohols saponins 30～35%, also has olea acids saponin.The glycols saponin Ra1, Ra2, Ra3, RB1, Rb2, Rb3, Rc, Rd and the Rg3 that account for saponin total amount 50～60% in the genseng are identical with scarce ginsenoside Rh2 sapogenin, and just sugar chain is different.Wherein saponin content height in genseng such as Rb, Rc, Rd obtain easily.But the Rh2 saponin that antitumour activity is very high exists in white ginseng hardly, only contain 100,000 at red ginseng/.On glucoside unit the 3rd carbon of Rh2 and R6, Rc, the same saponin molecule of Rd a β-glucosyl group is arranged, therefore, can obtain being worth high anticancer saponin Rh2 as long as the part glycosyl on the saponin molecules such as Rb, Rc, Rd is removed.Equally,, remove the part glycosyl, can obtain being worth high saponin Rh1 for accounting for saponin total amount 40～50% panoxatriol class saponin Re, Rf, Rg1 and Rg2 saponin in the genseng.
The present invention seeks to: utilize biotechnology, provide a kind of enzyme process to change the method that the ginsenoside glycosyl prepares scarce ginsenoside.
The present invention is achieved in that enzyme process changes the method that the ginsenoside glycosyl prepares scarce ginsenoside, it is characterized in that: adopt saponin beta-glucosidase, α-, saponin enzymes such as β-arabinofuranosidase/xylosidase, α-mouse season Glycosylase, β-glycuronide enzyme or beta-galactosidase enzymes, for the processing that is hydrolyzed of the ginsenoside of all kinds of Panax ginseng, can make the part glycosyl in the saponin molecule glucoside unit be hydrolyzed, become new rare saponin.
Realize when of the present invention that with such scheme described various saponin enzymes can be extracted by microorganism such as bacterium, streptomycete, mould, yeast, basidiomycetes or plant, plant seed processing.
Use the present invention, can β-glucose saponin ginsenoside enzymatically hydrolyzing Rg
3, obtain scarce ginsenoside Rh
2Also can α-Ji sugar saponin ginsenoside enzymatically hydrolyzing Rg
2, obtain scarce ginsenoside Rh
1Also can
Can produce the saponin enzyme and directly be inoculated on ginseng powder or the sheet and cultivate, obtain high ginseng powder of scarce ginsenoside component content or sheet.
Core of the present invention is to utilize the part glycosyl of the higher ginsenoside of saponin enzyme hydrolysis content in each seed ginseng, thereby obtains scarce ginsenoside.In fact, the general cellulase or the excision enzyme of hemicellulase are difficult to hydrolysis saponin glycosyl, because saponin is different with Mierocrystalline cellulose, the glucoside unit ring with big has only saponin enzyme could realize hydrolysis saponin glycosyl.
The invention has the advantages that: it can allow people in simple and economic mode, and some ginsenoside compositions " transformation " that occurring in nature is extensively existed become Rh1, Rh2 and so on ginsenoside composition rare, that economic worth is very high again.
Below in conjunction with embodiment the present invention is done further narration:
Embodiment 1:
Bacillus sp.JF bacterium with high-temperature aerobic, in the 0.01MgSO4 substratum that contains 3% Semen Maydis powder, 1% genseng extract, temperature is ventilated down for 60 ℃ and was cultivated 30～40 hours, then bactofugation, 70% ethanol sedimentation zymoprotein, collect albumen, freeze-drying, obtain can hydrolysis the beta-glucoside saponin enzyme of β-glucose key.
Get the 3 above-mentioned saponin enzymes of gram and 140 milliliters of physiological waters mixing; 10 gram panaxoside Rg 3,20 ml physiological salines and 40 milliliters of ethanol mix.Two kinds of above-mentioned mixed solution remix were 60 ℃ of following stirring reactions of temperature 12 hours.The reaction back adds 800 milliliters of ethanol, removes by filter albumen precipitation, and excess ethyl alcohol is washed precipitation, filtrate decompression evaporate to dryness, and dry product distilled water flush away salt three times deducts evaporate to dryness, obtains 7 grams and contains 40～95%Rh2 saponin.
Embodiment 2:
Degerming after aspergillus tubigensis is cultivated, nutrient solution ethanol sedimentation zymoprotein with the 0.02M sodium-acetate buffer dissolving of pH5, separates sugared saponin enzyme of α-mouse season, freeze-drying with DEAE-Cullulose ion exchange resin salt gradient elution method.
10 grams, three alcohols ginsenoside Res are dissolved in 200 milliliters of 0.02M sodium-acetate buffer dissolvings with PH5, add 0.05 gram α-mouse season sugar saponin enzyme 40 ℃ of stirring reactions of temperature 12 hours, be heated to 100 ℃ 30 minutes, filter out the Deproteinization precipitation, macroporous resin 200 gram AB-8 absorption, 500 milliliter of 70% ethanol elution, its ethanol liquid evaporated under reduced pressure gets thick Rg1 saponin.Its thick Rg1 saponin separates through the silicagel column partition method, and underpressure distillation gets 5.5 gram Rg1.
