CN1212333C - Soybean segregative scleroprotein clean production method - Google Patents
Soybean segregative scleroprotein clean production method Download PDFInfo
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- CN1212333C CN1212333C CN 02112016 CN02112016A CN1212333C CN 1212333 C CN1212333 C CN 1212333C CN 02112016 CN02112016 CN 02112016 CN 02112016 A CN02112016 A CN 02112016A CN 1212333 C CN1212333 C CN 1212333C
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Abstract
The present invention relates to a clean production method for separating soybean proteins, which is characterized in that wastes produced in the whole production process for separating the soy proteins are changed into resources. After being processed in an integrated mode, waste water of soybeans is cyclically used to obtain soybean oligosaccharide, whey proteins and isoflavone; soybean skins and soybean residues are used for producing dietary fiber; bean germs are used for producing soyasaponin, germ oil and plumule proteins; oil residues are used for producing phospholipid. The method thoroughly solves the problem that the waste water and the waste residues, which are generated by separating the soybean proteins, pollutes the environment; except for the series of isolation proteins of main products, raw materials of functional food with high subsidiary value, such as the soybeans, the dietary fiber, soybean whey proteins, etc., are also produced.
Description
Technical field: the present invention relates to soybean protein clearer production technology field, belong to specifically and the comprehensive utilization of soybean produced the functional substance of soybean isoflavones, saponin, food fibre, the contour secondary valence value of oligose is especially free from environmental pollution, the production method of the soybean protein of conserve water resource.
Background technology: about soybean protein production technology industry, belong to hi-tech industry, development is very fast in China, existing at present more than 30 families, but 1 ton of protein isolate of every production can produce more than 20 ton of COD surpasses 20000mg/l, and BOD surpasses organic wastewater with high concentration and the 5-6 ton waste residue of 10000mg/l.The general treatment process of present protein isolate production technique is all direct discharging of waste water or friendship to be advanced the waste water processing station processing, and waste residue (bean dregs, skin of beancurd, beans embryo and the dregs of fat etc.) is sold feed producer.Serious environmental pollution, the wasting of resources have been caused.Cause the increase of production cost, in intense market competition for inferior position, according to our investigation, in the isolated in China albumen factory, ordinary production be less than half, if take process for cleanly preparing, can not only control pollution, conserve water resource, and a bit not loss of the valuable components of soybean, product rises in value more than ten times.
Summary of the invention: the objective of the invention is to fully utilize the various nutritive ingredients in the soybean, realize the zero release of waste water, waste residue in the whole process of production, the clean preparation method of the soybean protein isolate of environmentally safe.
The technical scheme of taking for the purpose of finishing foregoing invention is as follows: a kind of method of cleaner production of soybean protein isolate, low temperature after removing the peel, go embryo behind the soybean intensive sorting is leached, obtain crude oil of soybean and low temperature soy meal, low temperature soy meal is molten and sour heavy through alkali, and centrifugation obtains bean dregs, albumen mud and waste water; With direct spraying drying behind the albumen mud accent pH or through biological enzyme mainly is that protease treatment obtains water-soluble protein isolate, soybean polypeptide or plant protein hydrolysate; Waste water is handled directly multiple-effect vacuum concentration of back, obtain concentrated solution and water of condensation, concentrated solution is separated obtaining soybean oligosaccharide, whey-protein and soybean isoflavones again; Described skin of beancurd and bean dregs are carried out deep processing production food fibre; Described beans embryo is extracted Soyasaponin, germ oil and soybean protein; Described crude oil of soybean is carried out concise soybean oil and the soybean phospholipid of obtaining, it is characterized in that, obtain that soybean oligosaccharide adopts be by the concentrated solution that obtains after the wastewater treatment carry out Concentrated and crystallized in vacuum, centrifugal, separate, the method for oven dry; Describedly get back in the molten water of dregs of beans alkali, reach water circulation use through spissated water of condensation.
