CN1228334A - Yanminling syrup and its preparing technology - Google Patents
Yanminling syrup and its preparing technology Download PDFInfo
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- CN1228334A CN1228334A CN 98122223 CN98122223A CN1228334A CN 1228334 A CN1228334 A CN 1228334A CN 98122223 CN98122223 CN 98122223 CN 98122223 A CN98122223 A CN 98122223A CN 1228334 A CN1228334 A CN 1228334A
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Abstract
The Yanminling syrup capable of completely raising immunological function, eliminating allergic reaction factor, controlling special infection and raising cure rate after endoscopic operation of functional nasal sinuses is made up by using the Chinese medicinal materials of leather leaf machonia stem, barbat skullcap, trichosanthes root, pueraria root, polyporus, astragalus root, tribulus fruit, gleditsia fruit, ligusticum root, magnolia flower, mint and licorice through a certain preparation process.
Description
The present invention relates to a kind of Yanminling syrup and manufacturing process thereof, uses after mainly peeping sharp operation on nasal sinus in functional.
Chronic sinusitis, polypous etiology are very complicated, and the participation of patient's part and general immunity situation, allergy factor, the problem of special infection, anatomical abnormalities or the like all have close dependency with typing (by stages) and cure rate.The success of operation on nasal sinus has only solved anatomical unusual ventilation drainage problem that reaches nasal sinuses under the endoscope, in addition the problems referred to above also need rely on postoperative with processing anti-, the art chamber, the more important thing is local and the raising of general immunity function, the elimination of allergy factor and the control of special infection.
The object of the present invention is to provide a kind of Yanminling syrup and manufacturing process thereof that can comprehensively improve immunologic function, elimination allergy factor, the special infection of control.
Purpose of the present invention can reach by following measure: the present invention makes 250 kilograms of syrup needs Mahonia fortunei (Lindl.) Fedde 29.5-30.5 kilogram, Herba Scutellariae Barbatae 29.5-30.5 kilogram, pollen 29.5-30.5 kilogram, Radix Puerariae 29.5-30.5 kilogram, Polyporus 29.5-30.5 kilogram, Radix Astragali 29.5-30.5 kilogram, Fructus Atriplicis Sibiricae 14.5-15.5 kilogram, Fructus Gleditsia 8.5-9.5 kilogram, Rhizoma Chuanxiong 14.5-15.5 kilogram, Flos Magnoliae 14.5-15.5 kilogram, 7.8 kilograms of thin He, Radix Glycyrrhizae 5.5-6.5 kilogram.With the distillation of Rhizoma Chuanxiong, Flos Magnoliae water, collect distillate, again with Rhizoma Chuanxiong, Flos Magnoliae medicinal residues with ten big functions, Herba Scutellariae Barbatae, pollen, Radix Puerariae, Polyporus, the Radix Astragali, Fructus Atriplicis Sibiricae, Fructus Gleditsia, Herba Menthae, Radix Glycyrrhizae, decoct with water twice, each two hours, collecting decoction filtered, filtrate is concentrated in right amount, add 65% alcohol precipitation 24 hours, and got supernatant, recovered alcohol, the distillate of gained medicinal liquid and Rhizoma Chuanxiong, Flos Magnoliae merges, and adds an amount of distilled water and simple syrup promptly.
Purpose of the present invention can also reach by following measure: described ten desirable 30 kilograms of big function labor, 30 kilograms of Herba Scutellariae Barbataes, 30 kilograms of pollen, 30 kilograms of Radix Puerariaes, 30 kilograms of Polyporus, 15 kilograms of Rhizoma Chuanxiongs, 15 kilograms of Flos Magnoliae, 7.5 kilograms of Herba Menthaes, 6 kilograms in Radix Glycyrrhizae.