Same quadrat method, above-mentioned enzyme are handled 10 gram triol panaxoside Rgs 2, can obtain valuable pH1 saponin 4.5～6 grams.
Embodiment 3:
Cultivate the back degerming after getting genus bacillus, use the ethanol sedimentation zymoprotein, freeze-drying.10***** is restrained genseng mixing saponin be dissolved in 200 milliliters, add the above-mentioned mixing saponin enzyme of 0.3 gram, 40 ℃ of stirring reactions of temperature 12 hours with the dissolving of the 0.02M sodium-acetate buffers of pH5.Through efficient liquid phase chromatographic analysis, 80% above saponin loses a glycosyl at least.Rare anticancer saponin Rh2, RH1 saponin content have improved hundred times.Reaction solution is through adding the heat extraction zymoprotein, and through 200 gram AB-8 porous resin absorption, 500 millis, 90% ethanol elution, evaporated under reduced pressure can obtain the high mixing saponin of the 8 rare saponin contents of gram.
Embodiment 4
The bacterium aspergillus tubigensis (not producing poison or harmful thing) of producing the saponin enzyme is inoculated on the ginseng tablet of moisture content 25～150%, cultivated 2～7 days, be drying to obtain the genseng that height contains rare saponin at 28～38 ℃.Through efficient liquid phase chromatographic analysis, 80% above saponin loses a glycosyl at least.Rare anticancer saponin RH2, RH1 saponin content have improved hundred times.
Claims (5)
1, enzyme process changes the method that the ginsenoside glycosyl prepares scarce ginsenoside, it is characterized in that: adopt saponin beta-glucosidase, α-, saponin enzymes such as β-arabinofuranosidase/xylosidase, α-mouse season Glycosylase, β-glycuronide enzyme or beta-galactosidase enzymes, for the processing that is hydrolyzed of the ginsenoside of all kinds of Panax ginseng, can make the part glycosyl in the saponin molecule glucoside unit be hydrolyzed, become new rare saponin.
2, method according to claim 1 is characterized in that: described various saponin enzymes can be extracted by microorganism such as bacterium, streptomycete, mould, yeast, basidiomycetes or plant, plant seed processing.
3, method according to claim 1 is characterized in that: with β-glucose saponin ginsenoside enzymatically hydrolyzing Rg
3, get scarce ginsenoside Rh
2
4, method according to claim 1 is characterized in that: with α-Ji sugar saponin ginsenoside enzymatically hydrolyzing Rg
2, get scarce ginsenoside Rh
1
5, method according to claim 2 is characterized in that: the mushroom that can produce the saponin enzyme directly is inoculated on ginseng powder or the sheet to be cultivated, and obtains high ginseng powder of scarce ginsenoside component content or sheet.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112764A CN1105781C (en) | 1999-03-17 | 1999-03-17 | Method for preparing rare ginsengoside using enzymatic method to modify ginsenoside glycoside |
| KR1019990045180A KR100329259B1 (en) | 1999-03-17 | 1999-10-14 | manufacturing process of ginseng saponins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112764A CN1105781C (en) | 1999-03-17 | 1999-03-17 | Method for preparing rare ginsengoside using enzymatic method to modify ginsenoside glycoside |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1229086A true CN1229086A (en) | 1999-09-22 |
| CN1105781C CN1105781C (en) | 2003-04-16 |
Family
ID=5276029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99112764A Expired - Fee Related CN1105781C (en) | 1999-03-17 | 1999-03-17 | Method for preparing rare ginsengoside using enzymatic method to modify ginsenoside glycoside |
Country Status (2)
| Country | Link |
|---|---|
| KR (1) | KR100329259B1 (en) |
| CN (1) | CN1105781C (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002053722A1 (en) * | 2000-12-29 | 2002-07-11 | Fengxie Jin | Ginsenoside glycosidase which hydrolyzes ginsenoside glycosyl and the use thereof |
| CN1305479C (en) * | 2003-09-28 | 2007-03-21 | 中国科学院大连化学物理研究所 | Low polarity ginsenoside combination possessing anti-tumor activity |
| WO2007035723A3 (en) * | 2005-09-19 | 2007-07-12 | Herbalscience Singapore Pte Ltd | Compositions and methods comprising panax species |
| CN102154428A (en) * | 2011-01-13 | 2011-08-17 | 佟心 | Method for preparing ginsenoside Rh2 |
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| GB2179042B (en) * | 1985-07-22 | 1988-11-23 | Takeda Chemical Industries Ltd | A process of producing ginsenoside-rd |
| CN1048283C (en) * | 1996-03-12 | 2000-01-12 | 金凤燮 | Method for extracting asponin composition from traditional Chinese medicinal material with enzymic processing |
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Also Published As
| Publication number | Publication date |
|---|---|
| KR100329259B1 (en) | 2002-03-20 |
| CN1105781C (en) | 2003-04-16 |
| KR20000062140A (en) | 2000-10-25 |
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