CN120265969A - 冷冻装置、显微镜、冷冻方法、以及观察方法 - Google Patents

冷冻装置、显微镜、冷冻方法、以及观察方法 Download PDF

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Publication number
CN120265969A
CN120265969A CN202380081812.0A CN202380081812A CN120265969A CN 120265969 A CN120265969 A CN 120265969A CN 202380081812 A CN202380081812 A CN 202380081812A CN 120265969 A CN120265969 A CN 120265969A
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CN
China
Prior art keywords
sample
liquid
cryogen
refrigerant
freezing
Prior art date
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Pending
Application number
CN202380081812.0A
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English (en)
Chinese (zh)
Inventor
藤田克昌
山中真仁
熊本康昭
十康介
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University of Osaka NUC
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Osaka University NUC
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Filing date
Publication date
Application filed by Osaka University NUC filed Critical Osaka University NUC
Publication of CN120265969A publication Critical patent/CN120265969A/zh
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/28—Base structure with cooling device
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/20—Heating; Cooling
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/22—Means for packing or storing viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0088—Inverse microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/30—Base structure with heating device
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Microscoopes, Condenser (AREA)
CN202380081812.0A 2022-11-29 2023-10-27 冷冻装置、显微镜、冷冻方法、以及观察方法 Pending CN120265969A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2022-189880 2022-11-29
JP2022189880 2022-11-29
JP2023-122175 2023-07-27
JP2023122175 2023-07-27
PCT/JP2023/038854 WO2024116681A1 (ja) 2022-11-29 2023-10-27 凍結装置、顕微鏡、凍結方法、及び観察方法

Publications (1)

Publication Number Publication Date
CN120265969A true CN120265969A (zh) 2025-07-04

Family

ID=91323729

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380081812.0A Pending CN120265969A (zh) 2022-11-29 2023-10-27 冷冻装置、显微镜、冷冻方法、以及观察方法

Country Status (4)

Country Link
EP (1) EP4628868A4 (https=)
JP (1) JPWO2024116681A1 (https=)
CN (1) CN120265969A (https=)
WO (1) WO2024116681A1 (https=)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59218417A (ja) * 1983-05-25 1984-12-08 Osaka Oxgen Ind Ltd 極低温検鏡法およびそれに用いる顕微鏡
JPS61263037A (ja) * 1985-05-16 1986-11-21 Univ Kyoto 凍結試料交換装置
JPH0725663Y2 (ja) * 1989-06-02 1995-06-07 株式会社ケット科学研究所 測定機用試料皿
JPH03134527A (ja) * 1989-10-19 1991-06-07 Osaka Gas Co Ltd 極微小量の試料の温度測定装置
GB0420657D0 (en) * 2004-09-16 2004-10-20 Thermo Shandon Ltd Cryostat and microtome
JP2007080517A (ja) * 2005-09-09 2007-03-29 Jeol Ltd 電子顕微鏡用クライオステージ
DE102012013267A1 (de) * 2012-07-04 2014-01-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Substrateinrichtung, Konservierungsgerät und Verfahren zur Kryokonservierung einer biologischen Probe
EP2853847B1 (en) * 2013-09-30 2016-02-03 Fei Company Preparation of cryogenic sample for charged-particle microscopy
JP6314051B2 (ja) 2014-05-29 2018-04-18 パナソニック インテレクチュアル プロパティ コーポレーション オブ アメリカＰａｎａｓｏｎｉｃ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ Ｃｏｒｐｏｒａｔｉｏｎ ｏｆ Ａｍｅｒｉｃａ 空調機器を遠隔制御する端末装置の制御方法、端末装置が実行するプログラム及び端末装置が実行するレコメンド方法
EP3179229B1 (en) * 2015-12-11 2019-01-30 FEI Company Preparation of cryogenic sample for charged-particle microscopy
US10481055B2 (en) * 2016-08-26 2019-11-19 Simple Origin, Inc. System and method for refilling cryogen in microscope cryogen holders
US20240201056A1 (en) * 2021-04-27 2024-06-20 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method and device for ultra-rapid cryo-fixation of a sample for microscopic studies
CN117642669A (zh) * 2021-05-12 2024-03-01 国立大学法人大阪大学 显微镜和观察方法
JP7759280B2 (ja) 2022-02-22 2025-10-23 Ｊｆｅ建材株式会社 デッキプレートの製造方法

Also Published As

Publication number Publication date
EP4628868A1 (en) 2025-10-08
WO2024116681A1 (ja) 2024-06-06
EP4628868A4 (en) 2026-04-01
JPWO2024116681A1 (https=) 2024-06-06

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