CN120584195A - 源自核糖体rna的逆转录的抑制 - Google Patents

源自核糖体rna的逆转录的抑制

Info

Publication number
CN120584195A
CN120584195A CN202480008982.0A CN202480008982A CN120584195A CN 120584195 A CN120584195 A CN 120584195A CN 202480008982 A CN202480008982 A CN 202480008982A CN 120584195 A CN120584195 A CN 120584195A
Authority
CN
China
Prior art keywords
reverse transcription
group
rna
polymer
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202480008982.0A
Other languages
English (en)
Chinese (zh)
Inventor
武田贵成
林哲太郎
二阶堂爱
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
RIKEN
Original Assignee
Toyobo Co Ltd
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd, RIKEN filed Critical Toyobo Co Ltd
Publication of CN120584195A publication Critical patent/CN120584195A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202480008982.0A 2023-01-26 2024-01-22 源自核糖体rna的逆转录的抑制 Pending CN120584195A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2023010502 2023-01-26
JP2023-010502 2023-01-26
PCT/JP2024/001631 WO2024157925A1 (ja) 2023-01-26 2024-01-22 リボソーマルrna由来の逆転写の抑制

Publications (1)

Publication Number Publication Date
CN120584195A true CN120584195A (zh) 2025-09-02

Family

ID=91970683

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202480008982.0A Pending CN120584195A (zh) 2023-01-26 2024-01-22 源自核糖体rna的逆转录的抑制

Country Status (4)

Country Link
EP (1) EP4656735A1 (https=)
JP (1) JPWO2024157925A1 (https=)
CN (1) CN120584195A (https=)
WO (1) WO2024157925A1 (https=)

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2688155C (en) * 2007-05-31 2020-02-11 The Regents Of The University Of California High specificity and high sensitivity detection based on steric hindrance & enzyme-related signal amplification
WO2009055732A1 (en) 2007-10-26 2009-04-30 Rosetta Inpharmatics Llc Cdna synthesis using non-random primers
ES2539856T3 (es) 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
PL3892295T3 (pl) 2011-05-24 2023-07-24 BioNTech SE Zindywidualizowane szczepionki przeciwnowotworowe
WO2016052619A1 (ja) 2014-09-30 2016-04-07 国立研究開発法人理化学研究所 核酸の増幅方法
JP6439019B1 (ja) 2017-08-24 2018-12-19 積水化成品工業株式会社 ハイドロゲル、その用途及びその製造方法
US20220154180A1 (en) 2019-03-13 2022-05-19 Toyobo Co., Ltd. Production and amplification of nucleic acids
JP7532820B2 (ja) * 2019-03-20 2024-08-14 東洋紡株式会社 非特異増幅が抑制された核酸増幅法
JP2021153393A (ja) * 2020-03-25 2021-10-07 東洋紡株式会社 核酸増幅反応用組成物
WO2022024935A1 (ja) * 2020-07-29 2022-02-03 東洋紡株式会社 非特異的な核酸増幅を抑制する方法
CN116529362A (zh) * 2020-07-29 2023-08-01 东洋纺株式会社 经改良的病毒的检测方法
JP7713682B2 (ja) * 2021-09-06 2025-07-28 東洋紡株式会社 長時間の核酸増幅反応の改善

Also Published As

Publication number Publication date
WO2024157925A1 (ja) 2024-08-02
EP4656735A1 (en) 2025-12-03
JPWO2024157925A1 (https=) 2024-08-02

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