CN1213669C - Functional health-care food with antiradiation, anti free radical and DNA protection - Google Patents
Functional health-care food with antiradiation, anti free radical and DNA protection Download PDFInfo
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- CN1213669C CN1213669C CNB031349129A CN03134912A CN1213669C CN 1213669 C CN1213669 C CN 1213669C CN B031349129 A CNB031349129 A CN B031349129A CN 03134912 A CN03134912 A CN 03134912A CN 1213669 C CN1213669 C CN 1213669C
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Abstract
The present invention relates to a health care food with the functions of radiation resistance, free radical resistance and protection of the functionality of DNA. Anti-oxidation wall-breaking is carried out to ganoderma spore powder at low temperature; crude polyoses is prepared after aqueous extraction or alcohol extraction is carried out to ganoderma fruiting bodies; crude polyoses is extracted according to any method in the application technology from jinsiyi; tea polyphenol extracted from tea raw materials is prepared into capsules or tablets proportionally. The product is suitable for netizens received double radiation, handset people, medical personnel in radiation work chambers and wide people exposed under rays and electromagnetic radiation. The present invention is a plant factor health care product with the functions of protection and repair of damage.
Description
Technical field
The present invention relates to health food, especially a kind of radioresistance, Green Tea Extract also have the functional health-care food of protecting DNA.
Background technology
Ancient times, the mankind only suffer the irradiation of natural radiant point.Modern age, because progress of science and technology, the nuclear atom energy,
60The application of Cor, X ray, radio isotope etc.; The exploitation of new art such as communication apparatus, electrical equipment, electromagnetic field, microwave, new product has been expanded people greatly and has been familiar with the ability of objective world, but also occurred so-called artificial radioactive source's pollution simultaneously.Natural radiation source adds the radiation of man-made source, and both act on human body simultaneously and have increased the weight of the dose of radiation load.Wherein, particularly ionising radiation and electromagnetic radiation endanger very big to the chronic injury of human body to the mankind.As the short-term effect of irradiation damage, consequence at a specified future date, lifelong effect and hereditary effect, also should fully recognize the carcinogenesis of radiation.Known now: penetrability irradiation is that high-energy passes to body, cell, tissue, organ, directly or by free radical destroy the body macromolecular structure indirectly, make it that chain rupture take place, radiation degradation, enzyme is lost activity, cell death, and then make body that a series of biochemistry variation take place, cause organism metabolic disorder, major injury, straight causing death to be died.Especially significantly example is to nucleic acid, claims the damage of inhereditary material or DNA again: bring out the sharp increase of cell micronucleus rate, chromosome aberration and gene mutation, cause a series of irradiation of body and hereditary effect to comprise carcinogenesis.Therefore, the research of radiation injury protection has become contemporary important research project.The protection of radiation is except strengthening strict control measures, and another important protection problem is radiation-resistant medicine of exploitation and functional food, by to often the taking of novel functional food product, reaches and reduces radiation injury or effective for repairing is carried out in radiation injury.The external chemical synthesis that adopts is sought antiradiation drug, even but effectively, price also costliness and side effect is big.Domestic then many exploration from pools of traditional Chinese medicine sought radiation-resistant Chinese medicine, but medicine must have three fens poison.
Summary of the invention
At the deficiency that above-mentioned prior art exists, the present invention proposes a kind of radioresistance, Green Tea Extract and has the functional health-care food of protection DNA, and its process is with the anti-oxidant broken wall of conidia powder low temperature; Ganoderma lucidum fruitbody water carried or alcohol extracting after the thick polysaccharide made; The thick polysaccharide that the spun gold clothing is extracted by any method of declaring in the technology; With the Tea Polyphenols that extracts in the tea raw material, make capsule or sheet type according to a certain ratio, this product is fit to be subjected to netizen, mobile phone crowd, the radiation work chamber medical worker of dual radiation and is exposed to numerous crowds under ray, the electromagnetic radiation, is a kind ofly to have protection and repair the plant factor health products of damage function.
Its assembly of health food provided by the invention is: spun gold clothing extract 10～20%, lucidum spore powder 50～30%, Tea Polyphenols 10～30%, fruiting body extract of Ganoderma Lucidum 30～20%, four kinds of raw materials are mixed, add the food or the medicine glidant mixing of routine again in 10% ratio, get product.
