CN119013403A - L-核酸适体的镜像选择 - Google Patents

L-核酸适体的镜像选择 Download PDF

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Publication number
CN119013403A
CN119013403A CN202380032837.1A CN202380032837A CN119013403A CN 119013403 A CN119013403 A CN 119013403A CN 202380032837 A CN202380032837 A CN 202380032837A CN 119013403 A CN119013403 A CN 119013403A
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CN
China
Prior art keywords
dna
nucleic acid
aptamer
sequencing
aptamers
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Pending
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CN202380032837.1A
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English (en)
Chinese (zh)
Inventor
朱听
陈季
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Tsinghua University
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Tsinghua University
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Publication of CN119013403A publication Critical patent/CN119013403A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202380032837.1A 2022-02-03 2023-02-02 L-核酸适体的镜像选择 Pending CN119013403A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263306139P 2022-02-03 2022-02-03
US63/306139 2022-02-03
US202263311092P 2022-02-17 2022-02-17
US63/311092 2022-02-17
PCT/IB2023/050908 WO2023148646A1 (en) 2022-02-03 2023-02-02 Mirror-image selection of l-nucleic acid aptamers

Publications (1)

Publication Number Publication Date
CN119013403A true CN119013403A (zh) 2024-11-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380032837.1A Pending CN119013403A (zh) 2022-02-03 2023-02-02 L-核酸适体的镜像选择

Country Status (7)

Country Link
US (1) US20250027090A1 (https=)
EP (1) EP4473115A1 (https=)
JP (1) JP2025507269A (https=)
CN (1) CN119013403A (https=)
AU (1) AU2023216497A1 (https=)
CA (1) CA3243286A1 (https=)
WO (1) WO2023148646A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119757294A (zh) * 2024-12-20 2025-04-04 厦门市亮惠诊断技术有限公司 一种用于d-乳酸高灵敏检测的l-核酸适体传感器

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017028548A1 (zh) * 2015-08-18 2017-02-23 清华大学 镜像核酸复制体系
CN111057132A (zh) * 2019-10-16 2020-04-24 四川农业大学 牛病毒性腹泻病毒e0蛋白氨基酸及制备方法
US20200385803A1 (en) * 2018-02-28 2020-12-10 Roche Sequencing Solutions, Inc. Tagged nucleoside compounds useful for nanopore detection
CN112105748A (zh) * 2018-04-05 2020-12-18 清华大学 测序和生产核酸序列的方法

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US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US20070141598A1 (en) * 2005-02-09 2007-06-21 Pacific Biosciences Of California, Inc. Nucleotide Compositions and Uses Thereof
WO2013170963A2 (en) 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids
TWI731028B (zh) * 2016-01-22 2021-06-21 國立大學法人東京大學 用以篩選核酸適體之方法
WO2019236571A1 (en) 2018-06-04 2019-12-12 Chan Zuckerberg Biohub, Inc. Compositions and methods involving non-natural aptamer libraries

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017028548A1 (zh) * 2015-08-18 2017-02-23 清华大学 镜像核酸复制体系
US20200385803A1 (en) * 2018-02-28 2020-12-10 Roche Sequencing Solutions, Inc. Tagged nucleoside compounds useful for nanopore detection
CN112105748A (zh) * 2018-04-05 2020-12-18 清华大学 测序和生产核酸序列的方法
CN111057132A (zh) * 2019-10-16 2020-04-24 四川农业大学 牛病毒性腹泻病毒e0蛋白氨基酸及制备方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEN J, CHEN M, ZHU TF.: "Directed evolution and selection of biostable L-DNA aptamers with a mirror-image DNA polymerase", 《NAT BIOTECHNOL.》, 6 June 2022 (2022-06-06), pages 1601 - 1609 *
FAN C, DENG Q, ZHU TF.: "Bioorthogonal information storage in L-DNA with a high-fidelity mirror-image Pfu DNA polymerase", 《NAT BIOTECHNOL.》, vol. 39, no. 12, 29 July 2021 (2021-07-29), pages 1548 - 1555, XP037639832, DOI: 10.1038/s41587-021-00969-6 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119757294A (zh) * 2024-12-20 2025-04-04 厦门市亮惠诊断技术有限公司 一种用于d-乳酸高灵敏检测的l-核酸适体传感器

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Publication number Publication date
JP2025507269A (ja) 2025-03-18
EP4473115A1 (en) 2024-12-11
US20250027090A1 (en) 2025-01-23
AU2023216497A1 (en) 2024-08-15
CA3243286A1 (en) 2023-08-10
WO2023148646A1 (en) 2023-08-10

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