The clean preparation method of soybean protein isolate of the present invention has thoroughly solved the waste water in the protein isolate production engineering, the pollution problem of waste residue.Except that major product soybean oil, protein isolate series, soybean polypeptide, also obtain functional food ingredients such as soybean oligosaccharide, Soyasaponin, soybean isoflavones, soybean whey protein, soybean phospholipid, soybean dietary fibre.Greatly reduce production cost, obtained huge economic benefit and social benefit.Three unifications of environmental benefit, economic benefit and social benefit have been realized.
Description of drawings:
Fig. 1 is a process flow sheet of the present invention.
Embodiment: as shown in Figure 1: the first step with soybean intensive sorting after peeling, go that low temperature leaches behind the embryo, obtain mixing oil and low temperature soy meal, low temperature soy meal is after " alkali is molten and sour heavy " operation, obtain bean dregs, albumen mud and soybean waste water, again with soybean protein mud through spraying drying protein isolate behind the adjust pH, or add biotechnological formulation process for producing soybean polypeptide, water-soluble protein product such as proteolytic enzyme; Secondly soybean waste water is handled directly multiple-effect vacuum concentration of back, obtain concentrated solution and water of condensation, water of condensation is got back to the molten water of dregs of beans alkali, reach water circulation use, again concentrated solution is stayed the membrane sepn whey-protein of scope at 1500-3000 with macromolecule flocculation agent flocculation separation whey-protein or with the molecule loading gage, vacuum concentration liquid Crystallization Separation soybean oligosaccharide then, with organic solvent extraction isoflavones such as ethanol, obtain the soybean oligosaccharide that higher pair is worth, product such as whey-protein and soybean isoflavones, or the direct spraying drying of concentrated solution obtained containing soybean oligosaccharide, the mixing functions raw material of whey-protein and isoflavones, again skin of beancurd and bean dregs are carried out deep processing production food fibre, beans are paid for extracted germ oil, Soyasaponin and soybean protein; Last soybean mixes the right side and isolates solvent and crude oil, again crude oil aquation, refining is obtained soybean and phosphatide.
Embodiment one:
Low temperature leaches: on market, purchase 1 kilogram of northeast soybean, selected, oven dry, broken decortication, take off embryo, skin of beancurd 90 grams, beans embryo 23 grams, bean cotyledon is crushed to more than 60 orders, adds No. 6 solvent refluxings and leaches, and obtains the wet dregs of rice and mixing oil, mixing oil vacuum removal solvent, obtain crude oil 162 gram and solvents (recovery), the wet dregs of rice get low temperature soy meal 712 grams through the vacuum precipitation, protein content 53.8%, nitrogen dissolubility index (NSI) 87.5%;
Alkali extraction and acid precipitation technology is extracted albumen: with the hot water of 10 times 50-55 ℃ of above-mentioned low temperature soy meal adding, transfer pH7.0-7.5, stir extraction 15 minutes, 5000 change above centrifugation, wash bean dregs 2-3 time.Obtain water content 81.4% bean dregs 1200 gram and soya-bean milk liquid, transfer soya-bean milk liquid pH4.4-4.5 with hydrochloric acid, centrifugation obtains containing 8.5 liters of the whey wastewaters and soybean milk curd albumen mud of solid substance 1.25%;