Described spun gold clothing, another name: spun gold tea, deer heart snow tea, blood-snow tea, cladonia fallax lichen, spun gold are risen, Chinese alpine rush, celestial being's hair etc., solely originate in the specific high mountain snowy region of the intense radiation more than 3300 meters of Yunnan height above sea level, anoxic, humidity and temperature, the superfine product that belongs to the pure natural beauty treatment, rare rare, its shape such as coral are rich in multiple components such as atranorine, orange pigment, red star clothing acetoacetic ester.Effect such as have throat-clearing lung-moistening, lowering blood-fat and reducing weight, blood-nourishing blood-generating, calm the nerves is the decline natural dietotherapy treasure of illness such as weak of hypertension, coronary heart disease, hypercoagulable blood, obesity, constipation, pharyngalgia, god, and is without any side effects.Be the special organism of the high stability occurring in nature of heredity, have natural anti-effect of irradiation.Identify through Kunming Inst. of Botany, Chinese Academy of Sciences: belong to natural wild lichens plant, possession clothing [LicHens] type Mycophyta, Usneaceae.
It is the complete stool organism that the present invention makes raw material, gets spun gold clothing raw material, decontamination, drying, breaks into meal, can adopt following three kinds of extracting modes to obtain its extract:
A. get spun gold clothing meal and add the ordinary water lixiviate 2～24 hours of 4～10 times of volumes, filter and collect filtrate, filter residue adds 4～8 times of poach again and carries 2 times, each 1.5～2 hours, filter and remove residue merges three times filtrate, when being concentrated into 1/2 volume, be dried to water content with vacuum freeze drying or spray-drying or 50～60 ℃ of heated dry airs and be no more than 6%, beat powder and preserve standby at normal temperatures; Or
B. get 40～95% ethanolic solutions that spun gold clothing meal adds 4～10 times of PH5～8, extracted in 1.5～3 hours 40～90 ℃ of insulations, extract secondary altogether, filter and remove residue stays filtered fluid, be concentrated into 1/2 volume vacuum freeze drying or spray-drying or 50～60 ℃ of hot dry winds and be dried to water content and be no more than 6%, it is standby to beat powder; Or
C. get spun gold clothing meal supercritical CO
2The substep extraction method obtains some active ingredients respectively, and vacuum freeze drying or be spray dried into water content and be no more than 6% is standby.
Described lucidum spore powder places under-8 to-20 ℃ of cryogenic conditions, and the anti-oxidant broken wall method of physics routinely adds 1.0% ‰～4.0 ‰ antioxidant broken wall and skims the cream off milk, and normal temperature is preserved standby down.
The leaf of described wild tea or artificial cultivation tea, branch are raw material, it is characterized in that: the poach that adds 4～8 times of volumes extracts secondary, each 1.5～2 hours, cooling or natural sedimentation 12～48 hours under 4～6 ℃ of conditions, filter or full alcohol precipitation, filtering and concentrating with vacuum freeze drying or be spray dried into water content and be no more than 6%, promptly gets the crude product that contains Tea Polyphenols during to 1/2 volume, get highly purified Tea Polyphenols elaboration after re-refining, put in storage standby.
Described fruiting body extract of Ganoderma Lucidum is that the employing glossy ganoderma is a raw material, extraction is followed the example of in the water alcohol extracting of broken back, it is characterized in that: added 4～8 times of 60～80% alcohol solution dipping 4～12 hours, reduced-pressure backflow extracted 1.5～2 hours, extracted secondary filter altogether and removed slag merging filtrate, when concentrating filter liquor arrives 1/2 volume of original volume, use vacuum freeze drying instead or be spray dried into water content and be no more than 6%, break into powder, normal temperature is preserved standby.
But described glossy ganoderma raw material ganoderma lucidum fruitbody, ganoderma lucidum mycelium, medicinal lucid ganoderma.
Described functional health-care food is particularly useful for netizen, network application colony, mobile phone to be used the medical worker of crowd, contact radioactive ray and is exposed to numerous crowds under ray, the electromagnetic radiation.
(1) health food that proposes according to the present invention, its toxicity safety testing is finished by food law, the toxicological test safety evaluatio conclusion of this product: this product safe without toxic side effect meets People's Republic of China's food law requirement.