The present invention has following advantage compared to existing technology:
There are some researches show: as the principal agent Radix Astragali in " Yanminling series " side, behind the clothes, IgM, IgE, IgG, IgA all have in the blood plasma increases or significantly increase (P＜0.05-0.01).The immunity of Polyporus pair cell has significant potentiation, and experiment shows: normal person's intramuscular injection " 757 " (Polyporus injection) 10 days, T lymphocyte transformation just significantly increase (P＜0.01).Rhizoma Chuanxiong has obviously antiallergic, anti-angiogenic inflammatory effect; Rhizoma Chuanxiong can suppress the platelet release function, alleviates vascular inflammation reaction and asthma attack degree and persistent period.Radix Trichosanthis is a kind of immunostimulant, and lymphocyte and hematid immunity function are risen comprehensively, and total complement IgG, IgA content are risen.The scholar Mcgrath of the U.S. etc., in reported first in 1989 Radix Trichosanthis since external inhibitory action to human immune deficiency, viral HIV-1, this medicine is subjected to the whole world and pays close attention to greatly.The blood stasis dispelling but Rhizoma Paridis, Mahonia fortunei (Lindl.) Fedde, Herba Scutellariae Barbatae, Radix Puerariae heat-clearing and toxic substances removing, Fructus Atriplicis Sibiricae, Spina Gleditsiae can be assaulted fortified position, Flos Magnoliae can be led to a surname's nose key.Form one and improved immunologic function comprehensively, eliminated the prescription of allergy factor, the special infection of control.The external nasal spray of forming by the medicines such as Aloe, Herba Centipedae, Moschus of awakening consciousness, detumescence toxin expelling in addition, a treating both the exterior and interior at the same time, giving consideration to both the incidental and fundamental, oral external mutual aid have been formed, the comparatively complete Therapeutic Method of not only getting rid of evils but also setting upright, thereby after functional nasal endoscope operation, improve cure rate and played positive effect.
Its effect sees Yanminling syrup and the report of doing in following Guangdong Pharmaceutical University scientific research place of Yanminling nasal spray pharmacodynamic experiment for details:
One, gets given the test agent Yanminling oral agents concentrated extract (10g crude drug/ml) and Yanminling nasal spray (0.8g crude drug/ml).And Kunming mouse, male and female dual-purpose, body weight 18-22g; The SD rat, male and female dual-purpose, body weight 770-210g.
Two, method and result
The hexichol first is caused the inhibitory action of mice ear
Get 60 of mices, male and female half and half, be divided into 6 groups at random, it is matched group, the aspirin group, Yanminling oral agents (high dose group, low dose group), Yanminling nasal spray (high dose group, low dose group), oral administration 3 days, matched group is given the normal saline with volume, 30min after last administration in the 3rd day, cause inflammation outside being applied to 0.05ml dimethylbenzene in each mouse right ear, put to death animal behind the 1h, clip left and right sides auricle, getting the ears sheet with diameter 9mm card punch weighs respectively, about the difference of two auricle weight be the swelling degree, the result shows (table 1), Yanminling oral agents and Yanminling nasal spray xylol cause mice ear the obvious suppression effect.Table 1. Yanminling oral agents and Yanminling nasal spray are right
The inhibitory action of mice caused by dimethylbenzene xylene ear swelling
Poor (mg) matched group 21.0 ± 2.9 aspirin 0.1 8.9 ± 2.4** Yanminling oral agents 20 13.6 ± 3.3** of dosage (g/kg) left and right sides ear weight
10 15.9 ± 3.6* Yanminling nasal spray, 20 14.7 ± 3.5**
10 18.1 ± 3.2***p＜0.01, compare with matched group * p＜0.05
Ovum Gallus domesticus album is caused the inhibitory action of rat paw edema