Produce protein product: the albumen mud of alkali extraction and acid precipitation technology is added water become the 10-15% soy protein suspension, attemperation adds 10% papoid liquid in 40 ℃, regulate pH to 8.0, constantly the NaOH with 0.05N keeps pH value 8.0, according to NaOH volume control hydrolysis degree, after reaching certain degree of hydrolysis, the hydrochloric acid solution adjust pH about 7.0 that adds 0.05N, be warming up to 85-90 ℃ simultaneously, reaction times 15-20 minute, make enzymatic inactivation, spraying drying gets 345 gram protein contents, 89.3% albumen.If the control hydrolysis degree is at the water-soluble and dispersed good soybean injection of 5% left and right sides Ke De protein isolate, if the control hydrolysis degree is about 25, can produce soybean polypeptide, if the control hydrolysis degree about 90%, can be produced plant protein hydrolysate, if not hydrolysis, after transferring pH7.0, intensification 85-90 ℃ sterilization, take off raw meat, direct spraying drying, common protein isolate;
Water reuse: the whey wastewater of alkali extraction and acid precipitation is concentrated 10-15 doubly at vacuum tightness 0.8Mpa, obtain (temperature 50-60 ℃ of 7.8 liters of water of condensation, COD＜150mg/l, can be used for the molten water of next batch alkali) and concentrated solution, solid concentration 15-20%, add food-grade polypropylene acid sodium 10-20ppm in concentrated solution, dissolved air flotation goes out whey-protein, and vacuum drying gets whey-protein 50 grams of protein content 91.5%; Concentrated solution continues Concentrated and crystallized in vacuum, centrifugation, and oven dry gets soybean oligosaccharide 66 grams, oligosaccharide contg 85.8%; Clear liquid vacuum-drying, obtain 10% soybean isoflavones product, wherein soybean isoflavones contains 10.2%, whey-protein 31.7%, soybean oligosaccharide content 43.5%, if will obtain the higher soybean isoflavones of concentration, 10% soybean isoflavones product is added 75% alcohol extraction, can obtain the product of 30% above isoflavone content;
Produce food fibre: skin of beancurd and alkali extraction and acid precipitation technology that low temperature leaches are extracted. bean dregs add alkali cellulose enzymic hydrolysis 30 minutes, heat up 75 ℃, add 0.5% hydrogen peroxide decolouring 30 minutes, filter, dry to moisture content about 20%, small-sized Luo bar extrusion machine with French import is handled at 175-180 ℃ of extruding puffing, gets protein content 20.3%, food fibre 403 grams of dietary fiber content 71.7%.
The utilization of plumule: the soybean germ that low temperature leaches is pulverized, add No. 6 solvent refluxing extractions, leach final vacuum recovery solvent and obtain the germ oil and the plumule dregs of rice, the plumule dregs of rice add 75% aqueous ethanolic solution, in temperature is to carry out continuous 3 lixiviates 3 hours under 75 ℃, separate, vacuum reclaims ethanol, obtain protein content 71.5% germ protein and Soyasaponin mixture, in the Soyasaponin mixture, add 1: 1 n-butanol aqueous solution of 10ml, separate solvent recovered under vacuum, drying obtains Soyasaponin 1.5 grams, wherein Soyasaponin content 53.1%, soybean isoflavones 11.2% and soybean oligosaccharide 2.8 grams;
The dregs of fat are produced phosphatide: with the crude oil hydration degum that low temperature leaches, separate obtaining oil foot and oil, the dehydration of oil foot vacuum concentration, decolouring obtain concentrated soybean phospholipid, and wherein acetone insoluble matter 63.2%; Oil dehydration, refining obtain soybean secondary oil, and refining can obtain soybean salad oil again.
Embodiment two: with embodiment one, just the separation of the whey-protein in the water reuse technology is adopted and is stayed the polysulfone membrane sepg whey albumen of scope at 1500-3000 with the molecule loading gage.