(2) right
60The safeguard function of Cor radiation injury:
A, usefulness
60Cor dosage is the disposable exposure experiment ICR of 7.5Gy mouse, observes 20 days survival rates and peripheral blood WBC number, and the result shows: this product is right
60Cor irradiation mouse institute induced damage resistive has very significantly protective action (P＜0.01).Control group 100% death has more than 50% survival and take this product, and blood picture WBC do not have obvious decline, with control group comparing difference obviously (P＜0.05).
B, usefulness
60Cor dosage is the disposable exposure experiment ICR of 7.5Gy mouse, to bring out mouse bone marrow cells micronucleus and statistical analysis, evaluation this product to DNA protective effect and effect.Result of the test shows: the moulding control group brings out microkernel incidence up to 12.50 ± 3.89%, and uses the protection group of this product to bring out microkernel incidence a little more than normal value (3 ‰) upper limit, is respectively 4.00 ± 0.81; 5.20 ± 1.68; 4.20 ± 1.47.But far below the moulding control group, difference highly significant (P＜0.001) is protected efficiently to reach 64.39%.This results suggest: this product has very strong Antiradiation injury function.Body tissue, organ, inhereditary material/nucleus had the excellent protection effect.
(3) protective effect that chemical substance is damaged
A. endoxan (Cyclophosphamidum) is the mutagenesis of generally acknowledging both at home and abroad---the distortion material has the obvious impairment effect to body bone marrow cell nuclear/chromosome.The inventor irritates stomach for the animal that is put to the test with this product as protective agent, and result of the test proves: the micronucleus recall rate that the animal that is put to the test of use this product is brought out is starkly lower than unshielded positive controls (P＜0.01).Chromosomal observation is also found: use the protection group chromosome aberration rate (meaning that part, disappearance, ring, dicentromere, aneuploid etc. appear in chromosome) of this product obviously low than positive controls; difference highly significant (P＜0.01,0.05); thereby explanation this product is to inhereditary material/chromosome; have the excellent protection function, equally also have prevention cell mutation protective effect on cancer risk.
Health product of the present invention is reasonable because of the raw materials used uniqueness of filling a prescription, craft science, thereby has tangible multifunctional anti-radiation effect, and is efficient up to 64.38% through animal experiment.Contained multiple material provides as nutrient raw material or participates in the body biochemistry metabolism, promotes the wound repair process with bioactivator, improves the immunity of organism regulating power, has improved tolerance and the adaptability of body to harmful substance.
Description of drawings:
Fig. 1 is a spun gold clothing extraction process flow chart among the present invention.Fig. 2 is lucidum spore powder processing process figure.Fig. 3 is a Tea Polyphenols extraction process flow chart.Fig. 4 is a glossy ganoderma extraction process flow chart.
The specific embodiment: be illustrated in conjunction with the accompanying drawings:
Spun gold clothing decontamination, be dried and crushed into meal, can be made into the tea in bag drink.As processing raw material, the extraction process flow process of spun gold clothing total extract can adopt following any, as Fig. 1:
A, get the ordinary water lixiviate 24 hours that spun gold clothing meal adds 4～10 times of volumes, after getting filtrate, filter residue adds 2～8 times of poach again and carries secondary, each 1.5～2 hours, filter and remove residue, merge three filtrate, when being concentrated to 1/2 volume, vacuum freeze drying or spray-drying or 50～60 ℃ of dry powder of beating of heated dry air.Gained dry powder kept dry at normal temperatures is standby;
B, get spun gold clothing meal, add 4～10 times, secondary is extracted in 40～90 ℃ of insulations of 40～95% ethanolic solutions of PH5～8, each 1.5～3 hours, filter waste, concentrate and cause 1/2 volume, vacuum freeze drying or spray-drying or 50～60 ℃ of hot dry wind dryings are beaten powder, and kept dry is standby at normal temperatures;
C, get the spun gold clothing and place supercritical CO
2In the fractional extraction groove, extract several active components, get several one-components with vacuum freeze drying or spray drying treatment respectively, merging is spun gold clothing total extract powder or the semi-finished product dry powder that a, b processed is evenly promptly got spun gold clothing total extract to equal portions.