60 of rats, male and female half and half random packet is the same, successive administration 7 days, with capillary tube measurement by magnification method survey respectively cause scorching before and after the right back sufficient sole of the foot volume of 30min and 1,3,6h, obtain its swelling rate by following formula, swelling rate (%)=(Vt-Vn)/Vn, Vn wherein, Vt represents respectively and causes the sufficient sole of the foot volumetric values in scorching front and back, rat is 30min after the last administration, 100% fresh Ovum Gallus domesticus album (0.1ml/ only) is caused inflammation in right back sufficient plantar subcutaneous injection, and the result shows (table 2), and Yanminling oral agents and Yanminling nasal spray cause rat paw edema to Ovum Gallus domesticus album the obvious suppression effect. table 2. Yanminling oral agents and Yanminling nasal spray can be taken charge of woods 0.1 28.6 ± 12.3** 30.7 ± 11.2** 29.9 ± 10.2** 31.4 ± 12.8** Yanminling oral agents 20 25.3 ± 10.8** 36.9 ± 13.4** 33.8 ± 12.3** 36.2 ± 10.3** to inhibitory action pedal swelling rate (%) group dosage (g/kg) the 30min 1h 3h 6h control group 45.9 ± 12.4 60.6 ± 13.9 58.9 ± 11.9 59.4 ± 13.8 that egg white causes rat paw edema
10 34.3 ± 11.1**, 42.5 ± 15.8**, 40.6 ± 10.7**, 39.7 ± 11.7** Yanminling nasal spray, 20 30.7 ± 10.5**, 35.5 ± 13.2**, 35.2 ± 12.4**, 36.3 ± 13.3**
10 33.4 ± 14.8*, 47.5 ± 12.1*, 44.9 ± 11.5*, 47.9 ± 12.5 material p＜0.01, * p＜0.05 and matched group relatively to bullate rat male and female half and half random packet that influence of rat granuloma as showing, routine disinfection left and right sides armpit, skin incision 0.5cm, to be dipped in 0.1mg ampicillin dry sterilization cotton balls (20mg/) sews up in the posting port respectively, administration 7 days, dosage is the same, put to death animal in the 8th day, open otch, peel off granuloma induced by implantation of cotton pellets, 2h in 60 ℃ of baking ovens, weigh respectively and deduct the former weight of cotton balls, clear granuloma weight, the result shows, heavy dose of Yanminling oral agents and Yanminling nasal spray form performance the obvious suppression effect is arranged rat granuloma is swollen, and low dose of Yanminling oral agents and Yanminling nasal spray to rat granuloma swollen form to show inhibiting trend (table 3) is arranged.Table 3. Yanminling oral agents and Yanminling nasal spray are to rat
Granuloma induced by implantation of cotton pellets influence dosage (g/kg) granuloma dry weight (mg) matched group 44.8 ± 9.8 aspirin 0.1 32.7 ± 11.2* Yanminling oral agents 20 35.4 ± 9.3*
10 36.8 ± 9.5 Yanminling nasal sprays, 20 34.5 ± 10.2*
Compare with matched group 10 37.4 ± 8.4*p＜0.05
Determination of acute toxicity
30 of mices are divided into 2 groups at random, respectively oral Yanminling oral agents 200g/kg and Yanminling nasal spray 24g/kg (disposable oral administration), observed 7 days, 2 groups of mice behaviors as a result are active, and the water inlet feed is normal, hair is smooth, none is only dead, said preparation concentration, and volume has reached maximum, can not survey LD50, real genus is safe in utilization, because people's consumption of Yanminling oral agents is 0.25g/kg every day.
The Yanminling nasal spray is to the nasal membrane irritant test
20 of Cavia porcelluss, body weight 200-250g, male and female half and half are divided into two groups at random: i.e. matched group, Yanminling nasal spray group.Click and enter concentration 0.8g crude drug/ml Yanminling nasal spray 0.5ml in the every 10min of administration group Cavia porcellus nasal cavity; Matched group Cavia porcellus nasal cavity is clicked and entered with the volume aquae destillata, continuity point medicine 5h, in the some medicine process, all animals all occur choking and cough physiological reaction, in the rest point process, all animal activities freely, hair color is smooth, no hogback, perpendicular hairs etc. are symptom unusually.The 24h overall health of patients is no abnormal after observing all animal subject administrations, each group is got 5 sacrifice of animal, anatomical isolation goes out nasal mucosa, and larynx mucosa, tunica mucosa tracheae, bronchial mucosa, lung tissue are done pathology histology procuratorial work, as a result all tested animals above-mentioned organize no abnormal.Each group residue animal was observed 7 days, and diet drinking-water is normal, all animal activities freely, hair color is smooth.