Embodiment three:
1 step with reference to the accompanying drawings, handle in day and to handle 1 ton of soybean on 10 tons the soy protein production line:
Low temperature leaches: embryo is peeled, is taken off in soybean intensive sorting, oven dry, fragmentation, get 102 kilograms of skin of beancurd, 26 kilograms of beans embryos, bean cotyledon add No. 6 solvent extraction after softening, rolling embryo, obtain the wet dregs of rice and mixing oil, mixing oil vacuum removal solvent, obtain 154 kilograms of crude oils and solvent (reclaiming circulation is used for leaching), the wet dregs of rice get 715 kilograms of low temperature soy meals through 140 ℃ of high temperature flash distillation precipitations and 70 ℃ of vacuum secondary precipitations, protein content 52.5%, nitrogen dissolubility index (NSI) 86.5%;
Alkali extraction and acid precipitation technology is extracted albumen: the hot water that the low temperature soy meal of low temperature leaching is added 10 times 50-55 ℃, transfer pH7.0-7.5, stir extraction 15 minutes, 4000 change above centrifugation, and bean dregs heavily come together once, obtain 1200 kilograms in water content 80.2% bean dregs and soya-bean milk liquid, soya-bean milk liquid is transferred pH4.4-4.5 with hydrochloric acid, stir, centrifugation obtains containing 8 tons of the whey wastewaters and the soybean milk curd albumen mud of solid substance 1.2%;
Produce protein product: the albumen mud that low temperature leaches is transferred pH8.0, add papain hydrolysis, transfer pH with NaOH, keep pH 8.0, (if the control hydrolysis degree about 25, can be produced soybean polypeptide, degree of hydrolysis is about 90% producing injection albumen about 5% for the control hydrolysis degree, can produce plant protein hydrolysate), transfer pH7.0, heat up and go out 140 ± 5 ℃, the flash distillation enzyme and sterilizing that goes out, last spraying drying gets 2 kilograms of water-soluble and dispersed good soybean injection protein 31s;
Water reuse: the whey wastewater five of alkali extraction and acid precipitation is imitated falling liquid film concentrate, first imitates 115-120 ℃ of temperature, 12-15 ℃ of every effect temperature difference is concentrated to solid substance 15-20%, spraying drying, obtain whey protein 40%, soybean oligosaccharide 52%, soybean isoflavones 0.8% functional mixture obtains 7.2 tons (temperature 50-60 ℃ of water of condensation simultaneously, COD＜150mg/l can be used for the molten water of next batch alkali or it is used);
Produce food fibre: skin of beancurd and alkali extraction and acid precipitation that low temperature is leached. bean dregs add alkali cellulose enzymic hydrolysis 30 minutes, heat up 75 ℃, add 0.5% hydrogen peroxide decolouring 30 minutes, heat up 140 ± 5 ℃ again, the flash distillation enzyme and sterilizing that goes out takes off raw meat, last spraying drying gets protein content 18.5%, 398 kilograms of the food fibres of dietary fiber content 70.6%;
The utilization of plumule: the soybean germ that low temperature is leached adds 31 kilograms of No. 6 solvents, solvent ratio 1: 1.2, extraction temperature is about 53 ℃, leach the final vacuum solvent of turning one's head and obtain the germ oil and the plumule dregs of rice, the plumule dregs of rice add 75% aqueous ethanolic solution, are to carry out continuous 3 lixiviates 3 hours under 75 ℃ in temperature, separate, vacuum is returned glucoside 2.9%, soybean isoflavones 1.3%.
The utilization of the dregs of fat: with the crude oil hydration degum that low temperature leaches, separate obtaining oil foot and oil, the dehydration of oil foot vacuum concentration, decolouring obtain concentrated soybean phospholipid, and wherein acetone insoluble matter 63.2%; Oil dehydration, refining are obtained soybean secondary oil, and refining can obtain soybean salad oil again.
Claims (1)
1. the method for the cleaner production of a soybean protein isolate leaches low temperature after removing the peel, go embryo behind the soybean intensive sorting, obtains crude oil of soybean and low temperature soy meal, and low temperature soy meal is molten and sour heavy through alkali, and centrifugation obtains bean dregs, albumen mud and waste water; With direct spraying drying behind the albumen mud accent pH or through biological enzyme mainly is that protease treatment obtains water-soluble protein isolate, soybean polypeptide or plant protein hydrolysate; Waste water is handled directly multiple-effect vacuum concentration of back, obtain concentrated solution and water of condensation, concentrated solution is separated obtaining soybean oligosaccharide, whey-protein and soybean isoflavones again; Described skin of beancurd and bean dregs are carried out deep processing production food fibre; Described beans embryo is extracted Soyasaponin, germ oil and soybean protein; Described crude oil of soybean is carried out concise soybean oil and the soybean phospholipid of obtaining, it is characterized in that, obtain that soybean oligosaccharide adopts be by the concentrated solution that obtains after the wastewater treatment carry out Concentrated and crystallized in vacuum, centrifugal, separate, the method for oven dry; Describedly get back in the molten water of dregs of beans alkali, reach water circulation use through spissated water of condensation.