1. cloud lucidum spore powder processing process:
Get the cloud lucidum spore powder, under-8 to-20 ℃ of cryogenic conditions, adding 1 ‰～4 ‰ antioxidant broken walls dry the dry powder that skims the cream off milk be semi-finished product, preserves standby under the air drying condition.
2. wild ancient tea polyphenols processing extraction technique:
The selected tealeaves of getting adds 4～8 times of volume water again and boils and boil the extraction secondary, each 1.5～2 hours, filters waste.Cooling merges secondary raffinate natural sedimentation 48 hours under 4～6 ℃ of conditions, filter, or full alcohol precipitation filters, and concentrates vacuum freeze drying or spray-drying and promptly gets the Tea Polyphenols crude product that contains 30-40%.Get highly purified Tea Polyphenols elaboration after re-refining, promptly the semi-finished product warehouse-in is standby.
3. glossy ganoderma extraction process:
4～8 times of 60～80% alcohol solution dipping of the broken adding of selected high-quality cloud glossy ganoderma 4～12 hours, reduced-pressure backflow extracted 1.5～2 hours, extracting secondary filter altogether removes slag, merging filtrate concentrates appropriate vacuum freeze drying/spray-drying/heated-air drying and gets the glossy ganoderma total extract, beat powder and promptly get semi-finished product, normal temperature is preserved standby.
Above gained semi-finished product, its water content must not surpass 6%, 5 years shelf-lifves.
4. will process the semi-finished product of gained, by 10～20: 50～30: 10～30: 30～20 part by weight mix, and encapsulation promptly can be used for producing, such as the health food of formulations such as tablet, pill, capsule.
Example I:
1. get spun gold clothing meal 10.0kg, add 100kg ordinary water lixiviate 24 hours.After getting filtrate, filter residue adds the 80kg ordinary water again and boils and carry secondary, each 1.5～2 hours.Filter waste, merge three times and extract filtered fluid, be concentrated to 1/2 amount of original volume, use vacuum freeze drying instead and get the about 3.01kg of dry powder; Standby.
2. get the cloud lucidum spore powder and add 1 ‰ Tea Polyphenols under-10 ℃ of conditions, broken wall skims the cream off milk, and processes to such an extent that the 3.01kg conidia powder is standby.
3. get wild tea 10.0kg, adding 80.0kg ordinary water boils and boils the extraction secondary, each 2 hours.Filter waste.Merge secondary raffinate.Natural sedimentation is 48 hours in 4～6 ℃ of freezers, filters, and uses spray-drying when being concentrated to 1/2 volume.Re-refine about elaboration Tea Polyphenols 1.5kg.
4. get glossy ganoderma 10.0kg, add 60% ethanol 80.0kg and soaked 12 hours, reduced-pressure backflow extracted 2 hours, extracted secondary altogether.Filter waste, merging filtrate is concentrated to 1/2 amount of original volume, and changing spray-drying, to get the about 1.0kg of dry powder semi-finished product standby.
5. get spun gold clothing semi-finished product 100 grams by formula rate requirement (1); (2) get lucidum spore powder elite 500 grams; (3) wild ancient tea polyphenols 100 grams; (4) get cloud Ganodenna Lucidum P.E 300 grams.Four kinds of raw material semi-finished product are mixed, promptly get 1000 gram materials.The glidant mixings that add 100 grams are again filled with No. zero or No. 1 Capsules and to be bottled or plastic-aluminum bubble-cap pressing plate gets product.
Example II:
1, get spun gold clothing meal 10kg, 40% the ethanolic solution that adds 100kg, PH5 extracted 3 hours 40 ℃ of insulations, extracted secondary.Filter waste, when merging filtrate is concentrated to 1/2 volume, uses spray-drying instead and beat powder and get semi-finished product 3kg, it is 6% standby that water content is no more than.
2, get lucidum spore powder and under-8 ℃ of conditions, add 2.5 ‰ n-propyl gallate broken wall and skim the cream off milk with the biophysics method, process semi-finished product 3kg, it is 6% standby that water content is no more than.
3, get tea 10kg, adding 50kg ordinary water boils and boils the extraction secondary, each 1.5 hours, filters waste.Merge second extract, cooling, natural sedimentation pressure filtration in 24 hours, concentrated filtrate are used vacuum freeze drying instead during to 1/2 volume, pressure filtration when re-refining, when concentrated filtrate causes 1/2 volume, use vacuum freeze drying instead, re-refine once, promptly get about elaboration Tea Polyphenols 1.5kg, it is 6% standby that water content is no more than.