The skin irritation test
4 of male rabbits of white, body weight 2.0-2.5kg, injured skin with the depilation of rabbit back vertebra both sides and not before the experiment, the depilation area is both sides 5 * 10cm.A depilation district makes the normal skin irritant test, is divided into administration district and check plot.Smear Yanminling nasal spray (0.8g/ml) 0.2ml check plot and smear the equivalent tap water in the administration district, cover with the two-layer gauze of one deck oilpaper, the reuse immobilization with adhesive tape, another piece depilation district makes the injured skin stimulation test, abrade in depilation zoning # type with syringe needle before the administration, diameter 2cm is to stab wound epidermis, do not hinder corium, have slight oozing of blood to be degree.The coating method is the same, and opposite side compares.24h after the administration cleans medicinal liquid with wet gauze, writes down the situation that erythema and edema appear in 1,24,48 and, 72 medicine-feeding parts respectively, according to the form below scoring stimulus intensity. skin irritatin extent of reaction standards of grading erythema integration oedema integration do not have clear 2 moderates to severe erythema 3 oedema of erythema 0 no oedema 0 inadequate erythema 1 inadequate visible 1 obvious erythema 2 cutaneous protuberance profiles swell about 1 and model figure enlarge 3 purple erythema and 4 tests of eschar and do not observe the Yanminling nasal spray normal skin and injured skin are had excitant.
To Immune Effects
(1) the mice carbon granule is cleaned up the influence of function
Get 50 of mices and be divided into 5 groups at random, be respectively matched group, scorching Hou spirit oral agents group (20,10g/kg) and Yanminling nasal spray group (20,10g/kg).Every day, mouth was raised administration, and contrast is given with the volume normal saline, successive administration 7 days, and 1h is by tail vein injection carbon ink 0.1ml/kg after the last administration.Then 2,10min cun at mice eye rear vein beard with sharp mouth suction pipe (use in advance heparin moistening) absorption 0.020ml blood, add aquae destillata 4ml, shake up, measure absorbance (0D) value at wavelength 600nm place, calculate phagocytic index K by following formula with 721 type spectrophotometers.
Matched group K value is 0.0109 ± 0.0026 as a result, and Yanminling oral agents high and low dose group is respectively 0.0135 ± 0.0037 (compare with matched group P＜0.05) and 0.0122 ± 0.0028 (compare with matched group P＞0.05); Yanminling nasal spray high and low dose group 0.0130 ± 0.0038 and 0.0126 ± 0.00292, relatively not have significance poor with matched group.
(2) to the influence of delayed hypersensitivity reaction due to the dinitrofluorobenzene (DNFB)
Get 50 of mices and be divided into 5 groups at random, the grouping administration is the same.Sensitization began administration, successive administration 7 days the same day.Preceding 3 days of sensitization, mouse back is stuck with paste depilation with sodium sulfide and is cleaned up, only on depilation portion skin, dripped 1%DNFB acetone soln 100 μ L/ to the 3rd day, 1h reuse 1%DNFB acetone soln 50 μ L attack outside being applied in the mouse right ear portion after the last administration, put to death animal behind the 24h, clip left and right sides ear is got left and right sides ear weighing with diameter 9mm card punch, and the difference of the two weight is the swelling degree.The difference of matched group left and right sides ear weight is 18.7 ± 3.9mg as a result; Yanminling oral agents high and low dose group is respectively 20.5 ± 3.9 and 19.1 ± 3.1, and Yanminling nasal spray high and low dose group is respectively 19.5 ± 4.1 and 16.9 ± 2.4, compares there was no significant difference with matched group.
