CN121824747A - 产生双重特异性蛋白的方法 - Google Patents

产生双重特异性蛋白的方法

Info

Publication number
CN121824747A
CN121824747A CN202511879563.7A CN202511879563A CN121824747A CN 121824747 A CN121824747 A CN 121824747A CN 202511879563 A CN202511879563 A CN 202511879563A CN 121824747 A CN121824747 A CN 121824747A
Authority
CN
China
Prior art keywords
protein
antibody
domain
heterodimeric
igg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202511879563.7A
Other languages
English (en)
Chinese (zh)
Inventor
科内利斯·阿德里安·德·克吕夫
弗兰齐斯卡·莫滕森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merus NV
Original Assignee
Merus NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merus NV filed Critical Merus NV
Publication of CN121824747A publication Critical patent/CN121824747A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1271—Micrococcaceae (F); Staphylococcaceae (F), e.g. Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/524—CH2 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202511879563.7A 2022-12-27 2023-12-22 产生双重特异性蛋白的方法 Pending CN121824747A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
NL2033843 2022-12-27
NL2033843 2022-12-27
CN202380092161.5A CN120641444A (zh) 2022-12-27 2023-12-22 产生双重特异性蛋白的方法
PCT/NL2023/050681 WO2024144396A1 (en) 2022-12-27 2023-12-22 Method for generating bispecific proteins

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN202380092161.5A Division CN120641444A (zh) 2022-12-27 2023-12-22 产生双重特异性蛋白的方法

Publications (1)

Publication Number Publication Date
CN121824747A true CN121824747A (zh) 2026-04-10

Family

ID=85158672

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202511879563.7A Pending CN121824747A (zh) 2022-12-27 2023-12-22 产生双重特异性蛋白的方法
CN202380092161.5A Pending CN120641444A (zh) 2022-12-27 2023-12-22 产生双重特异性蛋白的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202380092161.5A Pending CN120641444A (zh) 2022-12-27 2023-12-22 产生双重特异性蛋白的方法

Country Status (9)

Country Link
US (1) US20260209390A1 (cg-RX-API-DMAC7.html)
EP (1) EP4642811A1 (cg-RX-API-DMAC7.html)
JP (1) JP2026500710A (cg-RX-API-DMAC7.html)
KR (1) KR20250129720A (cg-RX-API-DMAC7.html)
CN (2) CN121824747A (cg-RX-API-DMAC7.html)
AU (1) AU2023418349A1 (cg-RX-API-DMAC7.html)
IL (1) IL321671A (cg-RX-API-DMAC7.html)
TW (1) TW202432587A (cg-RX-API-DMAC7.html)
WO (1) WO2024144396A1 (cg-RX-API-DMAC7.html)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4801687A (en) 1986-10-27 1989-01-31 Bioprobe International, Inc. Monoclonal antibody purification process using protein A
US5151504A (en) 1989-11-17 1992-09-29 E. R. Squibb & Sons, Inc. Method for purification of monoclonal antibodies
PT2560993T (pt) 2010-04-20 2024-09-16 Genmab As Proteínas contendo anticorpo heterodimérico fc e métodos para a produção das mesmas
IN2014CN03887A (cg-RX-API-DMAC7.html) * 2011-10-27 2015-10-16 Genmab As
CA3094318A1 (en) 2018-03-30 2019-10-03 Merus N.V. Multivalent antibody
EP3966238A2 (en) 2019-05-09 2022-03-16 Merus N.V. Variant domains for multimerizing proteins and separation thereof
CN116462766A (zh) 2020-05-21 2023-07-21 美勒斯公司 用于生产ig样分子的方法和手段

Also Published As

Publication number Publication date
KR20250129720A (ko) 2025-08-29
JP2026500710A (ja) 2026-01-08
AU2023418349A1 (en) 2025-07-03
WO2024144396A1 (en) 2024-07-04
TW202432587A (zh) 2024-08-16
US20260209390A1 (en) 2026-07-23
IL321671A (en) 2025-08-01
EP4642811A1 (en) 2025-11-05
CN120641444A (zh) 2025-09-12

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