4, get glossy ganoderma 10kg, the ethanol 60kg of broken back adding 70% soaked 12 hours, subtracted and refluxed 1.5 hours, altogether the refluxing extraction secondary.Filter and remove residue, merging filtrate during concentrated filtrate to 1/2 volume, is used vacuum freeze drying instead, and water content is no more than 6%, is total to such an extent that semi-finished product 1kg is standby.
5, get (1) spun gold clothing semi-finished product 120 grams in the formulation weight ratio; (2) lucidum spore powder elite 480 grams; (3) Tea Polyphenols 150 grams; (4) Ganodenna Lucidum P.E 250 grams.Four kinds of raw meal are mixed, promptly get 1000 gram factory supplies.Add 100 gram glidant mixings again, fill with No. zero or No. 1 Capsules and bottle or plastic-aluminum bubble-cap pressing plate gets product.
EXAMPLE III:
1, get spun gold clothing meal 10kg, 95% the ethanolic solution insulation that adds the pH8 of 100kg is extracted secondaries for 90 ℃, and each 1.5 hours, filter waste, when being concentrated into 1/2 volume, use 55 ℃ of hot dry wind dryings instead, water content is no more than 6%, and it is standby to beat powder.
2, get lucidum spore powder under-20 ℃ of cryogenic conditions, add 3 ‰ antioxidant vitamin E with the biophysics method, broken wall skims the cream off milk, process the 3kg conidia powder, it is 6% standby that water content is no more than.
3, get qualified tea 10kg, add water 60kg, boil the extraction secondary, each 2 hours.Filter waste.Merge secondary raffinate, cooling.Add 99% ethanol 100kg precipitation, filter, reclaim ethanol, when being concentrated into 1/2 volume, use the hot dry wind drying instead, re-refine and once promptly get elaboration Tea Polyphenols 1.2kg left and right sides semi-finished product, it is 6% standby that water content is no more than.
4, get glossy ganoderma 10kg, add 80% ethanol liquid 50kg and soaked 12 hours, reduced-pressure backflow extracted 2 hours, extracted altogether 2 times, filtered waste, and merging filtrate is used heated-air drying instead when being concentrated into 1/2 volume, and it is 6% standby that water content does not surpass.
5, get (1) spun gold clothing semi-finished product 150 grams in the formulation weight ratio; (2) get lucidum spore powder semi-finished product 450 grams; (3) get semi-finished product Tea Polyphenols 250 grams; (4) get fruiting body extract of Ganoderma Lucidum semi-finished product 150 grams.Four kinds of semi-finished product are evenly mixed, promptly get 1000 gram factory supplies.Add 100 gram glidant mixings again, fill with No. zero or No. 1 Capsules and bottle or plastic-aluminum bubble-cap pressing plate gets product.
EXAMPLE IV:
1, gets spun gold clothing meal 10kg, place supercritical CO
2In the extraction tank, use different solvents, under super critical condition, the fractional extraction active component gets some kinds of one-components respectively, and vacuum freeze drying gets the single-activity composition respectively, the mixture of these single components is the about 1kg of spun gold clothing total extract semi-finished product, and it is 6% standby that water content is no more than.
2, get lucidum spore powder under-15 ℃ of cryogenic conditions, add 4 ‰ vitamin C broken wall with the biophysics method and skim the cream off milk, process to such an extent that semi-finished product 3kg conidia powder, water content are no more than 6% standby;
3, get qualified tea 10kg, add water 80kg and boil the extraction secondary, each 2 hours, to filter, cooling adds 100kg absolute ethyl alcohol sedimentation and filtration, and merging filtrate reclaims ethanol, and when filtrate was concentrated into 1/2 volume, spray-drying got semi-finished product, and water content is standby less than 6%.
4, soaked 4 hours with 75% ethanol 70kg after the glossy ganoderma fragmentation, reduced-pressure backflow extracted 1.5 hours, carry altogether 2 times, and filter and remove residue, merging filtrate is used spray-drying instead and is made the semi-finished product for standby of grain when being concentrated into 1/2 volume, and water content is less than 6%.