Three, conclusion
Yanminling oral agents and Yanminling nasal spray have antiinflammatory action, show xylol cause the mice ear inhibitory action, to Ovum Gallus domesticus album cause the inhibitory action of rat paw edema, to the bullate inhibitory action of rat granuloma.
Little to Immune Effects, high dose Yanminling oral agents can promote the mice carbon granule to clean up function, and Yanminling oral agents and Yanminling nasal spray do not have tangible influence to delayed hypersensitivity reaction due to the dinitrofluorobenzene (DNFB).
Yanminling oral agents and Yanminling nasal spray oral toxicity are very little, and skin is not had stimulation.The Yanminling nasal spray to nasal membrane, larynx mucosa, tunica mucosa tracheae, bronchial mucosa, lung tissue is non-stimulated and damaging action.Effect safety.
The present invention will now be further detailed embodiment:
Embodiment one
With 15.5 kilograms of Rhizoma Chuanxiongs, 14.5 kilograms of water distillations of Flos Magnoliae, collect distillate, again with Rhizoma Chuanxiong, 30.5 kilograms of Flos Magnoliae medicinal residues and Mahonia fortunei (Lindl.) Fedde, 29.5 kilograms of Herba Scutellariae Barbataes, 30.5 kilograms of pollen, 29.5 kilograms of Radix Puerariaes, 30.5 kilograms of Polyporus, 29.5 kilograms of the Radixs Astragali, 15.5 kilograms of Fructus Atriplicis Sibiricaees, 8.5 kilograms of Fructus Gleditsia, 8 kilograms of Herba Menthaes, 5.5 kilograms in Radix Glycyrrhizae together, decoct with water twice, each two hours, collecting decoction, filter, filtrate is concentrated in right amount, added 65% alcohol precipitation 24 hours, get supernatant, recovered alcohol, gained medicinal liquid and Rhizoma Chuanxiong, the distillate of Flos Magnoliae merges, and adds an amount of distilled water and simple syrup and promptly obtains 250 kilograms in Yanminling syrup of the present invention (250,000 ml).
Embodiment two
With 14.5 kilograms of Rhizoma Chuanxiongs, 15.5 kilograms of water distillations of Flos Magnoliae, collect distillate, again with Rhizoma Chuanxiong, 29.5 kilograms of Flos Magnoliae medicinal residues and Mahonia fortunei (Lindl.) Fedde, 30.5 kilograms of Herba Scutellariae Barbataes, 29.5 kilograms of pollen, 30.5 kilograms of Radix Puerariaes, 29.5 kilograms of Polyporus, 30.5 kilograms of the Radixs Astragali, 14.5 kilograms of Fructus Atriplicis Sibiricaees, 9.5 kilograms of Fructus Gleditsia, 7 kilograms of Herba Menthaes, 6.5 kilograms in Radix Glycyrrhizae together, decoct with water twice, each two hours, collecting decoction, filter, filtrate is concentrated in right amount, added 65% alcohol precipitation 24 hours, get supernatant, recovered alcohol, gained medicinal liquid and Rhizoma Chuanxiong, the distillate of Flos Magnoliae merges, and adds an amount of distilled water and simple syrup and promptly obtains 250 kilograms in Yanminling syrup of the present invention (250,000 ml).
Embodiment three
With 15 kilograms of Rhizoma Chuanxiongs, 15 kilograms of water distillations of Flos Magnoliae, collect distillate, again with Rhizoma Chuanxiong, 30 kilograms of Flos Magnoliae medicinal residues and Mahonia fortunei (Lindl.) Fedde, 30 kilograms of Herba Scutellariae Barbataes, 30 kilograms of pollen, 30 kilograms of Radix Puerariaes, 30 kilograms of Polyporus, 30 kilograms of the Radixs Astragali, 15 kilograms of Fructus Atriplicis Sibiricaees, 9 kilograms of Fructus Gleditsia, 7.5 kilograms of Herba Menthaes, 6 kilograms in Radix Glycyrrhizae together, decoct with water twice, each two hours, collecting decoction, filter, filtrate is concentrated in right amount, add 65% alcohol precipitation 24 hours, and got supernatant, recovered alcohol, gained medicinal liquid and Rhizoma Chuanxiong, the distillate of Flos Magnoliae merges, and adds an amount of distilled water and simple syrup and promptly obtains 250 kilograms in Yanminling syrup of the present invention (250,000 ml).
