CA3243286A1 - L-NUCLEIC ACID APTAMER MIRROR IMAGE SELECTION - Google Patents

L-NUCLEIC ACID APTAMER MIRROR IMAGE SELECTION

Info

Publication number
CA3243286A1
CA3243286A1 CA3243286A CA3243286A CA3243286A1 CA 3243286 A1 CA3243286 A1 CA 3243286A1 CA 3243286 A CA3243286 A CA 3243286A CA 3243286 A CA3243286 A CA 3243286A CA 3243286 A1 CA3243286 A1 CA 3243286A1
Authority
CA
Canada
Prior art keywords
mirror
nucleic acid
image selection
acid aptamers
aptamers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3243286A
Other languages
English (en)
French (fr)
Inventor
Ting Zhu
Ji Chen
Original Assignee
Tsinghua University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tsinghua University filed Critical Tsinghua University
Publication of CA3243286A1 publication Critical patent/CA3243286A1/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1048SELEX
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/16Aptamers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00Production
    • C12N2330/30Production chemically synthesised
    • C12N2330/31Libraries, arrays

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3243286A 2022-02-03 2023-02-02 L-NUCLEIC ACID APTAMER MIRROR IMAGE SELECTION Pending CA3243286A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263306139P 2022-02-03 2022-02-03
US63/306,139 2022-02-03
US202263311092P 2022-02-17 2022-02-17
US63/311,092 2022-02-17
PCT/IB2023/050908 WO2023148646A1 (en) 2022-02-03 2023-02-02 Mirror-image selection of l-nucleic acid aptamers

Publications (1)

Publication Number Publication Date
CA3243286A1 true CA3243286A1 (en) 2023-08-10

Family

ID=85380927

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3243286A Pending CA3243286A1 (en) 2022-02-03 2023-02-02 L-NUCLEIC ACID APTAMER MIRROR IMAGE SELECTION

Country Status (7)

Country Link
US (1) US20250027090A1 (https=)
EP (1) EP4473115A1 (https=)
JP (1) JP2025507269A (https=)
CN (1) CN119013403A (https=)
AU (1) AU2023216497A1 (https=)
CA (1) CA3243286A1 (https=)
WO (1) WO2023148646A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119757294B (zh) * 2024-12-20 2025-08-29 厦门市亮惠诊断技术有限公司 一种用于d-乳酸高灵敏检测方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US20070141598A1 (en) * 2005-02-09 2007-06-21 Pacific Biosciences Of California, Inc. Nucleotide Compositions and Uses Thereof
WO2013170963A2 (en) 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids
CN106467910A (zh) * 2015-08-18 2017-03-01 清华大学 L-dna/l-rna聚合酶及其应用
TWI731028B (zh) * 2016-01-22 2021-06-21 國立大學法人東京大學 用以篩選核酸適體之方法
WO2019166457A1 (en) * 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Tagged nucleoside compounds useful for nanopore detection
CA3094598A1 (en) * 2018-04-05 2019-10-10 Tsinghua University Methods of sequencing and producing nucleic acid sequences
WO2019236571A1 (en) 2018-06-04 2019-12-12 Chan Zuckerberg Biohub, Inc. Compositions and methods involving non-natural aptamer libraries
CN111057132B (zh) * 2019-10-16 2022-01-07 四川农业大学 牛病毒性腹泻病毒e0蛋白氨基酸及制备方法

Also Published As

Publication number Publication date
JP2025507269A (ja) 2025-03-18
EP4473115A1 (en) 2024-12-11
US20250027090A1 (en) 2025-01-23
AU2023216497A1 (en) 2024-08-15
CN119013403A (zh) 2024-11-22
WO2023148646A1 (en) 2023-08-10

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