5, get (1) spun gold clothing means of supercritical extraction semi-finished product 200 grams by formula rate; (2) get lucidum spore powder semi-finished product 300 grams; (3) get Tea Polyphenols semi-finished product 300 grams; (4) get Ganodenna Lucidum P.E semi-finished product 200 grams, above four kinds of semi-finished product raw materials are evenly mixed promptly getting 1000 gram factory supplies; Add 100 gram glidant mixings again, fill with No. zero or No. 1 Capsules and bottle or plastic-aluminum bubble-cap pressing plate gets product.
Claims (6)
1, a kind of functional health-care food, it is characterized in that assembly is: spun gold clothing extract 10～20%, ganoderma spove powder 50～30%, Tea Polyphenols 10～30%, fruiting body extract of Ganoderma Lucidum 30～20%, four kinds of raw materials are mixed, add the food or the medicine glidant mixing of routine again in 10% ratio, get product.
2, functional health-care food as claimed in claim 1 is characterized in that: described spun gold clothing extract is to get spun gold clothing raw material, decontamination, drying, breaks into meal, adopts that any extracting mode makes in following three kinds of methods:
A. get spun gold clothing meal and add the ordinary water lixiviate 2～24 hours of 4～10 times of volumes, filter and collect filtrate, filter residue adds 4～8 times of poach again and carries 2 times, each 1.5～2 hours, filter and remove residue merges three times filtrate, when being concentrated into 1/2 volume, be dried to water content with vacuum freeze drying or spray-drying or 50～60 ℃ of heated dry airs and be no more than 6%, beat powder and preserve standby at normal temperatures; Or
B. get 40～95% ethanolic solutions that spun gold clothing meal adds 4～10 times of PH5～8, extracted in 1.5～3 hours 40～90 ℃ of insulations, extract secondary altogether, filter and remove residue stays filtered fluid, be concentrated into 1/2 volume vacuum freeze drying or spray-drying or 50～60 ℃ of hot dry winds and be dried to water content and be no more than 6%, it is standby to beat powder; Or
C. get spun gold clothing meal supercritical CO
2The substep extraction method obtains some active ingredients respectively, and vacuum freeze drying or be spray dried into water content and be no more than 6% is standby.
3, functional health-care food according to claim 1, it is characterized in that: described ganoderma spove powder is to get lucidum spore powder to place under-8 ℃ to-20 ℃ cryogenic conditions, the anti-oxidant broken wall method of physics routinely adds 1.0 ‰～4.0 ‰ antioxidant broken walls and skims the cream off milk, and normal temperature is preserved standby down.
4, functional health-care food according to claim 1, it is characterized in that: wherein said Tea Polyphenols is that leaf, the branch of getting wild tea or artificial cultivation tea are raw material, the poach that adds 4～8 times of volumes extracts secondary, each 1.5～2 hours, cooling or under 4～6 ℃ of conditions natural sedimentation 12-48 hour, filter or full alcohol precipitation, filtering and concentrating during to 1/2 volume with vacuum freeze drying or be spray dried into water content and be no more than 6%, promptly get the crude product that contains Tea Polyphenols, get highly purified Tea Polyphenols elaboration after re-refining, put in storage standby.
5, functional health-care food according to claim 1, it is characterized in that: described fruiting body extract of Ganoderma Lucidum is that the employing glossy ganoderma is a raw material, extraction is followed the example of in the water alcohol extracting of broken back, adds 4～8 times of 60～80% alcohol solution dipping 4～12 hours, reduced-pressure backflow extraction 1.5～2 hours, extracting secondary filter altogether removes slag, merging filtrate, concentrating filter liquor be during to 1/2 volume of original volume, uses vacuum freeze drying instead or be spray dried into water content to be no more than 6%, break into powder, normal temperature is preserved standby.
6, functional health-care food according to claim 5 is characterized in that: but glossy ganoderma raw material ganoderma lucidum fruitbody, ganoderma lucidum mycelium, medicinal lucid ganoderma.
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| CN109924474A (en) * | 2019-04-04 | 2019-06-25 | 上海禾向健康科技发展有限公司 | A kind of composition of radiation protection, ganoderma lucidum tea polyphenols health food and preparation method thereof |
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