Claims (3)
1, a kind of Yanminling syrup and manufacturing process thereof is characterized in that: making 250 kilograms of syrup needs Mahonia fortunei (Lindl.) Fedde 29.5-30.5 kilogram, Herba Scutellariae Barbatae 29.5-30.5 kilogram, pollen 29.5-30.5 kilogram, Radix Puerariae 29.5-30.5 kilogram, Polyporus 29.5-30.5 kilogram, Radix Astragali 29.5-30.5 kilogram, Fructus Atriplicis Sibiricae 14.5-15.5 kilogram, Fructus Gleditsia 8.5-9.5 kilogram, Rhizoma Chuanxiong 14.5-15.5 kilogram, Flos Magnoliae 14.5-15.5 kilogram, 7.8 kilograms of thin He, Radix Glycyrrhizae 5.5-6.5 kilogram.
2, syrup according to claim 1 and manufacturing process thereof is characterized in that: described ten desirable 30 kilograms of big function labor, 30 kilograms of Herba Scutellariae Barbataes, 30 kilograms of pollen, 30 kilograms of Radix Puerariaes, 30 kilograms of Polyporus, 15 kilograms of Rhizoma Chuanxiongs, 15 kilograms of Flos Magnoliae, 7.5 kilograms of Herba Menthaes, 6 kilograms in Radix Glycyrrhizae.
3, a kind of Yanminling syrup and manufacturing process thereof, it is characterized in that: with Rhizoma Chuanxiong, the distillation of Flos Magnoliae water, collect distillate, again with Rhizoma Chuanxiong, Flos Magnoliae medicinal residues with ten big functions, Herba Scutellariae Barbatae, pollen, Radix Puerariae, Polyporus, the Radix Astragali, Fructus Atriplicis Sibiricae, Fructus Gleditsia, Herba Menthae, Radix Glycyrrhizae, decoct with water twice, each two hours, collecting decoction, filter, filtrate is concentrated in right amount, added 65% alcohol precipitation 24 hours, get supernatant, recovered alcohol, the distillate of gained medicinal liquid and Rhizoma Chuanxiong, Flos Magnoliae merges, and adds an amount of distilled water and simple syrup promptly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98122223 CN1228334A (en) | 1998-11-24 | 1998-11-24 | Yanminling syrup and its preparing technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98122223 CN1228334A (en) | 1998-11-24 | 1998-11-24 | Yanminling syrup and its preparing technology |
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| Publication Number | Publication Date |
|---|---|
| CN1228334A true CN1228334A (en) | 1999-09-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 98122223 Pending CN1228334A (en) | 1998-11-24 | 1998-11-24 | Yanminling syrup and its preparing technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100358548C (en) * | 2006-05-22 | 2008-01-02 | 周宣岩 | Chinese medicine rinsing liquid after nasal endoscope operation |
| CN104107256A (en) * | 2014-05-09 | 2014-10-22 | 刘洪池 | Prescription for thoroughly curing rhinitis and nasosinusitis |
-
1998
- 1998-11-24 CN CN 98122223 patent/CN1228334A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100358548C (en) * | 2006-05-22 | 2008-01-02 | 周宣岩 | Chinese medicine rinsing liquid after nasal endoscope operation |
| CN104107256A (en) * | 2014-05-09 | 2014-10-22 | 刘洪池 | Prescription for thoroughly curing rhinitis and nasosinusitis |
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