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| CN 02112016 CN1212333C (en) | 2002-06-10 | 2002-06-10 | Soybean segregative scleroprotein clean production method |
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| CN 02112016 CN1212333C (en) | 2002-06-10 | 2002-06-10 | Soybean segregative scleroprotein clean production method |
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| CN1212333C true CN1212333C (en) | 2005-07-27 |
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Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288437B (en) * | 2007-04-20 | 2012-08-08 | 四川久大制盐有限责任公司 | Method for producing soy protein peptides and dietary fiber from defated soybean pulp |
| CN101455398B (en) * | 2008-12-31 | 2012-05-23 | 深圳职业技术学院 | Soya-dregs water soluble diet fiber preparation method using nano filtration and spray-drying method |
| CN101627814B (en) * | 2009-08-06 | 2012-05-23 | 山东冠华蛋白有限公司 | Soybean milk protein compound and preparation method thereof |
| CN102850428B (en) * | 2012-04-05 | 2014-08-27 | 张祥成 | Method and device for realizing soybean protein separation by two mediums-mixed leaching |
| CN102847615B (en) * | 2012-08-30 | 2013-09-25 | 山东理工大学 | Centrifugal embryo-skin separating machine with adjustable separation rate |
| CN103766573A (en) * | 2013-12-27 | 2014-05-07 | 山东禹王生态食业有限公司 | Production method for soy isolate protein applied to solid beverage |
| CN103766577B (en) * | 2014-02-28 | 2015-05-13 | 北京金地三福膨化机制造(大厂)有限公司 | Method for preparing active proteins from soybean hulls and application of active proteins |
| CN104115990B (en) * | 2014-06-17 | 2017-09-08 | 哈尔滨旭康农业高科技有限公司 | A kind of preparation method of high-quality water soluble soybean protein mixed powder and products thereof |
| CN104530203B (en) * | 2014-12-04 | 2019-05-10 | 大兴安岭林格贝寒带生物科技股份有限公司 | The method of high purity protein is obtained from wild nettle |
| CN104889141B (en) * | 2015-05-28 | 2017-02-22 | 山东理工大学 | Comprehensive utilization method for soybean dregs |
| CN107660692A (en) * | 2016-07-27 | 2018-02-06 | 高士敏 | A kind of production method of instant bean curd powder |
| CN106820215B (en) * | 2016-12-26 | 2019-01-22 | 重庆保卫食品有限公司 | Removing method for beans plumule |
| CN108191950A (en) * | 2017-12-30 | 2018-06-22 | 临邑禹王植物蛋白有限公司 | A kind of method that soybean protein is recycled from soya whey wastewater |
| CN110540532B (en) * | 2019-06-17 | 2021-06-01 | 山东禹王生态食业有限公司 | Method for extracting soybean isoflavone from soybean milk water first-stage scum |
| CN111944010B (en) * | 2020-08-20 | 2023-05-09 | 运城学院 | Extraction equipment and method for soybean protein, isoflavone and oligosaccharide in bean product wastewater |
| CN112724194A (en) * | 2021-01-18 | 2021-04-30 | 荣海生物科技有限公司 | Method for extracting peptide from protein hydrolysis by-product |
| CN114478689B (en) * | 2022-03-11 | 2022-11-15 | 岭南师范学院 | Isoflavone peptide compound, preparation method and application thereof, and Hakka mother wine containing isoflavone peptide compound |
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